Standard Curve (Sample)
Rat RAGE ELISA Kit | AGER elisa kit
Rat RAGE PicoKine ELISA Kit
Gene Names
Ager; RAGE
Reactivity
Rat
Synonyms
RAGE; N/A; Rat RAGE PicoKine ELISA Kit; Advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor; Advanced glycosylation end product specific receptor; Ager; DAMA 358M23.4; LE 9211 A antigen; MAPK MAK MRK overlapping kinase; MGC22357; MOK; OTTHUMP00000174885; RAGE 1; RAGE_HUMAN; RAGE1; Receptor for advanced glycation end products; Receptor for advanced glycosylation end products; Renal tumor antigen; Renal tumor antigen 1; AGER elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat Ager
Sequence Length
402
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
78 pg/ml - 5,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for AGER elisa kit
Principle of the Assay: The Picokine™ Rat Ager Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Rat Ager with a 96-well strip plate that is pre-coated with antibody specific for Ager. The detection antibody is a biotinylated antibody specific for Ager. The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat Ager with immunogen: Expression system for standard: NS0; Immunogen sequence: Q24-A342. The kit is analytically validated with ready-to-use reagents. To measure Rat Ager, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Rat Ager in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ager in the sample.
Background/Introduction: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as "AGER". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
Background/Introduction: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as "AGER". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
42,664 Da
NCBI Official Full Name
Advanced glycosylation end product-specific receptor
NCBI Official Synonym Full Names
advanced glycosylation end product-specific receptor
NCBI Official Symbol
Ager
NCBI Official Synonym Symbols
RAGE
NCBI Protein Information
advanced glycosylation end product-specific receptor; receptor for advanced glycosylation end products; advanced glycosylation end product-specific receptor variant 2; advanced glycosylation end product-specific receptor variant 3; advanced glycosylation end product-specific receptor variant 4; advanced glycosylation end product-specific receptor variant 5
UniProt Protein Name
Advanced glycosylation end product-specific receptor
UniProt Gene Name
Ager
UniProt Synonym Gene Names
Rage
UniProt Entry Name
RAGE_RAT
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Product Notes
The Rat AGER ager (Catalog #AAA45943) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45943 ELISA Kit recognizes Rat AGER. It is sometimes possible for the material contained within the vial of "RAGE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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