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Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD3:ALEXA 647)

CD3, Monoclonal Antibody (Cat# AAA26780)

Full Name

CD3 (MaxLight 405)

Reactivity

Human

Applications

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)

Purity

Purified by Protein G Affinity Chromatography

Pricing

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CD45, Monoclonal Antibody (Cat# AAA28060)

Full Name

CD45 Monoclonal Antibody

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

>95%, Protein A purified

Pricing

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IF (Immunofluorescence) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

Cleaved-Caspase-1, Polyclonal Antibody (Cat# AAA28828)

Full Name

Cleaved-Caspase-1 (D210) Polyclonal Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CD34 at 1:1000 dilution.)

CD34, Polyclonal Antibody (Cat# AAA30620)

Full Name

CD34 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis extracts of 293T cells, using MonoMethyl-Histone H3-K4 antibody and rabbit IgG. P1 and P2 were located on promoter (GAPDH). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

H3K4me1, Antibody (Cat# AAA10653)

Full Name

Histone H3K4me1 Polyclonal Antibody

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP, CHIPseq

Purity

Affinity Purification

Pricing

Apelin, AP, ELISA Kit (Cat# AAA11165)

Full Name

Human Apelin, AP ELISA Kit

Gene Names

APLN; APEL; XNPEP2

Reactivity

Human

Pricing

soluble Interleukin 6 Receptor (sIL-6R), ELISA Kit (Cat# AAA12445)

Full Name

Rat soluble Interleukin 6 Receptor (sIL-6R) ELISA Kit

Gene Names

IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1

Reactivity

Rat

Pricing

Granzyme B (Gzms-B), ELISA Kit (Cat# AAA12701)

Full Name

Porcine Granzyme B (Gzms-B) ELISA Kit

Gene Names

GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

Reactivity

Porcine

Pricing

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Application Data (Ref. 3: Isolation, culture and immunocytochemical characterization of HUCPC. The dissection under sterile condition of foetal and full-term cords was performed to expose the WJ, the vein and arteries (A). After the in vitro expansion of foetal HUCPC (B) the cells ..)

Chick Embryo Extract, Ultrafiltrate, Reagent (Cat# AAA14749)

Full Name

Chick Embryo Extract, Ultrafiltrate (CEE)

Purity

Molecular Biology Grade

Pricing

microtubule-associated protein 2, ELISA Kit (Cat# AAA15517)

Full Name

Human microtubule-associated protein 2, MAP-2 ELISA Kit

Gene Names

MAP2; MAP2A; MAP2B; MAP2C

Reactivity

Human

Pricing

Anti double stranded DNA, ELISA Kit (Cat# AAA16285)

Full Name

Human Anti double stranded DNA ELISA Kit

Reactivity

Human

Pricing

Protein Tyrosine Phosphatase 1B, Active Protein (Cat# AAA14754)

Full Name

Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)

Gene Names

PTPN1; PTP1B

Purity

Highly Purified
>95% by SDS PAGE; Purified by using conventional chromatography techniques.

Pricing

Heat Shock Protein 70, HSP-70, ELISA Kit (Cat# AAA15266)

Full Name

Pig Heat Shock Protein 70, HSP-70 ELISA Kit

Reactivity

Pig

Pricing

High molecular weight Adiponectin, ELISA Kit (Cat# AAA17058)

Full Name

Rat High molecular weight Adiponectin ELISA Kit

Reactivity

Rat

Pricing

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Kynurenine 3-monooxygenase, Polyclonal Antibody (Cat# AAA18850)

Full Name

Rabbit anti-human Kynurenine 3-monooxygenase polyclonal Antibody(KMO)

Gene Names

KMO; dJ317G22.1

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of Raji cells using anti-PSMD14 antibody (AAA19874).Overlay histogram showing Raji cells stained with AAA19874 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD14 Antibody (AAA19874, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of POH1/PSMD14 using anti-POH1/PSMD14 antibody (AAA19874).POH1/PSMD14 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-POH1/PSMD14 Antibody (AAA19874) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human HT-1080 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POH1/PSMD14 antigen affinity purified polyclonal antibody (#AAA19874) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POH1/PSMD14 at approximately 36 kDa. The expected band size for POH1/PSMD14 is at 35 kDa.)

POH1/PSMD14, Polyclonal Antibody (Cat# AAA19874)

Full Name

Anti-POH1/PSMD14 Antibody Picoband

Gene Names

PSMD14; PAD1; POH1; RPN11

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

Receptor I For The Fc Region Of Immunoglobulin G (FcgRI), ELISA Kit (Cat# AAA20386)

Full Name

Human Receptor I For The Fc Region Of Immunoglobulin G (FcgRI) ELISA Kit

Gene Names

LOC101087936; FcgRI

Reactivity

Human

Pricing

SPARC-Related Modular Calcium-Binding Protein 1, ELISA Kit (Cat# AAA23714)

Full Name

Human SPARC-Related Modular Calcium-Binding Protein 1 ELISA Kit

Reactivity

Human

Pricing

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WB (Western Blot) (GNRHR2 monoclonal antibody, Western Blot analysis of GNRHR2 expression in HeLa.)

GNRHR2, Monoclonal Antibody (Cat# AAA24226)

Full Name

GNRHR2 (Putative Gonadotropin-releasing Hormone II Receptor, GnRH II Receptor, GnRH-II-R, Type II GnRH Receptor) (AP)

Gene Names

FZD2; Fz2; fz-2; fzE2; hFz2; OMOD2

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in human colon.)

SMAD3, Monoclonal Antibody (Cat# AAA25250)

Full Name

SMAD3 (Mothers Against Decapentaplegic Homolog 3, SMAD 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2, hSMAD3, MADH3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, MGC60396) (FITC)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged NDUFA9 is ~0.1ng/ml as a capture antibody.)

NDUFA9, Monoclonal Antibody (Cat# AAA25762)

Full Name

NDUFA9 (NADH Dehydrogenase [Ubiquinone] 1 alpha Subcomplex Subunit 9, Mitochondrial, NADH-Ubiquinone Oxidoreductase 39kD Subunit, Complex I-39kD, CI-39kD, NDUFS2L, MGC111043) (PE)

Gene Names

NDUFA9; CC6; CI39k; CI-39k; MC1DN26; NDUFS2L; SDR22E1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STX4 on HeLa cell. [antibody concentration 10 ug/ml])

STX4A, Monoclonal Antibody (Cat# AAA26530)

Full Name

STX4A (Syntaxin 4, STX4A, p35-2) (HRP)

Gene Names

STX4; STX4A; p35-2

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

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Application Data (Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)

EMR1, Monoclonal Antibody (Cat# AAA26786)

Full Name

EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (MaxLight 405)

Reactivity

Mouse

Applications

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blot (WB)

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

HMGB1 (High mobility group protein B1), ELISA Kit (Cat# AAA27554)

Full Name

Porcine HMGB1 (High mobility group protein B1) ELISA Kit

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Reactivity

Porcine

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

p53, Polyclonal Antibody (Cat# AAA29090)

Full Name

p53 Polyclonal Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using BRMS1 at dilution of 1:100 (40x lens).)

BRMS1, Polyclonal Antibody (Cat# AAA30626)

Full Name

BRMS1 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

DUSP6, Monoclonal Antibody (Cat# AAA24490)

Full Name

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) APC

Gene Names

DUSP6; HH19; MKP3; PYST1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (CAPNS1 monoclonal antibody, Western Blot analysis of CAPNS1 expression in A-431.)

CAPNS1, Monoclonal Antibody (Cat# AAA24746)

Full Name

CAPNS1 (Calpain Small Subunit 1, CSS1, Calcium-activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calcium-dependent Protease Small Subunit, CDPS, Calcium-dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CAPN4, CAPNS) (Biotin)

Gene Names

CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4

Reactivity

Human, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)

ABHD5, Monoclonal Antibody (Cat# AAA25002)

Full Name

ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase Domain-containing Protein 5, Lipid Droplet-binding Protein CGI-58, CGI-58, MGC8731, NCIE2) (FITC)

Gene Names

ABHD5; CDS; CGI58; IECN2; NCIE2

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (RAB33B monoclonal antibody. Western Blot analysis of RAB33B expression in NIH/3T3.)

RAB33B, Monoclonal Antibody (Cat# AAA25514)

Full Name

RAB33B (Ras-related Protein Rab-33B, SMC2) (HRP)

Gene Names

RAB33B; SMC2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml])

MAPK9, Monoclonal Antibody (Cat# AAA26538)

Full Name

MAPK9 (Mitogen-Activated Protein Kinase 9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK) (HRP)

Gene Names

MAPK9; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

Calprotectin (Calprotectin), ELISA Kit (Cat# AAA27562)

Full Name

Mouse Calprotectin (Calprotectin) ELISA Kit

Reactivity

Mouse

Pricing

Phosphofructokinase, Assay Kit (Cat# AAA27818)

Full Name

Phosphofructokinase Microplate Assay Kit

Gene Names

Pfk; BcDNA.GH12192; BcDNA:GH12192; CG4001; DmelCG4001; l(2)06339; pfk; PFK; PFK1

Reactivity

General

Applications

Functional Assay

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CDK6, Polyclonal Antibody (Cat# AAA28330)

Full Name

CDK6 Rabbit pAb

Gene Names

CDK6; PLSTIRE

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

Integrin alpha1, Polyclonal Antibody (Cat# AAA29354)

Full Name

Integrin alpha1 Polyclonal Antibody

Gene Names

ITGA1; VLA1; CD49a

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using MMP25 at dilution of 1:100 (40x lens).)

MMP25, Polyclonal Antibody (Cat# AAA30634)

Full Name

MMP25 Rabbit Polyclonal Antibody

Gene Names

MMP25; MMP20; MMPL1; MMP-25; MMP20A; MT6MMP; MTMMP6; MT-MMP6; MT6-MMP; MT-MMP 6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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Claudin 1, Monoclonal Antibody (Cat# AAA30890)

Full Name

Claudin 1 (ABT-CLD1) Antibody

Gene Names

CLDN1; CLD1; SEMP1; ILVASC

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)

Purity

Protein G

Pricing

Regenerating Islet Derived Protein 1 Alpha (REG1a), ELISA Kit (Cat# AAA11179)

Full Name

Human Regenerating Islet Derived Protein 1 Alpha (REG1a) ELISA Kit

Gene Names

REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1

Reactivity

Human

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U20S cells using anti-NFIA antibody (AAA11691).Overlay histogram showing U20S cells stained with AAA11691 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (AAA11691,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)