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DRD1, Polyclonal Antibody (Cat# AAA29653)

Full Name

DRD1 Antibody

Gene Names

DRD1; DADR; DRD1A

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

N4BP1, Polyclonal Antibody (Cat# AAA29078)

Full Name

N4BP1 Polyclonal Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

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TOPBP1, Polyclonal Antibody (Cat# AAA31285)

Full Name

Phospho-TOPBP1 (Ser1138) Antibody

Gene Names

TOPBP1; TOP2BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

MARK2, Polyclonal Antibody (Cat# AAA31392)

Full Name

Phospho-MARK2 (Thr596) Antibody

Gene Names

MARK2; EMK1; EMK-1; PAR-1; Par1b; Par-1b

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

Intercellular Adhesion Molecule 1, sICAM-1, ELISA Kit (Cat# AAA11091)

Full Name

Rat Soluble Intercellular Adhesion Molecule 1, sICAM-1 ELISA Kit

Gene Names

ICAM1; BB2; CD54; P3.58

Reactivity

Rat

Pricing

tartrate resistant acid phosphatase 5b (TRACP-5b), ELISA Kit (Cat# AAA12666)

Full Name

Rat tartrate resistant acid phosphatase 5b (TRACP-5b) ELISA Kit

Gene Names

ACP5; TRAP; SPENCDI

Reactivity

Rat

Pricing

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IHC (Immunohistochemistry) (Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

ZC3H12A, Polyclonal Antibody (Cat# AAA11042)

Full Name

ZC3H12A (CT) Antibody

Gene Names

ZC3H12A; MCPIP; MCPIP1; dJ423B22.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

ZC3H12A Antibody is affinity chromatography purified via peptide column.

Pricing

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IF (Immunofluorescence) (Immunofluorescence of Clusterin in mouse brain tissue with Clusterin Antibody at 20 μg/mL.Green: Clusterin Antibody (3856)Red: Phylloidin stainingBlue: DAPI staining)

Clusterin, Polyclonal Antibody (Cat# AAA10914)

Full Name

Clusterin Antibody

Gene Names

CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Clusterin Antibody is affinity chromatography purified via peptide column.

Pricing

alpha 1 Antitrypsin, Polyclonal Antibody (Cat# AAA14320)

Full Name

alpha 1 Antitrypsin antibody

Gene Names

SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT

Reactivity

Human

Applications

Immunohistochemistry

Pricing

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WB (Western Blot)

GAA, Monoclonal Antibody (Cat# AAA19211)

Full Name

Anti-GAA Antibody (Monoclonal, 2G7)

Gene Names

GAA; LYAG

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (SF3B2 monoclonal antibody . Western Blot analysis of SF3B2 expression in PC-12.)

SF3B2, Monoclonal Antibody (Cat# AAA24082)

Full Name

SF3B2 (Splicing Factor 3B Subunit 2, Pre-mRNA-splicing Factor SF3b 145 kDa Subunit, SF3b145, SF3b150, Spliceosome-associated Protein 145, SAP 145, SAP145)

Gene Names

SF3B2; Cus1; SF3b1; SAP145; SF3B145; SF3b150

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Vimentin Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

Vimentin, Polyclonal Antibody (Cat# AAA22322)

Full Name

Vimentin Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

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WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)

TIMM9, Monoclonal Antibody (Cat# AAA24683)

Full Name

TIMM9 (Mitochondrial Import Inner Membrane Translocase Subunit Tim9, TIM9, TIM9A, TIMM9A) APC

Gene Names

TIMM9; TIM9; TIM9A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)

Tubulin alpha-4A Chain, Monoclonal Antibody (Cat# AAA24691)

Full Name

Tubulin alpha-4A Chain (TUBA4A, Alpha-tubulin 1, Tubulin alpha-1 Chain, TUBA1, Testis-specific alpha-tubulin, Tubulin H2-alpha) APC

Gene Names

TUBA4A; ALS22; TUBA1; H2-ALPHA

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (MARCH7 monoclonal antibody. Western Blot analysis of 'MARCH7 expression in PC-12.)

2019-03-07, Monoclonal Antibody (Cat# AAA25154)

Full Name

MARCH7 (E3 Ubiquitin-protein Ligase MARCH7, Axotrophin, Membrane-associated RING Finger Protein 7, Membrane-associated RING-CH Protein VII, MARCH-VII, RING Finger Protein 177, AXOT, RNF177, DKFZp586F1122) (FITC)

Gene Names

MARCH7; AXO; AXOT; RNF177; MARCH-VII

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

PIK3R1, Monoclonal Antibody (Cat# AAA24907)

Full Name

PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (Biotin)

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

RBM9, Monoclonal Antibody (Cat# AAA24929)

Full Name

RBM9 (RNA Binding Motif Protein 9, RNA Binding Protein 9, Hexaribonucleotide Binding Protein 2, HRNBP2, Repressor of Tamoxifen Transcriptional Activity, RTA) (Biotin)

Gene Names

RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody. Lane 1: CTNNB1 transfected lysate (85.5kD). Lane 2: Non-transfected lysate.)

CTNNB1, Monoclonal Antibody (Cat# AAA25658)

Full Name

CTNNB1 (Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286) (PE)

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; NEDSDV; armadillo

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Detection limit for recombinant GST tagged PGR is approximately 0.1ng/ml as a capture antibody.)

PGR, Monoclonal Antibody (Cat# AAA26191)

Full Name

PGR (Progesterone Receptor, NR3C3, PR) (Biotin)

Gene Names

PGR; PR; NR3C3

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (Western Blot analysis of LYPLA3 expression in NIH/3T3)

LYPLA3, Monoclonal Antibody (Cat# AAA25738)

Full Name

LYPLA3 (PLA2G15, Group XV Phospholipase A2, 1-O-acylceramide Synthase, ACS, LCAT-like Lysophospholipase, LLPL, Lysophospholipase 3, Lysosomal Phospholipase A2, LPLA2, UNQ341/PRO540) (PE)

Gene Names

PLA2G15; ACS; LLPL; LPLA2; LYPLA3; GXVPLA2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in PC-12.)

TAB1, Monoclonal Antibody (Cat# AAA25860)

Full Name

TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, MAP3K7IP1) (PE)

Gene Names

TAB1; 3'-Tab1; MAP3K7IP1

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26898)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry

Purity

Purified by Immunoaffinity chromatography.

Pricing

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WB (Western Blot) (MEOX2 monoclonal antibody (M02), clone 5A6. Western Blot analysis of MEOX2 expression in PC-12 (Cat # L012V1).)

MEOX2, Monoclonal Antibody (Cat# AAA26501)

Full Name

MEOX2 (Mesenchyme Homeobox 2, GAX, MOX2) (HRP)

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4. Western Blot analysis of NME2 expression in PC-12 (Cat # L012V1).)

NME2, Monoclonal Antibody (Cat# AAA26506)

Full Name

NME2 (Non-Metastatic Cells 2, Protein (NM23B) Expressed in, MGC111212, NDPK-B, NDPKB, NM23-H2, NM23B, puf) (HRP)

Gene Names

NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

DGAT2L3, Polyclonal Antibody (Cat# AAA29011)

Full Name

DGAT2L3 Polyclonal Antibody

Gene Names

AWAT1; DGA2; DGAT2L3

Reactivity

Human

Applications

Western Blot

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB: 1:3000 IP:1:200 IHC 1:50-300)

CA IX, Monoclonal Antibody (Cat# AAA28876)

Full Name

CA IX Monoclonal Antibody (12F10)

Gene Names

CA9; MN; CAIX

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Pricing

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EB3, Monoclonal Antibody (Cat# AAA30433)

Full Name

EB3 Antibody

Gene Names

MAPRE3; EB3; RP3; EBF3; EBF3-S

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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53BP1, Monoclonal Antibody (Cat# AAA30337)

Full Name

53BP1 Antibody

Gene Names

TP53BP1; p202; 53BP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with gamma Synuclein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

gamma Synuclein, Monoclonal Antibody (Cat# AAA30372)

Full Name

gamma Synuclein Antibody

Gene Names

SNCG; SR; BCSG1

Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of Hela cell. 1.CREB-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). LC3A mouse Monoclonal Antibody(5G10)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

LC3A, Monoclonal Antibody (Cat# AAA30831)

Full Name

LC3A Mouse Monoclonal Antibody (5G10)

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

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Catalase, Polyclonal Antibody (Cat# AAA29962)

Full Name

Catalase Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Peptide affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CDK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

CDK1, Monoclonal Antibody (Cat# AAA30031)

Full Name

CDK1 Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

NLRX1, Polyclonal Antibody (Cat# AAA29337)

Full Name

NLRX1 Polyclonal Antibody

Gene Names

NLRX1; NOD5; NOD9; NOD26; DLNB26; CLR11.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

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beta Arrestin 1, Monoclonal Antibody (Cat# AAA30095)

Full Name

beta Arrestin 1 Antibody

Gene Names

ARRB1; ARB1; ARR1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

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IHC (Immunohistchemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

BAG3, Polyclonal Antibody (Cat# AAA31421)

Full Name

Phospho-BAG3 (Tyr457) Antibody

Gene Names

BAG3; BIS; BAG-3; CAIR-1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

Soluble triggering receptor expresses on myeloid cells-2, ELISA Kit (Cat# AAA22588)

Full Name

Mouse soluble triggering receptor expresses on myeloid cells-2 (sTREM-2) ELISA Kit

Reactivity

Mouse

Pricing

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Azide Free)

CD38, Monoclonal Antibody (Cat# AAA12062)

Full Name

MOUSE ANTI HUMAN CD38

Gene Names

CD38; T10; ADPRC 1

Applications

Immunohistochemistry, Flow Cytometry

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-PCCA antibody (AAA19790).Overlay histogram showing HepG2 cells stained with AAA19790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCCA Antibody (AAA19790, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PCCA using anti-PCCA antibody (AAA19790).PCCA was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat liver tissue lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCA antigen affinity purified polyclonal antibody (#AAA19790) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCCA at approximately 70 kDa. The expected band size for PCCA is at 80 kDa.)

PCCA, Polyclonal Antibody (Cat# AAA19790)

Full Name

Anti-PCCA Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MFAP3 antibody (AAA19989).Overlay histogram showing 293T cells stained with AAA19989 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MFAP3 Antibody (AAA19989, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MFAP3 using anti-MFAP3 antibody (AAA19989).MFAP3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MFAP3 Antibody (AAA19989) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFAP3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MFAP3 at approximately 45-50 kDa. The expected band size for MFAP3 is at 40 kDa.)

MFAP3, Polyclonal Antibody (Cat# AAA19989)

Full Name

Anti-MFAP3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Figure 6. IF analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (AAA19508).CBX1/HP1 beta was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-CBX1/HP1 beta Antibody (AAA19508) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

CBX1/HP1 beta, Polyclonal Antibody (Cat# AAA19508)

Full Name

Anti-CBX1/HP1 beta Antibody Picoband

Gene Names

CBX1; CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Immunogen affinity purified.

Pricing

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Application Data (Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.)

PEPD, Monoclonal Antibody (Cat# AAA24604)

Full Name

PEPD (Xaa-Pro Dipeptidase, X-Pro Dipeptidase, Proline Dipeptidase, Prolidase, Imidodipeptidase, Peptidase D, PRD, MGC10905) APC

Gene Names

PEPD; PROLIDASE

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

MCM3, Monoclonal Antibody (Cat# AAA25980)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (AP)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Western Blot

Purity

Purified

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Chitinase, Assay Kit (Cat# AAA27826)

Full Name

Chitinase Microplate Assay Kit

Reactivity

General

Applications

Functional Assay

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High Density Lipoprotein (HDL), ELISA Kit (Cat# AAA12628)

Full Name

Mouse High Density Lipoprotein (HDL) ELISA Kit

Reactivity

Mouse

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insulin-like growth factor binding protein 7, ELISA Kit (Cat# AAA15846)

Full Name

Human Insulin-like growth factor-binding protein 7, IGFBP7/MAC25/PSF ELISA Kit

Gene Names

IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1

Reactivity

Human

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin embedded mouse heart using TPM2 antibody (AAA10708) at dilution of 1:100 (40xlens).)

TPM2, Polyclonal Antibody (Cat# AAA10708)

Full Name

TPM2 Polyclonal Antibody

Gene Names

TPM2; DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

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F4/80, Monoclonal Antibody (Cat# AAA12169)

Full Name

RAT ANTI MOUSE F4/80

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot

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