Application Data
(Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM.)
Her2/ERBB2 Active Protein | ERBB2 active protein
Her2/ERBB2 Protein, Human, Recombinant (ECD), Biotinylated
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Purity
>95% as determined by SDS-PAGE>95 % as determined by SEC-HPLC.
Synonyms
Her2/ERBB2; N/A; Her2/ERBB2 Protein, Human, Recombinant (ECD), Biotinylated; CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; MLN19; NEU;NGL; TKR1; ERBB2 active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
>95 % as determined by SEC-HPLC.
>95 % as determined by SEC-HPLC.
Form/Format
Lyophilized from sterile PBS, pH7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
Sequence
Met1-Thr652
Source
Human
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Thr 23
Protein Construction
A DNA sequence encoding the human ERBB2 (NP_004439.2)(Met1-Thr652) was expressed with five amino acids (DDDDK) at the C-terminus. The purified protein was biotinylated in vitro.
Reconstitution
It is recommended that the sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
Biological Activity
1. Immobilized Anti-Erbb2 Antibody at 2 ug/ml (100 ul/well) can bind Recombinant Human HER2 / ErbB2 Protein (ECD), Biotinylated (Cat: MBS8119936), the EC50 is 0.8-2.6 ng/mL.
2. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated (Cat: MBS8119936) bound to Pertuzumab was 0.1 nM.
2. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated (Cat: MBS8119936) bound to Pertuzumab was 0.1 nM.
Preparation and Storage
Samples are stable for twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Application Data
(Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM.)
Application Data
(Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM.)
SDS-PAGE
SDS-PAGE
Related Product Information for ERBB2 active protein
Background: Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Product Categories/Family for ERBB2 active protein
References
Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
The recombinant human ERBB2 consists of 636 amino acids and predicts a molecular mass of 70 kDa. As a result of glycosylation, human ErbB2 migrates as an approximately 96.7 kDa protein in SDS-PAGE under reducing conditions.
NCBI Official Full Name
receptor tyrosine-protein kinase erbB-2 isoform a
NCBI Official Synonym Full Names
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol
ERBB2
NCBI Official Synonym Symbols
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
NCBI Protein Information
receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; human epidermal growth factor receptor 2; neuro/glioblastoma derived oncogene homolog;
UniProt Protein Name
Receptor tyrosine-protein kinase erbB-2
UniProt Gene Name
ERBB2
UniProt Synonym Gene Names
HER2; MLN19; NEU; NGL; MLN 19
UniProt Entry Name
ERBB2_HUMAN
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Product Notes
The ERBB2 erbb2 (Catalog #AAA257810) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Thr65 2. It is sometimes possible for the material contained within the vial of "Her2/ERBB2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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