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WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ALB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ALB transfected lysate using ALB monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALB rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (ALB monoclonal antibody Western Blot analysis of ALB expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (90.75kD).)
Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~ 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5ug/ml])
WB (Western Blot) (PHB monoclonal antibody. Western Blot analysis of PHB expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (55.66kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
IF (Immunofluorescence) (Immunofluorescence analysis of CRC cells using LYZ antibody.)
IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using LYZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human spleen using LYZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using LYZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using LYZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using LYZ antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LYZ antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)
IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)
WB (Western Blot) (Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: C6 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat liver tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Rat brain tissue lysate, Lane 7:Rat stomach tissue lysate, Lane 8: Rat testis tissue lysate, Lane 9: Rat thymus tissue lysate, Lane 10: Rat skeletal muscle tissue lysate)
WB (Western Blot) (Western blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. lane 1: mouse heart tissue lysate, lane 2: mouse liver tissue lysate, lane 3: mouse uterus tissue lysate, lane 4: mouse lung tissue lysate, lane 5: mouse kidney tissue lysate, lane 6: mouse brain tissue lysate, lane 7: mouse stomach tissue lysate)
WB (Western Blot) (Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: A431 cell lysate,Lane 2: Caco-2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: K562 cell lysate, Lane 5: HeLa cell lysate, Lane 6: PC-3 cell lysate, Lane 7: Ramos cell lysate, Lane 8: TF-1 cell lysate, Lane 9: U20-S cell lysate, Lane 10: HL-60 cell lysate, Lane 11: HT-29 cell lysate, Lane 12: Raji cell lysate, Lane 13: HepG2 cell lysate)
WB (Western Blot) (Western blot analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ORM1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ORM1 transfected lysate using ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody. Lane 1: ORM1 transfected lysate (23.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ORM1 monoclonal antibody Western Blot analysis of ORM1 expression in MCF-7.)
WB (Western Blot) (Western Blot detection against Immunogen (45.98kD).)
Application Data (Detection limit for recombinant GST tagged PCNA is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PCNA transfected lysate using PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody Lane 1: PCNA transfected lysate (28.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCNA monoclonal antibody Western Blot analysis of PCNA expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (54.45kD).)
WB (Western Blot) (DCUN1D1 monoclonal antibody, Western Blot analysis of DCUN1D1 expression in HepG2.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCUN1D1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of DCUN1D1 expression in transfected 293T cell line by DCUN1D1 monoclonal antibody. Lane 1: DCUN1D1 transfected lysate (30.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in NIH/3T3.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
DCUN1D1 (DCN1-like Protein 1, DCUN1 Domain-containing Protein 1, Defective in Cullin Neddylation Protein 1-like Protein 1, Squamous Cell Carcinoma-related Oncogene, DCUN1L1, RP42, SCCRO) (PE)
Gene Names
DCUN1D1; RP42; SCRO; Tes3; DCNL1; SCCRO; DCUN1L1
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining ERG in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ERG in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ERG antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERG antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERG antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ERG on Jurkat cell lysates using anti-ERG antibody at 1/1, 000 dilution.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.)
IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Recombinant beta actin Monoclonal Antibody at dilution of 1:1000)
WB (Western Blot) (Western blot with Recombinant beta actin Monoclonal Antibody at dilution of 1: 20000. lane 1: C6 cell lysate, lane 2: PC-12 cell lysate, lane 3: rat spleen tissue lysate, lane 4: rat kidney tissue lysate, lane 5: rat lung tissue lysate, lane 6: rat pancreas tissue lysate, lane 7: rat brain tissue lysate, lane 8: rat stomach tissue lysate, lane 9: rat testis tissue lysate, lane 10: rat thymus tissue lysate, lane 11: rat breast tissue lysate)
WB (Western Blot) (Western blot with Recombinant beta actin Monoclonal Antibody at dilution of 1: 20000. lane 1: Raw264.7 cell lysate, lane 2: C2C12 cell lysate, lane 3: mouse lung tissue lysate, lane 4: mouse pancreas tissue lysate, lane 5: mouse brain tissue lysate, lane 6: mouse breast tissue lysate)
WB (Western Blot) (Western blot with Recombinant beta actin Monoclonal Antibody at dilution of 1: 20000. Lane 1.A431 cell lysate, Lane 2.Caco-2 cell lysate, Lane 3.Jurkat cell lysate, Lane 4.K562 cell lysate, Lane 5.HeLa cell lysate, Lane 6.PC-3 cell lysate, Lane 7.Ramos cell lysate, Lane 8.TF-1 cell lysate, Lane 9.U20-S cell lysate, Lane 10.HL-60 cell lysate, Lane 11.HT-29 cell lysate, Lane 12.Raji cell lysate, Lane 13.HepG2 cell lysate)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast tissue using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and Integrin alpha 2 (ITGA2/CD49b) knockout (KO) 293T cells, using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of various lysates, using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot) (Western blot analysis of lysates from A549 cells, using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb (AAA28479) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
ICC (Immunocytochemistry) (ICC staining ERK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ERK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ERK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: K562)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC)
Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3): endotoxin low)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Medium power)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 750)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3):RPE)
Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX54 is 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody. Lane 1: DDX54 transfected lysate (98.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
Application Data (Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in different cell lines.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Daudi Whole Cell Lysate Lane C: MOLT-4 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: 293T Whole Cell Lysate Lane F: HelaS3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lane D: Hela Whole Cell Lysate Lane E: RAW264.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Mouse heart tissue lysate Lane B: Mouse liver tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse lung tissue lysate Lane E: Mouse kidney tissue lysate Lane F: Mouse brain tissue lysate Lane G: Mouse muscle tissue lysate Lane H: Mouse stomach tissue lysate Lane I: Mouse pancreas tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:5000 dilution Lane A: Rat heart tissue lysate Lane B: Rat liver tissue lysate Lane C: Rat spleen tissue lysate Lane D: Rat lung tissue lysate Lane E: Rat kidney tissue lysate Lane F: Rat muscle tissue lysate Lane G: Rat stomach tissue lysate Lane H: Rat small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:2000 dilution Lane A: Rabbit heart tissue lysate Lane B: Rabbit liver tissue lysate Lane C: Rabbit spleen tissue lysate Lane D: Rabbit lung tissue lysate Lane E: Rabbit kidney tissue lysate Lane F: Rabbit brain tissue lysate Lane G: Rabbit muscle tissue lysate Lane H: Rabbit stomach tissue lysate Lane I: Rabbit small intestine tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
WB (Western Blot) (Anti-Beta-Tubulin mouse monoclonal antibody at 1:10000, 1:20000, 1:50000, 1:100000 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: Raw246.7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:54 kDa)
IF (Immunofluorescence) (Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.)
IHC (Immunohistochemistry) (Immunochemical staining of human Tubulin in human prostate with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry) (Immunochemical staining of human Tubulin in human malignant melanoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry) (Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from Rat lung, using MUC1 Rabbit mAb (AAA28481) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1min.)
WB (Western Blot) (Western blot analysis of lysates from Rat lung, using MUC1 Rabbit mAb (AAA28481) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with gamma Catenin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining gamma Catenin in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining gamma Catenin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining gamma Catenin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-gamma Catenin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of gamma Catenin on Hela cells lysates using anti-gamma Catenin antibody at 1/1, 000 dilution.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma Antibody was diluted at 1:200(4 degree overnight).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Phospho-eIF-2a (S51) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Phospho-eIF-2a (S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-eIF-2a (S51) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-eIF-2a (S51) on different lysates using anti-Phospho-eIF-2a (S51) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: PC12)
WB (Western Blot) (Detection of human beta-Tubulin by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Hep-G2, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of mouse beta-Tubulin by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human beta-Tubulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879 lot 1) used for IP at 20 ul/mg lysate. beta-Tubulin was also immunoprecipitated by a second antibody against a different epitope of beta-Tubulin (BL-2134A-6D11). For blotting immunoprecipitated beta-Tubulin, AAA23879 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistchemistry) (Detection of human beta-Tubulin by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of mouse beta-Tubulin by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human beta-Tubulin by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of mouse beta-Tubulin by immunocytochemistry. Sample: FFPE section of ND7/23 cells. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human beta-Tubulin (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse beta-Tubulin (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-beta-Tubulin recombinant monoclonal antibody (AAA23879) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Application Data (Proximity Ligation Analysis of protein-protein interactions between PRKCZ and AKT3. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-AKT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged AKT3 is ~0.03ng/ml as a capture antibody.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in MCF-7.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in Raw 264.7.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in PC-12.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Detection limit for recombinant GST tagged MEIS2 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEIS2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody. Lane 1: MEIS2 transfected lysate (51.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in K-562.)
WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)
Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co
WB (Western Blot) (SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (SMAD1 monoclonal antibody (M03), clone 2E9 Western Blot analysis of SMAD1 expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged TP53 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (TP53 monoclonal antibody (M01), clone 2C3 Western Blot analysis of TP53 expression in A-431 (Cat # L015V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TP53 on A-431 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TP53 on A-431 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])
WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)
WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)
WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded rat brain tissue using NeuN Rabbit mAb (AAA28482, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded mouse brain tissue using NeuN Rabbit mAb (AAA28482, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded human brain tissue using NeuN Rabbit mAb (AAA28482, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NeuN Rabbit mAb (AAA28482) at a dilution of 1?2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NeuN Rabbit mAb (AAA28482) at a dilution of 1?2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using NeuN Rabbit mAb (AAA28482) at a dilution of 1?2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using NeuN Rabbit mAb (AAA28482) at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot) (Western blot analysis of GSC over-expressed 293 cell line, cotransfected with GSC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSC monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GSC is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of GSC expression in transfected 293T cell line by GSC monoclonal antibody. Lane 1: GSC transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GSC monoclonal antibody. Western Blot analysis of GSC expression in SJCRH30.)
WB (Western Blot) (GSC monoclonal antibody. Western Blot analysis of GSC expression in COLO 320 HSR.)
WB (Western Blot) (Western Blot detection against Immunogen (37.88kD).)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)
WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with SOD1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SOD1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SOD1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SOD1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SOD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SOD1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SOD1 on MCF-7 cells lysates using anti-SOD1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot of anti-CD44 mAb against HeLa (1) and HUVE-12(2) cell lysate.)
WB (Western Blot) (Western blot of anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).)
WB (Western Blot) (Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
FCM (Flow Cytometry) (Flow cytometry of HeLa cells using anti-CD44 mAb (right) and negative control (left).)
ICC (Immunocytochemistry) (Confocal immunofluorescence of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
IF (Immunofluorescence) (Confocal immunofluorescence of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)
IHC (Immunohistochemistry) (Anti-CD44 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Purity
Ascites
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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