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WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in Raw 264.7.)
WB (Western Blot) (CMAS monoclonal antibody, Western Blot analysis of CMAS expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged CMAS is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml].)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)
Application Data (Detection limit for recombinant GST tagged PAK1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody. Lane 1: PAK1 transfected lysate (Predicted MW: 49.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PAK1 monoclonal antibody Western Blot analysis of PAK1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
ICC (Immunocytochemistry) (ICC staining STAT2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining STAT2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining STAT2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STAT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-STAT2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-STAT2 antibody. Counter stained with hematoxylin.)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with BANF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining BANF1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BANF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-BANF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-BANF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-BANF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-BANF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded cerebellum tissues using ZEB1 mouse mAb with DAB staining.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue)
WB (Western Blot) (Western blot analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate.)
WB (Western Blot) (Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA)
WB (Western Blot) (Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa))
IP (Immunoprecipitation) (Immunoprecipitation analysis of 600 ug extracts of 293T cells using 3 ug [KD Validated] CDX2 Rabbit mAb (AAA28469). Western blot was performed from the immunoprecipitate using CDX2 antibody (AAA28469) at a dilution of 1:500.)
ICC (Immunocytochemistry) (Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit mAb (AAA28469,dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KD Validated] CDX2 Rabbit mAb (AAA28469) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human appendix using [KD Validated] CDX2 Rabbit mAb (AAA28469) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (AAA28469) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot) (Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit mAb (AAA28469) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MEK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining MEK1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MEK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-MEK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MEK1 on different lysates using anti-MEK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: HepG2 Lane 3: Hela)
WB (Western Blot) (Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody. Lane 1: NFIC transfected lysate (47.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (72.82kD).)
WB (Western Blot) (Western Blot detection against Immunogen (84.7kD).)
WB (Western Blot) (Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LCK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LCK transfected lysate using LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody. Lane 1: LCK transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (LCK monoclonal antibody Western Blot analysis of LCK expression in Jurkat.)
WB (Western Blot) (Western blot analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA2 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FOXA2 transfected lysate using FOXA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FOXA2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody Lane 1: FOXA2 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA2 monoclonal antibody Western Blot analysis of FOXA2 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (36.19kD).)
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody. Lane 1: GMNN transfected lysate (23.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Smad4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Smad4 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining Smad4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Smad4 on PC-12 cells lysates using anti-Smad4 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining LYVE1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining LYVE1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining LYVE1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-LYVE1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of LYVE1 on MCF-7 lysates using anti-LYVE1 antibody at 1/1, 000 dilution.)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)
Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)
WB (Western Blot) (Detection of human CEACAM5 by western blot. Samples: Whole cell lysate (50 ug) from KM12 (10 ug), MCF-7, HEK293T, TT (2 ug), and HT-29 cells. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody (AAA23864 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human CEACAM5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from TT cells. Antibodies: Rabbit anti-CEACAM5 recombinant monoclonal antibody (AAA23864 lot 1) used for IP at 20 ul/mg lysate. CEACAM5 was also immunoprecipitated by a second antibody against a different epitope of CEACAM5 (BL-2040-1E7). For blotting immunoprecipitated CEACAM5, AAA23864 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry) (Detection of human CEACAM5 by immunohistochemistry. Sample: FFPE sections of liver carcinoma. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody (AAA23864 lot 1) (left - negative) and Rabbit anti-CEACAM1 recombinant monoclonal antibody (right - positive). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human CEACAM5 by immunohistochemistry. Sample: FFPE section of bladder carcinoma. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody (AAA23864 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human CEACAM5 by immunocytochemistry. Sample: FFPE section of over-expressing CEACAM1, CEACAM3, CEACAM5 and CEACAM6 cells. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody (AAA23864 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human CEACAM5 by immunocytochemistry. Sample: FFPE section of KM12 (colon carcinoma) cells. Antibody: Rabbit anti-CEACAM5 recombinant monoclonal antibody (AAA23864 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in NIH/3T3.)
WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged ACP1 is ~3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody. Lane 1: ACP1 transfected lysate (18kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in Raw 264.7.)
WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (43.12kD).)
ICC (Immunocytochemistry) (ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2, 000 dilution. Positive control: Lane 1: Mouse cerebellum Lane 2: Rat brain)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M04), clone 5H4.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MMP13 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MMP13 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MMP13 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MMP13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MMP13 on MCF-7 cells lysates using anti-MMP13 antibody at 1/1, 000 dilution.)
WB (Western Blot) (ACATE2 monoclonal antibody Western Blot analysis of ACATE2 expression in MCF-7.)
Application Data (Detection limit for recombinant GST tagged ACOT9 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody. Lane 1: ACOT9 transfected lysate (24kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (49.43kD).)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562 (Cat # L009V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
WB (Western Blot) (LZTFL1 monoclonal antibody, Western Blot analysis of LZTFL1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoDetection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.clonal antibody to LZTFL1 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LZTFL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of LZTFL1 expression in transfected 293T cell line by LZTFL1 monoclonal antibody. Lane 1: LZTFL1 transfected lysate (34.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (STIP1 monoclonal antibody (M11), clone 1E3 Western Blot analysis of STIP1 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with Syntenin antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Syntenin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Syntenin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntenin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syntenin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Syntenin on A549 cell using anti-Syntenin antibody at 1/2, 000 dilution.)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
Application Data (Detection limit for recombinant GST tagged STIP1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of STIP1 transfected lysate using STIP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with STIP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3ug/ml].)
WB (Western Blot) (Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody Lane 1: STIP1 transfected lysate (62.6kD). Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml])
WB (Western Blot) (SP1 monoclonal antibody (M03), clone 4C8 Western Blot analysis of SP1 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Phospho-Erk1 (T202)+Erk2 (T185) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody)
ICC (Immunocytochemistry) (ICC staining Phospho-Erk1 (T202)+Erk2 (T185) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-Erk1 (T202)+Erk2 (T185) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-Erk1 (T202)+Erk2 (T185) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Phospho-Erk1 (T202)+Erk2 (T185) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Detection of mouse P-Selectin/CD62P by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, bEnd.3 (10 ug), CH27, Spleen, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (AAA23880 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (AAA23880 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IF (Immunofluorescence) (Detection of human P-Selectin/CD62P (orange) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (AAA23880) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor Plus 647 1:400. Counterstain: DAPI (blue).)
ICC (Immunocytochemistry) (Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (AAA23880 lot 1) used for IP at 6 ul/mg lysate. P-Selectin/CD62P was also immunoprecipitated by a second antibody against a different epitope of P-Selectin/CD62P (Antibody 2). For blotting immunoprecipitated P-Selectin/CD62P, AAA23880 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
FCM (Flow Cytometry) (Detection of mouse P-Selectin/CD62P (shaded) in b.END3 cells by flow cytometry. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (AAA23880) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human P-Selectin/CD62P (shaded) in Hel92.1.7 cells by flow cytometry. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (AAA23880) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF)
Purity
Purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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