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WB (Western Blot) (Detection of human GATA3 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Hep-G2, Jurkat, RKO, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot) (Detection of mouse GATA3 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation) (Detection of human GATA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 lot 1) used for IP at 6 ul per reaction. GATA3 was also immunoprecipitated by rabbit anti-GATA3 antibody BL1707B-1C7. For blotting immunoprecipitated GATA3, AAA23839 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human GATA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 lot 1) used for IP at 6 ul per reaction. GATA3 was also immunoprecipitated by rabbit anti-GATA3 antibody BL1707B-1C7. For blotting immunoprecipitated GATA3, AAA23839 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human GATA3 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 Lot1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human GATA3 in FFPE MCF-7 cells by immunocytochemistry. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human GATA3 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-GATA3 recombinant monoclonal antibody (AAA23839 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for recombinant GST tagged S100A6 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A6 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to S100A6 on formalin-fixed paraffin-embedded human stomach carcinoma. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of S100A6 expression in transfected 293T cell line by S100A6 monoclonal antibody. Lane 1: S100A6 transfected lysate (10.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (S100A6 monoclonal antibody. Western Blot analysis of S100A6 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged MEOX2 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEOX2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml])
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in Hela NE.)
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in PC-12.)
WB (Western Blot) (MEOX2 monoclonal antibody Western Blot analysis of MEOX2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (59.07kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Integrin beta-1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta-1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Integrin beta-1 on human liver lysates using anti-Integrin beta-1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining ARID1A in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ARID1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ARID1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ARID1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ARID1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ARID1A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ARID1A antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])
WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)
WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml])
WB (Western Blot) (MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (68.53kD).)
WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
WB (Western Blot) (C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged C22orf18 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CENPM transfected lysate using CENPM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPM rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody. Lane 1: CENPM transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (45.91kD).)
CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2) (PE)
Gene Names
CENPM; PANE1; CENP-M; C22orf18
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
ICC (Immunocytochemistry) (ICC staining KMT6/EZH2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining KMT6/EZH2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining KMT6/EZH2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of KMT6/EZH2 on different lysates using anti-KMT6/EZH2 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: 293)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with IRAK2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining IRAK2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRAK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of IRAK2 on different cell lysates using anti-IRAK2 at 1/500 dilution. Positive control�� Line 1: PC-3M Line 2: A549 Line 3: HepG2)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of DLX5 expression in transfected 293T cell line by DLX5 monoclonal antibody (M12). Lane 1: DLX5 transfected lysate (31.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DLX5 monoclonal antibody, Western Blot analysis of DLX5 expression in A-431.)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in Raw 264.7.)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (35.79kD).)
WB (Western Blot) (Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody Lane 1: VRK2 transfected lysate (58.1kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged VRK2 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (VRK2 monoclonal antibody, Western Blot analysis of VRK2 expression in K-562.)
WB (Western Blot) (VRK2 monoclonal antibody. Western Blot analysis of VRK2 expression in U-2 OS.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MCM2 (2B5) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MCM2 mouse mAb with DAB staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells using MCM2 mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of MCM2 (2B5) in Hela cells using MCM2 antibody(green),and DAPI (blue).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of MCM2 (2B5) in Hela lysates using MCM2 antibody.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using MCM2 mouse mAb.)
WB (Western Blot) (Western blot analysis using MCM2 mouse mAb against MCF-7 (1), Hela (2), Jurkat (3), K562 (4), HEK293 (5) and HEPG2 (6) cell lysate.)
WB (Western Blot) (Western blot analysis of MCM2 in Jurkat, K562, 3T3 and Hela lysates using MCM2 antibody.)
WB (Western Blot) (Detection of human CD38 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, HEK293T, Jurkat, MOLT-4, RPMI-8226, CCRF-CEM, A-549, Ramos, Raji, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human CD38 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 lot 1) used for IP at 20 ul/mg lysate. CD38 was also immunoprecipitated by rabbit anti-CD38 antibody BL1874B-2A3. For blotting immunoprecipitated CD38, AAA23840 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry) (Detection of human CD38 in FFPE colon carcinoma by immunohistochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD38 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD38 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD38 in FFPE CCRF-CEM cells by immunocytochemistry. Antibody: Rabbit anti-CD38 recombinant monoclonal antibody (AAA23840 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CD38 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-CD38 recombinant monoclonal (AAA23840) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in PC-12.)
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1 Western Blot analysis of CDC2 expression in Hela S3 NE.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Peroxiredoxin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Peroxiredoxin 2 on different lysates using anti-Peroxiredoxin 2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2)
WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)
WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)
WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)
WB (Western Blot) (CCNH monoclonal antibody, Western Blot analysis of CCNH expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged CCNH is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody. Lane 1: CCNH transfected lysate (37.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
Application Data (Detection limit for recombinant GST tagged CLIC1 is 1 ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLIC1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLIC1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (CLIC1 monoclonal antibody (M01), clone 2D4 Western Blot analysis of CLIC1 expression in HL-60 (Cat # L014V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CLIC1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts of HT-29 cells using 3 ug Galectin 3/LGALS3 antibody (AAA28450). Western blot was performed from the immunoprecipitate using Galectin 3/LGALS3 antibody (AAA28450) at a dilution of 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Galectin 3/LGALS3 Rabbit mAb (AAA28450) at 1?1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
ICC (Immunocytochemistry) (ICC staining Histone H3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Histone H3 on different lysates using anti-Histone H3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: NIH/3T3)
WB (Western Blot) (Detection of mouse STAT5a by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot) (Detection of human STAT5a by western blot. Samples: Whole cell lysate from HeLa, HEK293T, C5/MJ, Hep-G2, CCRF-CEM, MJ, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human STAT5a by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) used for IP at 20 ul/mg lysate. STAT5a was also immunoprecipitated by rabbit anti-STAT5a antibody BL-1730A-2D8. For blotting immunoprecipitated STAT5a, AAA23841 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry) (Detection of mouse STAT5a in FFPE spleen by immunohistochemistry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human STAT5a in FFPE testicular seminoma by immunohistochemistry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human STAT5a in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human STAT5a in FFPE CCRF-CEM cells by immunocytochemsitry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human STAT5a (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-STAT5a recombinant monoclonal antibody (AAA23841 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for recombinant GST tagged WWP1 is 0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of WWP1 transfected lysate using WWP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with WWP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody Lane 1: WWP1 transfected lysate (105.2kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in PC-12.)
WB (Western Blot) (MGC21874 monoclonal antibody, Western Blot analysis of MGC21874 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged MGC21874 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MGC21874 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in NIH/3T3.)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co
Application Data (Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.)
Application Data (Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.)
WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in human kidney.)
WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in MCF-7.)
Application Data (PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (Detection of human SMAD2 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, K-562, A-549, RKO, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot) (Detection of mouse SMAD2 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, F9, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation) (Detection of human SMAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1) used for IP at 12 ul per reaction. SMAD2 was also immunoprecipitated by rabbit anti-SMAD2 antibody BL1658B-3F10. For blotting immunoprecipitated SMAD2, AAA23843 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry) (Detection of human SMAD2 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human SMAD2 in FFPE A549 cells by immunocytochemistry. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human Smad2 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-Smad2 recombinant monoclonal antibody (AAA23843 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)
WB (Western Blot) (EHD3 monoclonal antibody, Western Blot analysis of EHD3 expression in IMR-32.)
Application Data (Detection limit for recombinant GST tagged EHD3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EHD3 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in MCF-7.)
WB (Western Blot) (Western Blot detection against Immunogen (31.61kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate (66.4 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat (Cat # L017V1).)
Application Data (Detection limit for recombinant GST tagged NDUFA9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDUFA9 on NIH/3T3 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFA9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.8ug/ml])
WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in NIH/3T3.)
WB (Western Blot) (NDUFA9 monoclonal antibody. Western Blot analysis of NDUFA9 expression in Raw 264.7.)
WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (33.99kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (EXOSC3 monoclonal antibody, Western Blot analysis of EXOSC3 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged EXOSC3 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of EXOSC3 transfected lysate using EXOSC3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with EXOSC3 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EXOSC3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of EXOSC3 expression in transfected 293T cell line by EXOSC3 monoclonal antibody. Lane 1: EXOSC3 transfected lysate (29.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (56.36kD).)
IF (Immunofluorescence) (Immunofluorescence to CDKN1B on HeLa cell using 124808 (10ug/ml).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and CDKN1B HeLa cells were stained with AKT1 rabbit purified polyclonal (1:1200) and 124808 (1:50). Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
IHC (Immunohistochemistry) (Immunoperoxidase to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue using 124808 (5ug/ml).)
WB (Western Blot) (Western Blot analysis of CDKN1B expression in transfected 293T cell line by 124808 Lane 1: CDKN1B transfected lysate (22.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (47.89kD).)
Application Data (Detection limit for 124808 is 10ng/ml as a capture antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining eIF4EBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: 293)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Annexin A2 Rabbit mAb (AAA28452) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
Purity
Affinity purification
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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