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Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:RPE)
Application Data (Published customer image: Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.From: Estrela-Lima et al. BMC Cancer 2010 10:256.)
Application Data (Published customer image:Survival rates of animals with canine mammary carcinoma. Kaplan-Meier survival curves for animals for All (MC-BMT and MC) animals categorized according to the relative percentage of CD4+ T-cells (33.3%) (A). Animals were submitted to quarterly follow-ups during twelve months and survival rates expressed in days between the surgical excisions of the end of follow-up as described in Methods. The survival curves were estimated with the Kaplan-Meier method followed by Log-rank test. Correlation analysis highlighted the significant association between the percentages of CD4+ T-cells and CD8+ T-cells with the animal survival in days (B).From: Estrela-Lima et al. BMC Cancer 2010 10:256.)
Application Data (Published customer image: CD11b+CD14-MHCII- cells suppress T cell proliferation. Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012) Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer. PLoS ONE 7(3): e33274.)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Dog CD8)
Application Data (Staining of canine peripheral blood lymphocytes with Rat anti Canine CD8:FITC)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)
Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)
Application Data (Detection limit for recombinant GST tagged CAMKK2 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CAMKK2 transfected lysate using CAMKK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CAMKK2 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of CAMKK2 expression in transfected 293T cell line by CAMKK2 monoclonal antibody Lane 1: CAMKK2 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMKK2 monoclonal antibody Western Blot analysis of CAMKK2 expression in K-562.)
WB (Western Blot) (CAMKK2 monoclonal antibody Western Blot analysis of CAMKK2 expression in IMR-32)
WB (Western Blot) (Western Blot detection against Immunogen (40.3kD).)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in NIH/3T3.)
WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in Jurkat.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (Detection of human BMI1 by western blot. Samples: Whole cell lysate (10 ug) from MOLT-4, HeLa, HEK293T, Jurkat, LNCaP, RKO, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (10 ug) from mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1) used for IP at 6 ul/mg lysate. BMI1 was also immunoprecipitated by rabbit anti-BMI1 antibodies and BL1683A-4F6. For blotting immunoprecipitated BMI1, AAA23838 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human BMI1 in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of mouse BMI1 in FFPE renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human BMI1 in FFPE LNCaP cells by immunocytochemistry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of mouse BMI1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human BMI1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-BMI1 recombinant monoclonal antibody (AAA23838) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])
WB (Western Blot) (Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (87.56kD).)
Application Data (Detection limit for recombinant GST tagged WWP1 is 0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of WWP1 transfected lysate using WWP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with WWP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to WWP1 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to WWP1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of WWP1 expression in transfected 293T cell line by WWP1 monoclonal antibody Lane 1: WWP1 transfected lysate (105.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
ICC (Immunocytochemistry) (ICC staining CD9 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD9 on different lysates using anti-CD9 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Jurkat)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in PC-12)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in Raw 264.7)
Application Data (Detection limit for recombinant GST tagged PGRMC2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGRMC2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml])
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in NIH/3T3)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged GTF2H1 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody. Lane 1: GTF2H1 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Jurkat.)
WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (86.02kD).)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat (Cat # L017V1).)
FCM (Flow Cytometry) (Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
FCM (Flow Cytometry) (Figure-5: Epitope binding study by flow cytometric analysis. BT-474 cells expressing HER2 antigen were treated with Either Herceptin or Samceptin (1 & 2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
SDS-PAGE (Figure-4: Reducing SDS-PGE of three batches of Samceptin in comparison with Herceptin. Lanes were overloaded to show the presence of any other protein bands than Samceptin. Both heavy and light chains are well separated. (Lane-1: Marker, Lane-2: BR-13 Bulk-1 ul, Lane-3: BR-15 Bulk-1 ul, Lane-4: BR-16 Bulk-1 ul, Lane-5: Herceptin-20 ug))
Application Data
Application Data (Figure-2: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in MCF-7 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Application Data (Figure-1: Antiproliferative activity of Samceptin (Three different Batches) was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Application Data (Detection limit for recombinant GST tagged PGGT1B is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml])
WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in PC-12.)
WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.29kD).)
PGGT1B (Geranylgeranyl Transferase Type-1 Subunit beta, Geranylgeranyl Transferase Type I Subunit beta, GGTase-I-beta, Type I Protein Geranyl-Geranyltransferase Subunit beta) APC
Reactivity
Human, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody. Lane 1: MECP2 transfected lysate (52.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in NIH/3T3.)
WB (Western Blot) (MECP2 monoclonal antibody Western Blot analysis of MECP2 expression in MCF-7,)
WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in rat muscle.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Cytokeratin 19 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cytokeratin 19 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates using anti- Cytokeratin 19 antibody at 1/10, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of HL-60 cells with GEF H1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining GEF H1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GEF H1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GEF H1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GEF H1 on 293T and A431 cells lysates using anti-GEF H1 antibody at 1/500 dilution.)
WB (Western Blot) (MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MKRN2 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody. Lane 1: MKRN2 transfected lysate (46.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in human pancreas.)
Application Data (Detection limit for recombinant GST tagged CCT7 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of CCT7 expression in transfected 293T cell line by CCT7 monoclonal antibody. Lane 1: CCT7 transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in K-562.)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in Raw 264.7)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in HL-60)
WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.Lane 1: FTL transfected lysate(20 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3. GAPDH (36.1 kDa) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged SHMT1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody. Lane 1: SHMT1 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SHMT1 monoclonal antibody, Western Blot analysis of SHMT1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in PC-12.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1. Western Blot analysis of AKT2 expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (AKT2 monoclonal antibody (M06), clone X1 Western Blot analysis of AKT2 expression in Jurkat (Cat # L017V1).)
ICC (Immunocytochemistry) (ICC staining beta Tubulin in PC12 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining beta Tubulin in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining beta Tubulin in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining beta Tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of beta Tubulin on different cell lysates using anti-beta Tubulin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: PC12)
WB (Western Blot) (PKNOX2 monoclonal antibody. Western Blot analysis of PKNOX2 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody. Lane 1: PKNOX2 transfected lysate (51.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PKNOX2 monoclonal antibody, Western Blot analysis of PKNOX2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)
ICC (Immunocytochemistry) (ICC staining MEK1/2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEK1/2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MEK1/2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MEK1/2 on hybrid fish (crucian-carp) brain tissue lysates using anti-MEK1/2 antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of MEK1/2 on different cell lysates using anti-MEK1/2 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: PC12 Lane 3: NIH/3T3)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PIST in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PIST in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PIST in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PIST antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PIST antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PIST on SiHa cell using anti-PIST antibody at 1/1, 000 dilution.)
WB (Western Blot) (Detection of mouse CD3E by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human CD3E by western blot. Samples: Whole cell lysate (10 ug) from K-562, Jurkat, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1) used for IP at 12 ul/mg lysate. CD3E was also immunoprecipitated by a second antibody against a different epitope of CD3E . For blotting immunoprecipitated CD3E, AAA23851 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry) (Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of mouse CD3E in FFPE mouse spleen by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of mouse CD3E in FFPE mouse CTLL-2 cells by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD3E in FFPE MOLT-4 cells by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CD3 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23851 lo t1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (ICC staining Scavenging Receptor SR-BI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Scavenging Receptor SR-BI in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Scavenging Receptor SR-BI antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Scavenging Receptor SR-BI on different lysates using anti-Scavenging Receptor SR-BI antibody at 1/1, 000 dilution. Positive control: Lane 1: Human liver Lane 2: Mouse liver)
WB (Western Blot) (Detection of mouse SMAD4 by western blot. Samples: Whole cell lysate (30 ug) from NIH 3T3, CT26, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of human SMAD4 by western blot. Samples: Whole cell lysate (1 ug) from HEK293T, GaMG, A-549, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human SMAD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used for IP at 6 ul per reaction. SMAD4 was also immunoprecipitated by rabbit anti-SMAD4 antibody BL-1681B-1A10. For blotting immunoprecipitated SMAD4, AAA23844 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry) (Detection of human SMAD4 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human SMAD4 in FFPE RPMI-8226 cells by immunocytochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human SMAD4 in FFPE Jurkat cells by immunocytochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human Smad4 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Smad4 recombinant monoclonal (AAA23844 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue))
Application Data (Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.)
WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (75.68kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MLH1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml])
WB (Western Blot) (MLH1 monoclonal antibody Western Blot analysis of MLH1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (108.9kD).)
WB (Western Blot) (EIF2S1 monoclonal antibody, Western Blot analysis of EIF2S1 expression in HeLa.)
WB (Western Blot) (EIF2S1 monoclonal antibody. Western Blot analysis of EIF2S1 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged EIF2S1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody. Lane 1: EIF2S1 transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (60.39kD).)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in Raw 264.7.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10 Western Blot analysis of FGR expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.)
WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BST2 MaxPab rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining CDC45 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CDC45 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CDC45 antibody. Counter stained with hematoxylin.)
SHC (SHC-transforming Protein 1, SH2 Domain Protein C1, SHC1, SHC-transforming Protein 3, SHC-transforming Protein A, SHCA, Src Homology 2 Domain-containing-transforming Protein C1, FLJ26504) (AP)
Gene Names
SHC1; SHC; SHCA
Reactivity
Human
Applications
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of TIMP2 expression in HeLa.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using HLA-DQA1 Rabbit mAb (AAA28454) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HLA-DQA1 Rabbit mAb (AAA28454) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using HLA-DQA1 Rabbit mAb (AAA28454) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DQA1 Rabbit mAb (AAA28454) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using HLA-DQA1 Rabbit mAb (AAA28454) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DQA1 Rabbit mAb (AAA28454) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using HLA-DQA1 Rabbit mAb (AAA28454) at 1?1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1, 000 dilution.)
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Purity
ProA affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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