Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody. Lane 1: NQO1 transfected lysate (30.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of NQO1 expression in HepG2.)
WB (Western Blot) (Western blot analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NQO1 is ~0.3ng/ml as a capture antibody)
IP (Immunoprecipitation) (Immunoprecipitation of NQO1 transfected lysate using NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot detection against Immunogen (55.88kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (CPSF6 monoclonal antibody Western Blot analysis of CPSF6 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged CPSF6 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CPSF6 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of CPSF6 expression in transfected 293T cell line by CPSF6 monoclonal antibody. Lane 1: CPSF6 transfected lysate (59.21kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CPSF6 monoclonal antibody Western Blot analysis of CPSF6 expression in Raw 264.7.)
WB (Western Blot) (CPSF6 monoclonal antibody. Western Blot analysis of CPSF6 expression in HL-60.)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with CAMKIV antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining CAMKIV in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CAMKIV in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CAMKIV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CAMKIV antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CAMKIV on mouse brain and rat brain lysates using anti-CAMKIV antibody at 1/1, 000 dilution.)
ELISA (Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.Green: LAG3 Antibody [6D1]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.Green: LAG3 Antibody [6D1]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)
MARCH7 (E3 Ubiquitin-protein Ligase MARCH7, Axotrophin, Membrane-associated RING Finger Protein 7, Membrane-associated RING-CH Protein VII, MARCH-VII, RING Finger Protein 177, AXOT, RNF177, DKFZp586F1122)
Reactivity
Human, Mouse, Rat
Applications
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Purity
Affinity Purified Purified by Protein A affinity chromatography.
WB (Western Blot) (Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NMI is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody. Lane 1: NMI transfected lysate (35.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NMI monoclonal antibody Western Blot analysis of NMI expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)
WB (Western Blot) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)
ELISA (Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.Green: B7-H3 Antibody [7B3]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.Green: B7-H3 Antibody [7B3]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (RBM9 monoclonal antibody Western Blot analysis of RBM9 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RBM9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RBM9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibody Lane 1: RBM9 transfected lysate (40.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FUSIP1 monoclonal antibody Western Blot analysis of FUSIP1 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged FUSIP1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FUSIP1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FUSIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of FUSIP1 expression in transfected 293T cell line by FUSIP1 monoclonal antibody Lane 1: FUSIP1 transfected lysate (22.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot detection against Immunogen (36.41kD).)
Application Data (Detection limit for recombinant GST tagged TSC22D3 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of TSC22D3 transfected lysate using TSC22D3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TSC22D3 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody. Lane 1: TSC22D3 transfected lysate (22.2kD). Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of HL-60 cells with Arp3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Arp3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Arp3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Arp3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Arp3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Arp3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution. Positive control: Lane 1: A431 Lane 2: Mouse placenta Lane 3: Mouse thymus)
ELISA (A sandwich ELISA was performed using the anti-LAG3 mAbs as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.)
ELISA (Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.Green: LAG3 Antibody [5F11]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.Green: LAG3 Antibody [5F11]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
WB (Western Blot) (Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.)
WB (Western Blot) (Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IP (Immunoprecipitation) (Immunoprecipitation of ALPPL2 transfected lysate using ALPPL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALPPL2 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody Lane 1: ALPPL2 transfected lysate (57.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ALPPL2 monoclonal antibody Western Blot analysis of ALPPL2 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562 (Cat # L009V1).)
WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)
WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010 . Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)
ELISA (Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.Green: B7-H3 Antibody [2H5]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
WB (Western Blot) (Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.)
Application Data (Lamin A+C Monoclonal Antibody [JOL2])
IHC (Immunohistchemistry) (IHC using Lamin A/C antibody (IQ332) clone JOL2 on Human colon)
IB (Immunoblot) (Immunoblotting of Hela cells with Lamin A+C Antibody (IQ332) clone JOL2)
IHC (Immunohistochemistry) (IHC using Lamin A/C Antibody (IQ332) clone JOL2 on Human skin)
Application Data ((1:1000) staining in A431(A), (1:10000) staining in HeLa(B), and (1:1000) staining in HepG2(C) cells nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)
IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor 488 secondary antibody.)
WB (Western Blot) (Detection of mouse EpCAM by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, CTLL-2, EL4, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot) (Detection of human EpCAM by western blot. Samples: Whole cell lysate (50 ug) from HeLa, MCF-7, K-562, LNCaP, RKO, Hep-G2, Jurkat, HT-29, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPL23 .)
IP (Immunoprecipitation) (Detection of human EpCAM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822 lot 1) used at 12 ul per reaction. For blotting immunoprecipitated EpCAM, AAA23822 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistchemistry) (Detection of human EpCAM in FFPE colon carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry) (Detection of human EpCAM by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822-1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human EpCAM by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822-1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human EpCAM by immunocytochemistry. Sample: FFPE section of OVCAR-3 cells. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822-1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human EpCAM by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822-1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human EpCAM (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-EpCAM recombinant monoclonal antibody (AAA23822) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western Blot detection against Immunogen (60.94kD).)
IP (Immunoprecipitation) (Immunoprecipitation of ANXA5 transfected lysate using ANXA5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ANXA5 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged ANXA5 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody. Lane 1: ANXA5 transfected lysate (35.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ANXA5 monoclonal antibody, Western Blot analysis of ANXA5 expression in Hela.)
WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)
WB (Western Blot) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western Blot detection against Immunogen (41.62kD).)
Application Data (Detection limit for recombinant GST tagged C1D is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to C1D on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to C1D on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of C1D expression in transfected 293T cell line by C1D monoclonal antibody Lane 1: C1D transfected lysate (16kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (C1D monoclonal antibody Western Blot analysis of C1D expression in HeLa)
WB (Western Blot) (Western Blot analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody,. Lane 1: USF2 transfected lysate (37kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in different cell lines.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1ug/ml])
Application Data (Detection limit for recombinant GST tagged USF2 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in PC-12.)
WB (Western Blot) (USF2 monoclonal antibody Western Blot analysis of USF2 expression in HeLa.)
USF2 (Upstream Stimulatory Factor 2, Class B Basic Helix-loop-helix Protein 12, bHLHb12, FOS-interacting Protein, FIP, Major Late Transcription Factor 2, Upstream Transcription Factor 2) (PE)
Gene Names
USF2; FIP; bHLHb12
Reactivity
Human, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Filamin A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Filamin A in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Filamin A in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Filamin A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Filamin A antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Filamin A on different cell lysates using anti-Filamin A antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Jurkat Lane 3: Hela Lane 4: NIH/3T3)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SOD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SOD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SOD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SOD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SOD2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SOD2 on different lysates using anti-SOD2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y Lane 3: Human heart)
Application Data (Detection limit for recombinant GST tagged RPS5 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS5 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody. Lane 1: RPS5 transfected lysate (22.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in NIH/3T3.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in Raw 264.7.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in PC-12.)
WB (Western Blot) (RPS5 monoclonal antibody, Western Blot analysis of RPS5 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody. Lane 1: GMNN transfected lysate (23.6kD). Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NDUFB8 on different lysates using anti-NDUFB8 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: A549 Lane 3: Mouse heart Lane 4: Rat spleen)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)
Application Data (Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)
Application Data (Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)
MARCH7 (E3 Ubiquitin-protein Ligase MARCH7, Axotrophin, Membrane-associated RING Finger Protein 7, Membrane-associated RING-CH Protein VII, MARCH-VII, RING Finger Protein 177, AXOT, RNF177, DKFZp586F1122) (Biotin)
Gene Names
MARCH7; AXO; AXOT; RNF177; MARCH-VII
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in HL-60.)
WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged GLMN is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GLMN transfected lysate using GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20ug/ml])
WB (Western Blot) (Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody. Lane 1: GLMN transfected lysate (68.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (91.45kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate(66.4 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat.)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Caspase-14 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining Caspase-14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Caspase-14 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Caspase-14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Caspase-14 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Caspase-14 on MCF-7 cells lysates using anti-Caspase-14 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with SULT2A1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SULT2A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SULT2A1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SULT2A1 on mouse kidney tissue lysate using anti-SULT2A1 antibody at 1/1, 000 dilution.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:RPE)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Azide Free)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Alexa Fluor 647)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562 (Cat # L009V1).)
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Purity
Purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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