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Application Data
(RNA pol II CTD monomethyl Lys7 antibody tested for specificity Biotinylated peptides containing various RNA Pol II CTD Lysine 7 modifications were captured onto wells of a Streptavidin-coated plate and then incubated with RNA Pol II CTD K7me1 antibody at 1 ug/mL. Peptide-bound antibody was detected using anti-rat IgG HRP followed by addition of peroxidase substrate and read at A405.)
Rat anti-Human RNA pol II CTD monomethyl Lys7 Monoclonal Antibody | anti-Lys7 antibody
RNA pol II CTD monomethyl Lys7 antibody (mAb)(Clone 1F5)
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein G Chromatography
Synonyms
RNA pol II CTD monomethyl Lys7, Antibody; RNA pol II CTD monomethyl Lys7 antibody (mAb)(Clone 1F5); RNA Polymerase II Carboxy Terminal Domain; anti-Lys7 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1F5
Purity/Purification
Protein G Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Lys7 antibody
WB (Western Blot), IP (Immunoprecipitation), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This antibody was raised against a peptide containing dimethyl-lysine 7 of human RNA Pol II CTD.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(RNA pol II CTD monomethyl Lys7 antibody tested for specificity Biotinylated peptides containing various RNA Pol II CTD Lysine 7 modifications were captured onto wells of a Streptavidin-coated plate and then incubated with RNA Pol II CTD K7me1 antibody at 1 ug/mL. Peptide-bound antibody was detected using anti-rat IgG HRP followed by addition of peroxidase substrate and read at A405.)
Application Data
(RNA pol II CTD monomethyl Lys7 antibody tested for specificity Biotinylated peptides containing various RNA Pol II CTD Lysine 7 modifications were captured onto wells of a Streptavidin-coated plate and then incubated with RNA Pol II CTD K7me1 antibody at 1 ug/mL. Peptide-bound antibody was detected using anti-rat IgG HRP followed by addition of peroxidase substrate and read at A405.)
IP (Immunoprecipitation)
(RNA pol II CTD monomethyl Lys7 antibody tested by Immunoprecipitation. 5 ug of antibody was used to immunoprecipitate RNA pol II CTD monomethyl Lys7 from 300 ug of K562 nuclear extract, which was then run on SDS-PAGE and probed with the same antibody at 3 ug/ml.)
IP (Immunoprecipitation)
(RNA pol II CTD monomethyl Lys7 antibody tested by Immunoprecipitation. 5 ug of antibody was used to immunoprecipitate RNA pol II CTD monomethyl Lys7 from 300 ug of K562 nuclear extract, which was then run on SDS-PAGE and probed with the same antibody at 3 ug/ml.)
WB (Western Blot)
(RNA pol II CTD monomethyl Lys7 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)
WB (Western Blot)
(RNA pol II CTD monomethyl Lys7 antibody tested by Western blot. 20 ug of K562 nuclear extract* was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD K7me1 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Raji cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10.9M sequence tags were mapped to identify RNA pol II binding sites across a region of chromosome 12.)
ChIP (Chromatin Immunoprecipitation)
(RNA pol II CTD K7me1 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from Raji cells and 10 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 10.9M sequence tags were mapped to identify RNA pol II binding sites across a region of chromosome 12.)
Related Product Information for anti-Lys7 antibody
Short Description: Our RNA pol II CTD monomethyl Lys7 antibody (mAb)(Clone 1F5) was raised in a Rat host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: Genome-wide profiling revealed high levels of K7me1/2 marks at the transcriptional start site of genes for sense and antisense transcribing RNAPII. The new K7 modifications further expand the mammalian CTD code to allow regulation of differential gene expression. In analogy, it appears likely that the various modifications of K7 residues identified in this analysis may similarly contribute to fine tuning of gene expressing by RNAPII, and that these modifications are placed in K7 residues of CTD dependent on cell signaling and epigenetic programming of cells.
Background: Genome-wide profiling revealed high levels of K7me1/2 marks at the transcriptional start site of genes for sense and antisense transcribing RNAPII. The new K7 modifications further expand the mammalian CTD code to allow regulation of differential gene expression. In analogy, it appears likely that the various modifications of K7 residues identified in this analysis may similarly contribute to fine tuning of gene expressing by RNAPII, and that these modifications are placed in K7 residues of CTD dependent on cell signaling and epigenetic programming of cells.
Product Categories/Family for anti-Lys7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,641 Da
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB1
NCBI Official Synonym Full Names
polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
NCBI Official Symbol
POLR2A
NCBI Official Synonym Symbols
RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA polymerase II subunit B1; RNA-dir
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB1
UniProt Gene Name
POLR2A
UniProt Synonym Gene Names
POLR2; RNA polymerase II subunit B1
UniProt Entry Name
RPB1_HUMAN
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Product Notes
The Lys7 polr2a (Catalog #AAA60095) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RNA pol II CTD monomethyl Lys7 antibody (mAb)(Clone 1F5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNA pol II CTD monomethyl Lys7 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Lys7 polr2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNA pol II CTD monomethyl Lys7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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