SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
PCNA recombinant protein
PCNA, 1-261aa, Human, E Coli
Gene Names
PCNA; ATLD2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
PCNA; N/A; PCNA, 1-261aa, Human, E Coli; Proliferating cell nuclear antigen; MGC8367; PCNA,; PCNA recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid in 20mM Tris-HCl buffer (pH 7.5), containing 2mM EDTA, 20% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
Sequence Length
261
Applicable Applications for PCNA recombinant protein
SDS-PAGE
Antigen Species
Human
Preparation and Storage
Aliquot and store at -20 degree C to -80 degree C.
Avoid repeated freezing and thawing cycles.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for PCNA recombinant protein
PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by uV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E Coli and purified by conventional chromatography techniques.
Product Categories/Family for PCNA recombinant protein
References
Matsumoto K., et al. (1987) EMBO J. 6 (3):637-42. ; Moldovan GL., et al. (2007). Cell. 129 (4):665-79.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28 kDa (261 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Official Synonym Symbols
ATLD2
NCBI Protein Information
proliferating cell nuclear antigen
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
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Product Notes
The PCNA pcna (Catalog #AAA48482) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S. It is sometimes possible for the material contained within the vial of "PCNA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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