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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 2850-2900 of 10000 product results
IHC (Immunohistochemistry)
(Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
COX IV, Polyclonal Antibody (Cat# AAA19173)
Full Name
Anti-COX IV Picoband Antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
WB (Western Blot)
(WB Suggested Anti-FER1L3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate.MYOF is strongly supported by BioGPS gene expression data to be expressed in MCF7)
FER1L3, Polyclonal Antibody (Cat# AAA23526)
Full Name
FER1L3 antibody - C-terminal region
Gene Names
MYOF; FER1L3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
TALL-1, Polyclonal Antibody (Cat# AAA29164)
Full Name
TALL-1 Polyclonal Antibody
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNFSF20
Reactivity
Human, Mouse
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse breast cells using PMAIP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)
PMAIP1, Polyclonal Antibody (Cat# AAA30715)
Full Name
PMAIP1 Rabbit Polyclonal Antibody
Gene Names
PMAIP1; APR; NOXA
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-DR6/TNFRSF21 antibody (AAA19292).Overlay histogram showing A549 cells stained with AAA19292 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DR6/TNFRSF21 Antibody (AAA19292, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DR6/TNFRSF21, Polyclonal Antibody (Cat# AAA19292)
Full Name
Anti-DR6/TNFRSF21 Antibody
Gene Names
TNFRSF21; DR6; CD358; BM-018
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HEL cells using anti-Annexin-4/ANXA4 antibody (AAA19529).Overlay histogram showing HEL cells stained with AAA19529 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin-4/ANXA4 Antibody (AAA19529, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Annexin-4/ANXA4, Polyclonal Antibody (Cat# AAA19529)
Full Name
Anti-Annexin-4/ANXA4 Antibody Picoband
Gene Names
ANXA4; ANX4; PIG28; ZAP36; HEL-S-274
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of RH35 cells using anti-C1orf77/FOP/CHTOP antibody (AAA19587).Overlay histogram showing RH35 cells stained with AAA19587 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C1orf77/FOP/CHTOP Antibody (AAA19587, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
C1orf77/FOP/CHTOP, Polyclonal Antibody (Cat# AAA19587)
Full Name
Anti-C1orf77/FOP/CHTOP Antibody Picoband
Gene Names
CHTOP; FOP; SRAG; SRAG-3; SRAG-5; pp7704; C1orf77; FL-SRAG
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-FOXA1 antibody Titration: 1 ug/mLSample Type: Human liver)
FOXA1, Polyclonal Antibody (Cat# AAA23415)
Full Name
FOXA1 antibody - C-terminal region
Gene Names
FOXA1; HNF3A; TCF3A
Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rat, Yeast, Zebrafish
Applications
WB, IHC
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human lung using TXN Polyclonal Antibody at dilution of 1:100 (40x lens).)
TXN, Polyclonal Antibody (Cat# AAA22183)
Full Name
TXN Polyclonal Antibody
Gene Names
TXN; TRX; TRDX; TRX1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
WB (Western Blot)
(Figure 7. Western blot analysis of PLS1 using anti-PLS1 antibody (AAA19893).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human Caco-2 whole cell lysates,Lane 5: mouse small intestine tissue lysates,Lane 6: rat stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLS1 antigen affinity purified polyclonal antibody (AAA19893) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for PLS1 at approximately 70 kDa. The expected band size for PLS1 is at 70 kDa.)
PLS1, Polyclonal Antibody (Cat# AAA19893)
Full Name
Anti-PLS1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Detection of mouse RPA32 by western blot. Samples: Whole cell lysate (10 ug) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody (AAA23770 lot 4) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
RPA32, Polyclonal Antibody (Cat# AAA23770)
Full Name
Rabbit anti-RPA32 Antibody, Affinity Purified
Gene Names
RPA2; REPA2; RPA32
Reactivity
Human, Mouse
Applications
WB, IP, IHC, FC/FACS
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using CSK Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
CSK, Polyclonal Antibody (Cat# AAA22262)
Full Name
CSK Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Figure 9. IF analysis of SARS2 using anti-SARS2 antibody (AAA19598).SARS2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-SARS2 Antibody (AAA19598) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
SARS2, Polyclonal Antibody (Cat# AAA19598)
Full Name
Anti-SARS2 Antibody Picoband
Gene Names
SARS2; SYS; SARS; SERS; SARSM; SerRS; SerRSmt; mtSerRS
Reactivity
Human
Applications
WB, IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-Clcn5 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Mouse Brain)
Clcn5, Polyclonal Antibody (Cat# AAA23438)
Full Name
Clcn5 antibody - middle region
Gene Names
Clcn5; Clc5; ClC-5; Sfc13; Clc4-1; T25545; Clcn4-1; DXImx42e; 5430408K11Rik; D930009B12Rik
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using EEF1B2 antibody. Blue: DAPI for nuclear staining.)
EEF1B2, Polyclonal Antibody (Cat# AAA28114)
Full Name
EEF1B2 Polyclonal Antibody
Gene Names
EEF1B2; EF1B; EEF1B; EEF1B1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
MDM2, Polyclonal Antibody (Cat# AAA29169)
Full Name
MDM2 Polyclonal Antibody
Gene Names
MDM2; HDMX; hdm2; ACTFS
Reactivity
Human
Applications
WB, IHC-P, IF
Pricing
WB (Western Blot)
CEACAM5, Polyclonal Antibody (Cat# AAA19192)
Full Name
Anti-CEACAM5 Antibody
Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of PLD3 using anti-PLD3 antibody (AAA19440).PLD3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-PLD3 Antibody (AAA19440) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
PLD3, Polyclonal Antibody (Cat# AAA19440)
Full Name
Anti-PLD3 Antibody Picoband
Gene Names
PLD3; AD19; HUK4; HU-K4; SCA46
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-PRDM9 Antibody Titration: 1 ug/mlPositive Control: 293T cells lysate)
PRDM9, Polyclonal Antibody (Cat# AAA23451)
Full Name
PRDM9 antibody - middle region
Gene Names
PRDM9; PFM6; KMT8B; MSBP3; ZNF899; MEISETZ
Reactivity
Human
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-NUP37 antibody (AAA19966).Overlay histogram showing MCF-7 cells stained with AAA19966 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUP37 Antibody (AAA19966, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NUP37, Polyclonal Antibody (Cat# AAA19966)
Full Name
Anti-NUP37 Antibody Picoband
Gene Names
NUP37; p37
Reactivity
Human, Rat
Applications
EIA, IF, IHC, ICC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of MCF-7 cells using anti-EHD3 antibody (AAA19295).Overlay histogram showing MCF-7 cells stained with AAA19295 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD3 Antibody (AAA19295, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EHD3, Polyclonal Antibody (Cat# AAA19295)
Full Name
Anti-EHD3 Antibody
Gene Names
EHD2; PAST2
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(EPDR/EPDR1 Antibody-Immunohistochemistry of paraffin-embedded Human liver cancer using EPDR1 Polyclonal Antibody at dilution of 1:40.)
EPDR/EPDR1, Polyclonal Antibody (Cat# AAA21382)
Full Name
EPDR/EPDR1 Rabbit anti-Human Polyclonal Antibody
Reactivity
Mouse, Rat, Human
Applications
EIA, IHC-P, WB
Purity
Affinity purification
Pricing
Application Data
(Dilution: WB 1:1000-2000, IHC 1:100-200)
Ghrelin Receptor, Polyclonal Antibody (Cat# AAA28900)
Full Name
Ghrelin Receptor Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CISD2 using anti-CISD2 antibody (AAA19311).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey kidney tissue lysatesLane 5: human SW620 whole cell lysatesLane 6: human Raji whole cell lysatesLane 7: rat kidney tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CISD2 antigen affinity purified polyclonal antibody (Catalog # AAA19311) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CISD2 at approximately 15KD. The expected band size for CISD2 is at 15KD.)
CISD2, Polyclonal Antibody (Cat# AAA19311)
Full Name
Anti-CISD2 Antibody
Gene Names
CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U87 cells using anti-GNG2 antibody (AAA19312).Overlay histogram showing U87 cells stained with AAA19312 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG2 Antibody (AAA19312, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GNG2, Polyclonal Antibody (Cat# AAA19312)
Full Name
Anti-GNG2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-GJA4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateGJA4 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
GJA4, Polyclonal Antibody (Cat# AAA23457)
Full Name
GJA4 antibody - middle region
Gene Names
GJA4; CX37
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IP (Immunoprecipitation)
(Figure 6. Immunoprecipitating RPL27 in Hela whole cell lysate .Western blot analysis of RPL27 using anti-RPL27 antibody (AAA19932).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-RPL27 antibody in Hela whole cell lysate.Lane 3: anti-RPL27 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (AAA19932) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL27 Antibody (AAA19932, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPL27, Polyclonal Antibody (Cat# AAA19932)
Full Name
Anti-RPL27 Antibody Picoband
Gene Names
RPL27; L27
Reactivity
Human, Mouse, Rat
Applications
WB, IP, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A431 cells using anti-AKAP 95/AKAP8 antibody (AAA19538).Overlay histogram showing A431 cells stained with AAA19538 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKAP 95/AKAP8 Antibody (AAA19538, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AKAP 95/AKAP8, Polyclonal Antibody (Cat# AAA19538)
Full Name
Anti-AKAP 95/AKAP8 Antibody Picoband
Gene Names
AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HepG2 cells using anti-HSPA1L antibody (AAA19810).Overlay histogram showing HepG2 cells stained with AAA19810 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA1L Antibody (AAA19810, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HSPA1L, Polyclonal Antibody (Cat# AAA19810)
Full Name
Anti-HSPA1L Antibody Picoband
Gene Names
HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-TGFB1I1 Antibody Titration: 0.2-1 ug/mlPositive Control: Human Lung)
TGFB1I1, Polyclonal Antibody (Cat# AAA23425)
Full Name
TGFB1I1 Antibody - middle region
Gene Names
TGFB1I1; HIC5; ARA55; HIC-5; TSC-5
Reactivity
Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Mouse brain using BRSK1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
BRSK1, Polyclonal Antibody (Cat# AAA22210)
Full Name
BRSK1 Polyclonal Antibody
Gene Names
BRSK1; hSAD1
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity purification
Pricing
WB (Western Blot)
(Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (10 ug) from U2OS, HEK293T, Hep-G2, HeLa, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM9 antibody (AAA23776 lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
FOX2/RBM9, Polyclonal Antibody (Cat# AAA23776)
Full Name
Rabbit anti-FOX2/RBM9 Antibody, Affinity Purified
Gene Names
RBFOX2; RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3
Reactivity
Human, Mouse, Rat
Applications
WB, IP, IHC, FC/FACS
Purity
Antigen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using PIN1 antibody. Blue: DAPI for nuclear staining.)
PIN1, Polyclonal Antibody (Cat# AAA29684)
Full Name
PIN1 Antibody
Gene Names
PIN1; DOD; UBL5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
METTL1, Polyclonal Antibody (Cat# AAA19336)
Full Name
Anti-METTL1 Antibody
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse DGKz AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Diacylglycerol Kinase Zeta, Polyclonal Antibody (Cat# AAA20882)
Full Name
Polyclonal Antibody to Diacylglycerol Kinase Zeta (DGKz)
Gene Names
Dgkz; mDGK[z]; E130307B02Rik; F730209L11Rik
Reactivity
Mouse, Human, Rat
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of HepG2 cells using anti-PELI3 antibody (AAA19930).Overlay histogram showing HepG2 cells stained with AAA19930 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PELI3 Antibody (AAA19930, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PELI3, Polyclonal Antibody (Cat# AAA19930)
Full Name
Anti-PELI3 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using ASPSCR1 antibody. Blue: DAPI for nuclear staining.)
ASPSCR1, Polyclonal Antibody (Cat# AAA28119)
Full Name
ASPSCR1
Gene Names
ASPSCR1; TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEL cells using anti-STXBP6 antibody (AAA19956).Overlay histogram showing HEL cells stained with AAA19956 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP6 Antibody (AAA19956, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
STXBP6, Polyclonal Antibody (Cat# AAA19956)
Full Name
Anti-STXBP6 Antibody Picoband
Gene Names
STXBP6; amisyn; HSPC156
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-ACTR1B Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateACTR1B is supported by BioGPS gene expression data to be expressed in HepG2)
ACTR1B, Polyclonal Antibody (Cat# AAA23545)
Full Name
ACTR1B antibody - C-terminal region
Gene Names
ACTR1B; PC3; ARP1B; CTRN2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CRP, Polyclonal Antibody (Cat# AAA29174)
Full Name
CRP Polyclonal Antibody
Gene Names
CRP; PTX1
Reactivity
Human, Rat
Applications
WB, IHC-P
Pricing
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 10-20% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 15 kDa isoform is identified, and a second isoform of 18 kDa is also present in some samples.)
PFN1, Polyclonal Antibody (Cat# AAA23532)
Full Name
PFN1 antibody - N-terminal region
Gene Names
PFN1; ALS18
Reactivity
Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
Predicted Species Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(WB Suggested Anti-GRSF1 Antibody Titration: 0.2-1 ug/mlPositive Control: Hela cell lysateGRSF1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)
GRSF1, Polyclonal Antibody (Cat# AAA23475)
Full Name
GRSF1 antibody - middle region
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)
Ran, Polyclonal Antibody (Cat# AAA19131)
Full Name
Anti-Ran Picoband Antibody
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of JK cells using anti-GSPT1,2 antibody (AAA19586).Overlay histogram showing JK cells stained with AAA19586 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSPT1,2 Antibody (AAA19586, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GSPT1,2, Polyclonal Antibody (Cat# AAA19586)
Full Name
Anti-GSPT1,2 Antibody Picoband
Gene Names
GSPT1; GST1; ETF3A; eRF3a; 551G9.2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: MUC1Sample Type: HepG2Lane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)
MUC1, Polyclonal Antibody (Cat# AAA23492)
Full Name
MUC1 antibody - C-terminal region
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Pig
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using GRSF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
GRSF1, Polyclonal Antibody (Cat# AAA28367)
Full Name
GRSF1 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-SLC39A6 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysate)
SLC39A6, Polyclonal Antibody (Cat# AAA23513)
Full Name
SLC39A6 antibody - middle region
Gene Names
SLC39A6; ZIP6; LIV-1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep
Applications
IHC, WB
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of CD105 using anti-CD105 antibody (AAA19165).CD105 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD105 Antibody (AAA19165) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD105, Polyclonal Antibody (Cat# AAA19165)
Full Name
Anti-CD105 Picoband Antibody
Gene Names
Eng; Endo; CD105; AI528660; AI662476; S-endoglin
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
DB (Dot Blot)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Phospho-HIST1H3B3 (S10), Polyclonal Antibody (Cat# AAA28670)
Full Name
Phospho-HIST1H3B3 (S10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
DB, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
SHH, Polyclonal Antibody (Cat# AAA23516)
Full Name
SHH antibody - N-terminal region
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)
Applications
IHC, WB
Purity
Affinity Purified
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.