Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with HKDC1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

HKDC1, Polyclonal Antibody (Cat# AAA29946)

Full Name

HKDC1 Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, FC/FACS

Purity

Protein affinity purified

Pricing

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Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)

Full Name

Anti-Coronin 1a/TACO/CORO1A Antibody

Gene Names

CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-NEDD4 antibody (AAA19413).Overlay histogram showing U87 cells stained with AAA19413 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NEDD4 Antibody (AAA19413, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NEDD4, Polyclonal Antibody (Cat# AAA19413)

Full Name

Anti-NEDD4 Antibody Picoband

Gene Names

NEDD4; RPF1; NEDD4-1

Reactivity

Human, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-NSUN2 antibody (AAA19802).Overlay histogram showing 293T cells stained with AAA19802 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSUN2 Antibody (AAA19802, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of NSUN2 using anti-NSUN2 antibody (AAA19802).NSUN2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN2 antigen affinity purified polyclonal antibody (#AAA19802) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NSUN2 at approximately 100 kDa. The expected band size for NSUN2 is at 100 kDa.)

NSUN2, Polyclonal Antibody (Cat# AAA19802)

Full Name

Anti-NSUN2 Antibody Picoband

Gene Names

NSUN2; MISU; MRT5; SAKI; TRM4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot)

FEN1, Polyclonal Antibody (Cat# AAA19194)

Full Name

Anti-FEN1 Antibody

Gene Names

FEN1; MF1; RAD2; FEN-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-GIPC1 antibody (AAA19828).Overlay histogram showing HepG2 cells stained with AAA19828 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GIPC1 Antibody (AAA19828, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of GIPC1 using anti-GIPC1 antibody (AAA19828).GIPC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: rat skeletal muscle tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: rat NRK whole cell lysates,Lane 6: mouse skeletal muscle tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (#AAA19828) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.)

GIPC1, Polyclonal Antibody (Cat# AAA19828)

Full Name

Anti-GIPC1 Antibody Picoband

Gene Names

GIPC1; NIP; GIPC; IIP-1; TIP-2; SEMCAP; C19orf3; Hs.6454; GLUT1CBP; RGS19IP1; SYNECTIN; SYNECTIIN

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-NIT2 antibody (AAA19853).Overlay histogram showing A549 cells stained with AAA19853 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NIT2 Antibody (AAA19853, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of NIT2 using anti-NIT2 antibody (AAA19853).NIT2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NIT2 Antibody (AAA19853) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates,Lane 3: rat kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIT2 antigen affinity purified polyclonal antibody (#AAA19853) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NIT2 at approximately 33 kDa. The expected band size for NIT2 is at 33 kDa.)

NIT2, Polyclonal Antibody (Cat# AAA19853)

Full Name

Anti-NIT2 Antibody Picoband

Gene Names

NIT2; HEL-S-8a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-RBM47 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: NCI-H226)

RBM47, Polyclonal Antibody (Cat# AAA23486)

Full Name

RBM47 antibody - middle region

Gene Names

RBM47; NET18

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-ESR2 antibody Titration: 1 ug/mLSample Type: Human liver)

ESR2, Polyclonal Antibody (Cat# AAA23449)

Full Name

ESR2 antibody - N-terminal region

Gene Names

ESR2; Erb; ESRB; ODG8; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

AMD1/Adometdc, Polyclonal Antibody (Cat# AAA19177)

Full Name

Anti-AMD1/Adometdc Antibody

Gene Names

AMD1; AMD; SAMDC; ADOMETDC

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CIRBP antibody. Blue: DAPI for nuclear staining.)

CIRBP, Polyclonal Antibody (Cat# AAA28159)

Full Name

CIRBP Polyclonal Antibody

Gene Names

CIRBP; CIRP

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-TBP-1/PSMC3 antibody (AAA19314).Overlay histogram showing 293T cells stained with AAA19314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBP-1/PSMC3 Antibody (AAA19314, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TBP-1/PSMC3, Polyclonal Antibody (Cat# AAA19314)

Full Name

Anti-TBP-1/PSMC3 Antibody

Gene Names

PSMC3; TBP1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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SCML1, Polyclonal Antibody (Cat# AAA19625)

Full Name

Anti-SCML1 Antibody Picoband

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-Tropomyosin 2/TPM2 antibody (AAA19572).Overlay histogram showing SiHa cells stained with AAA19572 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (AAA19572, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Tripeptidyl peptidase II/TPPII/TPP2, Polyclonal Antibody (Cat# AAA19572)

Full Name

Anti-Tripeptidyl peptidase II/TPPII/TPP2 Antibody Picoband

Gene Names

TPP2; TPP-2; TPPII; TPP-II

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using FOLH1 Polycloanl Antibody at dilution of 1:200)

FOLH1, Polyclonal Antibody (Cat# AAA22168)

Full Name

FOLH1 Polyclonal Antibody

Gene Names

Folh1; GCP2; mopsm

Reactivity

Human, Mouse, Rat

Applications

IHC

Purity

Antigen affinity purification

Pricing

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WB (Western Blot)

Desmoglein 2/DSG2, Polyclonal Antibody (Cat# AAA19196)

Full Name

Anti-Desmoglein 2/DSG2 Antibody

Gene Names

DSG2; HDGC; CDHF5

Reactivity

Human

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-TMPRSS3 antibody (AAA19289).Overlay histogram showing A431 cells stained with AAA19289 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMPRSS3 Antibody (AAA19289, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TMPRSS3, Polyclonal Antibody (Cat# AAA19289)

Full Name

Anti-TMPRSS3 Antibody

Gene Names

TMPRSS3; DFNB8; DFNB10; ECHOS1; TADG12

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U937 cells using anti-BDH1 antibody (AAA19328).Overlay histogram showing U937 cells stained with AAA19328 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDH1 Antibody (AAA19328,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

BDH1, Polyclonal Antibody (Cat# AAA19328)

Full Name

Anti-BDH1 Antibody

Gene Names

BDH1; BDH; SDR9C1

Reactivity

Human, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (CD40 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

CD40, Polyclonal Antibody (Cat# AAA21366)

Full Name

CD40 Rabbit anti-Human Polyclonal Antibody

Gene Names

CD40; p50; Bp50; CDW40; TNFRSF5

Reactivity

Mouse, Rat, Human

Applications

IF, IHC-P, EIA, WB

Purity

Peptide affinity chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ZNF177 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ZNF177, Polyclonal Antibody (Cat# AAA22184)

Full Name

ZNF177 Polyclonal Antibody

Gene Names

ZNF177; PIGX

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using PEX14 antibody. Blue: DAPI for nuclear staining.)

PEX14, Polyclonal Antibody (Cat# AAA28157)

Full Name

PEX14

Gene Names

PEX14; NAPP2; PBD13A; Pex14p; dJ734G22.2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Pricing

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WB (Western Blot) (WB Suggested Anti-PPP3CA Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle)

PPP3CA, Polyclonal Antibody (Cat# AAA23542)

Full Name

PPP3CA antibody - middle region

Gene Names

PPP3CA; CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; ACCIID; CALNA1; IECEE1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HELA cells using anti-DNAJC10 antibody (AAA19320).Overlay histogram showing HELA cells stained with AAA19320 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJC10 Antibody (AAA19320, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

DNAJC10, Polyclonal Antibody (Cat# AAA19320)

Full Name

Anti-DNAJC10 Antibody

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-CACNB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate)

CACNB1, Polyclonal Antibody (Cat# AAA23439)

Full Name

CACNB1 antibody - N-terminal region

Gene Names

CACNB1; CAB1; CCHLB1; CACNLB1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Akt2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Akt2, Polyclonal Antibody (Cat# AAA22177)

Full Name

Akt2 Polyclonal Antibody

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-PYHIN1 antibody (AAA19944).Overlay histogram showing JK cells stained with AAA19944 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYHIN1 Antibody (AAA19944, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PYHIN1 using anti-PYHIN1 antibody (AAA19944).PYHIN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYHIN1 antigen affinity purified polyclonal antibody (#AAA19944) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PYHIN1 at approximately 55 kDa. The expected band size for PYHIN1 is at 55 kDa.)

PYHIN1, Polyclonal Antibody (Cat# AAA19944)

Full Name

Anti-PYHIN1 Antibody Picoband

Gene Names

PYHIN1; IFIX

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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Interleukin 4, Polyclonal Antibody (Cat# AAA20956)

Full Name

Polyclonal Antibody to Interleukin 4 (IL4)

Reactivity

Chicken, Human, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ITCH antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

ITCH, Polyclonal Antibody (Cat# AAA28409)

Full Name

ITCH Rabbit pAb

Gene Names

MYL6B; MLC1SA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC

Purity

Affinity purification

Pricing

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NASP, Polyclonal Antibody (Cat# AAA28154)

Full Name

NASP

Gene Names

NASP; FLB7527; PRO1999

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U87 cells using anti-SPG3A/ATL1 antibody (AAA19441).Overlay histogram showing U87 cells stained with AAA19441 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPG3A/ATL1 Antibody (AAA19441, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SPG3A/ATL1, Polyclonal Antibody (Cat# AAA19441)

Full Name

Anti-SPG3A/ATL1 Antibody Picoband

Gene Names

ATL1; FSP1; GBP3; SPG3; HSN1D; SPG3A; AD-FSP; atlastin1

Reactivity

Human

Applications

FC/FACS, IHC, WB

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-PDIA6 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: HepG2 cell lysate.PDIA6 is strongly supported by BioGPS gene expression data to be expressed in HepG2)

PDIA6, Polyclonal Antibody (Cat# AAA23546)

Full Name

PDIA6 antibody - middle region

Gene Names

PDIA6; P5; ERP5; TXNDC7

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-ADRB1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain)

ADRB1, Polyclonal Antibody (Cat# AAA23455)

Full Name

ADRB1 antibody - middle region

Gene Names

ADRB1; RHR; B1AR; ADRB1R; BETA1AR

Reactivity

Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat

Applications

Purity

Affinity Purified

Pricing

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DDX41, Polyclonal Antibody (Cat# AAA23454)

Full Name

DDX41 antibody - N-terminal region

Gene Names

DDX41; ABS; MPLPF

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Purity

Affinity Purified

Pricing

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<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-PANX1 Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)

PANX1, Polyclonal Antibody (Cat# AAA23505)

Full Name

PANX1 antibody - middle region

Gene Names

PANX1; PX1; MRS1; UNQ2529

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

BAI1, Polyclonal Antibody (Cat# AAA28895)

Full Name

BAI1 Polyclonal Antibody

Gene Names

BAI1; GDAIF

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

TSPAN12, Polyclonal Antibody (Cat# AAA19174)

Full Name

Anti-TSPAN12 Picoband antibody

Gene Names

TSPAN12; EVR5; NET2; NET-2; TM4SF12

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ITCH Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

ITCH, Polyclonal Antibody (Cat# AAA22264)

Full Name

ITCH Polyclonal Antibody

Gene Names

ITCH; AIF4; AIP4; NAPP1; dJ468O1.1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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HSP70, Polyclonal Antibody (Cat# AAA28405)

Full Name

HSP70 Rabbit pAb

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HL-60 cells using anti-SSBP1 antibody (AAA19836).Overlay histogram showing HL-60 cells stained with AAA19836 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSBP1 Antibody (AAA19836, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of SSBP1 using anti-SSBP1 antibody (AAA19836).SSBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SSBP1 Antibody (AAA19836) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: human A549 whole cell lysates,Lane 6: human A375 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSBP1 antigen affinity purified polyclonal antibody (#AAA19836) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SSBP1 at approximately 16 kDa. The expected band size for SSBP1 is at 17-18 kDa.)

SSBP1, Polyclonal Antibody (Cat# AAA19836)

Full Name

Anti-SSBP1 Antibody Picoband

Gene Names

SSBP1; SSBP; mtSSB; Mt-SSB; SOSS-B1

Reactivity

Human

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

TF, Polyclonal Antibody (Cat# AAA29172)

Full Name

TF Polyclonal Antibody

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

WB, IHC-P

Pricing

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GNG11, Polyclonal Antibody (Cat# AAA19638)

Full Name

Anti-GNG11 Antibody Picoband

Gene Names

GNG11; GNGT11

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (ATG16L1/ATG16L Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

ATG16L1/ATG16L, Polyclonal Antibody (Cat# AAA21358)

Full Name

ATG16L1/ATG16L Rabbit anti-Human Polyclonal Antibody

Gene Names

ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Immunoaffinity purified

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-PCMT1 antibody (AAA19819).Overlay histogram showing K562 cells stained with AAA19819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCMT1 Antibody (AAA19819, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PCMT1 using anti-PCMT1 antibody (AAA19819).PCMT1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (#AAA19819) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 25-28 kDa.)

PCMT1, Polyclonal Antibody (Cat# AAA19819)

Full Name

Anti-PCMT1 Antibody Picoband

Gene Names

PCMT1; PIMT

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using BTRC antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

BTRC, Polyclonal Antibody (Cat# AAA28368)

Full Name

BTRC Rabbit pAb

Gene Names

BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with Kv1.4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Kv1.4, Polyclonal Antibody (Cat# AAA29925)

Full Name

Kv1.4 Antibody

Gene Names

Kcna4; Kv1.4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, FC/FACS

Purity

Peptide affinity purified

Pricing

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<< Previous Next >>

IF (Immunofluorescence) (Figure 8. IF analysis of VPS53 using anti-VPS53 antibody (AAA19576).VPS53 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL rabbit anti-VPS53 Antibody (AAA19576) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

VPS53, Polyclonal Antibody (Cat# AAA19576)

Full Name

Anti-VPS53 Antibody Picoband

Gene Names

VPS53; HCCS1; PCH2E; hVps53L; pp13624

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Caco-2 cells using anti-BCCIP antibody (AAA19854).Overlay histogram showing Caco-2 cells stained with AAA19854 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCCIP Antibody (AAA19854, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of BCCIP using anti-BCCIP antibody (AAA19854).BCCIP was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BCCIP Antibody (AAA19854) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: human A431 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCCIP antigen affinity purified polyclonal antibody (#AAA19854) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCCIP at approximately 45 kDa. The expected band size for BCCIP is at 36 kDa.)

BCCIP, Polyclonal Antibody (Cat# AAA19854)

Full Name

Anti-BCCIP Antibody Picoband

Gene Names

BCCIP; TOK1; TOK-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using p27 KIP 1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

p27 KIP1, Polyclonal Antibody (Cat# AAA22188)

Full Name

p27 KIP1 Polyclonal Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-RPL18 antibody (AAA19881).Overlay histogram showing Hela cells stained with AAA19881 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL18 Antibody (AAA19881, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RPL18 using anti-RPL18 antibody (AAA19881).RPL18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Daudi whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL18 antigen affinity purified polyclonal antibody (#AAA19881) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPL18 at approximately 23 kDa. The expected band size for RPL18 is at 22 kDa.)

RPL18, Polyclonal Antibody (Cat# AAA19881)

Full Name

Anti-RPL18 Antibody Picoband

Gene Names

RPL18; L18

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using RPL13 antibody. Blue: DAPI for nuclear staining.)

RPL13, Polyclonal Antibody (Cat# AAA28110)

Full Name

RPL13 Polyclonal Antibody

Gene Names

RPL13; L13; BBC1; D16S44E; D16S444E

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Polyclonal Antibodies

Viewing 2900-2950 of 10000 product results

HKDC1, Polyclonal Antibody (Cat# AAA29946)

Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)

NEDD4, Polyclonal Antibody (Cat# AAA19413)

NSUN2, Polyclonal Antibody (Cat# AAA19802)

FEN1, Polyclonal Antibody (Cat# AAA19194)

GIPC1, Polyclonal Antibody (Cat# AAA19828)

NIT2, Polyclonal Antibody (Cat# AAA19853)

RBM47, Polyclonal Antibody (Cat# AAA23486)

ESR2, Polyclonal Antibody (Cat# AAA23449)

AMD1/Adometdc, Polyclonal Antibody (Cat# AAA19177)

CIRBP, Polyclonal Antibody (Cat# AAA28159)

TBP-1/PSMC3, Polyclonal Antibody (Cat# AAA19314)

SCML1, Polyclonal Antibody (Cat# AAA19625)

Tripeptidyl peptidase II/TPPII/TPP2, Polyclonal Antibody (Cat# AAA19572)

FOLH1, Polyclonal Antibody (Cat# AAA22168)

Desmoglein 2/DSG2, Polyclonal Antibody (Cat# AAA19196)

TMPRSS3, Polyclonal Antibody (Cat# AAA19289)

BDH1, Polyclonal Antibody (Cat# AAA19328)

CD40, Polyclonal Antibody (Cat# AAA21366)

ZNF177, Polyclonal Antibody (Cat# AAA22184)

PEX14, Polyclonal Antibody (Cat# AAA28157)

PPP3CA, Polyclonal Antibody (Cat# AAA23542)

DNAJC10, Polyclonal Antibody (Cat# AAA19320)

CACNB1, Polyclonal Antibody (Cat# AAA23439)

Akt2, Polyclonal Antibody (Cat# AAA22177)

PYHIN1, Polyclonal Antibody (Cat# AAA19944)

Interleukin 4, Polyclonal Antibody (Cat# AAA20956)

ITCH, Polyclonal Antibody (Cat# AAA28409)

NASP, Polyclonal Antibody (Cat# AAA28154)

SPG3A/ATL1, Polyclonal Antibody (Cat# AAA19441)

PDIA6, Polyclonal Antibody (Cat# AAA23546)

ADRB1, Polyclonal Antibody (Cat# AAA23455)

DDX41, Polyclonal Antibody (Cat# AAA23454)

PANX1, Polyclonal Antibody (Cat# AAA23505)

BAI1, Polyclonal Antibody (Cat# AAA28895)

TSPAN12, Polyclonal Antibody (Cat# AAA19174)

ITCH, Polyclonal Antibody (Cat# AAA22264)

HSP70, Polyclonal Antibody (Cat# AAA28405)

SSBP1, Polyclonal Antibody (Cat# AAA19836)

TF, Polyclonal Antibody (Cat# AAA29172)

GNG11, Polyclonal Antibody (Cat# AAA19638)

ATG16L1/ATG16L, Polyclonal Antibody (Cat# AAA21358)

PCMT1, Polyclonal Antibody (Cat# AAA19819)

BTRC, Polyclonal Antibody (Cat# AAA28368)

Kv1.4, Polyclonal Antibody (Cat# AAA29925)

VPS53, Polyclonal Antibody (Cat# AAA19576)

BCCIP, Polyclonal Antibody (Cat# AAA19854)

p27 KIP1, Polyclonal Antibody (Cat# AAA22188)

RPL18, Polyclonal Antibody (Cat# AAA19881)

RPL13, Polyclonal Antibody (Cat# AAA28110)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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