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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 2650-2700 of 10000 product results
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RAF1 (EE), Recombinant Protein (Cat# AAA73890)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
c-KIT (K642E), Recombinant Protein (Cat# AAA73296)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CAMK1, Recombinant Protein (Cat# AAA73301)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CAMK1 gamma, Recombinant Protein (Cat# AAA73305)
SDS-PAGE
(The purity of Camk2α was determined to be >85% by densitometry. Approx. MW 74kDa)
CAMK2 alpha, Recombinant Protein (Cat# AAA73306)
SDS-PAGE
(The purity was determined to be >70% by densitometry. CAMK2γ Approx. MW 60kDa.)
CAMK2 gamma, Recombinant Protein (Cat# AAA73311)
Application Data
COVID 19 Spike S1 (T19R, delta157-158, L452R, T478K, D614G, P681R) Coronavirus, Recombinant Protein (Cat# AAA73327)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
COVID 19 Spike RBD (E484Q) Coronavirus, Recombinant Protein (Cat# AAA73336)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
COVID 19 Spike RBD (N440K) Coronavirus, Recombinant Protein (Cat# AAA73338)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CK1 alpha 1, Recombinant Protein (Cat# AAA73374)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MASTL, Recombinant Protein (Cat# AAA73737)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MYLK4, Recombinant Protein (Cat# AAA73739)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TRKC (G623R), Recombinant Protein (Cat# AAA73769)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
NUAK1, Recombinant Protein (Cat# AAA73774)
SDS-PAGE
(Purity: Sample purity data. for lot-specific data please refer to the specific datasheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
FGFR4 (V550M), Recombinant Protein (Cat# AAA73560)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
FLT3 (N676K), Recombinant Protein (Cat# AAA73570)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
GSK3 alpha, Recombinant Protein (Cat# AAA73580)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EIF2AK1 (HRI), Recombinant Protein (Cat# AAA73593)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
IRAK4 (catalytic domain), Recombinant Protein (Cat# AAA73620)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
c-KIT (D820Y), Recombinant Protein (Cat# AAA73629)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
c-KIT (T670E), Recombinant Protein (Cat# AAA73631)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
c-KIT (Y823D), Recombinant Protein (Cat# AAA73632)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
ubc7 (ubiquitin Conjugating enzyme 7), Recombinant Protein (Cat# AAA48452)
Application Data
(Recombinant Polynucleosomes (H3.1) protein activity assay. Results show that polynucleosomes are better substrates than octamers for NSD1-SET and NSD2-SET. Lanes 5-9 represent polynucleosomes that have been subjected to subsequent freeze-thaw cycles to demonstrate their stability. Activity was detected by fluorography.)
Polynucleosomes, Recombinant Protein (Cat# AAA59700)
Application Data
(Recombinant KMT2B (MLL4) complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 20 nM Recombinant KMT2B (MLL4) complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: 50 turnovers/ enzyme molecule)
KMT2B, Recombinant Protein (Cat# AAA59720)
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant polynucleosomes and histone octamers as substrates. 2 ug Recombinant Polynucleosomes H3.3 (K36M) were incubated with DOT1L in reaction buffer for 3 hours at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for DOT1L than histone octamers.)
Polynucleosomes H3.3, Recombinant Protein (Cat# AAA59752)
Application Data
(HTRF assay for p53 (TP53) activity 1 uM oligo ds DNA (sequence: 5’-CCGGACATGCCCGGGCATGT-3’) was incubated with BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
p53, Recombinant Protein (Cat# AAA60128)
Application Data
(HTRF assay for recombinant AKT2 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT2 protein in a 10 ul reaction system for 1 hour. The 10 ul detection reagents were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature, and HTRF KinEASE STK assay was used to detect the enzymatic activity.)
AKT2, Recombinant Protein (Cat# AAA60155)
Application Data
(Recombinant Sortase (2A.9) protein specificity for LAETG sequence Sortase (2A.9) at 12 ng/ul was incubated with 0.4 mM LAETG, LPETG or LPESG substrates in reaction buffer, followed by fluorescence detection upon cleavage. Results demonstrate the high specificity of Sortase (2A.9) for LAETG. The 2A.9 shows minimal activity to the WT substrate LPETG and no activity towards the mutated substrate LPESG.)
Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
JARID1C/KDM5C, Recombinant Protein (Cat# AAA59681)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.1 (K4M) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie bule. * indicates streptavidin. The SDS-PAGE gel result showed that >80% of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60191)
Application Data
(HTRF for IGF2BP3 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP3 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
IGF2BP3, Recombinant Protein (Cat# AAA60211)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.1 (R26C) – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. The beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel stained with Coomassie blue. The SDS-PAGE gel shows that >88% of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60214)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (K4I) – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. The beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60218)
SDS-PAGE
Luciferase, Recombinant Protein (Cat# AAA48630)
SDS-PAGE
14-3-3 sigma, Recombinant Protein (Cat# AAA48633)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.