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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 4450-4500 of 10000 product results
Application Data
Malaria Pv. MSP antigen, Recombinant Protein (Cat# AAA78001)
Full Name
Malaria Pv. MSP antigen (merozoite surface protein)
Applications
ELISA, Rapid Test
Purity
>95% pure (l0% SDS-PAGE, Coomassie blue stain)
Pricing
Application Data
GRA8, Recombinant Protein (Cat# AAA78032)
Full Name
Toxoplasma gondii GRA8
Applications
Lateral Flow, Western Blot
Purity
>95% pure (12% SDS-PAGE, Coomassie blue stain). Proprietary chromatographic technique
Pricing
proBNP, Recombinant Protein (Cat# AAA78137)
Full Name
Human recombinant proBNP
Purity
Immunoaffinity purification followed by ion exchange chromatography.
Pricing
Prolactin, antagonist (del 1-9) G129R, Recombinant Protein (Cat# AAA79334)
Full Name
Human Prolactin, antagonist (del 1-9) G129R
Purity
> 97% by SDS-PAGE & HPLC analyses
Pricing
SDS-PAGE
PPARG, Recombinant Protein (Cat# AAA178162)
Full Name
Recombinant Human PPARG protein (His tag)
Gene Names
PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
Purity
>80 % as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
COVID 19 Spike S2 (ECD) Coronavirus, Recombinant Protein (Cat# AAA177013)
Full Name
Recombinant 2019-nCoV Spike S2 Protein (ECD, Fc Tag)
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
SDS-PAGE
MOG, Recombinant Protein (Cat# AAA173582)
Full Name
Recombinant Mouse MOG (aa30-149) Protein (His tag)
Gene Names
Mog; B230317G11Rik
Purity
>97 % as determined by SDS-PAGE
Pricing
Application Data
(Bovine VEGF-A ELISARecombinant bovine VEGF-A detected using Rabbit anti Bovine VEGF-A as the capture reagent and Rabbit anti Bovine VEGF-A:Biotin as the detection reagent followed by Streptavidin:HRP)
VEGF-A, Recombinant Protein (Cat# AAA50283)
Full Name
RECOMBINANT BOVINE VEGF-A
Gene Names
VEGFA; VPF; VEGF; VEGF-A; eVEGF120; eVEGF164
Applications
ELISA
Purity
>95% by SDS PAGE analysis
Pricing
Application Data
(HTRF assay for STK11 / LKB1 activity 1 uM STK S1 substrate was incubated with different concentrations of STK11 / LKB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
STK11/LKB1, Recombinant Protein (Cat# AAA60249)
Full Name
Recombinant STK11/LKB1 protein
Gene Names
STK11; PJS; LKB1; hLKB1
Pricing
Application Data
(HTRF assay for MAP3K8 / COT (30-397) activity 1 uM STK S3 substrate was incubated with different concentrations of MAP3K8 / COT (30-397) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10u?l detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
MAP3K8/COT, Recombinant Protein (Cat# AAA60250)
Full Name
Recombinant MAP3K8 (30-397)/COT protein
Gene Names
MAP3K8; COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT
Pricing
Application Data
(HTRF assay for MBD2-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD2-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for MBD4-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD4-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(AMP-Glo assay for UBE2A activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2A in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2A, Recombinant Protein (Cat# AAA60379)
Full Name
Recombinant UBE2A protein
Purity
>=95% by SDS-PAGE.
Pricing
Application Data
(AMP-Glo assay for UBE2E1 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2E1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2E1, Recombinant Protein (Cat# AAA60381)
Full Name
Recombinant UBE2E1 protein
Purity
>=95% by SDS-PAGE.
Pricing
Application Data
(HTRF assay for Recombinant NTRK1 (440-796, F589L) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, F589L) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
NTRK1, Recombinant Protein (Cat# AAA60384)
Full Name
Recombinant NTRK1 (440-796, F589L) protein
Pricing
Application Data
(HTRF assay for NTRK1 (440-796, A608D) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, A608D) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
NTRK1, Recombinant Protein (Cat# AAA60386)
Full Name
Recombinant NTRK1 (440-796, A608D) protein
Pricing
Application Data
(AMP-Glo assay for UBE2R2 activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2R2 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2R2, Recombinant Protein (Cat# AAA60396)
Full Name
Recombinant UBE2R2 protein
Purity
>=85% by SDS-PAGE.
Pricing
Application Data
(AMP-Glo assay for UBA1 activity 7.9uM ubiquitin and 25uM ATP were incubated with different concentrations of UBA1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBA1, Recombinant Protein (Cat# AAA60404)
Full Name
Recombinant UBA1 protein
Purity
>=95% by SDS-PAGE.
Pricing
Application Data
(HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
BRD2, Recombinant Protein (Cat# AAA60412)
Full Name
Recombinant BRD2 protein
Purity
>=95% by SDS-PAGE.
Pricing
Application Data
(HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
BRD4, Recombinant Protein (Cat# AAA60413)
Full Name
Recombinant BRD4 protein
Purity
>=95% by SDS-PAGE.
Pricing
Application Data
(ADP-Glo assay for CDK9/CyclinT1 Complex activity 0.2 ug/ul PDKtide peptide substrate was incubated with different concentrations of CDK9/CyclinT1 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
CDK9/CyclinT1, Recombinant Protein (Cat# AAA60416)
Full Name
Recombinant CDK9/CyclinT1 protein complex
Pricing
Application Data
(ADP-Glo assay for PI3Ka/p85 Complex activity 0.125 ug/ul PI-3 Kinase Lipid Substrate was incubated with different concentrations of PI3Ka/p85 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
PI3Kalpha/p85, Recombinant Protein (Cat# AAA60419)
Full Name
Recombinant PI3Kalpha/p85 protein complex
Pricing
Application Data
(HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
PLK3, Recombinant Protein (Cat# AAA60243)
Full Name
Recombinant PLK3 (57-340) protein
Gene Names
PLK3; CNK; FNK; PRK
Pricing
Application Data
(HTRF assay for JAK3 (781-1124) activity 1 uM TK substrate was incubated with different concentrations of JAK3 (781-1124) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JAK3, Recombinant Protein (Cat# AAA60244)
Full Name
Recombinant JAK3 (781-1124) protein
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Pricing
Growth differentiation factor 11, Recombinant Protein (Cat# AAA59114)
Full Name
Growth differentiation factor 11, GDF11
Gene Names
GDF11; BMP11; BMP-11
Pricing
SDS-PAGE
Cytokeratin Fragment Antigen 21-1, Recombinant Protein (Cat# AAA55765)
Full Name
Recombinant Human Cytokeratin Fragment Antigen 21-1 (CYFRA21-1) Protein
Purity
> 90 % as determined by SDS-PAGE.
Pricing
Bioactivity
(Detects Human IL15 Antibody in indirect ELISAs.)
IL15, Recombinant Protein (Cat# AAA120479)
Full Name
Recombinant Human IL15, C-His
Gene Names
IL15; IL-15
Applications
Western Blot
Purity
>95% as determined by SDS-PAGE
Pricing
WB (Western Blot)
(Recombinant Mononucleosomes H3K27ac - biotin tested by Western blot Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes (H3K27ac) - biotinylated (Lane 2) were detected with anti-H3K27ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes (H3K27ac) - biotinylated can be detected by anti-H3K27ac antibody.)
Mononucleosomes H3K27ac, Recombinant Protein (Cat# AAA60099)
Full Name
Recombinant Mononucleosomes H3K27ac-biotin
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Mononucleosomes H3.3 (K27M). 1 ug PRC2 Complex was incubated with 2 ug wild type Mononucleosomes H3.3 or K27M mutant Mononucleosomes H3.3, respectively, in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western blot was used to detect the generation of reaction products (Anti-H3K27me3). The Western blot result shows that K27M mutation inhibits the activity of PRC2 Complex.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60101)
Full Name
Recombinant Mononucleosomes H3.3 (K27M)-biotin
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Purity
The recombinant protein is >=95% by SDS-PAGE gel.
Pricing
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant nucleosomes and histone octamers as substrates. 2 ug biotinylated mononucleosomes H3.3 (G34V) were incubated with 0.2 ug DOT1L (1-420) in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (Anit-H3K79me1). DOT1L only was used as negative control.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60103)
Full Name
Recombinant Mononucleosomes H3.3 (G34V)-biotin
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Purity
The recombinant protein is >=95% pure by SDS-PAGE gel analysis.
Pricing
WB (Western Blot)
(Recombinant SUV420H2 (2-281) activity assay Recombinant Nucleosomes (H3.1) were incubated with SUV420H2 (2-281) protein. Reaction products were run on SDS-PAGE, and detected by Western Blot using Anti-H4K20me3.Results show the activity of SUV420H2 (2-281) with nucleosomes as substrate is better than with histone octamers.)
SUV420H2, Recombinant Protein (Cat# AAA60106)
Full Name
Recombinant SUV420H2 (2-281) protein
Gene Names
SUV420H2; KMT5C
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Mononucleosomes H3K9ac (EPL) - biotin. Recombinant Mononucleosomes H3K9ac(EPL) - biotinylated (Lane 1) and unmodified nucleosomes (Lane 2) were detected with anti-H3K9ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K9ac (EPL) - biotin can be detected by anti-H3K9ac antibody.)
Mononucleosomes H3K9ac, Recombinant Protein (Cat# AAA60108)
Full Name
Recombinant Mononucleosomes H3K9ac (EPL)-biotin
Gene Names
HIST1H2AC; H2A/l; H2AFL; dJ221C16.4
Purity
The recombinant protein is >=95% by SDS-PAGE gel.
Pricing
Application Data
(Recombinant IDO1 protein activity assay 400 uM L-tryptophan was incubated with different concentrations of IDO1 protein in reaction buffer mixture. The reaction was stopped by the addition of 40 ul 30% (w/v) trichloroacetic acid and heated at 65 degree C for 15 min. After cooling down to 4 degree C, the reaction was centrifuged at 1,125 g for 10 min. 100 ul supernatant was transferred into 96-well plate and mixed with 100 ul of 2% (w/v) p-DMAB. The yellow pigment derived from kynurenine was measured at 490 nm.)
IDO1, Recombinant Protein (Cat# AAA60111)
Full Name
Recombinant IDO1 protein
Gene Names
IDO1; IDO; INDO; IDO-1
Pricing
Application Data
(HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)
NFkB1 p50, Recombinant Protein (Cat# AAA60112)
Full Name
Recombinant NFkB1 p50 (1-434) protein
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Pricing
Application Data
(Recombinant Mononucleosomes (H2A.Z) DNA gel Mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes)
Mononucleosomes, Recombinant Protein (Cat# AAA60120)
Full Name
Recombinant Mononucleosomes (H2A.Z)
Gene Names
H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
Pricing
Application Data
(HTRF for YTHDC1 (325-502) ActivityOligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YTHDC1, Recombinant Protein (Cat# AAA60133)
Full Name
Recombinant YTHDC1 (325-502) protein
Gene Names
YTHDC1; YT521; YT521-B
Pricing
Application Data
(ALKBH2 dioxygenase activity assay 1 ug of ssDNA oligos (5’-AAAGCAG(1mA)ATTCGAAAAAGCGAAA-3’) was incubated with 0 ug, 0.5 ug, 1 ug, 2 ug, 4 ug of ALKBH2 in 50 ul reaction system including 50 mM HEPES-NaOH pH 8.0, 50 ?M Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. 1/4 reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)
ALKBH2, Recombinant Protein (Cat# AAA60148)
Full Name
Recombinant ALKBH2 protein
Gene Names
ALKBH2; ABH2
Pricing
Application Data
(Recombinant NgTet1 activity assay 1 ug of 30 nt ssDNA oligos with a 5mCpG site were incubated with different concentrations of NgTet1 in a reaction system at 34 degree C for 1 hour. Sample was concentrated to 10 ul, and 1 ul was spotted onto nylon membrane. 5hmC Ab and 5-caC Ab were used to detect products. NgTet1 activity assay was detected by Dot-Blot..)
Application Data
(HTRF assay for BRD4 (44-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (44-460), GST-tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
BRD4, Recombinant Protein (Cat# AAA60164)
Full Name
Recombinant BRD4 (44-460) protein, GST-Tag
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Pricing
WB (Western Blot)
(Recombinant p300 protein activity assay 0.5 ug of Histone H4 were incubated with 0 (-), 12.5 (+), 25 (++), or 50 (+++) ng of p300 protein, respectively in a 20 ul reaction system including 50 mM Tris pH 8.0, 1 mM TCEP, 0.1 mM EDTA, 50 ng/ul BSA and 20 uM acetyl-CoA for 30 minutes at 30 degree C. For each reaction, 7.5 ul product was run on a 12.5% SDS-PAGE gel and detected by Western Blot. p300 only was used as negative control. H4 pan-acetyl antibody was used to recognize products.)
p300, Recombinant Protein (Cat# AAA60167)
Full Name
Recombinant p300 protein
Gene Names
EP300; p300; KAT3B; RSTS2
Pricing
Application Data
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
MLLT3/AF9, Recombinant Protein (Cat# AAA60171)
Full Name
Recombinant MLLT3/AF9 (1-138) protein, His/DYKDDDDK Tag
Gene Names
MLLT3; AF9; YEATS3
Pricing
Application Data
(Recombinant YEATS4 / GAS41 protein activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS4 / GAS41 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YEATS4, Recombinant Protein (Cat# AAA60172)
Full Name
Recombinant YEATS4 (GAS41) protein
Gene Names
YEATS4; YAF9; GAS41; NUBI-1; 4930573H17Rik; B230215M10Rik
Pricing
Application Data
(HTRF assay for EGFR (672-1210, L858R) protein activity 1 micro;M TK substrate was incubated with different concentrations of EGFR (672-1210, L858R) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE TK assay was used to detect the enzymatic activity.)
EGFR, Recombinant Protein (Cat# AAA60175)
Full Name
Recombinant EGFR protein (672-1210, L858R) protein
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Pricing
Application Data
(HTRF assay for LATS1 protein activity 1 uM STK S1 substrate was incubated with different concentrations of LATS1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hr. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.HTRF KinEASE STK assay was used to detect the enzymatic activity.)
LATS1, Recombinant Protein (Cat# AAA60177)
Full Name
Recombinant LATS1 protein
Gene Names
LATS1; wts; WARTS
Pricing
SDS-PAGE
(DNMT1 protein gel. DNMT1 (2.8 ug) run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue. Arrow indicates recombinant DNMT1.)
Application Data
(Recombinant CREBBP (1081-1197) HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of CREBBP (1081-1197) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in reaction buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
CREBBP, Recombinant Protein (Cat# AAA59643)
Full Name
Recombinant CREBBP (1081-1197) protein
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for PRC2 Complex activity 1 uM H3 (16-37) peptide was incubated with different concentrations of PRC2 Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours, then 10 ul of H3K27me1 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at RT. HTRF assay was used for detection.)
PRC2, Recombinant Protein (Cat# AAA59652)
Full Name
Recombinant PRC2 complex
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Pricing
Application Data
(HTRF Assay for Recombinant SMARCA4/BRG1 (1448-1569) Activity. 3.3 uM histone peptide H3K14ac was incubated with SMARCA4/BRG1 (1448-1569) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SMARCA4/BRG1, Recombinant Protein (Cat# AAA59660)
Full Name
Recombinant SMARCA4/BRG1 (1448-1569) protein
Gene Names
SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A
Pricing
WB (Western Blot)
(Western blot for PRMT1 methyltransferase activity 0.5 ug Histone H4 was incubated with 0 ug (-), 0.05 ?g (+), 0.1 ug (++), 0.2 ug (+++) PRMT1 system for 2 hr at room temperature. Products were detected by Western blot using H4R3me2a (asymmetric) antibody.)
Application Data
(Recombinant DNMT3B protein activity assay Recombinant DNMT3B protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
DNMT3B, Recombinant Protein (Cat# AAA59665)
Full Name
Recombinant DNMT3B protein
Gene Names
DNMT3B; ICF; ICF1; M.HsaIIIB
Purity
The recombinant protein is >60% pure by SDS-PAGE.
Pricing
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.