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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 4600-4650 of 14163 product results
SDS-PAGE
Fucosyltransferase 4 (FUT4), Recombinant Protein (Cat# AAA135583)
SDS-PAGE
Thyroglobulin (TG), Recombinant Protein (Cat# AAA135492)
SDS-PAGE
(Figure. SDS-PAGE)
Complement Receptor 2 (CR2), Recombinant Protein (Cat# AAA135511)
SDS-PAGE
Caspase 11 (CASP11), Recombinant Protein (Cat# AAA135518)
Application Data
(HTRF assay for ERBB2 (676-1255) activity 1uM TK substrate was incubated with different concentrations of ERBB2 (676-1255) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 1 mM MnCl2, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for Recombinant MECP2 (80-166) protein activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MECP2 (80-166) protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for PDGFRA (550-1089, D842V) activity 1uM TK substrate was incubated with different concentrations of PDGFRA (550-1089, D842V) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl, 1 mM DTT, 5 nM SEB and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
PDGFRA, Recombinant Protein (Cat# AAA60387)
Application Data
(AMP-Glo assay for UBE2E3 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2E3 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2E3, Recombinant Protein (Cat# AAA60399)
ELISA
(ELISA for XRCC1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of XRCC1 were added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
XRCC1, Recombinant Protein (Cat# AAA60405)
Application Data
(HTRF assay for CAMK2D activity 1uM STK S1 substrate was incubated with different concentrations of CAMK2D protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 500uM CaCl2,1uM Calmodulin, 1 mM DTT, 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
TSHR 420, Recombinant Protein (Cat# AAA59128)
HCV core, Recombinant Protein (Cat# AAA71909)
West Nile Virus Envelope, Recombinant Protein (Cat# AAA71882)
Application Data
gag gene, Recombinant Protein (Cat# AAA72852)
Application Data
(This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.573)
Phl p 5a, Recombinant Protein (Cat# AAA72865)
SDS-PAGE
COL6A3 / Collagen-VI, Recombinant Protein (Cat# AAA173518)
Bioactivity
(Human CCL2/MCP-1 chemoattracts the the THP-1 human acute monocytic leukemia cells. The ED50 range )
CCL2/JE/MCP-1, Recombinant Protein (Cat# AAA48416)
SDS-PAGE
Synaptobrevin 1, Recombinant Protein (Cat# AAA48437)
SDS-PAGE
HtrA2/Omi, Recombinant Protein (Cat# AAA48468)
SDS-PAGE
TALDO1, Recombinant Protein (Cat# AAA48619)
SDS-PAGE
MAGEA4, Recombinant Protein (Cat# AAA48620)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
TDO2, Recombinant Protein (Cat# AAA48657)
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
alpha-Synuclein, Recombinant Protein (Cat# AAA48667)
SDS-PAGE
uFM1, Recombinant Protein (Cat# AAA48668)
SDS-PAGE
TSN, Recombinant Protein (Cat# AAA48679)
BDH2, Recombinant Protein (Cat# AAA48693)
SDS-PAGE
PLAC8, Recombinant Protein (Cat# AAA48847)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
GLuD1, Recombinant Protein (Cat# AAA48857)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualization by coomassie blue stain.)
CD8B, Recombinant Protein (Cat# AAA48869)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
HP, Recombinant Protein (Cat# AAA48876)
SDS-PAGE
NRGN, Recombinant Protein (Cat# AAA48877)
SDS-PAGE
CNPY1, Recombinant Protein (Cat# AAA48883)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
BRAF, Recombinant Protein (Cat# AAA48888)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SERPINB3, Recombinant Protein (Cat# AAA48901)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
CADM1, Recombinant Protein (Cat# AAA48903)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
IL1a, Recombinant Protein (Cat# AAA48906)
SDS-PAGE
BAIAP2, Recombinant Protein (Cat# AAA48763)
SDS-PAGE
GSTA4, Recombinant Protein (Cat# AAA48777)
SDS-PAGE
MGP, Recombinant Protein (Cat# AAA48785)
SDS-PAGE
CD226, Recombinant Protein (Cat# AAA48790)
SDS-PAGE
BGN, Recombinant Protein (Cat# AAA48793)
SDS-PAGE
TRIM28, Recombinant Protein (Cat# AAA48804)
SDS-PAGE
XPA, Recombinant Protein (Cat# AAA48809)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
NHLH1, Recombinant Protein (Cat# AAA48811)
SDS-PAGE
IGFBP7, Recombinant Protein (Cat# AAA48815)
SDS-PAGE
CPOX, Recombinant Protein (Cat# AAA48827)
SDS-PAGE
MBP, Recombinant Protein (Cat# AAA48833)
SDS-PAGE
CEACAM7, Recombinant Protein (Cat# AAA48837)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
mug, Recombinant Protein (Cat# AAA48842)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
ABRACL, Recombinant Protein (Cat# AAA48846)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.