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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 4500-4550 of 10000 product results
DB (Dot Blot)
(Dot Blot analysis for recombinant TET3 (824-1795) protein. 250 ng of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 ug, 312.5 and 625 ng TET3 protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at room temperature for 1 hour. Sample was concentrated to 5 ul, then 1 ul sample was spotted onto positively charged nylon membrane. 5-hmC antibody was used to detect the generation of products.)
Tet3, Recombinant Protein (Cat# AAA59672)
Full Name
Recombinant Tet3 (824-1795) protein
Gene Names
TET3; hCG_40738
Pricing
Application Data
(Recombinant JARID1B / KDM5B protein activity assay. 1 ?M H3K4me3 peptide was incubated with different concentrations of JARID1B / KDM5B protein in 10 ?l reaction system, then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JARID1B/KDM5B, Recombinant Protein (Cat# AAA59680)
Full Name
Recombinant JARID1B/KDM5B protein
Gene Names
KDM5B; CT31; PLU1; PUT1; PLU-1; JARID1B; FLJ10538; FLJ12459; FLJ12491; FLJ16281; FLJ23670; RBBP2H1A
Purity
The recombinant protein is >75% pure by SDS-PAGE.
Pricing
Application Data
(HTRF Assay for Recombinant BRD1 (556-688) activity. 3.3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRD1 (556-688) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
BRD1, Recombinant Protein (Cat# AAA59683)
Full Name
Recombinant BRD1 (556-688) protein
Gene Names
BRD1; BRL; BRPF1; BRPF2
Purity
The recombinant protein is >86% pure by SDS-PAGE.
Pricing
SDS-PAGE
(NSD2 (MMSET) activity assay. 2 ug recombinant nucleosomes were incubated with NSD2 in 30 ul reaction system for 3 hr. at room temp. Reaction products were run on a 12.5% SDS-PAGE gel and detected with H3K36me2 antibody. NSD2 only was used as negative control. Results show that nucleosomes are better than octamers as substrates for NSD2. )
NSD2, Recombinant Protein (Cat# AAA59692)
Full Name
Recombinant NSD2 (MMSET) protein
Purity
The recombinant protein is >= 75% pure by SDS-PAGE.
Pricing
Application Data
(JMJD2C / KDM4C activity assay.1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2C / KDM4C protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate, 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JMJD2C/KDM4C, Recombinant Protein (Cat# AAA59695)
Full Name
Recombinant JMJD2C/KDM4C protein
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for UTX / KDM6A activity 3 uM H3K27me3 peptide was incubated with different concentrations of UTX / KDM6A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul of H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
WB (Western Blot)
(Histone Methyltransferase (HMT) Assay comparing Recombinant Polynucleosomes (H3.3) and histone octamers as substrates. 2 ug Recombinant Polynucleosomes (H3.3) were incubated with 0 ug , 0.25 ug, 0.5 ug and 1 ug NSD2-SET in reaction buffer for 3 hours at room temperature, respectively. Western Blot was used to detect the generation of reaction products (H3K36me2). NSD2-SET only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for NSD2 than histone octamers.)
Polynucleosomes, Recombinant Protein (Cat# AAA59702)
Full Name
Recombinant Polynucleosomes (H3.3)
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
Application Data
(NSD1-SET activity assay using Recombinant Nucleosomes as substrates. Recombinant Nucleosomes were used as substrates in an assay measuring the methyltransferase activity of NSD1-SET. Activity was detected by fluorography.)
NSD1-SET, Recombinant Protein (Cat# AAA59705)
Full Name
Recombinant NSD1-SET protein
Gene Names
NSD1; STO; KMT3B; SOTOS; ARA267; SOTOS1
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
Application Data
(Recombinant SETD7 protein activity assay. 2 ug Calf Thymus Octamers was incubated with 0.5 ug SETD7 protein in reaction buffer containing radioactive 3H-SAM for 2 hour at room temperature. Activity was detected by fluorography.)
SETD7, Recombinant Protein (Cat# AAA59717)
Full Name
Recombinant SETD7 protein
Gene Names
SETD7; KMT7; SET7; SET9; SET7/9
Purity
The recombinant protein is >80% pure by SDS-PAGE.
Pricing
Application Data
(HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
SIRT1, Recombinant Protein (Cat# AAA59738)
Full Name
Recombinant SIRT1 (193-741) protein
Purity
Recombinant SIRT1 (193-741) protein that includes amino acids 193-741 of human SIRT1 protein (accession number NM_012238.4) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa.The purity ofThe protein is > 80% b
Pricing
Application Data
(HDAC6 (H230A) Protein activity assay. 3 uM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (H230A) protein in reaction buffer for 30 minutes at 37 degree C followed by developing for 30 minutes at room temperature. Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
HDAC6, Recombinant Protein (Cat# AAA59753)
Full Name
Recombinant HDAC6 (H230A) protein
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Pricing
WB (Western Blot)
(Western Blot analysis for Recombinant Histone H3K79me3 (MLA) Recombinant Histone H3K27me3 (MLA) (Lane 1) and Histone H3.1 (Lane 2) were detected with H3K79me2me3 antibody . Histones stained by coomassie blue below were as loading control.)
Histone H3K79me3, Recombinant Protein (Cat# AAA59760)
Full Name
Recombinant Histone H3K79me3 (MLA)
Gene Names
HIST1H3A; H3/A; H3FA
Purity
The recombinant histone is >92% pure by SDS-PAGE.
Pricing
DB (Dot Blot)
(Dot blot for Recombinant ALKBH5 protein activity. 3 uM m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with different concentrations of ALKBH5 proteins in a 30 ul reaction system including 50 mM HEPES-NaOH pH 7.5, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Then each reaction was concentrated to a final volume of 10 ul. 1 ul of each reaction was spotted onto a positively charged nylon membrane and products were detected by Dot blot assay with m6A antibody .)
ALKBH5, Recombinant Protein (Cat# AAA59767)
Full Name
Recombinant ALKBH5 protein
Gene Names
ALKBH5; ABH5; OFOXD; OFOXD1
Pricing
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K79me2 (MLA). Expected mass: 15271 Da Observed mass: 15271 Da)
Histone H3K79me2, Recombinant Protein (Cat# AAA59776)
Full Name
Recombinant Histone H3K79me2 (MLA)
Gene Names
HIST1H3A; H3/A; H3FA
Pricing
Application Data
(Recombinant IDH1 (R131C) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH1 (R132C) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
Application Data
(10 uM NADPH and 1 uM α-KG were incubated with 100 nM IDH1 (R132H) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 45 min.)
Application Data
(Recombinant IDH2 protein activity. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH2 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
IDH2, Recombinant Protein (Cat# AAA59785)
Full Name
Recombinant IDH2 protein
Gene Names
IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
Pricing
Application Data
(Recombinant IDH2 (R172K) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH2 (R172K) protein in 200 ul reaction system (50 mM Tris-HCl pH 7.4, 150 mM NaCl. 10 mM MgCl2 and 0.03% BSA, room temp). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
IDH2, Recombinant Protein (Cat# AAA59788)
Full Name
Recombinant IDH2 (R172K) protein
Gene Names
IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
Pricing
Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
PDK1, Recombinant Protein (Cat# AAA60178)
Full Name
Recombinant PDK1 protein, GST-Tag
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Pricing
WB (Western Blot)
(Recombinant KAT6B / MORF (718-1008) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6B / MORF (718-1008) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3ac (pan-acetyl) antibody .)
KAT6B/MORF, Recombinant Protein (Cat# AAA60181)
Full Name
Recombinant KAT6B/MORF (718-1008) protein
Gene Names
KAT6B; qkf; MORF; MOZ2; GTPTS; MYST4; ZC2HC6B; querkopf
Pricing
SDS-PAGE
(Recombinant Estrogen Receptor-alpha protein gel 9% SDS-PAGE gel with Coomassie blue staining MW: 67.5 kDa Purity: >80%)
Estrogen Receptor a, Recombinant Protein (Cat# AAA60203)
Full Name
Recombinant Estrogen Receptor a protein
Gene Names
ESR1; ER; ESR; Era; ESRA; NR3A1
Pricing
Application Data
(MTase-Glo Assay for Recombinant SETD6 protein methyltransferase activity 1 uM substrate peptide and 1 uM SAM were incubated with different concentrations of recombinant SETD6 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence was read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (R8C) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60207)
Full Name
Recombinant Mononucleosomes H3.3 (R8C)-biotin
Gene Names
H3F3A; H3F3; H3.3A
Pricing
Application Data
(HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
IGF2BP2, Recombinant Protein (Cat# AAA60210)
Full Name
Recombinant IGF2BP2 protein
Gene Names
IGF2BP2; IMP2; IMP-2; VICKZ2
Pricing
Application Data
(HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)
SDS-PAGE
Rantes, Recombinant Protein (Cat# AAA58763)
Full Name
Rantes
Gene Names
CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
(>95% by SDS-PAGE)
Stromal Cell-Derived Factor 1 alpha (SDF-1a) Recombinant, Recombinant Protein (Cat# AAA58299)
Full Name
SDF-1a, Recombinant
Gene Names
CXCL12; IRH; PBSF; SDF1; TLSF; SDF1A; SDF1B; TPAR1; SCYB12
Reactivity
Human
Purity
>98% pure (RP-HPLC and SDS-PAGE). Purified by chromatographic techniques
Product is sterile filtered.
Product is sterile filtered.
Plasminogen Activator Inhibitor 1, Recombinant, Recombinant Protein (Cat# AAA58301)
Full Name
Mouse PAI-1, Recombinant
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Reactivity
Mouse
Purity
>98% pure (SDS-PAGE). 80 + 5% active by uPA titration. Column chromatography
Pricing
Mycobacterium tuberculosis, 38kDa, Recombinant, Recombinant Protein (Cat# AAA58344)
Full Name
M. tuberculosis, 38kDa, Recombinant
Applications
ELISA
Purity
95% pure (SDS-PAGE)
Pricing
SDS-PAGE
Integrin-Linked Kinase (ILK), Recombinant Protein (Cat# AAA58744)
Full Name
Integrin-Linked Kinase (ILK)
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK; HEL-S-28
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
Interleukin-4 (IL-4), Recombinant Protein (Cat# AAA58753)
Full Name
Interleukin-4 (IL-4)
Purity
>95% by SDS-PAGE
Pricing
SDS-PAGE
Monoamine Oxidase B (MAOB), Recombinant Protein (Cat# AAA58755)
Full Name
Monoamine Oxidase B (MAOB)
Purity
>95% by SDS-PAGE
Pricing
Cytomegalovirus, Recombinant Protein (Cat# AAA58386)
Full Name
Cytomegalovirus (CMV) pp 52 (UL44) Recombinant
Applications
ELISA
Purity
> 90% pure (determined by SDS-PAGE). Purified by Glutathione Sepharose 4B (GS-4B).
Purified
Purified
Pricing
WB (Western Blot)
(Western blot analysis in order to detect streptavidin /biotin reaction. The incubation was performed with HRP conjugated streptavidin (1:2500))
glycoprotein G for HSV-1, Recombinant Protein (Cat# AAA72878)
Full Name
Recombinant biotinylated antigen glycoprotein G for HSV-1
Pricing
Application Data
(The purity of PAD6 was determined to be >95% by densitometry. Approx. MW 100 kDa .)
PAD6, Recombinant Protein (Cat# AAA72908)
Full Name
PAD6 recombinant protein
Gene Names
PADI6; hPADVI
Applications
Western Blot
Pricing
Application Data
PI4KA, Recombinant Protein (Cat# AAA73066)
Full Name
PI4KA, Active
Gene Names
PI4KA; PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
Applications
Kinase Assay
Pricing
Application Data
PNMT, Recombinant Protein (Cat# AAA73068)
Full Name
PNMT, Active
Gene Names
PNMT; PENT; PNMTase
Applications
Methyltransferase Assay
Pricing
Application Data
PRMT8, Recombinant Protein (Cat# AAA73073)
Full Name
PRMT8, Active
Gene Names
PRMT8; HRMT1L3; HRMT1L4
Applications
Methyltransferase Assay
Pricing
Application Data
(The purity of AMPK was determined to be >85% by densitometry.A1 approx. MW 68-75kDaB1 approx. MW 38kDaG1 approx. MW 40kDa)
AMPK, Recombinant Protein (Cat# AAA73076)
Full Name
AMPK (A1/B1/G1), Active
Gene Names
PRKAA1; AMPK; AMPKa1
Pricing
Application Data
PDE5A, Recombinant Protein (Cat# AAA73104)
Full Name
PDE5A, Active
Gene Names
PDE5A; CN5A; PDE5; CGB-PDE
Applications
PDE Assay
Pricing
Application Data
BCR-RET, Recombinant Protein (Cat# AAA73112)
Full Name
BCR-RET Mutant, Active
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Applications
Kinase Assay
Pricing
Application Data
TPM3-ROS1, Recombinant Protein (Cat# AAA73117)
Full Name
TPM3-ROS1 Mutant, Active
Gene Names
TPM3; TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5
Applications
Kinase Assay
Pricing
Application Data
RhoD, Recombinant Protein (Cat# AAA73119)
Full Name
RhoD (Mature form), Active
Gene Names
RHOD; Rho; ARHD; RHOM; RHOHP1
Applications
Western Blot
Pricing
Application Data
SETD1A, Recombinant Protein (Cat# AAA73129)
Full Name
SETD1A (KMT2F), Active
Gene Names
SETD1A; Set1; KMT2F; Set1A
Applications
Methyltransferase Assay
Pricing
Application Data
(The purity of SIRT5 was determined to be >75% by densitometry. Approx. MW 39kDa)
SIRT5, Recombinant Protein (Cat# AAA73141)
Full Name
SIRT5, Active
Gene Names
SIRT5; SIR2L5
Applications
SIRT Assay
Purity
>75%
Pricing
Application Data
(The purity of STAT5B protein was determined to be >90% by densitometry. Apporx. MW 95kDa.)
STAT5B, Recombinant Protein (Cat# AAA73148)
Full Name
STAT5B Protein
Gene Names
STAT5B; STAT5
Applications
SDS-PAGE
Purity
>90%
Pricing
Purity Data
(The purity of Dog ACE2 (18-739) Protein was determined to be >90% by densitometry. approx. MW 150kDa)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ABL1 (Y253F), Recombinant Protein (Cat# AAA73191)
Full Name
ABL1 (Y253F), Active
Applications
Kinase Assay
Pricing
Application Data
FLT3, Recombinant Protein (Cat# AAA72986)
Full Name
FLT3 Mutant (Y591-V592insVDFREYEYD), Active
Gene Names
FLT3; FLK2; STK1; CD135; FLK-2
Applications
Kinase Assay
Pricing
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.