Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
484 results for "Signal Transduction" - showing 450-484
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).)
C-CBL, Monoclonal Antibody (Cat# AAA29909)
Full Name
C-CBL Antibody
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(PINK1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
PINK1, Monoclonal Antibody (Cat# AAA28638)
Full Name
PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
IF (Immunofluorescence)
(Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody (C-term). AAA28702 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
CD38, Polyclonal Antibody (Cat# AAA28702)
Full Name
CD38 Antibody (C-term)
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human Spleen tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
KLF4, Polyclonal Antibody (Cat# AAA28755)
Full Name
KLF4 Antibody (N-term C74)
Gene Names
KLF4; EZF; GKLF
Reactivity
Human, mouse
Applications
WB, EIA, IF, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.)
NEK2, Polyclonal Antibody (Cat# AAA28694)
Full Name
NEK2 Antibody (Center)
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
WB, EIA, IF, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
Phospho-Histone H3-S10/T11, Polyclonal Antibody (Cat# AAA28391)
Full Name
Phospho-Histone H3-S10/T11 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IDH1, Polyclonal Antibody (Cat# AAA28676)
Full Name
IDH1 Antibody (Center)
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)
FGFR2, Polyclonal Antibody (Cat# AAA28728)
Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)
HDAC2, Polyclonal Antibody (Cat# AAA28801)
Full Name
HDAC2 Antibody (C-term)
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9/K14/K18/K23/K27 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Acetyl-Histone H3-K9/K14/K18/K23/K27, Polyclonal Antibody (Cat# AAA28357)
Full Name
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF, ChIP
Purity
Affinity purification
Pricing
WB (Western Blot)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
PDIA6, Polyclonal Antibody (Cat# AAA28792)
Full Name
PDIA6 Antibody (Center K159)
Gene Names
PDIA6; P5; ERP5; TXNDC7
Reactivity
Human, mouse, rat
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of GALE using anti-GALE antibody (AAA19135).GALE was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALE Antibody (AAA19135) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GALE, Polyclonal Antibody (Cat# AAA19135)
Full Name
Anti-GALE Picoband antibody
Gene Names
GALE; SDR1E1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistochemistry)
(ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ATP5B, Polyclonal Antibody (Cat# AAA28724)
Full Name
ATP5B Antibody (Center)
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HepG2 cells using AAA26922 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
14-3-3 protein beta/alpha, Polyclonal Antibody (Cat# AAA26922)
Full Name
Rabbit anti-human 14-3-3 protein beta/alpha polyclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, mouse, rat
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
Application Data
(Overlay histogram showing SY5Y cells stained with AAA27008 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (10ug/1x10^6cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
GFAP, Monoclonal Antibody (Cat# AAA27008)
Full Name
GFAP Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G purified
Pricing
WB (Western Blot)
(Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) (Origene Technologies).)
RYK, Polyclonal Antibody (Cat# AAA28767)
Full Name
RYK Antibody
Gene Names
RYK; JTK5; RYK1; JTK5A; D3S3195
Reactivity
Human, mouse, rat
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of NSF using anti-NSF antibody (AAA19136).NSF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSF Antibody (AAA19136) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
NSF, Polyclonal Antibody (Cat# AAA19136)
Full Name
Anti-NSF Picoband antibody
Gene Names
NSF; SKD2; SEC18
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
WB (Western Blot)
(TFAM Antibody (C-term) western blot analysis in Hela,Jurkat,K562,MCF-7 cell line lysates (35ug/lane).This demonstrates the TFAM antibody detected the TFAM protein (arrow).)
TFAM, Polyclonal Antibody (Cat# AAA28696)
Full Name
TFAM Antibody (C-term)
Gene Names
TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
Reactivity
Human
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(HAS2 Antibody (Center) (AAA28714) flow cytometric analysis of K562 cells (bottom histogram) compared to a Rabbit IgG Isotype control (negative control-top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HAS2, Polyclonal Antibody (Cat# AAA28714)
Full Name
HAS2 Antibody (Center)
Reactivity
Human, mouse
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Overlay histogram showing HL-60 cells stained with AAA27009 (red line) at 1:500. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C.The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.)
CD31, Monoclonal Antibody (Cat# AAA27009)
Full Name
CD31 Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of HBD using anti-HBD antibody (AAA19142).HBD was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HBD Antibody (AAA19142) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
HBD, Polyclonal Antibody (Cat# AAA19142)
Full Name
Anti-HBD Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of AMD1 using anti-AMD1 antibody (AAA19177).AMD1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-AMD1 Antibody (AAA19177) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
AMD1/Adometdc, Polyclonal Antibody (Cat# AAA19177)
Full Name
Anti-AMD1/Adometdc Antibody
Gene Names
AMD1; AMD; SAMDC; ADOMETDC
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (AAA19176).CPI17 alpha was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CPI17 alpha Antibody (AAA19176) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CPI17 alpha, Polyclonal Antibody (Cat# AAA19176)
Full Name
Anti-CPI17 alpha Picoband Antibody
Gene Names
PPP1R14A; CPI17; CPI-17; PPP1INL
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
SHH, Polyclonal Antibody (Cat# AAA23516)
Full Name
SHH antibody - N-terminal region
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of DYNLT1 using anti-DYNLT1 antibody (AAA19175).DYNLT1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DYNLT1 Antibody (AAA19175) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
DYNLT1, Polyclonal Antibody (Cat# AAA19175)
Full Name
Anti-DYNLT1 Picoband antibody
Gene Names
DYNLT1; CW-1; TCTEL1; tctex-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of GALNS using anti-GALNS antibody (AAA19164).GALNS was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALNS Antibody (AAA19164) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GALNS, Antibody (Cat# AAA19164)
Full Name
Anti-GALNS Picoband antibody
Gene Names
GALNS; GAS; MPS4A; GalN6S; GALNAC6S
Reactivity
Reacts with: Human, Mouse, Rat
Applications
WB, EIA
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (AAA19167).Cyclophilin B was detected in paraffin-embedded section of rat thyroid gland tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cyclophilin B Antibody (AAA19167) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cyclophilin B, Polyclonal Antibody (Cat# AAA19167)
Full Name
Anti-Cyclophilin B Picoband Antibody
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)
S100B, Polyclonal Antibody (Cat# AAA28759)
Full Name
S100B Antibody
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat (Predicted: Rabbit)
Applications
WB, IHC-P-Leica, FC/FACS, IHC, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Lyn using anti-Lyn antibody (AAA19150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 293T whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lyn antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lyn at approximately 59KD. The expected band size for Lyn is at 59KD.)
Lyn, Polyclonal Antibody (Cat# AAA19150)
Full Name
Anti-Lyn Picoband Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry)
(SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SOCS1, Polyclonal Antibody (Cat# AAA28752)
Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)
Ran, Polyclonal Antibody (Cat# AAA19131)
Full Name
Anti-Ran Picoband Antibody
Gene Names
RAN; TC4; Gsp1; ARA24
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of MCF-7 cell stained with BHLH3 Antibody (N-term). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BHLH3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).BHLH3 immunoreactivity is localized to nucleus significantly.)
BHLH3, Polyclonal Antibody (Cat# AAA28734)
Full Name
BHLH3 Antibody (N-term)
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse
Applications
FC, IF, WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of GAPDH Antibody (C-term R248) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
GAPDH, Polyclonal Antibody (Cat# AAA28693)
Full Name
GAPDH Antibody (C-term R248)
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)
SRC, Monoclonal Antibody (Cat# AAA28639)
Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
