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10000 results for "Recombinant Proteins" - showing 8100-8150


product-image-AAA48246_IF13.jpg IF (Immunofluorescence) (ICC/IF analysis of CDC37 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDC37 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

CDC37, Monoclonal Antibody (Cat# AAA48246)

Full Name
CDC37 antibody
Gene Names
CDC37; P50CDC37
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
product-image-AAA48011_IF13.jpg IF (Immunofluorescence) (ICC/IF analysis of BLyS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BLyS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green))

BLyS, Monoclonal Antibody (Cat# AAA48011)

Full Name
BLyS antibody
Gene Names
TNFSF13B; DTL; BAFF; BLYS; CD257; TALL1; THANK; ZTNF4; TALL-1; TNLG7A; TNFSF20
Reactivity
Human, Rat
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
product-image-AAA255276_WB13.png WB (Western Blot) (Anti-CD3D mouse monoclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Molt-4 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:24 kDa)

CD3D, Monoclonal Antibody (Cat# AAA255276)

Full Name
Anti-CD3D Antibody, Mouse Monoclonal
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
product-image-AAA257295_WB13.png WB (Western Blot) (Anti-Erbb2 rabbit polyclonal antibody at 1:500 dilutionLane A: SKBR3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:138 kDaObserved band size:190 kDa)

Her2/ERBB2, Polyclonal Antibody (Cat# AAA257295)

Full Name
Anti-Her2/ERBB2 Antibody, Rabbit Polyclonal
Reactivity
Rat
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
product-image-AAA107411_WB13.jpg WB (Western Blot) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant USP5 protein (Right) or empty vector (Left) detected with USP5 antibody)

USP5, Monoclonal Antibody (Cat# AAA107411)

Full Name
USP5 antibody
Gene Names
USP5; ISOT
Applications
Immunohistochemistry
Purity
USP5 antibody was purified by affinity chromatography.
Pricing
product-image-AAA72867_AD13.jpg Application Data (This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.977)

Phl p 7, Recombinant Protein (Cat# AAA72867)

Full Name
Recombinant allergen Phl p 7 apo-protein for Phleum pratense (Timothy grass pollen)
Purity
15% SDS-PAGE
Pricing
product-image-AAA74758_IF11.jpg IF (Immunofluorescence) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant CAPN9 protein using CAPN9 antibody)

CAPN9, Monoclonal Antibody (Cat# AAA74758)

Full Name
CAPN9 antibody
Gene Names
CAPN9; GC36; nCL-4
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
CAPN9 antibody was purified by affinity chromatography.
Pricing
product-image-AAA46623_IHC13.jpg IHC (Immunohiostchemistry) (ORM1 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- ORM1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method.)

ORM1, Polyclonal Antibody (Cat# AAA46623)

Full Name
Anti-ORM1 Antibody
Gene Names
ORM1; ORM; AGP1; AGP-A; HEL-S-153w
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA48040_IHC13.jpg IHC (Immunohiostchemistry) (Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

DACT3, Monoclonal Antibody (Cat# AAA48040)

Full Name
DACT3 antibody
Gene Names
DACT3; RRR1; DAPPER3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
product-image-AAA134294_SDS_PAGE11.jpg SDS-PAGE

Ribonuclease A4 (RNASE4), Recombinant Protein (Cat# AAA134294)

Full Name
Recombinant Ribonuclease A4 (RNASE4)
Gene Names
RNASE4; RAB1; RNS4
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 90%
Pricing
product-image-AAA60138_WB13.jpg WB (Western Blot) (Recombinant PRMT4 (CARM1) protein activity assay 0.5 ug Histone H3.1 were incubated with 0, 0.05 ug, 0.1 ug, 0.2 ug PRMT4 (CARM1) in 30 ul reaction system for 2 hours at room temperature, respectively. 6 ul reaction samples were loaded and run on a 13% SDS-PAGE gel. Western blot was used to detect the generation of products (H3R17me2a antibody). The western blot results show that histone H3 is di-methylated on its Arginine 17 by PRMT4 (CARM1).)

PRMT4, Recombinant Protein (Cat# AAA60138)

Full Name
Recombinant PRMT4 (CARM1) protein
Gene Names
CARM1; PRMT4
Pricing
product-image-AAA255731_IP13.png IP (Immunoprecipitation) (AIF1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)

Iba1, Monoclonal Antibody (Cat# AAA255731)

Full Name
Recombinant Anti-Iba1 Antibody, Rabbit Monoclonal
Gene Names
AIF1; IBA1; IRT1; AIF-1; IRT-1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
product-image-AAA152074_SDS_PAGE13.jpg SDS-PAGE (SDS-PAGE)

Triggering Receptor Expressed On Myeloid Cells 1 (TREM1), Recombinant Protein (Cat# AAA152074)

Full Name
Eukaryotic Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
Gene Names
TREM1; CD354; TREM-1
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
product-image-AAA51824_WB11.jpg WB (Western Blot) (Cell lysates of U87 MG(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Clusterin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.)

CLU / Clusterin, Monoclonal Antibody (Cat# AAA51824)

Full Name
Mouse Monoclonal [clone 1A11] (IgG1,k) to Human CLU / Clusterin
Gene Names
CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein G Purified
Pricing
product-image-AAA255399_WB11.png WB (Western Blot) (Anti-SAA4 rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:14 kDa)

SAA4, Monoclonal Antibody (Cat# AAA255399)

Full Name
Recombinant Anti-SAA4 Antibody, Rabbit Monoclonal
Gene Names
SAA4; CSAA; C-SAA
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
Protein A
Pricing
product-image-AAA254915_WB13.png WB (Western Blot) (Anti-BMP2 mouse monoclonal antibody at 1:500 dilutionLane A: Caco2 Whole Cell LysateLane B: THP1 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:39 kDa(We are unsure as to the identity of these extra bands.))

BMP-2, Monoclonal Antibody (Cat# AAA254915)

Full Name
Anti-BMP-2 Antibody, Mouse Monoclonal
Gene Names
BMP2; BDA2; BMP2A
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
product-image-AAA60179_AD13.jpg Application Data (MALDI-TOF for Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag 3 ?M HIF1A peptide was incubated with 300 nM PHD3 protein in 30 ?l reaction system containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 ?M 2-oxoglutarate, 100 ?M ascorbate and 50 ?M (NH4)2Fe(SO4)2·6H2O for 2 hours at 30 degree C. MALDI-TOF was used for detection.)

PHD3, Recombinant Protein (Cat# AAA60179)

Full Name
Recombinant PHD3 (EGLN3) protein, DYKDDDDK-Tag
Gene Names
EGLN3; PHD3; HIFPH3; HIFP4H3
Pricing
product-image-AAA48017_IF13.jpg IF (Immunofluorescence) (ICC/IF analysis of IL-33 in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IL-33 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

IL33, Monoclonal Antibody (Cat# AAA48017)

Full Name
IL33 antibody
Gene Names
IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
product-image-AAA48158_IF13.jpg IF (Immunofluorescence) (ICC/IF analysis of PARK7 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of PARK7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PARK7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

PARK7, Monoclonal Antibody (Cat# AAA48158)

Full Name
PARK7 antibody
Gene Names
PARK7; DJ1; DJ-1; GATD2; HEL-S-67p
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
product-image-AAA257143_WB13.png WB (Western Blot) (Anti-GLO1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:21 kDaObserved band size:23 kDa(We are unsure as to the identity of these extra bands.))

GLO1, Polyclonal Antibody (Cat# AAA257143)

Full Name
Anti-GLO1 Antibody, Rabbit Polyclonal
Gene Names
Glo1; GLY1; Qglo; Glo-1; Glo-1r; Glo-1s; Glo1-r; Glo1-s; AW550643; 0610009E22Rik; 1110008E19Rik; 2510049H23Rik
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
product-image-AAA255328_WB13.png WB (Western Blot) (Anti-SerpinF1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa(We are unsure as to the identity of these extra bands.))

PEDF, Polyclonal Antibody (Cat# AAA255328)

Full Name
Anti-PEDF Antibody, Rabbit Polyclonal
Gene Names
SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
product-image-AAA255681_IF11.png IF (Immunofluorescence) (Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSPH monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).)

PSPH, Monoclonal Antibody (Cat# AAA255681)

Full Name
Recombinant Anti-PSPH Antibody, Rabbit Monoclonal
Gene Names
PSPH; PSP; PSPHD
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
product-image-AAA214579_SDS_PAGE11.jpg SDS-PAGE (SDS-PAGE Analysis Purified CD9 Mouse Monoclonal Antibody (CD9/2343). Confirmation of Integrity and Purity of Antibody.)

CD9 (TSPAN29), Monoclonal Antibody (Cat# AAA214579)

Full Name
CD9 (TSPAN29)
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Pricing
product-image-AAA46484_IHC10.jpg IHC (Immunohistochemistry) (Anti- CYP1B1 Picoband antibody, AAA46484,IHC(P)IHC(P): Human Liver Cancer Tissue)

CYP1B1, Polyclonal Antibody (Cat# AAA46484)

Full Name
Anti-CYP1B1 Antibody
Gene Names
CYP1B1; CP1B; GLC3A; CYPIB1; P4501B1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
product-image-AAA76195_ELISA11.jpg ELISA (Biotinylated Human GARP&Latent TGF beta 1 Complex Protein ELISA DataImmobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex, His Tag with the EC50 of 44.0ng/ml determined by ELISA.)

GARP&Latent TGF beta 1 Complex, Recombinant Protein (Cat# AAA76195)

Full Name
Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein Recombinant
Gene Names
Lrrc32; Garp; AI426318; EG434215; D11S833Eh; D7H11S833E
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
product-image-AAA161790_BIOACTIVITY13.jpg Bioactivity (PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system through activating its specific membrane-anchored receptor (PRLR). PRL can bind to PRLR, inducing JAK2 association that leads to downstream activation of multiple pathways that include STAT3, STAT5, PI3K, AKT, and ERK. A functional binding ELISA assay was conducted to detect the interaction of recombinant zebrafish PRL and recombinant rat JAK2. Briefly, PRL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to JAK2 -coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant zebrafish PRL and recombinant rat JAK2 was shown in Figure 1, the EC50 for this effect is 0.95 ug/mL.)

Prolactin (PRL), Active Protein (Cat# AAA161790)

Full Name
Active Prolactin (PRL)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
product-image-AAA257111_IP8.png IP (Immunoprecipitation) (mC1QBP was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-mC1QBP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mC1QBP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size :34 kDa)

C1QBP, Polyclonal Antibody (Cat# AAA257111)

Full Name
Anti-C1QBP Antibody, Rabbit Polyclonal
Gene Names
C1qbp; P32; HABP1; gC1qBP; AA407365; AA986492; D11Wsu182e
Reactivity
Mouse
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
product-image-AAA151966_IHC13.jpg IHC (Immunohiostchemistry) (DAB staining on IHCP;Samples: Rat Testis Tissue;Primary Ab: 10ug/ml Rabbit AntiRat IL17C AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiRabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19))

Interleukin 17C (IL17C), Polyclonal Antibody (Cat# AAA151966)

Full Name
Polyclonal Antibody to Interleukin 17C (IL17C)
Reactivity
Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
product-image-AAA215198_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed, paraffin-embedded human Bladder stained with GLUT-1 Mouse Monoclonal Antibody (rGLUT1/2476).)

GLUT-1, Antibody (Cat# AAA215198)

Full Name
GLUT-1 (Tumor Progression and Mesothelioma Marker)
Gene Names
SLC2A1; CSE; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; SDCHCN; GLUT1DS
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
product-image-AAA76162_AD11.jpg Application Data (Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein SPR DataBiotinylated Human Fc gamma RIIIB (NA2) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 4.3?M as determined in SPR assay (Biacore T200).)

Fc gamma RIIIB/CD16b (NA2), Recombinant Protein (Cat# AAA76162)

Full Name
Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein Recombinant
Gene Names
FCGR3B; CD16; FCG3; CD16A; CD16b; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
product-image-AAA161697_BIOACTIVITY13.jpg Bioactivity (Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant human TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rhTIMP2 (MW: 51.75 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rhTIMP2 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )

Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161697)

Full Name
Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)
Gene Names
TIMP2; DDC8; CSC-21K
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
product-image-AAA127179_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of K562 cells using anti-LAIR1 antibody (AAA127179).Overlay histogram showing K562 cells stained with AAA127179 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LAIR1 Antibody (AAA127179, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

LAIR1, Polyclonal Antibody (Cat# AAA127179)

Full Name
Anti-LAIR1 Antibody Picoband
Gene Names
LAIR1; CD305; LAIR-1
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA153133_BIOACTIVITY11.jpg Bioactivity (One unit of enzyme activity is defined as the 1ug of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37 degree C. The specific activity of recombinant human CES3 is 700 pmol/min/ug.)

Carboxylesterase 3 (CES3), Active Protein (Cat# AAA153133)

Full Name
Active Carboxylesterase 3 (CES3)
Gene Names
CES3; ES31
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
product-image-AAA124588_IHC10.jpg IHC (Immunohistochemistry) (Figure 4. IHC analysis of PAX5 using anti-PAX5 antibody (AAA124588).PAX5 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PAX5 Antibody (AAA124588) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

PAX5/Bsap, Polyclonal Antibody (Cat# AAA124588)

Full Name
Anti-PAX5/Bsap Picoband antibody
Gene Names
PAX5; ALL3; BSAP
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA151547_IHC13.jpg IHC (Immunohiostchemistry) (DAB staining on IHCP;Samples: Human Cerebrum Tissue; Primary Ab: 20ug/ml Mouse AntiHuman NTProBNP AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

NTerminal ProBrain Natriuretic Peptide (NTProBNP), Monoclonal Antibody (Cat# AAA151547)

Full Name
Monoclonal Antibody to NTerminal ProBrain Natriuretic Peptide (NTProBNP)
Gene Names
NPPB; BNP
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
product-image-AAA151943_IHC13.jpg IHC (Immunohiostchemistry) (DAB staining on IHCP;Samples: Rat Adrenal gland Tissue;Primary Ab: 30ug/ml Rabbit AntiRat COL2a1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiRabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19))

Procollagen III CTerminal Propeptide (PIIICP), Polyclonal Antibody (Cat# AAA151943)

Full Name
Polyclonal Antibody to Procollagen III CTerminal Propeptide (PIIICP)
Reactivity
Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
product-image-AAA125552_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of 293T cells using anti-PDX1 antibody (AAA125552).Overlay histogram showing 293T cells stained with AAA125552 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDX1 Antibody (AAA125552, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PDX1, Polyclonal Antibody (Cat# AAA125552)

Full Name
Anti-PDX1 Antibody
Gene Names
PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125716_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A549 cells using anti-14-3-3 Gamma/YWHAG antibody (AAA125716).Overlay histogram showing A549 cells stained with AAA125716 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Gamma/YWHAG Antibody (AAA125716, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

14-3-3 gamma/YWHAG, Polyclonal Antibody (Cat# AAA125716)

Full Name
Anti-14-3-3 gamma/YWHAG Antibody
Gene Names
YWHAG; 14-3-3GAMMA
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125806_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of SiHa cells using anti-LY6E/SCA-2 antibody (AAA125806).Overlay histogram showing SiHa cells stained with AAA125806 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LY6E/SCA-2 Antibody (AAA125806, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

LY6E/SCA-2, Polyclonal Antibody (Cat# AAA125806)

Full Name
Anti-LY6E/SCA-2 Antibody
Gene Names
LY6E; RIGE; SCA2; RIG-E; SCA-2; TSA-1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA313520_WB10.jpg WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CYP2B6 at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.)

CYP2B6, Polyclonal Antibody (Cat# AAA313520)

Full Name
CYP2B6 Rabbit Polyclonal Antibody
Gene Names
CYP2B6; CPB6; EFVM; IIB1; P450; CYP2B; CYP2B7; CYP2B7P; CYPIIB6
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purification
Pricing
product-image-AAA126975_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Hela cells using anti-E2F1 antibody (AAA126975).Overlay histogram showing Hela cells stained with AAA126975 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-E2F1 Antibody (AAA126975, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

E2F1, Polyclonal Antibody (Cat# AAA126975)

Full Name
Anti-E2F1 Antibody Picoband
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA127486_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of RT4 cells using anti-OVGP1 antibody (AAA127486).Overlay histogram showing RT4 cells stained with AAA127486 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OVGP1 Antibody (AAA127486, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

OVGP1, Polyclonal Antibody (Cat# AAA127486)

Full Name
Anti-OVGP1 Antibody Picoband
Gene Names
OVGP1; EGP; OGP; MUC9; CHIT5
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA161681_BIOACTIVITY13.jpg Bioactivity (Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Many of leptin's effects are mediated through neuropeptide-containing neurons and neuropeptide receptors in the hypothalamus. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. A functional binding ELISA assay was conducted to detect the interaction of recombinant mouse LEP and recombinant human LEPR. Briefly, LEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LEPR-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-LEP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of of LEP and LEPR was shown in Figure 1, the EC50 for this effect is 0.26 ug/mL.)

Leptin (LEP), Active Protein (Cat# AAA161681)

Full Name
Active Leptin (LEP)
Gene Names
Lep; ob; obese
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
product-image-AAA258768_IF11.png IF (Immunofluorescence) (Immunofluorescence staining of CSF1R in THP-1 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CSF1R polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane.)

M-CSFR/CSF1R, Polyclonal Antibody (Cat# AAA258768)

Full Name
Anti-M-CSFR/CSF1R Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
product-image-AAA125687_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A431 cells using anti-TRIM24 antibody (AAA125687).Overlay histogram showing A431 cells stained with AAA125687 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRIM24 Antibody (AAA125687,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TRIM24, Polyclonal Antibody (Cat# AAA125687)

Full Name
Anti-TRIM24 Antibody
Gene Names
TRIM24; PTC6; TF1A; TIF1; RNF82; TIF1A; hTIF1; TIF1ALPHA
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA124630_IHC8.jpg IHC (Immunohistochemistry) (Figure 5. IHC analysis of WWOX using anti-WWOX antibody (AAA124630).WWOX was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-WWOX Antibody (AAA124630) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WWOX, Polyclonal Antibody (Cat# AAA124630)

Full Name
Anti-WWOX Picoband antibody
Gene Names
WWOX; FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA151794_IHC11.jpg IHC (Immunohistochemisry) (DAB staining on IHCP;Samples: Human Liver Tissue; Primary Ab: 10ug/ml Mouse AntiHuman ACVR2A AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Activin A Receptor Type II A (ACVR2A), Monoclonal Antibody (Cat# AAA151794)

Full Name
Monoclonal Antibody to Activin A Receptor Type II A (ACVR2A)
Gene Names
ACVR2A; ACVR2; ACTRII
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
product-image-AAA125747_IHC10.jpg IHC (Immunohistochemistry) (Figure 4. IHC analysis of Nefh using anti-Nefh antibody (AAA125747).Nefh was detected in paraffin-embedded section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Nefh Antibody (AAA125747) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Nefh, Polyclonal Antibody (Cat# AAA125747)

Full Name
Anti-Nefh Antibody
Gene Names
Nefh; Nfh; NF-H; NF200; mKIAA0845
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA127632_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of RT4 cells using anti-Synaptogyrin 1 antibody (AAA127632).Overlay histogram showing RT4 cells stained with AAA127632 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptogyrin 1 Antibody (AAA127632, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Synaptogyrin 1, Polyclonal Antibody (Cat# AAA127632)

Full Name
Anti-Synaptogyrin 1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA215237_AD13.jpg Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using TRAF1 Mouse Monoclonal Antibody (TRAF1/3298) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

TRAF1 (TNFR-Associated Factor 1), Monoclonal Antibody (Cat# AAA215237)

Full Name
TRAF1 (TNFR-Associated Factor 1) (Lymphomatoid Papulosis Marker)
Gene Names
TRAF1; EBI6; MGC:10353
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing

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