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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Recombinant Proteins" - showing 7950-8000
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded H1299 xenograft using Coronin 1B antibody at a dilution of 1:500)
Coronin 1B, Polyclonal Antibody (Cat# AAA107863)
Full Name
Coronin 1B antibody
Gene Names
CORO1B; CORONIN-2
Applications
Western Blot, Immunohistochemistry
Purity
Coronin 1B antibody was purified by antigen-affinity chromatography
Pricing
WB (Western Blot)
(Anti-STMN1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLane C: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:17 kDa(We are unsure as to the identity of these extra bands.))
Stathmin 1, Polyclonal Antibody (Cat# AAA257545)
Full Name
Anti-Stathmin 1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(All lanes : Anti-RBX1 at dilutionLane 1: Jurkat whole cell lysateLane 2: HepG2 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 12kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
RBX1, Monoclonal Antibody (Cat# AAA290892)
Full Name
RBX1 Antibody
Gene Names
RBX1; ROC1; RNF75; BA554C12.1
Reactivity
Human, Mouse
Predicted: Mouse
Predicted: Mouse
Applications
Western Blot
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
SDS-PAGE
(Figure. SDS-PAGE)
Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL), Recombinant Protein (Cat# AAA150677)
Full Name
Recombinant Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Gene Names
Tnfsf11; ODF; OPG; OPGL; RANKL; Ly109l; Trance
Applications
Western Blot
Purity
>90%
Pricing
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant TOMM34 protein (Right) or empty vector (Left) detected with TOMM34 antibody)
TOMM34, Monoclonal Antibody (Cat# AAA108022)
Full Name
TOMM34 antibody
Applications
Western Blot, Immunofluorescence
Purity
TOMM34 antibody was purified by affinity chromatography.
Pricing
FCM/FACS (Flow Cytometry)
(Above is the histogram of Hela cells stained with anti-Human B2MG mAb D3A2 and fluorescence labelled secondary antibody. Black line represents histogram of Hela cells stained with isotype control, anti-TB 38Kd antigen mAb B12F8)
Beta 2 Microglobulin, Monoclonal Antibody (Cat# AAA77900)
Full Name
mAb anti-Human Beta 2 Microglobulin
Reactivity
Human. Others not tested
Applications
ELISA
Purity
Protein G affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using NEDD9 antibody. Blue: DAPI for nuclear staining.)
NEDD9, Polyclonal Antibody (Cat# AAA37426)
Full Name
NEDD9 Polyclonal Antibody
Gene Names
NEDD9; CAS2; CASL; HEF1; CAS-L; CASS2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant DSTN protein using DSTN antibody)
DSTN, Monoclonal Antibody (Cat# AAA106436)
Full Name
DSTN antibody
Gene Names
DSTN; ADF; ACTDP; HEL32; bA462D18.2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
DSTN antibody was purified by affinity chromatography.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200 ugextracts of HL-60 cells, using 3 ug PSMA3 antibody (AAA37094). Western blot was performed from the immunoprecipitate using PSMA3 antibody (AAA37094) at a dilition of 1:1000.)
PSMA3, Polyclonal Antibody (Cat# AAA37094)
Full Name
PSMA3 Polyclonal Antibody
Gene Names
PSMA3; HC8; PSC3
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of OSTF1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of OSTF1 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human OSTF1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
OSTF1, Monoclonal Antibody (Cat# AAA48241)
Full Name
OSTF1 antibody
Gene Names
OSTF1; OSF; SH3P2; bA235O14.1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(Anti-ALK1 rabbit monoclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLane B: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:56 kDaObserved band size:41 kDa)
ALK-1, Monoclonal Antibody (Cat# AAA254300)
Full Name
Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal
Gene Names
ACVRL1; HHT; ALK1; HHT2; ORW2; SKR3; ALK-1; TSR-I; ACVRLK1
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
WB (Western Blot)
(Western blot analysis of extracts of mouse thymus, using ILF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
ILF3, Polyclonal Antibody (Cat# AAA37244)
Full Name
ILF3 Polyclonal Antibody
Gene Names
ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Anti-C1QBP rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:35 kDa)
C1QBP, Polyclonal Antibody (Cat# AAA257110)
Full Name
Anti-C1QBP Antibody, Rabbit Polyclonal
Gene Names
C1qbp; P32; HABP1; gC1qBP; AA407365; AA986492; D11Wsu182e
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-SULT2B1 rabbit monoclonal antibody at 1:500 dilutionLane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:41 kDa)
SULT2B1, Monoclonal Antibody (Cat# AAA255413)
Full Name
Recombinant Anti-SULT2B1 Antibody, Rabbit Monoclonal
Gene Names
SULT2B1; HSST2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of TPMT in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TPMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
TPMT, Monoclonal Antibody (Cat# AAA48100)
Full Name
TPMT antibody
Gene Names
TPMT; TPMTD
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
IP (Immunoprecipitation)
(AK2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-AK2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AK2 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)
AK2, Polyclonal Antibody (Cat# AAA255641)
Full Name
Anti-AK2 Antibody, Rabbit Polyclonal
Gene Names
AK2; ADK2; AK 2
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-SAE1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Hela Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:38 kDaObserved band size:38 kDa)
SAE1, Polyclonal Antibody (Cat# AAA255710)
Full Name
Anti-SAE1 Antibody, Rabbit Polyclonal
Gene Names
SAE1; AOS1; SUA1; UBLE1A; HSPC140
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of MEK1 in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse MEK1 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
MEK1, Monoclonal Antibody (Cat# AAA257133)
Full Name
Recombinant Anti-MEK1 Antibody, Rabbit Monoclonal
Gene Names
Map2k1; Mek1; MEKK1; MAPKK1; Prkmk1
Reactivity
Mouse
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
Application Data
(HTRF for Recombinant YTHDF1 (380-533) protein activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF1 (380-533) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
YTHDF1, Recombinant Protein (Cat# AAA60135)
Full Name
Recombinant YTHDF1 (380-533) protein
Gene Names
YTHDF1; C20orf21
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ARFGAP1 protein using ARFGAP1 antibody)
ARFGAP1, Monoclonal Antibody (Cat# AAA106319)
Full Name
ARFGAP1 antibody
Gene Names
ARFGAP1; ARF1GAP; HRIHFB2281
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
ARFGAP1 antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
UGDH, Monoclonal Antibody (Cat# AAA48236)
Full Name
UGDH antibody
Gene Names
UGDH; GDH; UGD; UDPGDH; UDP-GlcDH
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot)
(The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MBP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysate (40ug)Lane 2.: ORF73/HHV8-MBP tag Transfected 293T cell lysate (5ug))
ORF73/HHV8, Monoclonal Antibody (Cat# AAA48051)
Full Name
ORF73/HHV8 antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(Anti-CSNK2A1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:45 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))
CK2 alpha/CSNK2A1, Polyclonal Antibody (Cat# AAA257232)
Full Name
Anti-CK2 alpha/CSNK2A1 Antibody, Rabbit Polyclonal
Gene Names
Csnk2a1; Csnk2a1-rs4
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-MIF rabbit polyclonal antibody at 1:500 dilutionLane A: U937 Whole Cell LysateLane B: HL60 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: Raw264.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:12 kDaObserved band size:12 kDa)
MIF, Polyclonal Antibody (Cat# AAA257075)
Full Name
Anti-MIF Antibody, Rabbit Polyclonal
Gene Names
Mif; GIF; Glif
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Western blot analysis of 20 ug of whole cell lysate (A: human ESC) using a 5 % SDS PAGE gel and Integrin alpha 6 antibody at a dilution of 1:2000)
Integrin alpha 6, Polyclonal Antibody (Cat# AAA107934)
Full Name
Integrin alpha 6 antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human, Mouse, Rat.
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Integrin alpha 6 antibody was purified by antigen-affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SSX1 protein using SSX1 antibody)
SSX1, Monoclonal Antibody (Cat# AAA106688)
Full Name
SSX1 antibody
Gene Names
SSX1; SSRC; CT5.1
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
SSX1 antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Anti-ALDH4A1 rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: K562 Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:62 kDaObserved band size:62 kDa(We are unsure as to the identity of these extra bands.))
ALDH4A1, Monoclonal Antibody (Cat# AAA255609)
Full Name
Recombinant Anti-ALDH4A1 Antibody, Rabbit Monoclonal
Gene Names
ALDH4A1; P5CD; ALDH4; P5CDh
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of KYNU in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KYNU monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
kynureninase/KYNU, Monoclonal Antibody (Cat# AAA255217)
Full Name
Recombinant Anti-kynureninase/KYNU Antibody, Rabbit Monoclonal
Gene Names
KYNU; KYNUU
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
IP (Immunoprecipitation)
(Mprdx5 was immunoprecipitated using:Lane A:0.5 mg Mouse lung tissue Lysate4 uL anti-Mprdx5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-Mprdx5 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size :16 kDa)
Peroxiredoxin 5, Polyclonal Antibody (Cat# AAA257180)
Full Name
Anti-Peroxiredoxin 5 Antibody, Rabbit Polyclonal
Gene Names
Prdx5; AOPP; PrxV; Pmp20; Prdx6; AOEB166
Reactivity
Mouse
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant KCNJ3 protein using KCNJ3 antibody)
KCNJ3, Monoclonal Antibody (Cat# AAA107464)
Full Name
KCNJ3 antibody
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunofluorescence
Purity
KCNJ3 antibody was purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant EFNA2 protein using EFNA2 antibody)
EFNA2, Monoclonal Antibody (Cat# AAA106205)
Full Name
EFNA2 antibody
Gene Names
EFNA2; ELF-1; EPLG6; LERK6; HEK7-L; LERK-6
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
EFNA2 antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Anti-SerpinF2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:55 kDa(We are unsure as to the identity of these extra bands.))
Alpha 2 Antiplasmin/SerpinF2, Polyclonal Antibody (Cat# AAA254810)
Full Name
Anti-Alpha 2 Antiplasmin/SerpinF2 Antibody, Rabbit Polyclonal
Gene Names
SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry)
(TCP1 theta was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- TCP1 theta Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
TCP1 theta, Polyclonal Antibody (Cat# AAA46641)
Full Name
Anti-TCP1 theta Antibody
Gene Names
CCT8; Cctq; PRED71; D21S246; C21orf112
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Syntaxin-binding 1 (STXBP1), Recombinant Protein (Cat# AAA244041)
Full Name
Recombinant Human Syntaxin-binding protein 1 (STXBP1)
Gene Names
STXBP1; P67; NSEC1; UNC18; RBSEC1; MUNC18-1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
WB (Western Blot)
(Anti-KCNIP3 rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse heart Tissue LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:28 kDaObserved band size:36 kDa)
CSEN, Polyclonal Antibody (Cat# AAA255790)
Full Name
Anti-CSEN Antibody, Rabbit Polyclonal
Gene Names
KCNIP3; CSEN; DREAM; KCHIP3
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Figure. Western blot analysis of IL-6 using anti- IL-6 antibody (AAA44957).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane : Recombinant Human IL-6 Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-6 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-6 at approximately 20KD. The expected band size for IL-6 is at 20KD.)
IL6, Polyclonal Antibody (Cat# AAA44957)
Full Name
Anti-human IL6 antibody
Gene Names
IL6; HGF; HSF; BSF2; IL-6; IFNB2
Reactivity
Human
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot for Recombinant KAT5 protein activity 2 ?g Recombinant Mononucleosomes H3.1 were incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant KAT5 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody and H3 antibody . Western blot was used to detect the production of acetylated histone H4. H3 was detected as a loading control.)
KAT5, Recombinant Protein (Cat# AAA60202)
Full Name
Recombinant KAT5 protein
Gene Names
KAT5; TIP; ESA1; PLIP; TIP60; cPLA2; HTATIP; ZC2HC5; HTATIP1
Pricing
Application Data
(HTRF assay for PIM2 activity 1 uM STK S3 substrate was incubated with different concentrations of PIM2 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(Biotinylated Human LAIR2/CD306 Protein SPR DataBiotinylated Human LAIR2, His-Avi Tag immobilized on CM5 Chip can bind Native Human Collagen I, No Tag with an affinity constant of 1.80 nM as determined in SPR assay (Biacore T200).)
LAIR2/CD306, Recombinant Protein (Cat# AAA76179)
Full Name
Biotinylated Human LAIR2/CD306 Protein Recombinant
Gene Names
LAIR2; CD306
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Application Data
(Biotinylated Human DNAM-1/CD226 Protein SPR DataHuman CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).)
DNAM-1/CD226, Recombinant Protein (Cat# AAA76238)
Full Name
Biotinylated Human DNAM-1/CD226 Protein Recombinant
Gene Names
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Bioactivity
(PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system through activating its specific membrane-anchored receptor (PRLR). A functional ELISA assay was conducted to detect the interaction of recombinant human PRL and recombinant human PRLR. Briefly, PRL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PRLR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PRL and recombinant human PRLR was shown in Figure 1, the EC50 for this effect is 0.05 ug/mL.)
Prolactin (PRL), Active Protein (Cat# AAA161792)
Full Name
Active Prolactin (PRL)
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
ELISA
(Biotinylated Human GITR Ligand/TNFSF18 Trimer Protein (Primary Amine Labeling) ELISA DataImmobilized Human GITR, His Tag at 1?g/ml (100?l/Well). Dose response curve for Biotinylated Human GITR Ligand Trimer, His Tag with the EC50 of 0.2?g/ml determined by ELISA.)
GARP&Latent TGF beta 1 Complex, Recombinant Protein (Cat# AAA76228)
Full Name
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
ELISA
(Biotinylated Human SIRP alpha V2/CD172a Protein ELISA DataImmobilized Biotinylated Human SIRP alpha V2, His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.11?ng/ml determined by ELISA.)
SIRP alpha V2/CD172a, Recombinant Protein (Cat# AAA76222)
Full Name
Biotinylated Human SIRP alpha V2/CD172a Protein Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Amino Acid Info
Eukaryotic Cluster Of Differentiation 8a (CD8a), Protein (Cat# AAA153195)
Full Name
Eukaryotic Cluster Of Differentiation 8a (CD8a)
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IP (Immunoprecipitation)
(PRKD2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate4 uL anti-PRKD2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRKD2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 100 kDaObserved band size: 100 kDa)
Protein Kinase D2/PRKD2, Polyclonal Antibody (Cat# AAA255458)
Full Name
Anti-Protein Kinase D2/PRKD2 Antibody, Rabbit Polyclonal
Gene Names
PRKD2; PKD2; HSPC187; nPKC-D2
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
IP (Immunoprecipitation)
(LRPAP1 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate2 uL anti-LRPAP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LRPAP1 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 41 kDa)
LRPAP1, Monoclonal Antibody (Cat# AAA255325)
Full Name
Recombinant Anti-LRPAP1 Antibody, Rabbit Monoclonal
Gene Names
LRPAP1; RAP; MRAP; A2RAP; HBP44; A2MRAP
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-PRSS8 antibody (AAA127308).Overlay histogram showing MCF-7 cells stained with AAA127308 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS8 Antibody (AAA127308, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRSS8, Polyclonal Antibody (Cat# AAA127308)
Full Name
Anti-PRSS8 Antibody Picoband
Gene Names
PRSS8; CAP1; PROSTASIN
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
Cadherin, Osteoblast (CDHOB), Monoclonal Antibody (Cat# AAA151682)
Full Name
Monoclonal Antibody to Cadherin, Osteoblast (CDHOB)
Gene Names
CDH11; OB; CAD11; CDHOB; OSF-4
Reactivity
Human, Rat, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
ICC (Immunocytochemistry)
(Anti- Parkin Picoband antibody, AAA46524, ICCICC: NEURO-2alpha Cell)
Parkin, Polyclonal Antibody (Cat# AAA46524)
Full Name
Anti-Parkin Antibody
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PAI1 using anti-PAI1 antibody (AAA124586).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat small intestine tissue lysates,Lane 2: rat kidney tissue lysates,Lane 3: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAI1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAI1 at approximately 45-50KD. The expected band size for PAI1 is at 45KD.)
PAI1, Polyclonal Antibody (Cat# AAA124586)
Full Name
Anti-PAI1 Picoband antibody
Gene Names
Serpine1; Pai1; PAI1A; Planh; Pai1aa; RATPAI1A
Reactivity
Mouse, Rat
Applications
Direct ELISA, Western Blot
Purity
Immunogen affinity purified.
Pricing