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2045 results for " Antigen" - showing 1500-1550

WB (Western Blot) (FGFR2 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the FGFR2 antibody detected the FGFR2 protein (arrow).)

FGFR2, Polyclonal Antibody (Cat# AAA28728)

Full Name
FGFR2 Antibody (N-term)
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, mouse, rat, monkey
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry) (Overlay histogram showing Raji cells stained with AAA28060 (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

CD45, Monoclonal Antibody (Cat# AAA28060)

Full Name
CD45 Monoclonal Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein A purified
Pricing
IHC (Immunohistchemistry) (At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

BCLAF1, Polyclonal Antibody (Cat# AAA31422)

Full Name
Phospho-BCLAF1 (Ser531) Antibody
Gene Names
BCLAF1; BTF; bK211L9.1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)

CD172a, Monoclonal Antibody (Cat# AAA11967)

Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS, WB
Pricing
Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)

CD172a, Monoclonal Antibody (Cat# AAA11968)

Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS, WB
Pricing
IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)

HDAC2, Polyclonal Antibody (Cat# AAA28801)

Full Name
HDAC2 Antibody (C-term)
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

SRF, Polyclonal Antibody (Cat# AAA31361)

Full Name
Phospho-SRF (Ser103) Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (89%), Dog (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

DUSP1/MKP1, Polyclonal Antibody (Cat# AAA31363)

Full Name
Phospho-DUSP1/MKP1 (Ser359) Antibody
Gene Names
DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (92%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

CD11b, Monoclonal Antibody (Cat# AAA11889)

Full Name
RAT ANTI MOUSE CD11b:FITC
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
FC/FACS
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

PFKFB3, Polyclonal Antibody (Cat# AAA31288)

Full Name
Phospho-PFKFB3 (Ser461) Antibody
Gene Names
PFKFB3; PFK2; IPFK2
Reactivity
Human, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot) (Applications: Western BlotSample: Rat PFCDetection: Protein Simple, Simple Western size-based assay"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate.")

CD10, Polyclonal Antibody (Cat# AAA11606)

Full Name
Anti-CD10 Antibody
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry) (Annexin II Antibody for IHC in human colon tissue)

Annexin II, Polyclonal Antibody (Cat# AAA31078)

Full Name
Annexin II Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot) (Western blot analysis of Catenin 1 Antibody expression in MCF-7 cells lysates.The lane on the left is treated with the antigen-specific peptide..The lane on the left is treated with the antigen-specific peptide.)

Catenin alpha1, Polyclonal Antibody (Cat# AAA31080)

Full Name
Catenin alpha1 Antibody
Gene Names
CTNNA1; MDPT2; CAP102
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry) (AAA31091 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

VEGFR2, Polyclonal Antibody (Cat# AAA31091)

Full Name
VEGFR2 Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot) (Formalin-fixed and paraffin-embedded human brain tissue reacted with PDIA6 Antibody (Center K159), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

PDIA6, Polyclonal Antibody (Cat# AAA28792)

Full Name
PDIA6 Antibody (Center K159)
Gene Names
PDIA6; P5; ERP5; TXNDC7
Reactivity
Human, mouse, rat
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

R-Ras, Polyclonal Antibody (Cat# AAA31465)

Full Name
Phospho-R-Ras (Tyr66) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Dog (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)

CD11b, Monoclonal Antibody (Cat# AAA11991)

Full Name
MOUSE ANTI HUMAN CD11b
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Baboon, Cynomolgus monkey, Rhesus Monkey
Applications
FC/FACS, IP
Pricing
FCM (Flow Cytometry) (Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

EEF1A1, Monoclonal Antibody (Cat# AAA30467)

Full Name
EEF1A1 Antibody
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS, IP
Purity
ProA affinity purified
Pricing
Application Data

Ep-CAM /CD326, Monoclonal Antibody (Cat# AAA13831)

Full Name
Ep-CAM /CD326 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

MUC1 / EMA /CD227, Monoclonal Antibody (Cat# AAA13843)

Full Name
MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
IHC (Immunohistochemistry) (ATP5B Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

ATP5B, Polyclonal Antibody (Cat# AAA28724)

Full Name
ATP5B Antibody (Center)
Gene Names
ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26922 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

14-3-3 protein beta/alpha, Polyclonal Antibody (Cat# AAA26922)

Full Name
Rabbit anti-human 14-3-3 protein beta/alpha polyclonal Antibody
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1
Reactivity
Human, mouse, rat
Applications
EIA, WB, IHC
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
FCM (Flow Cytometry) (CDK4 Antibody (C-term) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

CDK4, Polyclonal Antibody (Cat# AAA28747)

Full Name
CDK4 Antibody (C-term)
Gene Names
CDK4; CMM3; PSK-J3
Reactivity
Human
Applications
FC/FACS, EIA, IHC, WB, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data

Interferon-a Receptor, Type I, Subunit I (Ser535,539), Polyclonal Antibody (Cat# AAA14231)

Full Name
Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I
Gene Names
IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC
Reactivity
Rat
Applications
WB, IHC
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Pricing
IF (Immunofluorescence) (Immunostaining of mixed neuron/glial cultures stained with anti-UCHL1 antibody (AAA14227), green, 1:500) and rabbit anti-GFAP antibody , red, 1:1000. The blue stains nuclear DNA. The anti-UCHL1 stains strongly the cell body and dendrites of neurons, while anti-GFAP specifically labels astrocytes.)

Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck, Polyclonal Antibody (Cat# AAA14227)

Full Name
Anti-Ubiquitin C Terminal Hydrolase 1 (UCHL1)
Gene Names
UCHL1; NDGOA; PARK5; PGP95; PGP9.5; Uch-L1; HEL-117; PGP 9.5
Reactivity
Bovine, Human, Mouse, Pig, Rat
Expected Reactivity: Canine, Feline, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole
Applications
WB, IF
Purity
Total IgY fraction
Pricing
FCM (Flow Cytometry) (Overlay histogram showing Raji cells stained with AAA27027 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

CD19, Monoclonal Antibody (Cat# AAA27027)

Full Name
CD19 Monoclonal Antibody
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein A Purified
Pricing
IHC (Immunohistochemistry) (AAA31132 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

EFNB2, Polyclonal Antibody (Cat# AAA31132)

Full Name
EFNB2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry) (AAA30925 at 1/100 staining human bone tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MCM5, Polyclonal Antibody (Cat# AAA30925)

Full Name
MCM5 Antibody
Gene Names
MCM5; CDC46; MGORS8; P1-CDC46
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-ICAM-1 (Tyr512) Antibody. Lane 1: VERO cells, treated with blocking peptideLane 2: VERO cellsLane 3: A2780 cells.)

ICAM-1, Polyclonal Antibody (Cat# AAA30979)

Full Name
Phospho-ICAM-1 (Tyr512) Antibody
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC, IF, ICC, EIA
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

EEF1A2, Polyclonal Antibody (Cat# AAA31289)

Full Name
Phospho-EEF1A2 (Ser358) Antibody
Gene Names
EEF1A2; HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation) (MAP2K3 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg HeLa Whole Cell Lysate 2 uL anti-MAP2K3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MAP2K3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :39 kDa)

MEK3/MKK3, Polyclonal Antibody (Cat# AAA27720)

Full Name
Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse
Applications
WB, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in rat liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

HMGB1, Polyclonal Antibody (Cat# AAA27719)

Full Name
Anti-HMGB1 Antibody, Rabbit Polyclonal
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry) (CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

CD44, Monoclonal Antibody (Cat# AAA14166)

Full Name
Anti-CD44 Mouse mAb
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human
Applications
IHC, WB, IF, FC/FACS
Purity
Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Pricing
IHC (Immunohistchemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

BAG3, Polyclonal Antibody (Cat# AAA31421)

Full Name
Phospho-BAG3 (Tyr457) Antibody
Gene Names
BAG3; BIS; BAG-3; CAIR-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NACA, Polyclonal Antibody (Cat# AAA31427)

Full Name
Phospho-NACA (Thr159) Antibody
Gene Names
NACA; HSD48; NACA1; skNAC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (Figure 7. IHC analysis of Periostin using anti-Periostin antibody (AAA19149).Periostin was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Periostin Antibody (AAA19149) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Periostin, Polyclonal Antibody (Cat# AAA19149)

Full Name
Anti-Periostin Picoband Antibody
Gene Names
Postn; Plf
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

MUC1, Monoclonal Antibody (Cat# AAA30155)

Full Name
MUC1 Antibody
Gene Names
MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

GAPDH, Polyclonal Antibody (Cat# AAA13657)

Full Name
Goat anti-GAPDH (Internal) Antibody
Gene Names
GAPDH; G3PD; GAPD; HEL-S-162eP
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
EIA, WB, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Smad5, Polyclonal Antibody (Cat# AAA31331)

Full Name
Phospho-Smad5 (Ser463/Ser465) Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot) (Anti-GP73 rabbit polyclonal antibody at 1:500 dilution Lane A: GOLM1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size: kDa)

GOLPH2/GOLM1, Polyclonal Antibody (Cat# AAA27751)

Full Name
Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal
Gene Names
GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3
Reactivity
Human
Applications
WB, EIA, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

CD68, Monoclonal Antibody (Cat# AAA12245)

Full Name
RAT ANTI MOUSE CD68:Endotoxin Low
Gene Names
Cd68; Lamp4; gp110; Scard1
Applications
FC/FACS, IF, IP, WB
Pricing
WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)

Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, EIA, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26901)

Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26899)

Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

TLK1, Polyclonal Antibody (Cat# AAA31385)

Full Name
Phospho-TLK1 (Ser741) Antibody
Gene Names
TLK1; PKU-beta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry) (Overlay histogram showing HepG2 cells stained with AAA28062 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

CD63, Monoclonal Antibody (Cat# AAA28062)

Full Name
CD63 Monoclonal Antibody
Gene Names
Cd63; ME491; C75951; Tspan30
Reactivity
Human, Rabbit
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G purified
Pricing
WB (Western Blot) (Western Blot (WB)Host: RabbitTarget: SOX10Positive control (+): Rat brain (R-BR)Negative control (-): Mouse intestine (M-IN) Antibody concentration: 1ug/ml)

SOX10, Polyclonal Antibody (Cat# AAA23423)

Full Name
SOX10 Antibody - middle region
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Reactivity: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

PKM, Monoclonal Antibody (Cat# AAA27048)

Full Name
PKM Monoclonal Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

KSR2, Polyclonal Antibody (Cat# AAA31343)

Full Name
Phospho-KSR2 (Thr497) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (91%), Xenopus (91%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation) (Immunoprecipitation(IP) of CRYAB by using monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CRYAB antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.)

CRYAB / Alpha B Crystallin, Monoclonal Antibody (Cat# AAA12373)

Full Name
Mouse Monoclonal [clone 6D11] (IgG1) to Human CRYAB / Alpha B Crystallin
Gene Names
CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Reactivity
Human, Monkey, Rat
Applications
IHC - Paraffin, IF, WB, IP, FC/FACS
Purity
Protein A/G Purified
Pricing

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