Search results for "Recombinant Proteins"

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PRMT8 antibody (AAA19321).Overlay histogram showing THP-1 cells stained with AAA19321 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT8 Antibody (AAA19321,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PRMT8, Polyclonal Antibody (Cat# AAA19321)

Full Name

Anti-PRMT8 Antibody

Gene Names

PRMT8; HRMT1L3; HRMT1L4

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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MRPS25, Polyclonal Antibody (Cat# AAA19639)

Full Name

Anti-MRPS25 Antibody Picoband

Gene Names

MRPS25; RPMS25; MRP-S25

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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NOLA1/GAR1, Polyclonal Antibody (Cat# AAA19579)

Full Name

Anti-NOLA1/GAR1 Antibody Picoband

Gene Names

GAR1; NOLA1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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BCKDHA, Polyclonal Antibody (Cat# AAA19523)

Full Name

Anti-BCKDHA Antibody Picoband

Gene Names

BCKDHA; MSU; MSUD1; OVD1A; BCKDE1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-C18orf22/RBFA antibody (AAA19984).Overlay histogram showing MCF-7 cells stained with AAA19984 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C18orf22/RBFA Antibody (AAA19984, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of C18orf22/RBFA using anti-C18orf22/RBFA antibody (AAA19984).C18orf22/RBFA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-C18orf22/RBFA Antibody (AAA19984) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MOLT-4 whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C18orf22/RBFA antigen affinity purified polyclonal antibody (#AAA19984) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C18orf22/RBFA at approximately 40 kDa. The expected band size for C18orf22/RBFA is at 38 kDa.)

C18orf22/RBFA, Polyclonal Antibody (Cat# AAA19984)

Full Name

Anti-C18orf22/RBFA Antibody Picoband

Gene Names

RBFA; HsT169; C18orf22

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U87 cells using anti-IFIT5 antibody (AAA19582).Overlay histogram showing U87 cells stained with AAA19582 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IFIT5 Antibody (AAA19582, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IFIT5, Polyclonal Antibody (Cat# AAA19582)

Full Name

Anti-IFIT5 Antibody Picoband

Gene Names

IFIT5; P58; RI58

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of SiHa cells using anti-MLST8 antibody (AAA19842).Overlay histogram showing SiHa cells stained with AAA19842 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MLST8 Antibody (AAA19842, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of MLST8 using anti-MLST8 antibody (AAA19842) and anti-Beta Tubulin antibody (M01857-3).MLST8 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLST8 Antibody (AAA19842) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLST8 Antibody (AAA19842) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLST8 Antibody (AAA19842) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLST8 Antibody (AAA19842) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MLST8 Antibody (AAA19842) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat kidney tissue lysates,Lane 3: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLST8 antigen affinity purified polyclonal antibody (#AAA19842) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLST8 at approximately 36 kDa. The expected band size for MLST8 is at 36 kDa.)

MLST8, Polyclonal Antibody (Cat# AAA19842)

Full Name

Anti-MLST8 Antibody Picoband

Gene Names

MLST8; GBL; LST8; POP3; WAT1; GbetaL

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-GIPC1 antibody (AAA19828).Overlay histogram showing HepG2 cells stained with AAA19828 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GIPC1 Antibody (AAA19828, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of GIPC1 using anti-GIPC1 antibody (AAA19828).GIPC1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GIPC1 Antibody (AAA19828) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: rat skeletal muscle tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: rat NRK whole cell lysates,Lane 6: mouse skeletal muscle tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (#AAA19828) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.)

GIPC1, Polyclonal Antibody (Cat# AAA19828)

Full Name

Anti-GIPC1 Antibody Picoband

Gene Names

GIPC1; NIP; GIPC; IIP-1; TIP-2; SEMCAP; C19orf3; Hs.6454; GLUT1CBP; RGS19IP1; SYNECTIN; SYNECTIIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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SCO1, Polyclonal Antibody (Cat# AAA19474)

Full Name

Anti-SCO1 Antibody Picoband

Gene Names

SCO1; SCOD1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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NDUFAF1, Polyclonal Antibody (Cat# AAA19616)

Full Name

Anti-NDUFAF1 Antibody Picoband

Gene Names

NDUFAF1; CGI65; CIA30; CGI-65

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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MTHFD1, Polyclonal Antibody (Cat# AAA19442)

Full Name

Anti-MTHFD1 Antibody Picoband

Gene Names

MTHFD1; MTHFC; MTHFD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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GNB3, Polyclonal Antibody (Cat# AAA19456)

Full Name

Anti-GNB3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody at dilution of 1:100 (40x lens).)

CETN3, Polyclonal Antibody (Cat# AAA28193)

Full Name

CETN3 Polyclonal Antibody

Gene Names

CETN3; CEN3; CDC31

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged DDX56 is 0.1 ng/ml as a capture antibody.)

DDX56, Monoclonal Antibody (Cat# AAA26253)

Full Name

DDX56 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 56, DDX21, DDX26, NOH61) (Biotin)

Gene Names

DDX56; DDX21; DDX26; NOH61

Applications

Western Blot

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RPL19 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

RPL19, Polyclonal Antibody (Cat# AAA28389)

Full Name

RPL19 Rabbit pAb

Gene Names

RPL19; L19

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometry analysis of Hsp105alpha in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)

HSP105alpha, Monoclonal Antibody (Cat# AAA11727)

Full Name

HSP105alpha antibody

Gene Names

HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry

Purity

By protein-G affinity chromatography

Pricing

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IP (Immunoprecipitation) (Figure 7. Immunoprecipitating IDH3B in U251 whole cell lysate.Western blot analysis of IDH3B using anti-IDH3B antibody (AAA19926).Lane 1: U251 whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-IDH3B antibody in U251 whole cell lysate.Lane 3: anti-IDH3B antibody (2ug) + U251 whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IDH3B antigen affinity purified polyclonal antibody (AAA19926) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH3B Antibody (AAA19926, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of IDH3B using anti-IDH3B antibody (AAA19926).IDH3B was detected in a paraffin-embedded section of follicles of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3B Antibody (AAA19926) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3B Antibody (AAA19926) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3B Antibody (AAA19926) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IDH3B Antibody (AAA19926) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human U251 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat heart tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH3B antigen affinity purified polyclonal antibody (#AAA19926) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IDH3B at approximately 42 kDa. The expected band size for IDH3B is at 42 kDa.)

IDH3B, Polyclonal Antibody (Cat# AAA19926)

Full Name

Anti-IDH3B Antibody Picoband

Gene Names

IDH3B; RP46; H-IDHB

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-LARG/ARHGEF12 antibody (AAA19877).Overlay histogram showing HepG2 cells stained with AAA19877 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LARG/ARHGEF12 Antibody (AAA19877, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of ARHGEF12 using anti-ARHGEF12 antibody (AAA19877) and anti-Beta Tubulin antibody (M01857-3).ARHGEF12 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ARHGEF12L Antibody (AAA19877) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat testis tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LARG/ARHGEF12 antigen affinity purified polyclonal antibody (#AAA19877) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LARG/ARHGEF12 at approximately 220 kDa. The expected band size for LARG/ARHGEF12 is at 173 kDa.)

LARG/ARHGEF12, Polyclonal Antibody (Cat# AAA19877)

Full Name

Anti-LARG/ARHGEF12 Antibody Picoband

Gene Names

ARHGEF12; LARG; PRO2792

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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TTC7B, Polyclonal Antibody (Cat# AAA19626)

Full Name

Anti-TTC7B Antibody Picoband

Gene Names

TTC7B; TTC7L1; c14_5685

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

SCN3B, Polyclonal Antibody (Cat# AAA28227)

Full Name

SCN3B Polyclonal Antibody

Gene Names

SCN3B; SCNB3; ATFB16; BRGDA7; HSA243396

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity Purification

Pricing

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Application Data (Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)

DNAJB6, Monoclonal Antibody (Cat# AAA25669)

Full Name

DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) (PE)

Gene Names

DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of MAP6D1 using anti-MAP6D1 antibody (AAA20004).MAP6D1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP6D1 Antibody (AAA20004, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MAP6D1 using anti-MAP6D1 antibody (AAA20004).MAP6D1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MAP6D1 Antibody (AAA20004) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP6D1 antigen affinity purified polyclonal antibody (#AAA20004) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP6D1 at approximately 21 kDa. The expected band size for MAP6D1 is at 21 kDa.)

MAP6D1, Polyclonal Antibody (Cat# AAA20004)

Full Name

Anti-MAP6D1 Antibody Picoband

Gene Names

MAP6D1; SL21; MAPO6D1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

DNAJC10, Monoclonal Antibody (Cat# AAA25670)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (PE)

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEL cells using anti-STXBP6 antibody (AAA19956).Overlay histogram showing HEL cells stained with AAA19956 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP6 Antibody (AAA19956, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of STXBP6 using anti-STXBP6 antibody (AAA19956).STXBP6 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STXBP6 Antibody (AAA19956) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human Ramos whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP6 antigen affinity purified polyclonal antibody (#AAA19956) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STXBP6 at approximately 26 kDa. The expected band size for STXBP6 is at 24 kDa.)

STXBP6, Polyclonal Antibody (Cat# AAA19956)

Full Name

Anti-STXBP6 Antibody Picoband

Gene Names

STXBP6; amisyn; HSPC156

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A549 cells using anti-SLK antibody (AAA19754).Overlay histogram showing A549 cells stained with AAA19754 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLK Antibody (AAA19754, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SLK using anti-SLK antibody (AAA19754).SLK was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SLK Antibody (AAA19754) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A431 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLK antigen affinity purified polyclonal antibody (#AAA19754) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLK at approximately 245 kDa. The expected band size for SLK is at 143 kDa.)

SLK, Polyclonal Antibody (Cat# AAA19754)

Full Name

Anti-SLK Antibody Picoband

Gene Names

SLK; LOSK; STK2; se20-9; bA16H23.1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-MCM7 antibody (AAA19689).Overlay histogram showing MCF-7 cells stained with AAA19689 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-MCM7 Antibody (AAA19689, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MCM7, Monoclonal Antibody (Cat# AAA19689)

Full Name

Anti-MCM7 Antibody Picoband (monoclonal, 3H11)

Gene Names

MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; P1.1-MCM3

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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TTLL2, Polyclonal Antibody (Cat# AAA19641)

Full Name

Anti-TTLL2 Antibody Picoband

Gene Names

TTLL2; C6orf104; NYD-TSPG; dJ366N23.3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (LDB3 monoclonal antibody Western Blot analysis of LDB3 expression in A-431.)

LDB3, Monoclonal Antibody (Cat# AAA25437)

Full Name

LDB3 (LIM Domain-binding Protein 3, Protein Cypher, Z-band Alternatively Spliced PDZ-motif Protein, KIAA0613, ZASP) (HRP)

Gene Names

LDB3; MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-RPL18 antibody (AAA19881).Overlay histogram showing Hela cells stained with AAA19881 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL18 Antibody (AAA19881, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RPL18 using anti-RPL18 antibody (AAA19881).RPL18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPL18 Antibody (AAA19881) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human Daudi whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL18 antigen affinity purified polyclonal antibody (#AAA19881) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPL18 at approximately 23 kDa. The expected band size for RPL18 is at 22 kDa.)

RPL18, Polyclonal Antibody (Cat# AAA19881)

Full Name

Anti-RPL18 Antibody Picoband

Gene Names

RPL18; L18

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of OPA1 expression in transfected 293T cell line by OPA1 monoclonal antibody (M06), clone 8A32.Lane 1: OPA1 transfected lysate (Predicted MW: 111.6 KDa).Lane 2: Non-transfected lysate.)

OPA1, Monoclonal Antibody (Cat# AAA26419)

Full Name

OPA1 (Pptic Atrophy 1 (Autosomal Dominant), FLJ12460, KIAA0567, MGM1, NPG, NTG, LargeG) (FITC)

Gene Names

OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14

Applications

Western Blot

Purity

Purified

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of JK cells using anti-GSPT1,2 antibody (AAA19586).Overlay histogram showing JK cells stained with AAA19586 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GSPT1,2 Antibody (AAA19586, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

GSPT1,2, Polyclonal Antibody (Cat# AAA19586)

Full Name

Anti-GSPT1,2 Antibody Picoband

Gene Names

GSPT1; GST1; ETF3A; eRF3a; 551G9.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti-WDR44 antibody (AAA19624).Overlay histogram showing SiHa cells stained with AAA19624 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR44 Antibody (AAA19624, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WDR44, Polyclonal Antibody (Cat# AAA19624)

Full Name

Anti-WDR44 Antibody Picoband

Gene Names

WDR44; RPH11; RAB11BP

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using DOK1 Antibody at dilution of 1:100 (40x lens).)

DOK1, Polyclonal Antibody (Cat# AAA28181)

Full Name

DOK1 Polyclonal Antibody

Gene Names

DOK1; P62DOK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

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SMARCE1, Polyclonal Antibody (Cat# AAA28107)

Full Name

SMARCE1 Polyclonal Antibody

Gene Names

SMARCE1; BAF57

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity Purification

Pricing

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MCM6, Monoclonal Antibody (Cat# AAA19372)

Full Name

Anti-MCM6 Antibody (monoclonal, 10I9)

Gene Names

MCM6; Mis5; P105MCM; MCG40308

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Hela cells using anti-PACSIN2 antibody (AAA19809).Overlay histogram showing Hela cells stained with AAA19809 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PACSIN2 Antibody (AAA19809, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PACSIN2 using anti-PACSIN2 antibody (AAA19809).PACSIN2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PACSIN2 Antibody (AAA19809) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: monkey COS-7 whole cell lysates,Lane 3: human placenta tissue lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: rat lung tissue lysates,Lane 6: rat heart tissue tissue lysates,Lane 7: mouse lung tissue lysates,Lane 8: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACSIN2 antigen affinity purified polyclonal antibody (#AAA19809) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (60-65 kDa.)

PACSIN2, Polyclonal Antibody (Cat# AAA19809)

Full Name

Anti-PACSIN2 Antibody Picoband

Gene Names

PACSIN2; SDPII

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

IHC (Immunohistochemistry) (Figure 8. IHC analysis of RPL29 using anti-RPL29 antibody (AAA19561).RPL29 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RPL29 Antibody (AAA19561) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

RPL29, Polyclonal Antibody (Cat# AAA19561)

Full Name

Anti-RPL29 Antibody Picoband

Gene Names

RPL29; HIP; L29; HUMRPL29; RPL29P10; RPL29_3_370

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of RT4 cells using anti-RFX3 antibody (AAA19892).Overlay histogram showing RT4 cells stained with AAA19892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFX3 Antibody (AAA19892, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of RFX3 using anti-RFX3 antibody (AAA19892) and anti-Tubulin Alpha antibody (M03989-3).RFX3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX3 Antibody (AAA19892) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RFX3 Antibody (AAA19892) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SH-SY5Y whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX3 antigen affinity purified polyclonal antibody (#AAA19892) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RFX3 at approximately 95 kDa. The expected band size for RFX3 is at 84 kDa.)

RFX3, Polyclonal Antibody (Cat# AAA19892)

Full Name

Anti-RFX3 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

< >

GNG11, Polyclonal Antibody (Cat# AAA19638)

Full Name

Anti-GNG11 Antibody Picoband

Gene Names

GNG11; GNGT11

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-PYCR1 antibody (AAA19858).Overlay histogram showing 293T cells stained with AAA19858 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYCR1 Antibody (AAA19858, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PYCR1, Polyclonal Antibody (Cat# AAA19858)

Full Name

Anti-PYCR1 Antibody Picoband

Gene Names

PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Jurkat cells using anti-SUCLG1 antibody (AAA19867).Overlay histogram showing Jurkat cells stained with AAA19867 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLG1 Antibody (AAA19867, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of SUCLG1 using anti-SUCLG1 antibody (AAA19867).SUCLG1 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG1 Antibody (AAA19867) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG1 antigen affinity purified polyclonal antibody (#AAA19867) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SUCLG1 at approximately 35 kDa. The expected band size for SUCLG1 is at 36 kDa.)

SUCLG1, Polyclonal Antibody (Cat# AAA19867)

Full Name

Anti-SUCLG1 Antibody Picoband

Gene Names

SUCLG1; GALPHA; MTDPS9; SUCLA1

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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SCML1, Polyclonal Antibody (Cat# AAA19625)

Full Name

Anti-SCML1 Antibody Picoband

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of 293T cells using anti-NCOA5 antibody (AAA19894).Overlay histogram showing 293T cells stained with AAA19894 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCOA5 Antibody (AAA19894, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of NCOA5 using anti-NCOA5 antibody (AAA19894) and anti-Beta Tubulin antibody (M01857-3).NCOA5 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NCOA5 Antibody (AAA19894) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NCOA5 Antibody (AAA19894) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NCOA5 Antibody (AAA19894) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NCOA5 Antibody (AAA19894) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NCOA5 Antibody (AAA19894) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA5 antigen affinity purified polyclonal antibody (#AAA19894) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NCOA5 at approximately 66 kDa. The expected band size for NCOA5 is at 66 kDa.)

NCOA5, Polyclonal Antibody (Cat# AAA19894)

Full Name

Anti-NCOA5 Antibody Picoband

Gene Names

NCOA5; CIA; bA465L10.6

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

IHC (Immunohistochemistry) (Figure 10. IHC analysis of ISYNA1 using anti-ISYNA1 antibody (AAA19907).ISYNA1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ISYNA1 Antibody (AAA19907) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HEL whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: mouse testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISYNA1 antigen affinity purified polyclonal antibody (#AAA19907) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ISYNA1 at approximately 61 kDa. The expected band size for ISYNA1 is at 61 kDa.)

ISYNA1, Polyclonal Antibody (Cat# AAA19907)

Full Name

Anti-ISYNA1 Antibody Picoband

Gene Names

ISYNA1; IPS; INO1; INOS; IPS 1; IPS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-PYHIN1 antibody (AAA19944).Overlay histogram showing JK cells stained with AAA19944 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PYHIN1 Antibody (AAA19944, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PYHIN1 using anti-PYHIN1 antibody (AAA19944).PYHIN1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PYHIN1 Antibody (AAA19944) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYHIN1 antigen affinity purified polyclonal antibody (#AAA19944) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PYHIN1 at approximately 55 kDa. The expected band size for PYHIN1 is at 55 kDa.)

PYHIN1, Polyclonal Antibody (Cat# AAA19944)

Full Name

Anti-PYHIN1 Antibody Picoband

Gene Names

PYHIN1; IFIX

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-ORC2 antibody (AAA19812).Overlay histogram showing HepG2 cells stained with AAA19812 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ORC2 Antibody (AAA19812, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of ORC2 using anti-ORC2 antibody (AAA19812) and anti-Beta Tubulin antibody (M01857-3).ORC2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ORC2 Antibody (AAA19812) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ORC2 Antibody (AAA19812) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ORC2 Antibody (AAA19812) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ORC2 Antibody (AAA19812) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human U20S whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC2 antigen affinity purified polyclonal antibody (#AAA19812) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human A2780 whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC2 antigen affinity purified polyclonal antibody (#AAA19812) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ORC2 at approximately 74 kDa. The expected band size for ORC2 is at 66 kDa.)

ORC2, Polyclonal Antibody (Cat# AAA19812)

Full Name

Anti-ORC2 Antibody Picoband

Gene Names

ORC2; ORC2L

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-CEP250 antibody (AAA19580).Overlay histogram showing SiHa cells stained with AAA19580 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEP250 Antibody (AAA19580, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CEP250, Polyclonal Antibody (Cat# AAA19580)

Full Name

Anti-CEP250 Antibody Picoband

Gene Names

CEP250; CEP2; CNAP1; C-NAP1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

Hypoxia-Inducible Factor-1 Alpha, Recombinant Protein (Cat# AAA10841)

Full Name

Recombinant Human Hypoxia-Inducible Factor-1 Alpha

Gene Names

HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA

Purity

Greater than 90.0% as determined by SDS-PAGE.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of MED15 using anti-MED15 antibody (AAA19169).MED15 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED15 Antibody (AAA19169) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MED15/Pcqap, Polyclonal Antibody (Cat# AAA19169)

Full Name

Anti-MED15/Pcqap Picoband Antibody

Gene Names

MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified

Pricing

CD86, ELISA Kit (Cat# AAA17898)

Full Name

Mouse CD86 ELISA Kit

Gene Names

Cd86; B7; B70; MB7; B7-2; B7.2; CLS1; Ly58; ETC-1; Ly-58; MB7-2; Cd28l2; TS/A-2

Reactivity

Mouse

Applications

Sandwich ELISA

Pricing

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10000 results for "Recombinant Proteins" - showing 1650-1700

PRMT8, Polyclonal Antibody (Cat# AAA19321)

MRPS25, Polyclonal Antibody (Cat# AAA19639)

NOLA1/GAR1, Polyclonal Antibody (Cat# AAA19579)

BCKDHA, Polyclonal Antibody (Cat# AAA19523)

C18orf22/RBFA, Polyclonal Antibody (Cat# AAA19984)

IFIT5, Polyclonal Antibody (Cat# AAA19582)

MLST8, Polyclonal Antibody (Cat# AAA19842)

GIPC1, Polyclonal Antibody (Cat# AAA19828)

SCO1, Polyclonal Antibody (Cat# AAA19474)

NDUFAF1, Polyclonal Antibody (Cat# AAA19616)

MTHFD1, Polyclonal Antibody (Cat# AAA19442)

GNB3, Polyclonal Antibody (Cat# AAA19456)

CETN3, Polyclonal Antibody (Cat# AAA28193)

DDX56, Monoclonal Antibody (Cat# AAA26253)

RPL19, Polyclonal Antibody (Cat# AAA28389)

HSP105alpha, Monoclonal Antibody (Cat# AAA11727)

IDH3B, Polyclonal Antibody (Cat# AAA19926)

LARG/ARHGEF12, Polyclonal Antibody (Cat# AAA19877)

TTC7B, Polyclonal Antibody (Cat# AAA19626)

SCN3B, Polyclonal Antibody (Cat# AAA28227)

DNAJB6, Monoclonal Antibody (Cat# AAA25669)

MAP6D1, Polyclonal Antibody (Cat# AAA20004)

DNAJC10, Monoclonal Antibody (Cat# AAA25670)

STXBP6, Polyclonal Antibody (Cat# AAA19956)

SLK, Polyclonal Antibody (Cat# AAA19754)

MCM7, Monoclonal Antibody (Cat# AAA19689)

TTLL2, Polyclonal Antibody (Cat# AAA19641)

LDB3, Monoclonal Antibody (Cat# AAA25437)

RPL18, Polyclonal Antibody (Cat# AAA19881)

OPA1, Monoclonal Antibody (Cat# AAA26419)

GSPT1,2, Polyclonal Antibody (Cat# AAA19586)

WDR44, Polyclonal Antibody (Cat# AAA19624)

DOK1, Polyclonal Antibody (Cat# AAA28181)

SMARCE1, Polyclonal Antibody (Cat# AAA28107)

MCM6, Monoclonal Antibody (Cat# AAA19372)

PACSIN2, Polyclonal Antibody (Cat# AAA19809)

RPL29, Polyclonal Antibody (Cat# AAA19561)

RFX3, Polyclonal Antibody (Cat# AAA19892)

GNG11, Polyclonal Antibody (Cat# AAA19638)

PYCR1, Polyclonal Antibody (Cat# AAA19858)

SUCLG1, Polyclonal Antibody (Cat# AAA19867)

SCML1, Polyclonal Antibody (Cat# AAA19625)

NCOA5, Polyclonal Antibody (Cat# AAA19894)

ISYNA1, Polyclonal Antibody (Cat# AAA19907)

PYHIN1, Polyclonal Antibody (Cat# AAA19944)

ORC2, Polyclonal Antibody (Cat# AAA19812)

CEP250, Polyclonal Antibody (Cat# AAA19580)

Hypoxia-Inducible Factor-1 Alpha, Recombinant Protein (Cat# AAA10841)

MED15/Pcqap, Polyclonal Antibody (Cat# AAA19169)

CD86, ELISA Kit (Cat# AAA17898)

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