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10000 results for "Recombinant Proteins" - showing 4800-4850
SDS-PAGE
(SDS-PAGE)
Platelet Derived Growth Factor D (PDGFD), Recombinant Protein (Cat# AAA152059)
Full Name
Recombinant Platelet Derived Growth Factor D (PDGFD)
Gene Names
PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
ELISA
(Biotinylated Human ALCAM/CD166 Protein ELISA DataImmobilized Biotinylated Human ALCAM, His Tag at 2?g/ml (100?l/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.48?g/ml determined by ELISA.)
ALCAM/CD166, Recombinant Protein (Cat# AAA76155)
Full Name
Biotinylated Human ALCAM/CD166 Protein Recombinant
Gene Names
ALCAM; MEMD; CD166
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
Amino Acid Info
(Amino Acid info)
Chemokine C-X-C-Motif Receptor 5 (CXCR5), Recombinant Protein (Cat# AAA153407)
Full Name
Recombinant Chemokine C-X-C-Motif Receptor 5 (CXCR5)
Gene Names
CXCR5; BLR1; CD185; MDR15
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
Amino Acid Info
(Amino Acid info)
Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked (aTRX), Recombinant Protein (Cat# AAA153695)
Full Name
Recombinant Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked (aTRX)
Gene Names
ATRX; JMS; SHS; XH2; XNP; ATR2; SFM1; RAD54; MRXHF1; RAD54L; ZNF-HX
Applications
Western Blot
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(SDS-PAGE)
Chemokine C-X-C-Motif Ligand 16 (CXCL16), Recombinant Protein (Cat# AAA152094)
Full Name
Recombinant Chemokine C-X-C-Motif Ligand 16 (CXCL16)
Gene Names
Cxcl16; SR-PSOX; Zmynd15; AV290116; BB024863; CXCL16v1; CXCL16v2; b2b498Clo; 0910001K24Rik
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IHC (Immunohistochemistry)
(Anti- HSP27 antibody, AAA46032, IHC(F)IHC(F): Human Placenta Tissue)
Heat shock protein beta-1, Polyclonal Antibody (Cat# AAA46032)
Full Name
Anti-Hsp27 Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human. No cross reactivity with other proteins.
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
SDS-PAGE
Versican, Recombinant Protein (Cat# AAA144048)
Full Name
Recombinant Versican (VCAN)
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 95%
Pricing
SDS-PAGE
Solute Carrier Family 39, Recombinant Protein (Cat# AAA143968)
Full Name
Recombinant Solute Carrier Family 39, Member 6 (SLC39A6)
Applications
Western Blot
Purity
> 97%
Pricing
IHC (Immunohistochemisry)
(Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine(DAB).)
STEAP1, Monoclonal Antibody (Cat# AAA48032)
Full Name
STEAP1 antibody
Gene Names
STEAP1; STEAP; PRSS24
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
By protein-G affinity chromatography
Pricing
ELISA
(Biotinylated Human Siglec-10 (R119A) Protein ELISA DataImmobilized Anti-Siglec-10 Antibody, hFc Tag at 5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 61.7ng/ml determined by ELISA.)
Siglec-10 (R119A), Recombinant Protein (Cat# AAA76352)
Full Name
Biotinylated Human Siglec-10 (R119A) Protein Recombinant
Gene Names
SIGLEC10; SLG2; PRO940; SIGLEC-10
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
SDS-PAGE
Heat Shock Transcription Factor 1 (HSF1), Recombinant Protein (Cat# AAA135485)
Full Name
Recombinant Heat Shock Transcription Factor 1 (HSF1)
Gene Names
HSF1; HSTF1
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 90%
Pricing
IP (Immunoprecipitation)
(ADSL was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-ADSL rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ADSL rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 50 kDaObserved band size: 50 kDa)
Adenylosuccinate Lyase, Monoclonal Antibody (Cat# AAA255391)
Full Name
Recombinant Anti-Adenylosuccinate Lyase Antibody, Rabbit Monoclonal
Gene Names
ADSL; ASL; AMPS; ASASE
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of ARG1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ARG1 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).)
Arginase, Monoclonal Antibody (Cat# AAA255426)
Full Name
Recombinant Anti-Arginase Antibody, Rabbit Monoclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Protein A
Pricing
IP (Immunoprecipitation)
(LTA4H was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-LTA4H rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
Leukotriene A4 Hydrolase, Monoclonal Antibody (Cat# AAA254782)
Full Name
Recombinant Anti-Leukotriene A4 Hydrolase Antibody, Rabbit Monoclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
SDS-PAGE
Intercellular Adhesion Molecule 5, Recombinant Protein (Cat# AAA143934)
Full Name
Recombinant Intercellular Adhesion Molecule 5 (ICAM5)
Gene Names
Icam5; TLN; CD50; Tlcn; Icam3; ICAM-3
Reactivity
Mus musculus (Mouse)
Applications
Western Blot
Purity
> 95%
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified CD71 Rabbit Recombinant Monoclonal Antibody (TFRC/2898R). Confirmation of Purity and Integrity of Antibody.)
CD71/Transferrin Receptor (TFRC), Antibody (Cat# AAA215217)
Full Name
CD71/Transferrin Receptor (TFRC) (Extracellular Domain)
Gene Names
TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
WB (Western Blot)
(Anti-CD74 rabbit polyclonal antibody at 1:500 dilutionLane A: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:34 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
CD74, Polyclonal Antibody (Cat# AAA255320)
Full Name
Anti-CD74 Antibody, Rabbit Polyclonal
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
SDS-PAGE
Platelet Derived Growth Factor Receptor Beta, Recombinant Protein (Cat# AAA144283)
Full Name
Recombinant Platelet Derived Growth Factor Receptor Beta (PDGFRb)
Gene Names
Pdgfrb; PDGFR-1
Reactivity
Mus musculus (Mouse)
Applications
Western Blot
Purity
> 97%
Pricing
WB (Western Blot)
(Western Blot; Sample: Lane1: Human Serum; Lane2: Human Plasma; Lane2: Human Placenta lysate Primary Ab: 0.04ug/ml Mouse AntiHuman ANG Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Angiostatin (ANG), Monoclonal Antibody (Cat# AAA151697)
Full Name
Monoclonal Antibody to Angiostatin (ANG)
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of AK3 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
AK3, Monoclonal Antibody (Cat# AAA47983)
Full Name
AK3 antibody
Gene Names
AK3; AK6; FIX; AK3L1; AKL3L; AKL3L1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
By protein-G affinity chromatography
Pricing
WB (Western Blot)
(Western Blot; Sample: Human Serum Primary Ab: 3ug/ml Mouse AntiHuman Ceruloplasmin Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Ceruloplasmin (CP), Monoclonal Antibody (Cat# AAA151654)
Full Name
Monoclonal Antibody to Ceruloplasmin (CP)
Gene Names
CP; CP-2
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
ELISA
(Biotinylated Human 4-1BB/TNFRSF9 Protein ELISA DataImmobilized Human 4-1BB Ligand Trimer, hFc Tag at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human 4-1BB, His Tag with the EC50 of 0.58?g/ml determined by ELISA.)
4-1BB/TNFRSF9, Recombinant Protein (Cat# AAA76198)
Full Name
Biotinylated Human 4-1BB/TNFRSF9 Protein Recombinant
Gene Names
TNFRSF9; ILA; 4-1BB; CD137; CDw137
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
SDS-PAGE
(SDS-PAGE)
Cluster of Differentiation 59 (CD59), Recombinant Protein (Cat# AAA152114)
Full Name
Eukaryotic Cluster of Differentiation 59 (CD59)
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.1 (K18I) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads in 400 ul binding buffer containing 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1hours at 4?. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min. at 9 degree C. 2.4 ul samples were loaded and run on a 12.5% SDSPAGE gel and stained by Coommassie Blue. The SDS-PAGE gel result showed that 80% of biotinylated mononucleosomes were pulled down by streptavidin beads. * indicates streptavidin.)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60195)
Full Name
Recombinant Mononucleosomes H3.1 (K18I)-biotin
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (AAA124715).Kallikrein 2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Kallikrein 2 Antibody (AAA124715) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Kallikrein 2, Polyclonal Antibody (Cat# AAA124715)
Full Name
Anti-Kallikrein 2 Picoband antibody
Gene Names
KLK2; hK2; hGK-1; KLK2A2
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
Bioactivity
(C4BPa, also known as C4b-binding protein alpha chain or C4BPalpha, is a subunit of the complement factor C4b-binding protein (C4BP), which plays a crucial role in the regulation of the complement system, a part of the immune system that helps to defend the body against infection.C4BPa is part of a heterodimeric protein complex that consists of two chains, the alpha chain (C4BPa) and the beta chain (C4BPB). It can bind to C4b and C3b, thereby inhibiting the formation of the C3 convertase and preventing excessive complement activation. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C4BPa and recombinant mouse C4b. Briefly, C4BPa was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C4b-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C4BPa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C4BPa and recombinant mouse C4b was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL)
C4 Binding Protein Alpha (C4BPa), Active Protein (Cat# AAA161854)
Full Name
Active C4 Binding Protein Alpha (C4BPa)
Gene Names
C4BPA; PRP; C4BP
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-SMOC1 antibody (AAA127472).Overlay histogram showing HepG2 cells stained with AAA127472 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SMOC1 Antibody (AAA127472, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SMOC1, Polyclonal Antibody (Cat# AAA127472)
Full Name
Anti-SMOC1 Antibody Picoband
Gene Names
SMOC1; OAS
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
ICC (Immunocytochemistry)
(Immunocytochemistry stain of Hela using Mannose Phosphate Isomerase mouse mAb (1:300).)
Mannose Phosphate Isomerase, Monoclonal Antibody (Cat# AAA320310)
Full Name
Mannose Phosphate Isomerase Mouse mAb
Gene Names
MPI; PMI; PMI1; CDG1B
Reactivity
Human, Rat
Applications
Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using CD14 Mouse Monoclonal Antibody (LPSR/2385) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
CD14, Monoclonal Antibody (Cat# AAA214581)
Full Name
CD14 (Monocyte/Macrophage Marker)
Reactivity
Human. Others not tested.
Applications
Immunohistochemistry
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using MCAM Mouse Monoclonal Antibody (MCAM/3046). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
MCAM (Melanoma Cell Adhesion Molecule)/MUC18/CD146, Monoclonal Antibody (Cat# AAA215077)
Full Name
MCAM (Melanoma Cell Adhesion Molecule)/MUC18/CD146
Gene Names
MCAM; CD146; MUC18; HEMCAM; METCAM; MelCAM
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Napsin A Mouse Monoclonal Antibody (NAPSA/3308). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
Napsin A, Monoclonal Antibody (Cat# AAA215301)
Full Name
Napsin A (Lung Adenocarcinoma Marker)
Gene Names
NAPSA; KAP; Kdap; NAP1; NAPA; SNAPA
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of RXRA using anti-RXRA antibody (AAA124639).RXRA was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RXRA Antibody (AAA124639) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RXRA/Rxr Alpha, Polyclonal Antibody (Cat# AAA124639)
Full Name
Anti-RXRA/Rxr Alpha Picoband antibody
Gene Names
RXRA; NR2B1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 30ug/ml Mouse AntiHuman CHEM AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Chemerin (CHEM), Monoclonal Antibody (Cat# AAA151661)
Full Name
Monoclonal Antibody to Chemerin (CHEM)
Gene Names
RARRES2; TIG2; HP10433
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
IHC (Immunohiostchemistry)
(DAB staining on IHCP;Sample: Human Skeletal muscle Tissue; Primary Ab: 20ug/ml Mouse AntiHuman ISLR AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR), Monoclonal Antibody (Cat# AAA151880)
Full Name
Monoclonal Antibody to Immunoglobulin Superfamily Containing Leucine Rich Repeat Protein (ISLR)
Gene Names
ISLR; HsT17563
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using EZH2 Mouse Monoclonal Antibody (EZH2/2536). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
EZH2/KMT6, Monoclonal Antibody (Cat# AAA214964)
Full Name
EZH2/KMT6
Gene Names
EZH2; WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human
Applications
Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U87 cells using anti-Wnt5b antibody (AAA125761).Overlay histogram showing U87 cells stained with AAA125761 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Wnt5b Antibody (AAA125761,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Wnt5b, Polyclonal Antibody (Cat# AAA125761)
Full Name
Anti-Wnt5b Antibody
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(IL-33 was immunoprecipitated using:Lane A:0.5 mg HeLa, K562, jurkat Whole Cell Lysate2 uL anti-IL-33 mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-IL-33 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size :25 kDa)
IL-33, Monoclonal Antibody (Cat# AAA258795)
Full Name
Anti-IL-33 Antibody, Mouse Monoclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A
Pricing
Bioactivity
(Endothelial protein C receptor (EPCR) also known as CD201, is a transmembrane glycoprotein expressed on vascular endothelial cells and functions as a negative regulator of thrombosis. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Mature human EPCR consists of a 193 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 7 aa cytoplasmic tail. Within the ECD, human EPCR shares 63% and 66% aa sequence identity with mouse and rat EPCR, respectively. EPCR inhibits thrombosis through its interactions with Protein C, activated Protein C (APC), and Coagulation Factors VII, and VIIa. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EPCR and recombinant rat Coagulation Factor VII (F7). Briefly, EPCR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F7-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EPCR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human EPCR and recombinant rat F7 was shown in Figure 1, the EC50 for this effect is 0.06 ug/mL.)
Endothelial protein C receptor (EPCR), Active Protein (Cat# AAA161658)
Full Name
Active Endothelial protein C receptor (EPCR)
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using CD68 Mouse Monoclonal Antibody (C68 /2511). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
CD68, Monoclonal Antibody (Cat# AAA215315)
Full Name
CD68 (Macrophage Marker)
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
WB (Western Blot)
(Western Blot: The extract of lung (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MFAP4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
MFAP4, Monoclonal Antibody (Cat# AAA52584)
Full Name
Anti-MFAP4 Antibody (clone AT12D11) IHC-plus
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A purified
Pricing
WB (Western Blot)
(Figure 2. Western blot analysis of ALPL using anti-ALPL antibody (AAA125583).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HEK293 whole cell lysatesLane 3: rat kidney tissue lysatesLane 4: rat RH-35 whole cell lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse HEPA1-6 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ALPL antigen affinity purified polyclonal antibody (Catalog # AAA125583) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALPL at approximately 80KD. The expected band size for ALPL is at 57KD.)
Alkaline phosphatase/ALPL, Polyclonal Antibody (Cat# AAA125583)
Full Name
Anti-Alkaline phosphatase/ALPL Antibody
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using PAPP-A Mouse Monoclonal Antibody (PAPPA/2718) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
PAPP-A/Pappalysin-1, Monoclonal Antibody (Cat# AAA215124)
Full Name
PAPP-A/Pappalysin-1 (Marker of Atherosclerosis and Aneuploid Fetus)
Gene Names
PAPPA; PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Dystrophin Monospecific Mouse Monoclonal Antibody (DMD/3243). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
Dystrophin (DMD), Monoclonal Antibody (Cat# AAA214924)
Full Name
Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy)
Gene Names
DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of K562 cells using anti-C-Kit antibody (AAA46218).Overlay histogram showing K562 cells stained with AAA46218 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Kit Antibody (AAA46218,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
c-Kit, Polyclonal Antibody (Cat# AAA46218)
Full Name
Anti-c-Kit Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ZAP70 Mouse Monoclonal Antibody (ZAP70/2047). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ZAP70, Monoclonal Antibody (Cat# AAA214835)
Full Name
ZAP70 (Chronic Lymphocytic Leukemia Marker)
Gene Names
ZAP70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70
Reactivity
Human. Others not known
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
WB (Western Blot)
(Western Blot Sample: Lane1: Human Placenta lysate; Lane2: Human Urine Primary Ab: 2ug/ml Mouse AntiHuman APOH Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Apolipoprotein H (APOH), Monoclonal Antibody (Cat# AAA151522)
Full Name
Monoclonal Antibody to Apolipoprotein H (APOH)
Gene Names
APOH; BG; B2G1; B2GP1
Reactivity
Human, Pig
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHCP;Sample: Human Kidney Tissue; Primary Ab: 30ug/ml Mouse AntiHuman VEGF121 AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Vascular Endothelial Growth Factor 121 (VEGF121), Monoclonal Antibody (Cat# AAA151759)
Full Name
Monoclonal Antibody to Vascular Endothelial Growth Factor 121 (VEGF121)
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
WB (Western Blot)
(Western Blot; Sample: Rat Spleen lysate; Primary Ab: 3ug/ml Mouse AntiHuman KIR2DS4 AntibodySecond Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))
Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4), Monoclonal Antibody (Cat# AAA151728)
Full Name
Monoclonal Antibody to Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4)
Gene Names
KIR2DS4; KKA3; KIR1D; NKAT8; CD158I; KIR412; NKAT-8; KIR2DS1
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Pricing
Standard Curve (Sample)
(The titer has been suggested in reference to an "in-house" ELISA kit performed at Rekom Biotech over the first lot obtained.Each end user should carry out their own titration for their particular application.Figure 2. In this plot, the optical density at 450/620 nm for positive (blue) and negative (gray) IgM sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the pvalue at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefore, any of the showed concentrations can be used to distinguish between positive and negative sera.)
p18, Recombinant Protein (Cat# AAA72858)
Full Name
RECOMBINANT ANTIGEN p18 from Epstein-Barr Virus (EBV)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)
E2F-1, Monoclonal Antibody (Cat# AAA320395)
Full Name
E2F-1 Mouse mAb
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing