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▼Active Proteins
AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 650-700 of 2567 product results
Sequence
Colony Stimulating Factor 1, Macrophage (MCSF), Active Protein (Cat# AAA150971)
Full Name
Active Colony Stimulating Factor 1, Macrophage (MCSF)
Gene Names
Csf1; op; Csfm; MCSF; C87615
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Sequence
Sex Hormone Binding Globulin (SHBG), Active Protein (Cat# AAA150974)
Full Name
Active Sex Hormone Binding Globulin (SHBG)
Gene Names
SHBG; ABP; SBP; TEBG
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
SDS_PAGE
Perforin 1 (PRF1), Active Protein (Cat# AAA152021)
Full Name
Active Perforin 1 (PRF1)
Gene Names
PRF1; P1; PFP; FLH2; PFN1; HPLH2
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>80%
Pricing
SDS-PAGE
Interferon alpha-2, Active Protein (Cat# AAA114580)
Full Name
Recombinant Human Interferon alpha-2
Gene Names
IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
Application Data
IL10, Active Protein (Cat# AAA214314)
Full Name
Recombinant Mouse IL10
Gene Names
Il10; CSIF; Il-10
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
MCP-2 (CCL8), Active Protein (Cat# AAA214330)
Full Name
Recombinant Mouse MCP-2 (CCL8)
Gene Names
Ccl8; HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
GRO gamma (CXCL3), Active Protein (Cat# AAA214268)
Full Name
Recombinant Human GRO gamma (CXCL3)
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
SHH, Active Protein (Cat# AAA214271)
Full Name
Recombinant Human SHH
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
IL36B, Active Protein (Cat# AAA214303)
Full Name
Recombinant Human IL36B
Gene Names
IL36B; FIL1; FIL1H; IL1F8; IL1H2; IL-1F8; IL-1H2; IL1-ETA; FIL1-(ETA); FILI-(ETA)
Purity
>95% as determined by SDS-PAGE
Pricing
Gene Sequence
Interferon Beta (IFNb), Active Protein (Cat# AAA150973)
Full Name
Active Interferon Beta (IFNb)
Gene Names
IFNB1; IFB; IFF; IFNB
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast growth factor 20 (FGF20) is a member of the fibroblast growth factor family. The fibroblast growth factors possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic developme)
Fibroblast Growth Factor 20 (FGF20), Active Protein (Cat# AAA153120)
Full Name
Active Fibroblast Growth Factor 20 (FGF20)
Gene Names
FGF20; RHDA2; FGF-20
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Fibroblast Growth Factor 11 (FGF11) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development,)
Fibroblast Growth Factor 11 (FGF11), Active Protein (Cat# AAA153121)
Full Name
Active Fibroblast Growth Factor 11 (FGF11)
Gene Names
FGF11; FHF3; FHF-3; FGF-11
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Histone Deacetylase 6 (HDAC6) belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains that appear to function independently of each other. This protein possesses histone deacetylase act)
Histone Deacetylase 6 (HDAC6), Active Protein (Cat# AAA153129)
Full Name
Active Histone Deacetylase 6 (HDAC6)
Gene Names
HDAC6; HD6; JM21
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Hydroxysteroid (17beta) dehydrogenase 1 (HSD17b1) is a steroidogenic enzyme that especially catalyzes the conversion of low potent 17keto-steroids to highly potent 17beta-hydroxysteroids. It has a dual function in estrogen activation and androgen inactiva)
17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1), Active Protein (Cat# AAA153130)
Full Name
Active 17-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD17b1)
Gene Names
HSD17B1; HSD17; EDHB17; EDH17B2; SDR28C1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Carboxylesterase 2 (CES2) is a member of serine esterase family which can hydrolyze ester and amide bonds. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic ester)
Carboxylesterase 2 (CES2), Active Protein (Cat# AAA153132)
Full Name
Active Carboxylesterase 2 (CES2)
Gene Names
CES2; iCE; CE-2; PCE-2; CES2A1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares~40% amino acid (aa) identity with three other R-Spondin family memb)
R-Spondin 1 (RSPO1), Active Protein (Cat# AAA153139)
Full Name
Active R-Spondin 1 (RSPO1)
Gene Names
RSPO1; RSPO; CRISTIN3
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Vascular cell adhesion protein 1 (VCAM-1) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracel)
Vascular Cell Adhesion Molecule 1 (VCAM1), Active Protein (Cat# AAA153038)
Full Name
Active Vascular Cell Adhesion Molecule 1 (VCAM1)
Gene Names
VCAM1; CD106; INCAM-100
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 1 beta (IL-1beta) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important medi)
Interleukin 1 Beta (IL1b), Active Protein (Cat# AAA153046)
Full Name
Active Interleukin 1 Beta (IL1b)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular superoxide dismutase [Cu-Zn] is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide r)
Superoxide Dismutase 2, Mitochondrial (SOD2), Active Protein (Cat# AAA153068)
Full Name
Active Superoxide Dismutase 2, Mitochondrial (SOD2)
Gene Names
Sod2; MnSOD; Sod-2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Motility Related Protein (MRP1) is a member of the superfamily of ATP-binding cassette (ABC) transporters. It can enable ATPase-coupled inorganic anion transmembrane transporter activity. Involved in anion transmembrane transport ; renal tubular secretion)
Motility Related Protein (MRP1), Active Protein (Cat# AAA153071)
Full Name
Active Motility Related Protein (MRP1)
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Beta Defensin-1 (DEFB1) displays potent microcidal properties, and also plays a part in other aspects of innate and adaptive immunity. DEFB1 genetic variations have been reported as contributing to hBD1 production impairment, leading to a greater suscepti)
Defensin Beta 1 (DEFb1), Active Protein (Cat# AAA153082)
Full Name
Active Defensin Beta 1 (DEFb1)
Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B)
B-Lymphocyte Activation Antigen B7-1 (LAB7-1), Active Protein (Cat# AAA153084)
Full Name
Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)
Gene Names
Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Lipopolysaccharide Binding Protein (LBP) is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4.Thi)
Lipopolysaccharide Binding Protein (LBP), Active Protein (Cat# AAA153087)
Full Name
Active Lipopolysaccharide Binding Protein (LBP)
Gene Names
Lbp; Ly88; Bpifd2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44 66 kDa, variably N and O glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids ()
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153096)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. EMMPRIN is 269 amino acids (aa) in l)
Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153100)
Full Name
Active Cluster Of Differentiation 147 (CD147)
Gene Names
Bsg; CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A bindi)
Factor Related Apoptosis (FAS), Active Protein (Cat# AAA152951)
Full Name
Active Factor Related Apoptosis (FAS)
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with recombinant chicken IFN-alpha.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA152952)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
IFNA3; IFNA; IFNA1; IFNA2; IFNA6; IFN-alpha; IFN-gamma
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with IFN-alpha.)
Interferon Alpha (IFNa), Active Protein (Cat# AAA152955)
Full Name
Active Interferon Alpha (IFNa)
Gene Names
IFNA1; IFN1@; IFN-ALPHA-1
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell proliferation of TF-1 cells after stimulated with GM-CSF.)
Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF), Active Protein (Cat# AAA152961)
Full Name
Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Gene Names
CSF2; GMCSF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The dose-effect curve of IL-2 on Splenic lymphocytes cells.)
Interleukin 2 (IL2), Active Protein (Cat# AAA152963)
Full Name
Active Interleukin 2 (IL2)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells.Thus, chemotaxis assay used 24-well microchemotaxis syste)
Interleukin 8 (IL8), Active Protein (Cat# AAA152971)
Full Name
Active Interleukin 8 (IL8)
Gene Names
IL8L2; IL8; CXCL8; EMF-1; CXCLi2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of Hepa1-6 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152984)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFA; cTNF
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNFalpha.)
Tumor Necrosis Factor Alpha (TNFa), Active Protein (Cat# AAA152985)
Full Name
Active Tumor Necrosis Factor Alpha (TNFa)
Gene Names
TNF; TNFA; TNF-a; TNFSF2
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with TNF-beta)
Tumor Necrosis Factor Beta (TNFb), Active Protein (Cat# AAA152996)
Full Name
Active Tumor Necrosis Factor Beta (TNFb)
Gene Names
Lta; Tnfb
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. Cell apoptosis of A549 cells after stimulated with recombinant horse IFNb.)
Interferon Beta (IFNb), Active Protein (Cat# AAA153004)
Full Name
Active Interferon Beta (IFNb)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.)
Galectin 2 (GAL2), Active Protein (Cat# AAA153012)
Full Name
Active Galectin 2 (GAL2)
Gene Names
Lgals2; AI324147; 2200008F12Rik
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL4 in V- bottom shaped 96-well microtiter plate.)
Galectin 4 (GAL4), Active Protein (Cat# AAA153015)
Full Name
Active Galectin 4 (GAL4)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL7 in V- bottom shaped 96-well microtiter plate.)
Galectin 7 (GAL7), Active Protein (Cat# AAA153020)
Full Name
Active Galectin 7 (GAL7)
Gene Names
LGALS7B; PI7; GAL7; Gal-7; HKL-14; LGALS7
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL8 in V- bottom shaped 96-well microtiter plate.)
Galectin 8 (GAL8), Active Protein (Cat# AAA153022)
Full Name
Active Galectin 8 (GAL8)
Gene Names
LGALS8; Gal-8; PCTA1; PCTA-1; Po66-CBP
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL12 in V- bottom shaped 96-well microtiter plate.)
Galectin 12 (GAL12), Active Protein (Cat# AAA153028)
Full Name
Active Galectin 12 (GAL12)
Gene Names
Lgals12; Grip1; AW987437
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Figure 2. The hemagglutination assay of GAL1 in V- bottom shaped 96-well microtiter plate.)
Galectin 1 (GAL1), Active Protein (Cat# AAA153031)
Full Name
Active Galectin 1 (GAL1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Sequence
Galectin 8 (GAL8), Active Protein (Cat# AAA152175)
Full Name
Active Galectin 8 (GAL8)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Galectin-12 is a member of a family of mammalian lectins known as galectins. The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Galectin-12 is predominantly expressed in adipose tissue and detected also in macrophages and other leukocytes. It plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Po GAL12 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL12 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL12, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL12 is 0.195 ug/mL.)
Galectin 12 (GAL12), Active Protein (Cat# AAA152176)
Full Name
Active Galectin 12 (GAL12)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Troponin T Type 2, Cardiac (TNNT2) is one of three troponin isoforms found in the tropomyosin-troponin complex. This complex is responsible for the calcium sensitivity of the contractile apparatus in the muscle. Cardiac Troponin T is used as a biological marker for cardiomyocytes and its level in serum is frequently used as an indicator of myocardial cell injury. Besides, Troponin I Type 3, Cardiac (TNNI3) has been identified as an interactor of TNNT2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TNNT2 and recombinant human TNNTI3. Briefly, TNNT2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to TNNI3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TNNT2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450/630nm immediately. The binding activity of TNNT2 and TNNI3 was shown in Figure 1, and this effect was in a dose dependent manner.)
Troponin T Type 2, Cardiac (TNNT2), Active Protein (Cat# AAA152235)
Full Name
Active Troponin T Type 2, Cardiac (TNNT2)
Gene Names
TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
SDS-PAGE
(SDS-PAGE)
Interferon Epsilon (IFNe), Active Protein (Cat# AAA152349)
Full Name
Active Interferon Epsilon (IFNe)
Gene Names
IFNE; IFN-E; IFNE1; IFNT1; INFE1; PRO655
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(Brain-derived neurotrophic factor, also known as BDNF, is a member of the neurotrophin family of growth factors, which are related to the canonical Nerve Growth Factor. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. Besides, Sortilin 1 (SORT1) has been identified as an interactor of BDNF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human BDNF and recombinant human SORT1. Briefly, SORT1 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to BDNF-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SORT1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450/630nm immediately. The binding activity of of BDNF and SORT1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Brain Derived Neurotrophic Factor (BDNF), Active Protein (Cat# AAA152273)
Full Name
Active Brain Derived Neurotrophic Factor (BDNF)
Gene Names
BDNF; ANON2; BULN2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Glypican 1 (GPC1) is one of the six glycosylphosphatidylinositol-anchored, cell surface heparan sulfate proteoglycans that acts as a growth factor signaling. Cells known to express GPC-1 include neurons, smooth and skeletal muscle cells, keratinocytes, osteoblasts, Schwann cells, immature dendritic cells, and tumor, plus tumor-associated vascular endothelial cells. The function of GPC-1 is complex and varied, it can modulate various signaling pathways including Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), fibroblast growth factors (FGF), vascular endothelial growth factor-A (VEGF-A), transforming growth factor-beta (TGF-beta), Wnt, Hedgehog (Hh), and bone morphogenic protein (BMP) through specific interactions with pathway ligands and receptors. Besides, Syndecan 4 (SDC4) has been also identified as an interactor of GPC1), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse GPC1 and recombinant chicken SDC4. Briefly, GPC1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SDC4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse GPC1 and recombinant chicken SDC4 was shown in Figure 1, the EC50 for this effect is 0.04 ug/mL.)
Glypican 1 (GPC1), Active Protein (Cat# AAA161825)
Full Name
Active Glypican 1 (GPC1)
Gene Names
Gpc1; AI462976
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Interferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, is a transcription factor that plays central roles in the regulation of myeloid cell fate. In both mice and humans, IRF8 is required for the differentiation of most monocyte and dendritic cell subsets, but suppresses neutrophil production. IRF8 can both act as a transcriptional activator or repressor, and it acts as a transcriptional repressor of osteoclast differentiation factors such as NFATC1 and EEIG1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse IRF8 and recombinant mouse NFATC1. Briefly, IRF8 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to NFATC1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IRF8 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse IRF8 and recombinant mouse NFATC1 was shown in Figure 1, the EC50 for this effect is 0.05 ug/mL.)
Interferon Regulatory Factor 8 (IRF8), Active Protein (Cat# AAA161858)
Full Name
Active Interferon Regulatory Factor 8 (IRF8)
Gene Names
Irf8; Myls; ICSBP; IRF-8; Icsbp1; AI893568
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(EphrinB2 (EFNB2), a 40 kDa member of the EphrinB family, is a pivotal bidirectional signaling molecule ubiquitously expressed in mammals and is crucial in angiogenesis during development and disease progression. Besides, EFNB2 is expressed at abnormally high levels in some neoplasms, such as squamous cell carcinoma of the head and neck and colorectal cancer. Its overexpression is associated with the malignant progression of tumors. Reelin (RELN) has been identified as an interactor of EFNB2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNB2 and recombinant human RELN. Briefly, EFNB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to RELN-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EFNB2 and recombinant human RELN was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
Ephrin B2 (EFNB2), Active Protein (Cat# AAA161920)
Full Name
Active Ephrin B2 (EFNB2)
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, Balb/c 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 8, 000 cells/well and allowed to attach overnight, then the medium was replaced with 0.4% FBS standard DMEM prior to the addition of various concentrations of recombinant human EGF. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450 nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Cell proliferation of Balb/c 3T3 cells after incubation with rhEGF for 48h observed by inverted microscope was shown in Figure 1. The dose-effect curve of rhEGF was shown in Figure2. It was obvious that rhEGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 4.2 ng/ml.)
Epidermal Growth Factor (EGF), Active Protein (Cat# AAA161748)
Full Name
Active Epidermal Growth Factor (EGF)
Gene Names
EGF; URG; HOMG4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.