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WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TOMM22 monoclonal antibody. Western Blot analysis of TOMM22 expression in PC-12.)
WB (Western Blot) (Western blot analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (41.73kD).)
WB (Western Blot) (Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml])
WB (Western Blot) (Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.36kD).)
Application Data (Detection limit for recombinant GST tagged MEIS2 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEIS2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody. Lane 1: MEIS2 transfected lysate (51.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in K-562.)
WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)
WB (Western Blot) (CA1 monoclonal antibody, Western Blot analysis of CA1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged CA1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CA1 transfected lysate using 124177 and Protein A Magnetic Bead and immunoblotted with CA1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of CA1 expression in transfected 293T cell line using 124177. Lane 1: CA1 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of CA1 expression in human spleen using 124177)
WB (Western Blot) (Western Blot detection against Immunogen (54.45kD), using 124177.)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Securin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Securin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Securin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Securin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Securin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Securin on SiHa cell lysate using anti-Securin antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.)
IHC (Immunohistchemistry) (Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [11C12]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.Red: CD80 Antibody [11C12]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [11C12]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
WB (Western Blot) (Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])
Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (TAF11 monoclonal antibody (M06), clone 3G6 Western Blot analysis of TAF11 expression in Hela S3 NE (Cat # L013V3).)
WB (Western Blot) (Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, Jurkat, HDLM-2, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation) (Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2) used for IP at 20 ul/mg lysate. LAG3 was also immunoprecipitated by a previous lot of this antibody (AAA23802 lot 1) and a second antibody against a different epitope of LAG3 . For blotting immunoprecipitated LAG3, AAA23802 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human LAG3 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human LAG3 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human LAG3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23802) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody.Lane 1: PIP5K3 transfected lysate (50.2kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in PC-12.)
WB (Western Blot) (MGC21874 monoclonal antibody, Western Blot analysis of MGC21874 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged MGC21874 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MGC21874 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in NIH/3T3.)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
WB (Western Blot) (Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged DUSP6 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10ug/ml.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody. Lane 1: DUSP6 transfected lysate (42kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)
WB (Western Blot) (Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
Simple Western (Detection of mouse PBRM1 by Simple Western. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)
IP (Immunoprecipitation) (Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used for IP at 5 ul/mg lysate. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23796-3). For blotting immunoprecipitated PBRM1, AAA23796 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry) (Detection of human PBRM1 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human PBRM1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (AAA23796 lot 4) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
IF (Immunofluorescence) (Fig.6. Immunofluorescence analysis of rat brain tissue. 1, GFAP Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
IF (Immunofluorescence) (Fig.5. Immunofluorescence analysis of mouse brain tissue. 1, GFAP Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
IHC (Immunohistochemistry) (Fig.4. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, GFAP Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Fig.3. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue. 1, GFAP Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Fig.2. Immunohistochemical analysis of paraffin-embedded human liver tissue. 1, GFAP Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Fig.1. Western blot analysis of rat brain tissue, diluted at 1:5000.)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:RPE)
Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)
Application Data (Immunofluorescence image of human tonsil cryosection stained with Sheep anti Human LOX-1 antibody in red and with Mouse anti Human CD21 antibody in red. Nuclei are stained blue using DAPI. Merged image on right)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)
Application Data (Immunoperoxidase staining of human spleen cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)
WB (Western Blot) (Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody. Lane 1: CAMK4 transfected lysate (52.14kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMK4 monoclonal antibody Western Blot analysis of CAMK4 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (77.66kD).)
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (TAF11 monoclonal antibody (M04), clone 2G9 Western Blot analysis of TAF11 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining ATF5 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ATF5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATF5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ATF5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ATF5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ATF5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ATF5 on different lysates using anti-ATF5 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: PC-12)
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)
Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. The merged image is in C with nuclei counterstained blue using DAPI. High power)
Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. The merged image is in C with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Medium power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Low power)
Application Data (Staining of KM-H2 cells with Mouse anti Human CD83)
Application Data (Staining of KM-H2 cell line with Mouse anti Human CD83:FITC)
WB (Western Blot) (Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2) used for IP at 12 ul per reaction. LAG3 was also immunoprecipitated by a previous lot of this antibody (lot AAA23801-1). For blotting immunoprecipitated LAG3, AAA23801 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human LAG3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human LAG3 in FFPE HDLM-2 by immunocytochemistry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human Lag3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-Lag3 recombinant monoclonal antibody (AAA23801) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~ 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5ug/ml])
WB (Western Blot) (PHB monoclonal antibody. Western Blot analysis of PHB expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (55.66kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
WB (Western Blot) (CAMKK1 monoclonal antibody, Western Blot analysis of CAMKK1 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged CAMKK1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody. Lane 1: CAMKK1 transfected lysate (57.31kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (83.31kD).)
Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)
WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)
WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in NIH/3T3 using AAA24054.)
WB (Western Blot) (Western Blot analysis of PLA2G15 expression in transfected 293T cell line using AAA24054. Lane 1: PLA2G15 transfected lysate (46.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in Raw 264.7 using AAA24054.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in PC-12 using AAA24054.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in HeLa using AAA24054.)
WB (Western Blot) (Western Blot detection against Immunogen using AAA24054 (37kD) .)
WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])
WB (Western Blot) (Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (87.56kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GSTZ1 on HepG2 cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of GSTZ1 expression in transfected 293T cell line by GSTZ1 monoclonal antibody. Lane 1: GSTZ1 transfected lysate (24.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in NIH/3T3.)
WB (Western Blot) (GSTZ1 monoclonal antibody, Western Blot analysis of GSTZ1 expression in HepG2.)
WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
WB (Western Blot) (C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged C22orf18 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CENPM transfected lysate using CENPM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPM rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody. Lane 1: CENPM transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (45.91kD).)
CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2) (HRP)
Gene Names
CENPM; PANE1; CENP-M; C22orf18
Reactivity
Human
Applications
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Data (Detection limit for recombinant GST tagged FOXA1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FOXA1 monoclonal antibody (M02), clone 1B1 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
WB (Western Blot) (Detection of mouse SMC1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of human SMC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Jurkat, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795 lot 3) used for IP at 20 ul/mg lysate. SMC1 was also immunoprecipitated by a previous lot of this antibody (AAA23795 lot 2) and a second antibody against a different epitope of SMC1 . For blotting immunoprecipitated SMC1, AAA23795 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistchemistry) (Detection of human SMC1 in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry) (Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human SMC1 by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (AAA23795 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse SMC1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (AAA23795) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human SMC1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (AAA23795) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])
WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)
WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)
WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody Lane 1: VRK2 transfected lysate (58.1kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged VRK2 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (VRK2 monoclonal antibody, Western Blot analysis of VRK2 expression in K-562.)
WB (Western Blot) (VRK2 monoclonal antibody. Western Blot analysis of VRK2 expression in U-2 OS.)
Application Data (Detection limit for recombinant GST tagged PGGT1B is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml])
WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in PC-12.)
WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.29kD).)
PGGT1B (Geranylgeranyl Transferase Type-1 Subunit beta, Geranylgeranyl Transferase Type I Subunit beta, GGTase-I-beta, Type I Protein Geranyl-Geranyltransferase Subunit beta) (AP)
Reactivity
Human, Rat
Applications
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
WB (Western Blot) (SMURF1 monoclonal antibody, Western Blot analysis of SMURF1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
Application Data (Detection limit for recombinant GST tagged SMURF1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SMURF1 transfected lysate using SMURF1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SMURF1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2ug/ml].)
WB (Western Blot) (Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody. Lane 1: SMURF1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])
WB (Western Blot) (ROCK2 monoclonal antibody (M02), clone 1E12 Western Blot analysis of ROCK2 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistchemistry) (Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.)
IHC (Immunohistochemistry) (Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [7A2]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.Red: CD80 Antibody [7A2]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [7A2]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488)
Application Data (Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)
WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western blot analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ABCF2 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ABCF2 transfected lysate using anti-ABCF2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ABCF2 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABCF2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ABCF2 expression in transfected 293T cell line by ABCF2 monoclonal antibody: Lane 1: ABCF2 transfected lysate (68.53kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABCF2 monoclonal antibody Western Blot analysis of ABCF2 expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
Application Data (Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody. Lane 1: SNRPA transfected lysate (31.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)
Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PTBP1 transfected lysate using PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PTBP1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody, Lane 1: PTBP1 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PTBP1 monoclonal antibody, Western Blot analysis of PTBP1 expression in A-431.)
PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (HRP)
WB (Western Blot) (Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NMI is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody. Lane 1: NMI transfected lysate (35.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NMI monoclonal antibody Western Blot analysis of NMI expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.)
WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BST2 MaxPab rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD137 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining CD137 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD137 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD137 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD137 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD137 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue using anti-CD137 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD137 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD137 on HepG2 cell lysate using anti-CD137 antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Radixin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Radixin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Radixin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Radixin on different lysates using anti-Radixin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2)
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Purity
ProA affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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