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SDS-PAGE (SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8(ABT535) antibody. The HRP-conjugated Goat anti-M)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
WB (Western Blot) (ACATE2 monoclonal antibody Western Blot analysis of ACATE2 expression in MCF-7.)
Application Data (Detection limit for recombinant GST tagged ACOT9 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody. Lane 1: ACOT9 transfected lysate (24kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (49.43kD).)
WB (Western Blot) (Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody. Lane 1: NFIC transfected lysate (47.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (72.82kD).)
ICC (Immunocytochemistry) (ICC staining HDAC3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HDAC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-HDAC3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HDAC3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HDAC3 on NIH/3T3 cell lysates using anti-HDAC3 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of THP-1 cells with Iba1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining Iba1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Iba1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Iba1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Iba1 antibody. Counter stained with hematoxylin.)
SDS-PAGE (Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Claudin 7 (ABT443) antibody. The HRP-conjugated Goat anti-M)
Application Data (Detection limit for recombinant GST tagged NR3C1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NR3C1 expression in transfected 293T cell line by NR3C1 monoclonal antibody. Lane 1: NR3C1 transfected lysate (85.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NR3C1 monoclonal antibody, Western Blot analysis of NR3C1 expression in HeLa NE.)
WB (Western Blot) (NR3C1 monoclonal antibody. Western Blot analysis of NR3C1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Detection limit for recombinant GST tagged HMGB1 is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (HMGB1 monoclonal antibody (M08), clone 2F6 Western Blot analysis of HMGB1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody Lane 1: VRK2 transfected lysate (58.1kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged VRK2 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (VRK2 monoclonal antibody, Western Blot analysis of VRK2 expression in K-562.)
WB (Western Blot) (VRK2 monoclonal antibody. Western Blot analysis of VRK2 expression in U-2 OS.)
WB (Western Blot) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)
WB (Western Blot) (NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.)
Application Data (Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody. Lane 1: NEK11 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (79.13kD).)
Application Data (Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.)
Application Data (Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.)
WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in human kidney.)
WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in MCF-7.)
Application Data (PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml])
ICC (Immunocytochemistry) (ICC staining hnRNP K in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining hnRNP K in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining hnRNP K in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-hnRNP K antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of hnRNP K on different lysates using anti-hnRNP K antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: NIH/3T3)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.Lane 1: FTL transfected lysate (20 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3. GAPDH (36.1 kDa) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562 (Cat # L009V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot) (Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000, 1:512000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: MCF-7 whole cell lysate at 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: Mouse Brain tissue, Mouse Skeldtal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1min)
SDS-PAGE (SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8(ABT534) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged C22orf18 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CENPM transfected lysate using CENPM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPM rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody. Lane 1: CENPM transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (45.91kD).)
CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2) (Biotin)
Gene Names
CENPM; PANE1; CENP-M; C22orf18
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
WB (Western Blot) (NEK10 monoclonal antibody, Western Blot analysis of NEK10 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged NEK10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody. Lane 1: NEK10 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in NIH/3T3.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in Raw 264.7.)
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in PC-12.)
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1 Western Blot analysis of CDC2 expression in Hela S3 NE.)
WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M16), clone X1.Lane 1: FTL transfected lysate (20 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M16), clone X1. GAPDH (36.1 kDa) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FTL monoclonal antibody (M16), clone X1 Western Blot analysis of FTL expression in K-562 (Cat # L009V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (SMURF1 monoclonal antibody, Western Blot analysis of SMURF1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
Application Data (Detection limit for recombinant GST tagged SMURF1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SMURF1 transfected lysate using SMURF1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SMURF1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2ug/ml].)
WB (Western Blot) (Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody. Lane 1: SMURF1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in PC-12.)
WB (Western Blot) (NEK9 monoclonal antibody Western Blot analysis of NEK9 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged NEK9 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody. Lane 1: NEK9 transfected lysate (107.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Vimentin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second antibody)
ICC (Immunocytochemistry) (ICC staining Vimentin in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Vimentin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Vimentin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Vimentin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Vimentin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Vimentin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Vimentin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Vimentin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Vimentin on different lysates using anti-Vimentin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A549)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)
WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)
WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
Application Data (Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.)
WB (Western Blot) (GLUD2 monoclonal antibody (M01), clone 3C2. Western Blot analysis of GLUD2 expression in human pancreas.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate (61 KDa).Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged GBA is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GBA on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody. Lane 1: GBA transfected lysate (60kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GBA monoclonal antibody, Western Blot analysis of GBA expression in MCF-7.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 Huh7 cells were stained with PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with PIK3R1 rabbit purified polyclonal 1:1200 and SHC1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged SHC1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SHC1 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].)
WB (Western Blot) (SHC1 monoclonal antibody, Western Blot analysis of SHC1 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
SHC (SHC-transforming Protein 1, SH2 Domain Protein C1, SHC1, SHC-transforming Protein 3, SHC-transforming Protein A, SHCA, Src Homology 2 Domain-containing-transforming Protein C1, FLJ26504) (FITC)
Gene Names
SHC1; SHC; SHCA
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
WB (Western Blot) (PKNOX2 monoclonal antibody. Western Blot analysis of PKNOX2 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody. Lane 1: PKNOX2 transfected lysate (51.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PKNOX2 monoclonal antibody, Western Blot analysis of PKNOX2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M18), clone X3.Lane 1: FTL transfected lysate(20 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M18), clone X3. GAPDH (36.1 kDa) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (FTL monoclonal antibody (M18), clone X3 Western Blot analysis of FTL expression in K-562.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of LMNB1 transfected lysate using anti-LMNB1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LMNB1 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LMNB1 expression in transfected 293T cell line by LMNB1 monoclonal antibody (M01), clone 4B10.Lane 1: LMNB1 transfected lysate (66.4 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Raw 264.7.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in NIH/3T3.)
WB (Western Blot) (LMNB1 monoclonal antibody (M01), clone 4B10. Western Blot analysis of LMNB1 expression in Jurkat (Cat # L017V1).)
FCM (Flow Cytometry) (Overlay histogram showing HepG2 cells stained with AAA28062 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with AAA28062 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry) (Overlay histogram showing A549 cells stained with AAA28062 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells with AAA28062 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with AAA28062 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with AAA28062 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IHC (Immunohistchemistry) (IHC image of AAA28062 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image of AAA28062 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image of AAA28062 diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (Western BlotPositive WB detected in: A375 whole cell lysate, Rabbit spleen tissueAll lanes CD63 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 30-120 KD KDaObserved band size: 30-120 KD KDaExposure time?1min)
WB (Western Blot) (Western BlotPositive WB detected in: Raji whole cell lysateAll lanes CD63 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 30-120 KD KDaObserved band size: 30-120 KD KDaExposure time?1min)
WB (Western Blot) (Western BlotPositive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysateAll lanes CD63 antibody at 1:1000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 30-120 KD KDaObserved band size: 30-120 KD KDaExposure time?1min)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD8 a(ABT455) antibody. The HRP-conjugated Goat anti-M)
Application Data (Detection limit for recombinant GST tagged GCH1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GCH1 transfected lysate using 127227and Protein A Magnetic Bead and immunoblotted with GCH1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human lymph node using 127227 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of GCH1 expression in transfected 293T cell line using 127227. Lane 1: GCH1 transfected lysate (27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of GCH1 using 127227 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Detection limit for recombinant GST tagged PLK1 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody. Lane 1: PLK1 transfected lysate (68.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using PLK1 monoclonal antibody.)
WB (Western Blot) (PLK1 monoclonal antibody Western Blot analysis of PLK1 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (91.85kD).)
Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS7 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in NIH/3T3.)
WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in Raw 264.7.)
WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in PC-12.)
WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in HeLa.)
WB (Western Blot) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)
WB (Western Blot) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate (52.9 KDa).Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using AMPKa1/AMPKa2 Mouse mAb (AAA28599) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Moesin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Moesin in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Moesin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Moesin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Moesin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Moesin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Moesin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Moesin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Moesin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Moesin on different lysates using anti-Moesin antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: SH-SY-5Y)
IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
FCM (Flow Cytometry) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
FCM (Flow Cytometry) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488 (AAA11960A488))
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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