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FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: Hela)
WB (Western Blot) (F11R monoclonal antibody, Western Blot analysis of F11R expression in HepG2.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with PRKCZ rabbit purified polyclonal 1:1200 and F11R mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged F11R is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody. Lane 1: F11R transfected lysate (32.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (59kD).)
Application Data (Detection limit for recombinant GST tagged FGR is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml.)
WB (Western Blot) (Western Blot analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody. Lane 1: FGR transfected lysate (59.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in Raw 264.7.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in PC-12.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil stained with C2399-07G2, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)
IHC (Immunohistochemistry) (immunohistochemical analysis of an acetone fixed, frozen human colon section using C2399-07G2.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with MEKK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining MEKK2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEKK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MEKK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MEKK2 on different lysates using anti-MEKK2 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Rat brain Lane 3: SW480)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Hela)
SDS-PAGE (Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-N-Cadherin(ABT-CDH2) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (Western blot analysis of N-CadherinAntibody at 1:1000 dilution.)
IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)
WB (Western Blot) (Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (AKAP10 monoclonal antibody Western Blot analysis of AKAP10 expression in Raw 264.7.)
WB (Western Blot) (RNAi Knockdown: Western blot analysis of AKAP10-over-expressing 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with Cat. # 123131. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged AKAP10 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase staining of AKAP10 on formalin-fixed paraffin-embedded human testis using Cat. # 123131 at 0.5ug/ml.)
WB (Western Blot) (Western Blot analysis of AKAP10 expression in transfected 293T cell line using Cat. # 123131: Lane 1: AKAP10 transfected lysate (73.8kD); Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
AKAP10 (A-kinase Anchor Protein 10, Mitochondrial, AKAP-10, Dual Specificity A Kinase-anchoring Protein 2, D-AKAP-2, Protein Kinase A-anchoring Protein 10, PRKA10) (FITC)
Gene Names
AKAP10; PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
Reactivity
Human, Mouse
Applications
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M02), clone 4A5 Western Blot analysis of YY1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in PC-12 (Cat # L012V1).)
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).)
Application Data (Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1 Western Blot analysis of CDC2 expression in Hela S3 NE (Cat # L013V3).)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat brain tissue using Alpha-synuclein Mouse mAb (AAA28601, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse brain tissue using Alpha-synuclein Mouse mAb (AAA28601, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human brain tissue using Alpha-synuclein Mouse mAb (AAA28601, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of HEL cells using Alpha-synuclein Mouse mAb (AAA28601, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Alpha-synuclein Mouse mAb (AAA28601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Alpha-synuclein Mouse mAb (AAA28601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Alpha-synuclein Mouse mAb (AAA28601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Alpha-synuclein Mouse mAb (AAA28601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Alpha-synuclein Mouse mAb (AAA28601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Alpha-synuclein Mouse mAb (AAA28601) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Alpha-synuclein Mouse mAb (AAA28601) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colon)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MSH6 and anti-GAPDH antibody. The HRP-conjugated)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MSH6 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD) .)
WB (Western Blot) (RBM9 monoclonal antibody Western Blot analysis of RBM9 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged RBM9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RBM9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibodyLane 1: RBM9 transfected lysate (40.4kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa NE.)
Application Data (Detection limit for recombinant GST tagged CHEK2 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
Application Data (Detection limit for recombinant GST tagged TP53 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (TP53 monoclonal antibody (M01), clone 2C3 Western Blot analysis of TP53 expression in A-431.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TP53 on A-431 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TP53 on A-431 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (Confocal imaging of NIH/3T3 cells using Calreticulin Mouse mAb (AAA28600, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Calreticulin Mouse mAb (AAA28600) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Calreticulin Mouse mAb (AAA28600) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Calreticulin Mouse mAb (AAA28600) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Calreticulin Mouse mAb (AAA28600) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from C6 cells using Calreticulin Mouse mAb (AAA28600) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
WB (Western Blot) (Western blot analysis of various lysates using Calreticulin Mouse mAb (AAA28600) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MSH2 (ABT-MSH2) antibody. The HRP-conjugated)
SDS-PAGE (Jurkat whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-ZAP-70(ABT246)antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)
Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJC10 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)
WB (Western Blot) (Western blot analysis of FOXA1 over-expressed 293 cell line, cotransfected with FOXA1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA1 expression in transfected 293T cell line by FOXA1 monoclonal antibody Lane 1: FOXA1 transfected lysate (49.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA1 monoclonal antibody Western Blot analysis of FOXA1 expression in HepG2)
WB (Western Blot) (Western Blot detection against Immunogen (37.4kD).)
WB (Western Blot) (Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IP (Immunoprecipitation) (Immunoprecipitation of ALPPL2 transfected lysate using ALPPL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALPPL2 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody Lane 1: ALPPL2 transfected lysate (57.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ALPPL2 monoclonal antibody Western Blot analysis of ALPPL2 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged SAV1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SAV1 transfected lysate using SAV1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SAV1 on HeLa cell. [antibody concentration 60ug/ml].)
WB (Western Blot) (SAV1 monoclonal antibody. Western Blot analysis of SAV1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (35.35kD).)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Published customer image: Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p)
Application Data (Staining of rat spleen cells with Mouse anti Rat CD25: FITC)
Application Data (Published clone specific image: The percentage of Ly49s3+ NK cells increases in the spleen and decreases in the bone marrow following L. monocytogenes infection. A) Mononuclear leukocytes were harvested from spleen, blood, and bone marrow from uninfected and infected animals 24 and 48 hrs p.i. Cells were stained with antibodies to NKR-P1A, CD3, and Ly49s3. NK cells were gated as NKR-P1A+CD3- cells, and the percentage of Ly49s3+ NK cells was analysed by flow cytometry. The data represent the mean +/- SEM from three independent experiments, with 2 animals at each time point. Data were analyzed with the one-way ANOVA with Tukey's multiple comparisons test. B) The activation status of NK cells from uninfected (filled histogram) or infected animals 24 (grey line) and 48 hrs p.i. (black line) was tested by flow cytometry. An antibody towards the activation marker CD25 was employed.From: Shegarfi H, Naper C, Rolstad B, Inngjerdingen M (2010) Listeria monocytogenes Infection Affects a Subset of Ly49-Expressing NK Cells in the Rat. PLoS ONE 5(12): e15579.)
Application Data (Staining of stimulated rat spleen cells with Mouse anti Rat CD25:FITC)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Staining of Con A activated rat spleen cells with Mouse anti Rat CD25: Biotin)
WB (Western Blot) (FLJ20097 monoclonal antibody, Western Blot analysis of FLJ20097 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ20097 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FLJ20097 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in NIH/3T3.)
WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in human pancreas.)
WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TOMM22 monoclonal antibody. Western Blot analysis of TOMM22 expression in PC-12.)
WB (Western Blot) (Western blot analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (41.73kD).)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in Raw 264.7 (Cat # L024V1).)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in PC-12 (Cat # L012V1).)
Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10 Western Blot analysis of FGR expression in HeLa (Cat # L013V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western Blot detection against Immunogen (70kD).)
WB (Western Blot) (Western blot analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LEF1 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody. Lane 1: LEF1 transfected lysate (44.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (LEF1 monoclonal antibody, Western Blot analysis of LEF1 expression in MES-SA/Dx5.)
WB (Western Blot) (LEF1 monoclonal antibody. Western Blot analysis of LEF1 expression in HL-60.)
WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)
WB (Western Blot) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
WB (Western Blot) (Western Blot detection against Immunogen (84.74kD).)
WB (Western Blot) (PHGDH monoclonal antibody. Western Blot analysis of PHGDH expression in human liver.)
IP (Immunoprecipitation) (Immunoprecipitation of PHGDH transfected lysate using PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1-10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1-10ug/ml])
WB (Western Blot) (Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody. Lane 1: PHGDH transfected lysate (56.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged HMGB1 is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (HMGB1 monoclonal antibody (M08), clone 2F6 Western Blot analysis of HMGB1 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to G3BP on HeLa cell (antibody concentration 10ug/ml).)
WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431.)
WB (Western Blot) (Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged GCH1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GCH1 transfected lysate using 127227and Protein A Magnetic Bead and immunoblotted with GCH1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human lymph node using 127227 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of GCH1 expression in transfected 293T cell line using 127227. Lane 1: GCH1 transfected lysate (27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of GCH1 using 127227 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Detection limit for recombinant GST tagged UBE2C is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody. Lane 1: UBE2C transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UBE2C monoclonal antibody, Western Blot analysis of UBE2C expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
Application Data (Detection limit for recombinant GST tagged PLK1 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody. Lane 1: PLK1 transfected lysate (68.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using PLK1 monoclonal antibody.)
WB (Western Blot) (PLK1 monoclonal antibody Western Blot analysis of PLK1 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (91.85kD).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NLRP3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Human small intestine tissue was stained with Anti-Villin (ABT097) Antibody)
IHC (Immunohistchemistry) (Human kidney tissue was stained with Anti-Villin (ABT097) Antibody)
IHC (Immunohistochemistry) (Human colon carcinoma tissue was stained with Anti-Villin (ABT097) Antibody)
IHC (Immunohistochemistry) (Human colon tissue was stained with Anti-Villin (ABT097) Antibody)
IHC (Immmunohistochemistry) (Human appendix tissue was stained with Anti-Villin (ABT097) Antibody)
SDS-PAGE (Whole cell lysates of HepG2 were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Villin antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
SDS-PAGE (Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Villin antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
WB (Western Blot) (ASB9 monoclonal antibody, Western Blot analysis of ASB9 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged ASB9 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ASB9 transfected lysate using ASB9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ASB9 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody.Lane 1: ASB9 transfected lysate (31.9kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (53.83kD).)
ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954)
Reactivity
Human
Applications
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Purity
Affinity Purified Purified by Protein A affinity chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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