Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Detection limit for recombinant GST tagged SMAD2 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (SMAD2 monoclonal antibody (M05), clone 3G9 Western Blot analysis of SMAD2 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD2 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SMAD2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 5 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with HP1 gamma antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.)
Application Data (Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ADRM1 transfected lysate using ADRM1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ADRM1 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody Lane 1: ADRM1 transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ADRM1 monoclonal antibody Western Blot analysis of ADRM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (69.01kD).)
WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11. Western Blot analysis of FGR expression in Raw 264.7 (Cat # L024V1).)
WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11. Western Blot analysis of FGR expression in PC-12 (Cat # L012V1).)
Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11 Western Blot analysis of FGR expression in HeLa (Cat # L013V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with SATB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining SATB2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SATB2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SATB2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SATB2 on THP-1 cell using anti-SATB2 antibody at 1/500 dilution.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (9C7) Mouse mAb.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells using Bip/GRP78 (9C7) Mouse mAb(dilution 1:100).)
WB (Western Blot) (Western blot detection of Bip/GRP78 in Jurkat,MCF7,Hela cell lysates using Bip/GRP78 (9C7) Mouse mAb(1:2000 diluted). Predicted band size:78KDa. Observed band size:78KDa.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Colon Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)
WB (Western Blot) (Western blot analysis of 1)Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2,000.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FGA and F2. HeLa cells were stained with FGA rabbit purified polyclonal 1:1200 and F2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged F2 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of F2 transfected lysate using F2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F2 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody. Lane 1: F2 transfected lysate (70kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Detection limit for recombinant GST tagged RPS5 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS5 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody. Lane 1: RPS5 transfected lysate (22.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in NIH/3T3.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in Raw 264.7.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in PC-12.)
WB (Western Blot) (RPS5 monoclonal antibody, Western Blot analysis of RPS5 expression in HeLa.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with DKK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DKK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DKK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DKK1 on MCF-7 cells lysates using anti-DKK1 antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPL19 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to RPL19 on formalin-fixed paraffin-embedded human small Intestine tissue. [antibody concentration 1~10ug/ml])
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in NIH/3T3.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in Jurkat.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in Raw 264.7.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in PC-12.)
WB (Western Blot) (RPL19 monoclonal antibody. Western Blot analysis of RPL19 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged PAK1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody. Lane 1: PAK1 transfected lysate (Predicted MW: 49.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PAK1 monoclonal antibody Western Blot analysis of PAK1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2(ABT077) antibody. The HRP-conjugated Goat anti-M)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with STAT5a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining STAT5a in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining STAT5a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT5a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT5a antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of STAT5a on different lysates using anti-STAT5a antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: K562)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with RHOA rabbit purified polyclonal 1:1200 and DAAM1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged DAAM1 is ~1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody.Lane 1: DAAM1 transfected lysate (122.3kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (CPSF6 monoclonal antibody Western Blot analysis of CPSF6 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged CPSF6 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CPSF6 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of CPSF6 expression in transfected 293T cell line by CPSF6 monoclonal antibody. Lane 1: CPSF6 transfected lysate (59.21kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CPSF6 monoclonal antibody Western Blot analysis of CPSF6 expression in Raw 264.7.)
WB (Western Blot) (CPSF6 monoclonal antibody. Western Blot analysis of CPSF6 expression in HL-60.)
WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ALB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ALB transfected lysate using ALB monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALB rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (ALB monoclonal antibody Western Blot analysis of ALB expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (90.75kD).)
Application Data (Detection limit for recombinant GST tagged TP53 is approximately 3ng/ml as a capture antibody.)
WB (Western Blot) (TP53 monoclonal antibody, Western Blot analysis of TP53 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 6ug/ml])
Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and BAX. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and BAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
WB (Western Blot) (Western Blot analysis of TP53 expression in transfected 293T cell line by TP53 monoclonal antibody. Lane 1: TP53 transfected lysate (43.34kD) Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in PC-12)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in Raw 264.7)
Application Data (Detection limit for recombinant GST tagged PGRMC2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGRMC2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml])
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in NIH/3T3)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml])
WB (Western Blot) (SP1 monoclonal antibody (M03), clone 4C8 Western Blot analysis of SP1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
WB (Western Blot) (AKT1 monoclonal antibody Western Blot analysis of AKT1 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody. Lane 1: AKT1 transfected lysate (55.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AKT1 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AKT1 transfected lysate using AKT1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AKT1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (78.54kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM20 on HeLa cell. [antibody concentration 10ug/ml])
Application Data (Detection limit for recombinant GST tagged TOMM20 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human small Intestine using 1346004 (3ug/ml).)
WB (Western Blot) (Western Blot analysis in transfected 293T cell line using 134604. Lane 1: 134604 transfected lysate (16.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis in NIH/3T3 using 134604.)
WB (Western Blot) (Western Blot analysis of in PC-12 using 134604.)
WB (Western Blot) (Western Blot analysis in HeLa using 134604.)
Application Data (Detection limit for recombinant GST tagged S100A4 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A4 on HeLa cell. [antibody concentration 15ug/ml])
WB (Western Blot) (Western Blot analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody. Lane 1: S100A4 transfected lysate (11.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (S100A4 monoclonal antibody Western Blot analysis of S100A4 expression in NIH/3T3.)
WB (Western Blot) (S100A4 monoclonal antibody Western Blot analysis of S100A4 expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (37.22kD))
WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])
WB (Western Blot) (Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (87.56kD).)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562 (Cat # L009V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining TCF7L2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TCF7L2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TCF7L2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TCF7L2 on Jurkat cells lysates using anti-TCF7L2 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with LOXL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining LOXL2 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining LOXL2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-LOXL2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of LOXL2 on PC-12 and A549 cell lysates using anti-LOXL2 antibody at 1/500 dilution.)
SDS-PAGE (Mouse lung whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TTF1(ABT237) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (MKRN2 monoclonal antibody Western Blot analysis of MKRN2 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MKRN2 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MKRN2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody.Lane 1: MKRN2 transfected lysate (46.9kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IF (Immunofluorescence) (Immunofluorescence analysis of Rat-lung tissue. 1,CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-colon tissue. 1,CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in Raw 264.7.)
WB (Western Blot) (CMAS monoclonal antibody, Western Blot analysis of CMAS expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged CMAS is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml].)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)
WB (Western Blot) (TIMM9 monoclonal antibody Western Blot analysis of TIMM9 expression in IMR-32.)
Application Data (Detection limit for recombinant GST tagged TIMM9 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMM9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TIMM9 expression in transfected 293T cell line by TIMM9 monoclonal antibody. Lane 1: TIMM9 transfected lysate (10.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M04), clone 2F4 Western Blot analysis of CSE1L expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml])
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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