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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 950-1000 of 10000 product results
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31351)
Full Name
Phospho-Histone H2B (Tyr43) Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Caspase 3 Antibody for IHC in human colon tissue)
Caspase 3, Polyclonal Antibody (Cat# AAA31093)
Full Name
Caspase 3 Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
p53, Polyclonal Antibody (Cat# AAA28938)
Full Name
p53 (phospho Ser15) Polyclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
ILK, Polyclonal Antibody (Cat# AAA29048)
Full Name
ILK Polyclonal Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
11beta-HSD1, Polyclonal Antibody (Cat# AAA29298)
Full Name
11beta-HSD1 Polyclonal Antibody
Gene Names
HSD11B1; HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Synapsin I, Polyclonal Antibody (Cat# AAA29127)
Full Name
Synapsin I Polyclonal Antibody
Gene Names
SYN1; SYNI; SYN1a; SYN1b
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 μg/ml.Green: ATF6 Antibody (3681)Blue: DAPI staining)
ATF6, Polyclonal Antibody (Cat# AAA10957)
Full Name
ATF6 Antibody
Gene Names
ATF6; ATF6A
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ATF6 Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Raji cell lysate; Lane3: NIH/3T3 cell lysate; Lane4: RAW264.7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Sjogren Syndrome Antigen B (SSB), Polyclonal Antibody (Cat# AAA21267)
Full Name
Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB)
Gene Names
SSB; La; LARP3
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of rat kidney cells using SLC5A2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
SLC5A2, Polyclonal Antibody (Cat# AAA22311)
Full Name
SLC5A2 Polyclonal Antibody
Gene Names
SLC5A2; SGLT2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)
DNMT1, Polyclonal Antibody (Cat# AAA30648)
Full Name
DNMT1 Rabbit Polyclonal Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse liver using HMGCS2 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)
HMGCS2, Polyclonal Antibody (Cat# AAA30568)
Full Name
HMGCS2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
p300, Polyclonal Antibody (Cat# AAA29317)
Full Name
p300 Polyclonal Antibody
Gene Names
EP300; p300; KAT3B; RSTS2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
NFAT2, Polyclonal Antibody (Cat# AAA31428)
Full Name
Phospho-NFAT2 (Ser172) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Nucleophosmin, Polyclonal Antibody (Cat# AAA31308)
Full Name
Phospho-Nucleophosmin (Ser125) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Jak2, Polyclonal Antibody (Cat# AAA31311)
Full Name
Phospho-Jak2 (Tyr1008) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of SiHa cells using anti-POR antibody (AAA11661).Overlay histogram showing SiHa cells stained with AAA11661 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POR Antibody (AAA11661,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
POR, Polyclonal Antibody (Cat# AAA11661)
Full Name
Anti-POR Antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of SARS-CoV-2 spike S1 recombinant protein probed with Rabbit anti SARS-CoV-2 spike protein RBD antibody (Lane 1: 2.5ug/ml, Lane 2: 5ug/ml, AAA12292). Arrow points to SARS-CoV-2 spike protein.)
COVID 19 Spike Protein Rbd Coronavirus, Polyclonal Antibody (Cat# AAA12292)
Full Name
Rabbit anti SARS-CoV-2 Spike Protein Rbd
Reactivity
Viral
Applications
Immunofluorescence, Western Blot
Purity
Rabbit polyclonal antibody purified by affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of FOXP1 using anti-FOXP1 antibody (AAA19227).FOXP1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FOXP1 Antibody (AAA19227) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
FOXP1, Polyclonal Antibody (Cat# AAA19227)
Full Name
Anti-FOXP1 Antibody
Gene Names
FOXP1; QRF1; 12CC4; hFKH1B; HSPC215
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-BECN1 Antibody Titration: 1 ug/mlPositive Control: HepG2 cell lysate)
BECN1, Polyclonal Antibody (Cat# AAA23584)
Full Name
BECN1 antibody - N-terminal region
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat
Applications
Western Blot
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Bcl-2, Polyclonal Antibody (Cat# AAA22320)
Full Name
(KO Validated) Bcl-2 Polyclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using AIMP1 antibody. Blue: DAPI for nuclear staining.)
AIMP1, Polyclonal Antibody (Cat# AAA28108)
Full Name
AIMP1 Polyclonal Antibody
Gene Names
AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using PARP1 antibody.)
PARP1, Polyclonal Antibody (Cat# AAA29790)
Full Name
PARP1 Polyclonal Antibody
Gene Names
PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using HuR/ ELAVL1 antibody.)
HuR/ELAVL1, Polyclonal Antibody (Cat# AAA29830)
Full Name
HuR/ELAVL1 Polyclonal Antibody
Gene Names
ELAVL1; HUR; Hua; MelG; ELAV1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using SPAM1 antibody. Blue: DAPI for nuclear staining.)
SPAM1, Polyclonal Antibody (Cat# AAA29645)
Full Name
SPAM1 Antibody
Gene Names
SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
ERK1/2, Polyclonal Antibody (Cat# AAA31300)
Full Name
Phospho-ERK1/2 (Thr177/Thr160) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
JAK1, Polyclonal Antibody (Cat# AAA29052)
Full Name
JAK1 Polyclonal Antibody
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Human gastric using Lamin A/C antibody at dilution of 1:100 (40x lens).)
LMNA, Polyclonal Antibody (Cat# AAA10641)
Full Name
LMNA Polyclonal Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
Microtubule Associated Protein 2 (MAP2), Polyclonal Antibody (Cat# AAA14235)
Full Name
Anti-Microtubule Associated Protein 2 (MAP2)
Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Bovine, Human, Mouse, Rat.
Applications
Western Blot, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA26885)
Full Name
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
Eomes; Tbr2; TBR-2; C77258
Reactivity
Mouse, Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Pricing
WB (Western Blot)
(Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD)
MARCH7, Polyclonal Antibody (Cat# AAA28826)
Full Name
MARCH7 Polyclonal Antibody
Gene Names
MARCH7; AXO; AXOT; RNF177; MARCH-VII
Reactivity
Rat, Pig, Dog, Cow
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
Lamin B1, Polyclonal Antibody (Cat# AAA29060)
Full Name
Lamin B1 Polyclonal Antibody
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
FoxO1, Polyclonal Antibody (Cat# AAA29026)
Full Name
FoxO1 Polyclonal Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
RSK1/2/3/4, Polyclonal Antibody (Cat# AAA31315)
Full Name
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Niban, Polyclonal Antibody (Cat# AAA31335)
Full Name
Niban Antibody
Gene Names
FAM129A; GIG39; NIBAN; C1orf24
Reactivity
Human, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
CREB, Polyclonal Antibody (Cat# AAA31303)
Full Name
Phospho-CREB (Ser129) Antibody
Gene Names
CREB1; CREB
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
S100A9, Polyclonal Antibody (Cat# AAA31466)
Full Name
Phospho-S100A9 (Thr113) Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300 IHC 1:50-300)
JNK1/2/3, Polyclonal Antibody (Cat# AAA28934)
Full Name
JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat, Chicken
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)
Stat3, Polyclonal Antibody (Cat# AAA28941)
Full Name
Stat3 (phospho Ser727) Polyclonal Antibody
Gene Names
STAT3; APRF; HIES
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
GTBP, Polyclonal Antibody (Cat# AAA29034)
Full Name
GTBP Polyclonal Antibody
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human, Mouse, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Rab 5A, Polyclonal Antibody (Cat# AAA29261)
Full Name
Rab 5A Polyclonal Antibody
Gene Names
RAB5A; RAB5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of RH35 cells using anti-Tropomyosin 2/TPM2 antibody (AAA19476).Overlay histogram showing RH35 cells stained with AAA19476 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tropomyosin 2/TPM2 Antibody (AAA19476, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Tropomyosin 2/TPM2, Polyclonal Antibody (Cat# AAA19476)
Full Name
Anti-Tropomyosin 2/TPM2 Antibody Picoband
Gene Names
TPM2; DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 600ug extracts of Mouse brain usingug N-Cadherin Polyclonal Antibody. Western blot was performed from the immunoprecipitate using N-Cadherin Polyclonal Antibody at a dilution of 1:1000.)
N-Cadherin, Polyclonal Antibody (Cat# AAA22314)
Full Name
(KO Validated) N-Cadherin Polyclonal Antibody
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200)
pS2, Polyclonal Antibody (Cat# AAA30757)
Full Name
pS2 Rabbit Polyclonal Antibody
Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Reactivity
Human, Rat, Mouse
Applications
Immunohistochemistry, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-GBP1 antibody (AAA19787).Overlay histogram showing MCF-7 cells stained with AAA19787 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GBP1 Antibody (AAA19787, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GBP1, Polyclonal Antibody (Cat# AAA19787)
Full Name
Anti-GBP1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Anti-CRFR1 / CRHR1 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
CRFR1 / CRHR1, Polyclonal Antibody (Cat# AAA12338)
Full Name
Rabbit Polyclonal to Human CRFR1 / CRHR1
Gene Names
CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
Reactivity
Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat
Predicted Reactivity: Bat, Bovine, Horse (at least 90% immunogen sequence identity)
Predicted Reactivity: Bat, Bovine, Horse (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry-Phospho-PEA15-S104 pAb)
PEA15-S104, Polyclonal Antibody (Cat# AAA28302)
Full Name
Phospho-PEA15-S104 pAb
Gene Names
PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000)
Perforin 1, Polyclonal Antibody (Cat# AAA29334)
Full Name
Perforin 1 Polyclonal Antibody
Gene Names
PRF1; P1; PFP; FLH2; PFN1; HPLH2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
HSPBAP1, Polyclonal Antibody (Cat# AAA29153)
Full Name
HSPBAP1 Polyclonal Antibody
Gene Names
HSPBAP1; PASS1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
TDP43, Polyclonal Antibody (Cat# AAA31323)
Full Name
Phospho-TDP43 (Ser409/Ser410) Antibody
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
HP1 alpha, Polyclonal Antibody (Cat# AAA31332)
Full Name
Phospho-HP1 alpha (Ser92) Antibody
Gene Names
CBX5; HP1; HP1A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.