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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 9250-9300 of 96812 product results
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of U937 cells using anti-BHMT antibody (AAA125778).Overlay histogram showing U937 cells stained with AAA125778 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BHMT Antibody (AAA125778, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BHMT, Polyclonal Antibody (Cat# AAA125778)
Full Name
Anti-BHMT Antibody
Gene Names
BHMT; BHMT1; HEL-S-61p
Reactivity
Human, Mouse, Rat, Monkey
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEPG2 cells using anti-Sema6A antibody (AAA125781).Overlay histogram showing HEPG2 cells stained with AAA125781 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sema6A Antibody (AAA125781, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Sema6A, Polyclonal Antibody (Cat# AAA125781)
Full Name
Anti-Sema6A Antibody
Gene Names
SEMA6A; VIA; SEMA; HT018; SEMAQ; SEMA6A1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of PSME2 using anti-PSME2 antibody (AAA125785).PSME2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-PSME2 Antibody (AAA125785) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PSME2, Polyclonal Antibody (Cat# AAA125785)
Full Name
Anti-PSME2 Antibody
Gene Names
PSME2; PA28B; REGbeta; PA28beta
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of THP-1 cells using anti-METTL14 antibody (AAA125790).Overlay histogram showing THP-1 cells stained with AAA125790 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-METTL14 Antibody (AAA125790, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
METTL14, Polyclonal Antibody (Cat# AAA125790)
Full Name
Anti-METTL14 Antibody
Gene Names
METTL14; hMETTL14
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RH35 cells using anti-Tspan1 antibody (AAA125799).Overlay histogram showing RH35 cells stained with AAA125799 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tspan1 Antibody (AAA125799, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Tspan1, Polyclonal Antibody (Cat# AAA125799)
Full Name
Anti-Tspan1 Antibody
Reactivity
Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of 293T cells using anti-Apc5/ANAPC5 antibody (AAA125800).Overlay histogram showing 293T cells stained with AAA125800 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apc5/ANAPC5 Antibody (AAA125800, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Apc5/ANAPC5, Polyclonal Antibody (Cat# AAA125800)
Full Name
Anti-Apc5/ANAPC5 Antibody
Gene Names
ANAPC5; APC5
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HL-60 cells using anti-UPF3A antibody (AAA125801).Overlay histogram showing HL-60 cells stained with AAA125801 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UPF3A Antibody (AAA125801, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
UPF3A, Polyclonal Antibody (Cat# AAA125801)
Full Name
Anti-UPF3A Antibody
Gene Names
UPF3A; UPF3; HUPF3A; RENT3A
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 3. IF analysis of MAP4K5 using anti- MAP4K5 antibody (AAA125810).MAP4K5 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- MAP4K5 Antibody (AAA125810) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
MAP4K5, Polyclonal Antibody (Cat# AAA125810)
Full Name
Anti-MAP4K5 Antibody
Gene Names
MAP4K5; KHS; GCKR; KHS1; MAPKKKK5
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Alas1 using anti-Alas1 antibody (AAA125811).Alas1 was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Alas1 Antibody (AAA125811) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Alas1, Polyclonal Antibody (Cat# AAA125811)
Full Name
Anti-Alas1 Antibody
Gene Names
ALAS1; ALAS; MIG4; ALAS3; ALASH
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of A431 cells using anti-NDUFB3 antibody (AAA125817).Overlay histogram showing A431 cells stained with AAA125817 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB3 Antibody (AAA125817, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFB3, Polyclonal Antibody (Cat# AAA125817)
Full Name
Anti-NDUFB3 Antibody
Gene Names
NDUFB3; B12; CI-B12
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of COX8A using anti-COX8A antibody (AAA125818).COX8A was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-COX8A Antibody (AAA125818) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
COX8A, Polyclonal Antibody (Cat# AAA125818)
Full Name
Anti-COX8A Antibody
Gene Names
Cox8a; COX8L
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of THP-1 cells using anti-HYI antibody (AAA125821).Overlay histogram showing THP-1 cells stained with AAA125821 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HYI Antibody (AAA125821, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HYI, Polyclonal Antibody (Cat# AAA125821)
Full Name
Anti-HYI Antibody
Gene Names
HYI; HT036
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U20S cells using anti-SHISA6 antibody (AAA125822).Overlay histogram showing U20S cells stained with AAA125822 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHISA6 Antibody (AAA125822, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SHISA6, Polyclonal Antibody (Cat# AAA125822)
Full Name
Anti-SHISA6 Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of HEPA1-6 cells using anti-Cox6a2 antibody (AAA125823).Overlay histogram showing HEPA1-6 cells stained with AAA125823 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cox6a2 Antibody (AAA125823, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Cox6a2, Polyclonal Antibody (Cat# AAA125823)
Full Name
Anti-Cox6a2 Antibody
Gene Names
Cox6a2; VIaH; CoxVIaH
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
mTOR/MTOR, Polyclonal Antibody (Cat# AAA125167)
Full Name
Anti-mTOR/MTOR Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Monkey
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-C3 antibody (AAA125523).Overlay histogram showing 293T cells stained with AAA125523 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C3 Antibody (AAA125523, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
C3, Polyclonal Antibody (Cat# AAA125523)
Full Name
Anti-C3 Antibody
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of Icam1 using anti-Icam1 antibody (AAA125524).Icam1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Icam1 Antibody (AAA125524) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Icam1, Polyclonal Antibody (Cat# AAA125524)
Full Name
Anti-Icam1 Antibody
Gene Names
Icam1; CD54; Ly-47; Icam-1; MALA-2
Reactivity
Mouse
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of U20S cells using anti-NCAM1 antibody (AAA125525).Overlay histogram showing U20S cells stained with AAA125525 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NCAM1 Antibody (AAA125525, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NCAM1, Polyclonal Antibody (Cat# AAA125525)
Full Name
Anti-NCAM1 Antibody
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Ki67/Mki67 using anti-Ki67/Mki67 antibody (AAA125531).Ki67/Mki67 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ki67/Mki67 Antibody (AAA125531) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Ki67/Mki67, Polyclonal Antibody (Cat# AAA125531)
Full Name
Anti-Ki67/Mki67 Antibody
Gene Names
Mki67; Ki67; Ki-67; D630048A14Rik
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-c-Fos/FOS antibody (AAA125538).Overlay histogram showing SiHa cells stained with AAA125538 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Fos/FOS Antibody (AAA125538, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
c-Fos/FOS, Polyclonal Antibody (Cat# AAA125538)
Full Name
Anti-c-Fos/FOS Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 2. IF analysis of IL2 Receptor gamma/IL2RG using anti- IL2 Receptor gamma/IL2RG antibody (AAA125540).IL2 Receptor gamma/IL2RG was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- IL2 Receptor gamma/IL2RG Antibody (AAA125540) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IL2 Receptor gamma/IL2RG, Polyclonal Antibody (Cat# AAA125540)
Full Name
Anti-IL2 Receptor gamma/IL2RG Antibody
Gene Names
IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-CD11c/Itgax antibody (AAA125543).Overlay histogram showing mouse spleen tissues stained with AAA125543 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11c/Itgax Antibody (AAA125543, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD11c/Itgax, Polyclonal Antibody (Cat# AAA125543)
Full Name
Anti-CD11c/Itgax Antibody
Gene Names
Itgax; Cr4; N418; Cd11c; AI449405
Reactivity
Mouse
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of RAW264. 7 cells using anti-TMS1/ASC/PYCARD antibody (AAA125544).Overlay histogram showing RAW264. 7 cells stained with AAA125544 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMS1/ASC/PYCARD Antibody (AAA125544, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TMS1/ASC/Pycard, Polyclonal Antibody (Cat# AAA125544)
Full Name
Anti-TMS1/ASC/Pycard Antibody
Gene Names
Pycard; Asc; TNS1; masc; CARD5; TMS-1; 9130417A21Rik
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of RH35 cells using anti-TIMP1 antibody (AAA125559).Overlay histogram showing RH35 cells stained with AAA125559 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMP1 Antibody (AAA125559, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TIMP1, Polyclonal Antibody (Cat# AAA125559)
Full Name
Anti-TIMP1 Antibody
Gene Names
Timp1; EPA; Clgi; Timp; TIMP-1; TPA-S1
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of Cd2 using anti-Cd2 antibody (AAA125561).Cd2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Cd2 Antibody (AAA125561) overnight at 4 degree C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Cd2, Polyclonal Antibody (Cat# AAA125561)
Full Name
Anti-Cd2 Antibody
Gene Names
Cd2; CD2R; LFA2; OX34; LFA-2; OX-34
Reactivity
Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of U937 cells using anti-POT1 antibody (AAA125569).Overlay histogram showing U937 cells stained with AAA125569 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POT1 Antibody (AAA125569, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
POT1, Polyclonal Antibody (Cat# AAA125569)
Full Name
Anti-POT1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 4. IF analysis of ARF6 using anti- ARF6 antibody (AAA125580).ARF6 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- ARF6 Antibody (AAA125580) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
ARF6, Polyclonal Antibody (Cat# AAA125580)
Full Name
Anti-ARF6 Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-AMPK alpha 1/PRKAA1 antibody (AAA125582).Overlay histogram showing SiHa cells stained with AAA125582 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK alpha 1/PRKAA1 Antibody (AAA125582, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AMPK alpha 1/PRKAA1, Polyclonal Antibody (Cat# AAA125582)
Full Name
Anti-AMPK alpha 1/PRKAA1 Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Integrin beta 4/ITGB4 using anti-Integrin beta 4/ITGB4 antibody (AAA125585).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human A549 whole cell lysatesLane 2: human PC-3 whole cell lysatesLane 3: human T-47D whole cell lysatesLane 4: human Hela whole cell lysatesLane 5: human SW620 whole cell lysatesLane 6: human CACO-2 whole cell lysatesLane 7: rat ovary tissue lysatesLane 8: mouse ovary tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin beta 4/ITGB4 antigen affinity purified polyclonal antibody (Catalog # AAA125585) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Integrin beta 4/ITGB4 at approximately 210KD. The expected band size for Integrin beta 4/ITGB4 is at 210KD.)
Integrin beta 4/ITGB4, Polyclonal Antibody (Cat# AAA125585)
Full Name
Anti-Integrin beta 4/ITGB4 Antibody
Gene Names
ITGB4; CD104
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of Cd63 using anti-Cd63 antibody (AAA125588).Cd63 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cd63 Antibody (AAA125588) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cd63, Polyclonal Antibody (Cat# AAA125588)
Full Name
Anti-Cd63 Antibody
Gene Names
Cd63; ME491; C75951; Tspan30
Reactivity
Mouse, Rat
Applications
Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SiHa cells using anti-TSG101 antibody (AAA125596).Overlay histogram showing SiHa cells stained with AAA125596 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TSG101 Antibody (AAA125596, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TSG101, Polyclonal Antibody (Cat# AAA125596)
Full Name
Anti-TSG101 Antibody
Gene Names
TSG101; TSG10; VPS23
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
SUMO2/3, Polyclonal Antibody (Cat# AAA125601)
Full Name
Anti-SUMO2/3 Antibody
Gene Names
SUMO2; HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of SUFU using anti-SUFU antibody.)
SUFU, Polyclonal Antibody (Cat# AAA125448)
Full Name
Anti-SUFU Antibody
Gene Names
SUFU; SUFUH; JBTS32; SUFUXL; PRO1280
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PLD2 using anti-PLD2 antibody.)
PLD2, Polyclonal Antibody (Cat# AAA125451)
Full Name
Anti-PLD2 Antibody
Gene Names
PLD2; PLD1C
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PPARGC1B using anti-PPARGC1B antibody.)
PGC1 beta/PPARGC1B, Polyclonal Antibody (Cat# AAA125458)
Full Name
Anti-PGC1 beta/PPARGC1B Antibody
Gene Names
PPARGC1B; PERC; ERRL1; PGC1B; PGC-1(beta)
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of OFD1 using anti-OFD1 antibody.)
OFD1, Polyclonal Antibody (Cat# AAA125459)
Full Name
Anti-OFD1 Antibody
Gene Names
OFD1; RP23; 71-7A; SGBS2; CXorf5; JBTS10
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of SLBP using anti-SLBP antibody.)
SLBP, Polyclonal Antibody (Cat# AAA125460)
Full Name
Anti-SLBP Antibody
Gene Names
SLBP; HBP
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of WNT10A using anti-WNT10A antibody.)
Wnt10a, Polyclonal Antibody (Cat# AAA125467)
Full Name
Anti-Wnt10a Antibody
Gene Names
WNT10A; OODD; SSPS; STHAG4
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
SCN11A, Polyclonal Antibody (Cat# AAA125474)
Full Name
Anti-SCN11A Antibody
Gene Names
SCN11A; NaN; PN5; FEPS3; HSAN7; SNS-2; NAV1.9; SCN12A
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of IKZF2 using anti-IKZF2 antibody.)
ZNFN1A2/HELIOS/IKZF2, Polyclonal Antibody (Cat# AAA125478)
Full Name
Anti-ZNFN1A2/HELIOS/IKZF2 Antibody
Gene Names
IKZF2; ANF1A2; HELIOS; ZNF1A2; ZNFN1A2
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of MORC3 using anti-MORC3 antibody.)
MORC3, Polyclonal Antibody (Cat# AAA125482)
Full Name
Anti-MORC3 Antibody
Gene Names
MORC3; NXP2; ZCW5; ZCWCC3
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of FOXI1 using anti-FOXI1 antibody.)
FOXI1, Polyclonal Antibody (Cat# AAA125490)
Full Name
Anti-FOXI1 Antibody
Gene Names
FOXI1; HFH3; FKH10; HFH-3; FKHL10; FREAC6; FREAC-6
Reactivity
Human, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Raw264. 7 cells using anti-TNF Alpha/Tnf antibody (AAA125508).Overlay histogram showing Raw264. 7 cells stained with AAA125508 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TNF Alpha/Tnf Antibody (AAA125508, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TNF alpha/Tnf, Polyclonal Antibody (Cat# AAA125508)
Full Name
Anti-TNF alpha/Tnf Antibody
Gene Names
Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (AAA125512).Overlay histogram showing THP-1 cells stained with AAA125512 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (AAA125512, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CIAS1/NALP3/NLRP3, Polyclonal Antibody (Cat# AAA125512)
Full Name
Anti-CIAS1/NALP3/NLRP3 Antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of 293T cells using anti-NFE2L2 antibody (AAA125514).Overlay histogram showing 293T cells stained with AAA125514 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFE2L2 Antibody (AAA125514, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NFE2L2, Polyclonal Antibody (Cat# AAA125514)
Full Name
Anti-NFE2L2 Antibody
Gene Names
NFE2L2; NRF2
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of 293T cells using anti-BRD4 antibody (AAA125518).Overlay histogram showing 293T cells stained with AAA125518 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRD4 Antibody (AAA125518, 1 μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BRD4, Polyclonal Antibody (Cat# AAA125518)
Full Name
Anti-BRD4 Antibody
Gene Names
BRD4; CAP; MCAP; HUNK1; HUNKI
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of K562 cells using anti-IRF3 antibody (AAA125522).Overlay histogram showing K562 cells stained with AAA125522 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF3 Antibody (AAA125522, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IRF3, Polyclonal Antibody (Cat# AAA125522)
Full Name
Anti-IRF3 Antibody
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
MCK10/NEP/DDR1, Polyclonal Antibody (Cat# AAA125378)
Full Name
Anti-MCK10/NEP/DDR1 Antibody
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of Egfr using anti-Egfr antibody.)
EGFR, Polyclonal Antibody (Cat# AAA125387)
Full Name
Anti-EGFR Antibody
Gene Names
Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of PTGS2 using anti-PTGS2 antibody.)
COX2/Cyclooxygenase 2/PTGS2, Polyclonal Antibody (Cat# AAA125390)
Full Name
Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody
Gene Names
PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.
As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.