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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1250-1300 of 3176 product results
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Integrin alphaV, Polyclonal Antibody (Cat# AAA29049)
Full Name
Integrin alphaV Polyclonal Antibody
Gene Names
ITGAV; CD51; MSK8; VNRA; VTNR
Reactivity
Human, Mouse
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry-NUBP1 Polyclonal Antibody)
NUBP1, Polyclonal Antibody (Cat# AAA28296)
Full Name
NUBP1 Polyclonal Antibody
Gene Names
NUBP1; NBP; NBP1; CIAO5; NBP35
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of SiHa cells using anti-SF3A2 antibody (AAA19955).Overlay histogram showing SiHa cells stained with AAA19955 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SF3A2 Antibody (AAA19955, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SF3A2, Polyclonal Antibody (Cat# AAA19955)
Full Name
Anti-SF3A2 Antibody Picoband
Gene Names
SF3A2; PRP11; SAP62; PRPF11; SF3a66
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Rat Brain Tissue)
Microtubule Associated Protein 4, Polyclonal Antibody (Cat# AAA20859)
Full Name
Polyclonal Antibody to Microtubule Associated Protein 4 (MAP4)
Gene Names
Map4; Mtap4
Reactivity
Human, Rat
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
IHC (Immunohistochemistry)
(Figure 14. IHC analysis of FABP-1/GOT2 using anti-FABP-1/GOT2 antibody (AAA19309).FABP-1/GOT2 was detected in paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-FABP-1/GOT2 Antibody (AAA19309) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
FABP-1/GOT2, Polyclonal Antibody (Cat# AAA19309)
Full Name
Anti-FABP-1/GOT2 Antibody
Gene Names
GOT2; KAT4; KATIV; mitAAT
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat pancreas using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IRE1, Polyclonal Antibody (Cat# AAA22308)
Full Name
IRE1 Polyclonal Antibody
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200)
pS2, Polyclonal Antibody (Cat# AAA30757)
Full Name
pS2 Rabbit Polyclonal Antibody
Gene Names
TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
Reactivity
Human, Rat, Mouse
Applications
IHC-P, IF, paraffin section, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PAK5/6, Polyclonal Antibody (Cat# AAA31281)
Full Name
Phospho-PAK5/6 (Ser602/Ser560) Antibody
Gene Names
PAK7; PAK5
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Blank control: Molt-4 Cells(blue). Primary Antibody: Rabbit Anti-EPOR/FITC Conjugated antibody, Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG orange,used under the same conditions. Protocol: The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.)
EPOR, Polyclonal Antibody (Cat# AAA27968)
Full Name
EPOR
Reactivity
Human, Mouse, Rat, Dog, Cow, Horse
Applications
IF, WB, IHC, FC, EIA
Purity
Affinity purified by Protein A
Pricing
IHC (Immunohistochemistry)
(GCC2 antibody (C-term) (AAA28751) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GCC2 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
GCC2, Polyclonal Antibody (Cat# AAA28751)
Full Name
GCC2 Antibody (C-term)
Gene Names
GCC2; REN53; GCC185; RANBP2L4
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
WB, EIA, IHC-P
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Integrin alphaD, Polyclonal Antibody (Cat# AAA29263)
Full Name
Integrin alphaD Polyclonal Antibody
Gene Names
ITGAD; ADB2; CD11D
Reactivity
Human
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Jak2, Polyclonal Antibody (Cat# AAA31311)
Full Name
Phospho-Jak2 (Tyr1008) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using CD274 antibody. Blue: DAPI for nuclear staining.)
CD274, Polyclonal Antibody (Cat# AAA28238)
Full Name
CD274 Polyclonal Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)
IGFBP3, Polyclonal Antibody (Cat# AAA29283)
Full Name
IGFBP3 Polyclonal Antibody
Gene Names
IGFBP3; IBP3; BP-53
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Lamin A/C, Polyclonal Antibody (Cat# AAA29059)
Full Name
Lamin A/C Polyclonal Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
Application Data
NMDA NR2A Subunit, Polyclonal Antibody (Cat# AAA14232)
Full Name
Anti-NMDA Receptor, NR2A Subunit
Gene Names
Grin2a; NR2A; GluN2A; NMDAR2A
Reactivity
Rat, mouse, human
Applications
WB, IHC, IP
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Jurkat cells using anti-SWAP70 antibody (AAA19880).Overlay histogram showing Jurkat cells stained with AAA19880 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SWAP70 Antibody (AAA19880, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SWAP70, Polyclonal Antibody (Cat# AAA19880)
Full Name
Anti-SWAP70 Antibody Picoband
Gene Names
SWAP70; HSPC321; SWAP-70
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(ZEB2 antibody - middle region validated by WB using H9 human embryonic stem cells at 1:1000.)
ZEB2, Polyclonal Antibody (Cat# AAA23465)
Full Name
ZEB2 antibody - middle region
Gene Names
ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
Reactivity
Cow, Guinea Pig, Horse, Human, Mouse, Pig, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
GTBP, Polyclonal Antibody (Cat# AAA29034)
Full Name
GTBP Polyclonal Antibody
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human, Mouse, Monkey
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
ILK, Polyclonal Antibody (Cat# AAA29048)
Full Name
ILK Polyclonal Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using Filamin A antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Filamin A, Polyclonal Antibody (Cat# AAA28331)
Full Name
Filamin A Rabbit pAb
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)
DNMT1, Polyclonal Antibody (Cat# AAA30648)
Full Name
DNMT1 Rabbit Polyclonal Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of K562 cells using anti-MOCS1 antibody (AAA19891).Overlay histogram showing K562 cells stained with AAA19891 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOCS1 Antibody (AAA19891, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MOCS1, Polyclonal Antibody (Cat# AAA19891)
Full Name
Anti-MOCS1 Antibody Picoband
Gene Names
MOCS1; MIG11; MOCOD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescence analysis of HeLa cells using LMNB1 at dilution of 1:200. Blue: DAPI for nuclear staining.)
LMNB1, Polyclonal Antibody (Cat# AAA30657)
Full Name
LMNB1 Rabbit Polyclonal Antibody
Gene Names
LMNB1; LMN; ADLD; LMN2; LMNB
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HL-60 cells using anti-RPS15A antibody (AAA19921).Overlay histogram showing HL-60 cells stained with AAA19921 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS15A Antibody (AAA19921, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPS15A, Polyclonal Antibody (Cat# AAA19921)
Full Name
Anti-RPS15A Antibody Picoband
Gene Names
RPS15A; S15a
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19170).Annexin VI was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Annexin VI Antibody (AAA19170) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Annexin VI, Polyclonal Antibody (Cat# AAA19170)
Full Name
Anti-Annexin VI Picoband Antibody
Gene Names
ANXA6; ANX6; CBP68
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HEL cells using anti-LYPLA2 antibody (AAA19947).Overlay histogram showing HEL cells stained with AAA19947 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LYPLA2 Antibody (AAA19947, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
LYPLA2, Polyclonal Antibody (Cat# AAA19947)
Full Name
Anti-LYPLA2 Antibody Picoband
Gene Names
LYPLA2; APT2; APT-2; DJ886K2.4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HL-60 cells using anti-Sumo 1/SUMO1 antibody (AAA19224).Overlay histogram showing HL-60 cells stained with AAA19224 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sumo 1/SUMO1 Antibody (AAA19224, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Sumo 1/SUMO1, Polyclonal Antibody (Cat# AAA19224)
Full Name
Anti-Sumo 1/SUMO1 Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human, Mouse, Rat
Applications
IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 10. Western blot analysis of KHSRP using anti-KHSRP antibody (AAA19468).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat stomach tissue lysates,Lane 3: mouse brain tissue lysates,Lane 4: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KHSRP antigen affinity purified polyclonal antibody (#AAA19468) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KHSRP at approximately 85-90 kDa. The expected band size for KHSRP is at 73 kDa.)
KHSRP, Polyclonal Antibody (Cat# AAA19468)
Full Name
Anti-KHSRP Antibody Picoband
Gene Names
KHSRP; FBP2; KSRP; FUBP2
Reactivity
Human, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of K562 cells using anti-COX6B1 antibody (AAA19617).Overlay histogram showing K562 cells stained with AAA19617 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX6B1 Antibody (AAA19617, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
COX6B1, Polyclonal Antibody (Cat# AAA19617)
Full Name
Anti-COX6B1 Antibody Picoband
Gene Names
COX6B1; COXG; COX6B; COXVIb1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-SSB antibody (AAA19405).Overlay histogram showing A431 cells stained with AAA19405 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSB Antibody (AAA19405, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SSB, Polyclonal Antibody (Cat# AAA19405)
Full Name
Anti-SSB Antibody Picoband
Gene Names
SSB; La; LARP3
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
MK, Polyclonal Antibody (Cat# AAA29166)
Full Name
MK Polyclonal Antibody
Gene Names
MDK; MK; ARAP; NEGF2
Reactivity
Human, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
Acid Phosphatase 3, Prostatic (ACP3), Polyclonal Antibody (Cat# AAA20146)
Full Name
Polyclonal Antibody to Acid Phosphatase 3, Prostatic (ACP3)
Gene Names
ACPP; ACP3; 5'-NT; ACP-3
Reactivity
Human
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
WB (Western Blot)
(Western Blot;Sample: Rat Heart Tissue.)
Myosin Light Chain 4, Alkali, Atrial, Embryonic (MYL4), Polyclonal Antibody (Cat# AAA20148)
Full Name
Polyclonal Antibody to Myosin Light Chain 4, Alkali, Atrial, Embryonic (MYL4)
Gene Names
Myl4; ELC; GT1; ALC1; AMLC; Myla; ELC1a; MLC1a; MLC1EMB
Reactivity
Mouse, Rat
Applications
WB, IHC, ICC, IP
Purity
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistchemistry)
(Figure 9. IHC analysis of ERBB4 using anti-ERBB4 antibody (AAA19729).ERBB4 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ERBB4 Antibody (AAA19729) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB4 antigen affinity purified polyclonal antibody (#AAA19729) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERBB4 at approximately 180 kDa. The expected band size for ERBB4 is at 147 kDa.)
ERBB4, Polyclonal Antibody (Cat# AAA19729)
Full Name
Anti-ERBB4 Antibody Picoband
Gene Names
ERBB4; HER4; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: K562 cell lysate; Lane4: MCF7 cell lysatePrimary Ab: 0.2ug/ml Rabbit Anti-Human PKM2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Pyruvate kinase isozymes M2 (PKM2), Polyclonal Antibody (Cat# AAA21265)
Full Name
Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
CREB, Polyclonal Antibody (Cat# AAA31303)
Full Name
Phospho-CREB (Ser129) Antibody
Gene Names
CREB1; CREB
Reactivity
Human, Mouse, Rat
Applications
IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using [KD Validated] eIF4E Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
eIF4E, Polyclonal Antibody (Cat# AAA22335)
Full Name
(KD Validated) eIF4E Polyclonal Antibody
Gene Names
EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
SNAIL, Polyclonal Antibody (Cat# AAA31310)
Full Name
Phospho-SNAIL (Ser11) Antibody
Gene Names
SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
RSK1/2/3/4, Polyclonal Antibody (Cat# AAA31315)
Full Name
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using CDKN1A antibody.)
CDKN1A, Polyclonal Antibody (Cat# AAA28244)
Full Name
CDKN1A Polyclonal Antibody
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2B, Polyclonal Antibody (Cat# AAA31351)
Full Name
Phospho-Histone H2B (Tyr43) Antibody
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Rabbit pAb (AAA28290) at dilution of 1:100 (40x lens).)
C9orf72, Polyclonal Antibody (Cat# AAA28290)
Full Name
C9orf72 Polyclonal Antibody
Gene Names
C9orf72; ALSFTD; DENND9; FTDALS; DENNL72; FTDALS1
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] HIF1?? Rabbit pAb.)
HIF1A, Polyclonal Antibody (Cat# AAA30597)
Full Name
HIF1A Rabbit Polyclonal Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-SUV420H1 AntibodyTitration: 1.25 ug/mlPositive Control: Jurkat Whole Cell)
KMT5B, Polyclonal Antibody (Cat# AAA23434)
Full Name
KMT5B Antibody - middle region
Gene Names
KMT5B; CGI85; MRD51; CGI-85; SUV420H1
Reactivity
Dog, Guinea Pig, Horse, Human, Mouse, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Mucin 16, Polyclonal Antibody (Cat# AAA29075)
Full Name
Mucin 16 Polyclonal Antibody
Gene Names
MUC16; CA125
Reactivity
Human
Applications
IF
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
N-cadherin, Polyclonal Antibody (Cat# AAA29079)
Full Name
N-cadherin Polyclonal Antibody
Gene Names
CDH2; CDHN; NCAD; CD325; CDw325
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-ADK antibody (AAA11690).Overlay histogram showing PC-3 cells stained with AAA11690 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADK Antibody (AAA11690,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ADK, Polyclonal Antibody (Cat# AAA11690)
Full Name
Anti-ADK Antibody
Gene Names
ADK; AK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of CX3CR1 in rat heart tissue with CX3CR1 antibody at 20 μg/ml.Green: CX3CR1 Antibody (2093)Blue: DAPI staining)
CX3CR1, Polyclonal Antibody (Cat# AAA10919)
Full Name
CX3CR1 Antibody
Gene Names
CX3CR1; V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
CX3CR1 Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Figure 9 KD Validation in HeLa Cells (Horinaka et al., 2005)HeLa cells were transfected with DR4siRNA or LacZ control siRNA. At 24 h after transfection, the cells were treated with or without 20 μM luteolin for 24 h. Western blot analysis was carried out with anti-DR4 antibodies. DR4 expression was markedly reduced after DR4 knockdown.)
DR4, Polyclonal Antibody (Cat# AAA10950)
Full Name
DR4 Antibody
Gene Names
TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
Reactivity
Human
Applications
EIA, WB
Purity
DR4 Antibody is affinity chromatography purified via peptide column.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.