Polyclonal Antibodies

JTB, Polyclonal Antibody (Cat# AAA17962)

Full Name

JTB Antibody

Gene Names

JTB; PAR; hJT; HJTB; HSPC222

Reactivity

Human
Predicted: Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 Polyclonal Antibody at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.)

Heme Oxygenase 1, Polyclonal Antibody (Cat# AAA22319)

Full Name

Heme Oxygenase 1 Polyclonal Antibody

Gene Names

HMOX1; HO-1; HSP32; HMOX1D; bK286B10

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

FOXN2, Polyclonal Antibody (Cat# AAA28719)

Full Name

FOXN2 Antibody (N-term)

Gene Names

FOXN2; HTLF

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA)

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Caspase-1, Polyclonal Antibody (Cat# AAA28975)

Full Name

Caspase-1 Polyclonal Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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Calsenilin/KCNIP3, Polyclonal Antibody (Cat# AAA31279)

Full Name

Phospho-Calsenilin/KCNIP3 (Ser63) Antibody

Gene Names

KCNIP3; CSEN; DREAM; KCHIP3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of MCF-7 cells using anti-DIS3 antibody (AAA19760).Overlay histogram showing MCF-7 cells stained with AAA19760 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DIS3 Antibody (AAA19760, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 10. IF analysis of DIS3 using anti-DIS3 antibody (AAA19760) and anti-Beta Tubulin antibody (M01857-3).DIS3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-DIS3 Antibody (AAA19760) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Raji whole cell lysates,Lane 3: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DIS3 antigen affinity purified polyclonal antibody (#AAA19760) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DIS3 at approximately 109 kDa. The expected band size for DIS3 is at 109 kDa.)

DIS3, Polyclonal Antibody (Cat# AAA19760)

Full Name

Anti-DIS3 Antibody Picoband

Gene Names

DIS3; RRP44; dis3p; EXOSC11; KIAA1008; 2810028N01Rik

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Lanes:Lane1: 400ug rat hippocampalLane2: 300ug rat hippocampalLane3: 200ug rat hippocampalLane4: 100ug rat hippocampalPrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:8000Gene Name:GPM6ASubmitted by:Anonymous)

GPM6A, Polyclonal Antibody (Cat# AAA23595)

Full Name

GPM6A antibody - C-terminal region

Gene Names

GPM6A; M6A; GPM6

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

p73, Polyclonal Antibody (Cat# AAA29227)

Full Name

p73 Polyclonal Antibody

Gene Names

TP73; P73

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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HDGF, Polyclonal Antibody (Cat# AAA30763)

Full Name

HDGF Rabbit Polyclonal Antibody

Gene Names

HDGF; HMG1L2

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Raw264.7 cells using anti-Lat antibody (AAA19756).Overlay histogram showing Raw264.7 cells stained with AAA19756 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Lat Antibody (AAA19756, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of Lat using anti-Lat antibody (AAA19756).Lat was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lat Antibody (AAA19756) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lat antigen affinity purified polyclonal antibody (#AAA19756) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lat at approximately 38 kDa. The expected band size for Lat is at 26 kDa.)

Lat, Polyclonal Antibody (Cat# AAA19756)

Full Name

Anti-Lat Antibody Picoband

Gene Names

Lat; pp36; p36-38

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using BMP5 antibody at dilution of 1:100 (40x lens).)

BMP5, Polyclonal Antibody (Cat# AAA28209)

Full Name

BMP5 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Anti-CRFR1 / CRHR1 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

CRFR1 / CRHR1, Polyclonal Antibody (Cat# AAA12338)

Full Name

Rabbit Polyclonal to Human CRFR1 / CRHR1

Gene Names

CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1

Reactivity

Gibbon, Gorilla, Hamster, Human, Monkey, Mouse, Rat
Predicted Reactivity: Bat, Bovine, Horse (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of RT4 cells using anti-RAD51C antibody (AAA19762).Overlay histogram showing RT4 cells stained with AAA19762 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAD51C Antibody (AAA19762, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RAD51C using anti-RAD51C antibody (AAA19762).RAD51C was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAD51C Antibody (AAA19762) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: rat testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD51C antigen affinity purified polyclonal antibody (#AAA19762) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAD51C at approximately 42 kDa. The expected band size for RAD51C is at 42 kDa.)

RAD51C, Polyclonal Antibody (Cat# AAA19762)

Full Name

Anti-RAD51C Antibody Picoband

Gene Names

RAD51C; FANCO; BROVCA3; RAD51L2

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody at dilution of 1:100 (40x lens).)

MDC1, Polyclonal Antibody (Cat# AAA28205)

Full Name

MDC1 Polyclonal Antibody

Gene Names

MDC1; NFBD1

Reactivity

Human

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-ABR antibody (AAA19758).Overlay histogram showing 293T cells stained with AAA19758 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABR Antibody (AAA19758, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 5. IF analysis of ABR using anti-ABR antibody (AAA19758).ABR was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABR Antibody (AAA19758) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABR Antibody (AAA19758) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ABR Antibody (AAA19758) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABR antigen affinity purified polyclonal antibody (#AAA19758) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABR at approximately 100 kDa. The expected band size for ABR is at 98 kDa.)

ABR, Polyclonal Antibody (Cat# AAA19758)

Full Name

Anti-ABR Antibody Picoband

Gene Names

ABR; MDB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-UQCRQ AntibodyTitration: 1.0 ug/mlPositive Control: Fetal Liver)

UQCRQ, Polyclonal Antibody (Cat# AAA23598)

Full Name

UQCRQ Antibody - middle region

Gene Names

UQCRQ; QPC; QCR8; QP-C; UQCR7; MC3DN4

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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SAFB, Polyclonal Antibody (Cat# AAA28206)

Full Name

SAFB Polyclonal Antibody

Gene Names

SAFB; HAP; HET; SAFB1; SAF-B1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

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MIB1, Polyclonal Antibody (Cat# AAA30766)

Full Name

MIB1 Rabbit Polyclonal Antibody

Gene Names

MIB1; MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin section), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistochemistry) (GSTP1/GST Pi Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.)

GSTP1/GST Pi, Polyclonal Antibody (Cat# AAA21292)

Full Name

GSTP1/GST Pi Rabbit anti-Human Polyclonal (aa1-210) Antibody

Gene Names

GSTP1; PI; DFN7; GST3; GSTP; FAEES3

Reactivity

Pig, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using [KO Validated] AKT1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

AKT1, Polyclonal Antibody (Cat# AAA22316)

Full Name

(KO Validated) AKT1 Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-RPL36AL AntibodyTitration: 1.0 ug/mlPositive Control: Jurkat Whole CellRPL36AL is supported by BioGPS gene expression data to be expressed in Jurkat)

RPL36AL, Polyclonal Antibody (Cat# AAA23596)

Full Name

RPL36AL Antibody - C-terminal region

Gene Names

RPL36AL; RPL36A

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ANXA8L2 antibody. Blue: DAPI for nuclear staining.)

ANXA8L1, Polyclonal Antibody (Cat# AAA28204)

Full Name

ANXA8L1 Polyclonal Antibody

Gene Names

ANXA8; ANX8; CH17-360D5.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluoresence (IF)

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

BMP-8A, Polyclonal Antibody (Cat# AAA28972)

Full Name

BMP-8A Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Tubulin alpha, Polyclonal Antibody (Cat# AAA29228)

Full Name

Tubulin alpha Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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Transfection Control (Anti-GPR182 / ADMR antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

GPR182 / ADMR, Polyclonal Antibody (Cat# AAA12333)

Full Name

Rabbit Polyclonal to Human GPR182 / ADMR

Gene Names

GPR182; AMR; 7TMR; ADMR; AM-R; G10D; gamrh; hrhAMR

Reactivity

Gorilla, Human, Monkey
Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Bcl-2, Polyclonal Antibody (Cat# AAA22320)

Full Name

(KO Validated) Bcl-2 Polyclonal Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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BMP-2, Polyclonal Antibody (Cat# AAA30762)

Full Name

BMP-2 Rabbit Polyclonal Antibody

Gene Names

BMP2; BDA2; BMP2A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IF (Immunofluorescence) (Figure 12. IF analysis of HCRT using anti-HCRT antibody (AAA19755).HCRT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of HCRT using anti-HCRT antibody (AAA19755).HCRT was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HCRT Antibody (AAA19755) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

HCRT, Polyclonal Antibody (Cat# AAA19755)

Full Name

Anti-HCRT Antibody

Gene Names

HCRT; OX; PPOX; NRCLP1

Reactivity

Mouse, Rat

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using GNE antibody at dilution of 1:100 (40x lens).)

GNE, Polyclonal Antibody (Cat# AAA28213)

Full Name

GNE Polyclonal Antibody

Gene Names

GNE; NM; DMRV; IBM2; Uae1; GLCNE

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Caveolin-1, Polyclonal Antibody (Cat# AAA28981)

Full Name

Caveolin-1 Polyclonal Antibody

Gene Names

CAV1; CGL3; BSCL3; VIP21; MSTP085

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of RT4 cells using anti-NUMA/NUMA1 antibody (AAA19766).Overlay histogram showing RT4 cells stained with AAA19766 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMA/NUMA1 Antibody (AAA19766, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of NUMA/NUMA1 using anti-NUMA/NUMA1 antibody (AAA19766) and anti-Beta Tubulin antibody (M01857-3).NUMA/NUMA1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUMA/NUMA1 Antibody (AAA19766) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUMA/NUMA1 Antibody (AAA19766) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUMA/NUMA1 Antibody (AAA19766) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NUMA/NUMA1 Antibody (AAA19766) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: monkey COS-7 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: human SIHA whole cell lysates,Lane 6: human RT4 whole cell lysates,Lane 7: rat PC-12 whole cell lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMA/NUMA1 antigen affinity purified polyclonal antibody (#AAA19766) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NUMA/NUMA1 at approximately 270 kDa. The expected band size for NUMA/NUMA1 is at 270 kDa.)

NUMA/NUMA1, Polyclonal Antibody (Cat# AAA19766)

Full Name

Anti-NUMA/NUMA1 Antibody Picoband

Gene Names

NUMA1; NUMA

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using SH2D1A antibody at dilution of 1:200 (400x lens).)

SH2D1A/SAP, Polyclonal Antibody (Cat# AAA21302)

Full Name

SH2D1A/SAP Rabbit anti-Human Polyclonal Antibody

Gene Names

SH2D1A; LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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WB (Western Blot) (WB Suggested Anti-FOXM1 Antibody Titration: 1 ug/mlPositive Control: Jurkat cell lysate)

FOXM1, Polyclonal Antibody (Cat# AAA23606)

Full Name

FOXM1 antibody - middle region

Gene Names

FOXM1; MPP2; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1A; FOXM1B; FOXM1C; HFH-11; TRIDENT; MPHOSPH2

Reactivity

Dog, Guinea Pig, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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EPAS-1, Polyclonal Antibody (Cat# AAA30775)

Full Name

EPAS-1 Rabbit Polyclonal Antibody

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

LRRK2, Polyclonal Antibody (Cat# AAA31287)

Full Name

Phospho-LRRK2 (Ser935) Antibody

Gene Names

LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human esophagus Tissue.)

Actin Alpha 2 (ACTa2), Polyclonal Antibody (Cat# AAA21048)

Full Name

Anti-Actin Alpha 2, Smooth Muscle (ACTa2) polyclonal Antibody

Gene Names

ACTA2; ACTSA

Reactivity

Human

Applications

Western Blot (WB)

Purity

Antigen-specific affinity chromatography

Pricing

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IHC (Immunohistochemistry) (PSMA3 Antibody-Immunohistochemistry of paraffin-embedded rat lung using PSMA3 antibody at dilution of 1:200 (400x lens).)

PSMA3, Polyclonal Antibody (Cat# AAA21304)

Full Name

PSMA3 Rabbit anti-Human Polyclonal Antibody

Gene Names

PSMA3; HC8; PSC3

Reactivity

Mouse, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Affinity purified

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WB (Western Blot) (WB Suggested Anti-GABRP AntibodyTitration: 1.25 ug/mlPositive Control: HepG2/Jurkat)

GABRP, Polyclonal Antibody (Cat# AAA23608)

Full Name

GABRP antibody - N-terminal region

Reactivity

Dog, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Protein A purified

Pricing

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METTL14, Polyclonal Antibody (Cat# AAA28216)

Full Name

METTL14 Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IFN-gammaRalpha, Polyclonal Antibody (Cat# AAA29240)

Full Name

IFN-gammaRalpha Polyclonal Antibody

Gene Names

IFNGR1; CD119; IFNGR

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Angptl1, Polyclonal Antibody (Cat# AAA29235)

Full Name

Angptl1 Polyclonal Antibody

Gene Names

ANGPTL1; ANG3; ARP1; AngY; ANGPT3; UNQ162; dJ595C2.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

MAP3K3, Polyclonal Antibody (Cat# AAA31283)

Full Name

Phospho-MAP3K3 (Ser166) Antibody

Gene Names

MAP3K3; MEKK3; MAPKKK3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (GSS/Glutathione Synthetase Antibody-Immunohistochemistry of paraffin-embedded rat kidney using GSS antibody at dilution of 1:200 (x400 lens))

GSS/Glutathione Synthetase, Polyclonal Antibody (Cat# AAA21300)

Full Name

GSS/Glutathione Synthetase Rabbit anti-Human Polyclonal Antibody

Gene Names

GSS; GSHS; HEL-S-64p; HEL-S-88n

Reactivity

Mouse, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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hFANCI, Polyclonal Antibody (Cat# AAA31282)

Full Name

Phospho-hFANCI (Ser565) Antibody

Gene Names

FANCI; KIAA1794

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Transfection Control (Anti-CD97 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

CD97, Polyclonal Antibody (Cat# AAA12339)

Full Name

Rabbit Polyclonal to Human CD97

Gene Names

CD97; TM7LN1

Reactivity

Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of RT4 cells using anti-XDH antibody (AAA19763).Overlay histogram showing RT4 cells stained with AAA19763 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XDH Antibody (AAA19763, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of XDH using anti-XDH antibody (AAA19763).XDH was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-XDH Antibody (AAA19763) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-XDH Antibody (AAA19763) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-XDH Antibody (AAA19763) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-XDH Antibody (AAA19763) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse liver tissue lysates,Lane 3: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XDH antigen affinity purified polyclonal antibody (#AAA19763) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 147-150 kDa.)

XDH, Polyclonal Antibody (Cat# AAA19763)

Full Name

Anti-XDH Antibody Picoband

Gene Names

XDH; XO; XOR

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

Goat F(ab')2 Anti-Human IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA14901)

Full Name

Goat F(ab')2 Anti-Human IgM-FITC

Reactivity

Human

Applications

FLISA, Flow Cytometry (FC).

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-ACAT1 antibody (AAA19765).Overlay histogram showing MCF-7 cells stained with AAA19765 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAT1 Antibody (AAA19765, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of ACAT1 using anti-ACAT1 antibody (AAA19765).ACAT1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ACAT1 Antibody (AAA19765) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ACAT1 Antibody (AAA19765) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-ACAT1 Antibody (AAA19765) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human PC-3 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat skeletal muscle tissue lysates,Lane 7: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAT1 antigen affinity purified polyclonal antibody (#AAA19765) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACAT1 at approximately 40 kDa. The expected band size for ACAT1 is at 40 kDa.)

ACAT1, Polyclonal Antibody (Cat# AAA19765)

Full Name

Anti-ACAT1 Antibody Picoband

Gene Names

ACAT1; T2; MAT; ACAT; THIL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (PPP1CB Antibody-Immunohistochemistry of paraffin-embedded human lung cancer using PPP1CB antibody at dilution of 1:200 (200x lens).)

PPP1CB, Polyclonal Antibody (Cat# AAA21301)

Full Name

PPP1CB Rabbit anti-Human Polyclonal Antibody

Gene Names

PPP1CB; PP1B; PP-1B; PPP1CD; PP1beta; HEL-S-80p

Reactivity

Mouse, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

CD148, Polyclonal Antibody (Cat# AAA29242)

Full Name

CD148 Polyclonal Antibody

Gene Names

PTPRJ; DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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Viewing 1250-1300 of 3252 product results

JTB, Polyclonal Antibody (Cat# AAA17962)

Heme Oxygenase 1, Polyclonal Antibody (Cat# AAA22319)

FOXN2, Polyclonal Antibody (Cat# AAA28719)

Caspase-1, Polyclonal Antibody (Cat# AAA28975)

Calsenilin/KCNIP3, Polyclonal Antibody (Cat# AAA31279)

DIS3, Polyclonal Antibody (Cat# AAA19760)

GPM6A, Polyclonal Antibody (Cat# AAA23595)

p73, Polyclonal Antibody (Cat# AAA29227)

HDGF, Polyclonal Antibody (Cat# AAA30763)

Lat, Polyclonal Antibody (Cat# AAA19756)

BMP5, Polyclonal Antibody (Cat# AAA28209)

CRFR1 / CRHR1, Polyclonal Antibody (Cat# AAA12338)

RAD51C, Polyclonal Antibody (Cat# AAA19762)

MDC1, Polyclonal Antibody (Cat# AAA28205)

ABR, Polyclonal Antibody (Cat# AAA19758)

UQCRQ, Polyclonal Antibody (Cat# AAA23598)

SAFB, Polyclonal Antibody (Cat# AAA28206)

MIB1, Polyclonal Antibody (Cat# AAA30766)

GSTP1/GST Pi, Polyclonal Antibody (Cat# AAA21292)

AKT1, Polyclonal Antibody (Cat# AAA22316)

RPL36AL, Polyclonal Antibody (Cat# AAA23596)

ANXA8L1, Polyclonal Antibody (Cat# AAA28204)

BMP-8A, Polyclonal Antibody (Cat# AAA28972)

Tubulin alpha, Polyclonal Antibody (Cat# AAA29228)

GPR182 / ADMR, Polyclonal Antibody (Cat# AAA12333)

Bcl-2, Polyclonal Antibody (Cat# AAA22320)

BMP-2, Polyclonal Antibody (Cat# AAA30762)

HCRT, Polyclonal Antibody (Cat# AAA19755)

GNE, Polyclonal Antibody (Cat# AAA28213)

Caveolin-1, Polyclonal Antibody (Cat# AAA28981)

NUMA/NUMA1, Polyclonal Antibody (Cat# AAA19766)

SH2D1A/SAP, Polyclonal Antibody (Cat# AAA21302)

FOXM1, Polyclonal Antibody (Cat# AAA23606)

EPAS-1, Polyclonal Antibody (Cat# AAA30775)

LRRK2, Polyclonal Antibody (Cat# AAA31287)

Actin Alpha 2 (ACTa2), Polyclonal Antibody (Cat# AAA21048)

PSMA3, Polyclonal Antibody (Cat# AAA21304)

GABRP, Polyclonal Antibody (Cat# AAA23608)

METTL14, Polyclonal Antibody (Cat# AAA28216)

IFN-gammaRalpha, Polyclonal Antibody (Cat# AAA29240)

Angptl1, Polyclonal Antibody (Cat# AAA29235)

MAP3K3, Polyclonal Antibody (Cat# AAA31283)

GSS/Glutathione Synthetase, Polyclonal Antibody (Cat# AAA21300)

hFANCI, Polyclonal Antibody (Cat# AAA31282)

CD97, Polyclonal Antibody (Cat# AAA12339)

XDH, Polyclonal Antibody (Cat# AAA19763)

Goat F(ab')2 Anti-Human IgM (u chain specific), Polyclonal Secondary Antibody (Cat# AAA14901)

ACAT1, Polyclonal Antibody (Cat# AAA19765)

PPP1CB, Polyclonal Antibody (Cat# AAA21301)

CD148, Polyclonal Antibody (Cat# AAA29242)

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