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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1100-1150 of 3176 product results
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DGCR8 antibody . Western blot was performed from the immunoprecipitate using DGCR8 antibody at a dilition of 1:1000.)
DGCR8, Polyclonal Antibody (Cat# AAA28373)
Full Name
DGCR8 Rabbit pAb
Gene Names
DGCR8; Gy1; pasha; DGCRK6; C22orf12
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using LLGL1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
LLGL1, Polyclonal Antibody (Cat# AAA28385)
Full Name
LLGL1 Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
IL-4, Polyclonal Antibody (Cat# AAA29156)
Full Name
IL-4 Polyclonal Antibody
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Reactivity
Human
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF7 cell using PPP4C antibody. Blue: DAPI for nuclear staining.)
PPP4C, Polyclonal Antibody (Cat# AAA29677)
Full Name
PPP4C Antibody
Gene Names
PPP4C; PP4; PPX; PP-X; PP4C; PPH3; PPP4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
Application Data
(Dilution: WB 1:1000-2000, IHC 1:100-200)
Kv11.3, Polyclonal Antibody (Cat# AAA28906)
Full Name
Kv11.3 Polyclonal Antibody
Gene Names
KCNH7; ERG3; HERG3; Kv11.3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
WB (Western Blot)
(WB Suggested Anti-UNC84A Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysate)
UNC84A, Polyclonal Antibody (Cat# AAA23537)
Full Name
UNC84A antibody - N-terminal region
Gene Names
SUN1; UNC84A
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using LLGL1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
LLGL1, Polyclonal Antibody (Cat# AAA22271)
Full Name
LLGL1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Lung cancer Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human MASP1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Mannose Associated Serine Protease 1, Polyclonal Antibody (Cat# AAA20912)
Full Name
Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1)
Gene Names
MASP1; 3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of HeLa cells using anti-RPL23 antibody (AAA19565).Overlay histogram showing HeLa cells stained with AAA19565 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL23 Antibody (AAA19565, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RPL23, Polyclonal Antibody (Cat# AAA19565)
Full Name
Anti-RPL23 Antibody Picoband
Gene Names
RPL23; L23; rpL17
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U87 cells using anti-PDE6 beta/PDE6B antibody (AAA19261).Overlay histogram showing U87 cells stained with AAA19261 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE6 beta/PDE6B Antibody (AAA19261,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDE6 beta/PDE6B, Polyclonal Antibody (Cat# AAA19261)
Full Name
Anti-PDE6 beta/PDE6B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)
EOMES, Polyclonal Antibody (Cat# AAA26889)
Full Name
EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)
Gene Names
Eomes; Tbr2; TBR-2; C77258
Reactivity
Mouse, Human, Rat
Applications
IHC, WB
Purity
Purified by affinity chromatography.
Pricing
IF (Immunofluorescence)
(IF staining GRP78 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).)
GRP78, Polyclonal Antibody (Cat# AAA29964)
Full Name
GRP78 Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
WB (Western Blot)
(Western Blot; Samples: Lane1: Jurkat cell lysate; Lane2: Raji cell lysate; Lane3: NIH/3T3 cell lysate; Lane4: RAW264.7 cell lysate;Primary Ab: 0.1ug/ml Rabbit Anti-Human SSB AntibodySecond Ab: 0.2ug/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Sjogren Syndrome Antigen B (SSB), Polyclonal Antibody (Cat# AAA21267)
Full Name
Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB)
Gene Names
SSB; La; LARP3
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD241, Polyclonal Antibody (Cat# AAA29204)
Full Name
CD241 Polyclonal Antibody
Gene Names
RHAG; RH2; Rh50; CD241; RH50A; Rh50GP; SLC42A1
Reactivity
Human
Applications
WB, IHC-P
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.)
Rad21, Polyclonal Antibody (Cat# AAA22310)
Full Name
Rad21 Polyclonal Antibody
Gene Names
RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(AF4499 at 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PERK, Polyclonal Antibody (Cat# AAA31277)
Full Name
Phospho-PERK (Ser1096) Antibody
Reactivity
Human, mouse, rat
Applications
WB, IHC, EIA
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using DDX3Y antibody at 1:3000 dilution.)
DDX3Y, Polyclonal Antibody (Cat# AAA30552)
Full Name
DDX3Y Polyclonal Antibody
Gene Names
DDX3Y; DBY
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2A, Polyclonal Antibody (Cat# AAA31347)
Full Name
Acetyl-Histone H2A (Lys5) Antibody
Gene Names
HIST1H2AB; H2A/m; H2AFM
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CHK2, Polyclonal Antibody (Cat# AAA31398)
Full Name
Phospho-CHK2 (Ser33+Ser35) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (82%)
Predicted Reactivity: Bovine (82%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110β (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110β (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA14720)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CB; PI3K; PIK3C1; P110BETA; PI3KBETA; MGC133043; DKFZp779K1237
Reactivity
Human
Applications
EL/EIA, WB, IP, IHC, ICC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
CD19, Polyclonal Antibody (Cat# AAA29305)
Full Name
CD19 Polyclonal Antibody
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody.)
Histone H2B-K12, Polyclonal Antibody (Cat# AAA29816)
Full Name
Acetyl-Histone H2B-K12 pAb
Gene Names
HIST2H2BE; H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using BRCA1 antibody.)
BRCA1, Polyclonal Antibody (Cat# AAA10639)
Full Name
BRCA1 Polyclonal Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, ELISA 1:10000-20000)
CLECSF6, Polyclonal Antibody (Cat# AAA29339)
Full Name
CLECSF6 Polyclonal Antibody
Gene Names
CLEC4A; DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
Reactivity
Human
Applications
WB
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (AAA19147).VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 3 Antibody (AAA19147) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
VEGF Receptor 3, Polyclonal Antibody (Cat# AAA19147)
Full Name
Anti-VEGF Receptor 3 Picoband antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB, FC
Purity
Immunoggen affinity purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Fractalkine Receptor, Polyclonal Antibody (Cat# AAA29147)
Full Name
Fractalkine Receptor Polyclonal Antibody
Gene Names
CX3CR1; V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of C6 cells using AIF Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
AIF, Polyclonal Antibody (Cat# AAA22250)
Full Name
AIF Polyclonal Antibody
Gene Names
AIFM1; AIF; CMTX4; COWCK; PDCD8; COXPD6
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of TTC11/FIS1 using anti-TTC11/FIS1 antibody (AAA19439).TTC11/FIS1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TTC11/FIS1 Antibody (AAA19439) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
TTC11/FIS1, Polyclonal Antibody (Cat# AAA19439)
Full Name
Anti-TTC11/FIS1 Antibody Picoband
Gene Names
FIS1; TTC11; CGI-135
Reactivity
Human, Rat
Applications
WB, IHC, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HeLa cells using anti-SLU7 antibody (AAA19583).Overlay histogram showing HeLa cells stained with AAA19583 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLU7 Antibody (AAA19583, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SLU7, Polyclonal Antibody (Cat# AAA19583)
Full Name
Anti-SLU7 Antibody Picoband
Gene Names
SLU7; 9G8; hSlu7
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
ICC (Immunocytochemistry)
(ICC staining MSH6 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
MSH6, Polyclonal Antibody (Cat# AAA29950)
Full Name
MSH6 Antibody
Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of K-562 cells usingug hnRNP C Polyclonal Antibody. Western blot was performed from the immunoprecipitate using hnRNP C Polyclonal Antibody at a dilution of 1:1000.)
hnRNP C, Polyclonal Antibody (Cat# AAA22269)
Full Name
hnRNP C Polyclonal Antibody
Gene Names
HNRNPC; C1; C2; HNRNP; HNRPC; SNRPC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
14-3-3 sigma, Polyclonal Antibody (Cat# AAA28959)
Full Name
14-3-3 sigma Polyclonal Antibody
Gene Names
SFN; YWHAS
Reactivity
Human, Mouse
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD2, Polyclonal Antibody (Cat# AAA29190)
Full Name
CD2 Polyclonal Antibody
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U2OS cells using anti-RBP1 antibody (AAA19800).Overlay histogram showing U2OS cells stained with AAA19800 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBP1 Antibody (AAA19800, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RBP1, Polyclonal Antibody (Cat# AAA19800)
Full Name
Anti-RBP1 Antibody Picoband
Gene Names
RBP1; CRBP; RBPC; CRBP1; CRBPI; CRABP-I
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-SSBP2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:7812500Positive Control: Raji cell lysate)
SSBP2, Polyclonal Antibody (Cat# AAA23390)
Full Name
SSBP2 antibody - N-terminal region
Gene Names
SSBP2; HSPC116; SOSS-B2
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
GLI1, Polyclonal Antibody (Cat# AAA30569)
Full Name
GLI1 Polyclonal Antibody
Gene Names
GLI1; GLI
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: U251 (Human) Cell Lysate at 30 ug Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78/74 kD Observed band size: 78/74 kD)
MARCH7, Polyclonal Antibody (Cat# AAA28826)
Full Name
MARCH7 Polyclonal Antibody
Gene Names
MARCH7; AXO; AXOT; RNF177; MARCH-VII
Reactivity
Rat, Pig, Dog, Cow
Applications
WB, IHC-P, IHC-F, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using HNRNPD Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
HNRNPD, Polyclonal Antibody (Cat# AAA22189)
Full Name
HNRNPD Polyclonal Antibody
Gene Names
HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0
Reactivity
Human, Mouse, Rat
Applications
IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-JunD (Ser259) in lysates of K562 , using Phospho-JunD (Ser259) Antibody.)
JunD, Polyclonal Antibody (Cat# AAA31152)
Full Name
Phospho-JunD (Ser259) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Dog, Chicken
Predicted: Pig, Zebrafish, Bovine, Dog, Chicken
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using DNAJB6 antibody.)
DNAJB6, Polyclonal Antibody (Cat# AAA29837)
Full Name
DNAJB6 Polyclonal Antibody
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using BRMS1 at dilution of 1:100 (40x lens).)
BRMS1, Polyclonal Antibody (Cat# AAA30626)
Full Name
BRMS1 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using SMAD9 antibody. Blue: DAPI for nuclear staining.)
SMAD9, Polyclonal Antibody (Cat# AAA28113)
Full Name
SMAD9
Gene Names
SMAD9; PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
IF (Immunofluorescence)
(Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 μg/mL.)
MDA5, Polyclonal Antibody (Cat# AAA10956)
Full Name
MDA5 Antibody
Gene Names
IFIH1; AGS7; Hlcd; MDA5; MDA-5; RLR-2; IDDM19
Reactivity
Human, Mouse
Applications
EIA, WB, IHC-P, IF
Purity
MDA5 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
LXRalpha, Polyclonal Antibody (Cat# AAA29155)
Full Name
LXRalpha Polyclonal Antibody
Gene Names
NR1H3; LXRA; LXR-a; RLD-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using DNAJB6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
DNAJB6, Polyclonal Antibody (Cat# AAA28129)
Full Name
DNAJB6 Polyclonal Antibody
Gene Names
DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-CHST2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
CHST2, Polyclonal Antibody (Cat# AAA23524)
Full Name
CHST2 antibody - middle region
Gene Names
CHST2; C6ST; GST2; GST-2; Gn6ST-1; HEL-S-75; glcNAc6ST-1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26862)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 550)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Fibulin 5, Polyclonal Antibody (Cat# AAA29940)
Full Name
Fibulin 5 Antibody
Gene Names
FBLN5; EVEC; UP50; ADCL2; ARMD3; DANCE; ARCL1A; FIBL-5
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Protein affinity purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of THP-1 cells using anti-YTHDF2 antibody (AAA19260).Overlay histogram showing THP-1 cells stained with AAA19260 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YTHDF2 Antibody (AAA19260, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
YTHDF2, Polyclonal Antibody (Cat# AAA19260)
Full Name
Anti-YTHDF2 Antibody
Gene Names
YTHDF2; HGRG8; NY-REN-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9/Rad9a antibody (AAA19288).Overlay histogram showing HEPA1-6 cells stained with AAA19288 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9/Rad9a Antibody (AAA19288,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rad9/Rad9a, Polyclonal Antibody (Cat# AAA19288)
Full Name
Anti-Rad9/Rad9a Antibody
Gene Names
Rad9a; Rad9
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.