Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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ApoA-I, Polyclonal Antibody (Cat# AAA29256)

Full Name

ApoA-I Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Figure 6. IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody (AAA19785).Islet 1/ISL1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-Islet 1/ISL1 Antibody (AAA19785) overnight at 4 degree C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 5. IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody (AAA19785).Islet 1/ISL1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-Islet 1/ISL1 Antibody (AAA19785) overnight at 4 degree C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 4. IF analysis of Islet 1/ISL1 using anti-Islet 1/ISL1 antibody (AAA19785) and anti-Beta Tubulin antibody (M01857-3).Islet 1/ISL1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Islet 1/ISL1 Antibody (AAA19785) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Islet 1/ISL1 Antibody (AAA19785) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Islet 1/ISL1 antigen affinity purified polyclonal antibody (#AAA19785) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (45 kDa.)

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Islet 1/ISL1, Polyclonal Antibody (Cat# AAA19785)

Full Name

Anti-Islet 1/ISL1 Antibody Picoband

Gene Names

ISL1; Isl-1; ISLET1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry)

WB (Western Blot)

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ZNF595, Polyclonal Antibody (Cat# AAA17999)

Full Name

ZNF595 Antibody

Reactivity

Reacts with: Human
Predicted: Rat, Mouse.
Not yet tested in other species.

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Pricing

$435/0.1 mg $695/0.1 mL (AF350) $695/0.1 mL (AF405L) $695/0.1 mL (AF405M) $695/0.1 mL (AF405S) $695/0.1 mL (AF488) $695/0.1 mL (AF546) $695/0.1 mL (AF555) $695/0.1 mL (AF568) $695/0.1 mL (AF594) $695/0.1 mL (AF610) $695/0.1 mL (AF635) $695/0.1 mL (AF647) $695/0.1 mL (AF680) $695/0.1 mL (AF750) $695/0.1 mL (AP) $695/0.1 mL (APC) $695/0.1 mL (Biotin) $695/0.1 mL (Cy3) $695/0.1 mL (Cy5) $695/0.1 mL (Cy5.5) $695/0.1 mL (Cy7) $695/0.1 mL (FITC) $695/0.1 mL (HRP) $695/0.1 mL (PE) $1,345/2x0.1 mL (AF350) $1,955/3x0.1 mL (AF350) $2,535/4x0.1 mL (AF350)

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IHC (Immunohistochemistry) (PCNA Antibody-Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:200.)

IHC (Immunohistchemistry) (PCNA Antibody-Immunohistochemistry of paraffin-embedded human gastric tissue, at a dilution of 1:200.)

IHC (Immunohistochemistry) (PCNA Antibody-Immunohistochemistry of paraffin-embedded mouse lung tissue, at a dilution of 1:200.)

IHC (Immunohistochemistry) (PCNA Antibody-Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)

IHC (Immunohistochemistry) (PCNA Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)

WB (Western Blot) (PCNA Antibody-Western blot analysis of extracts of various cell lines, using PCNA antibody.)

WB (Western Blot) (PCNA Antibody-Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.)

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PCNA, Polyclonal Antibody (Cat# AAA21327)

Full Name

PCNA Rabbit anti-Human Polyclonal Antibody

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

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Cyclin D1, Polyclonal Antibody (Cat# AAA29007)

Full Name

Cyclin D1 Polyclonal Antibody

Gene Names

CCND1; BCL1; PRAD1; U21B31; D11S287E

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MMP16 antibody at 1:1000 dilution.)

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MMP16, Polyclonal Antibody (Cat# AAA30543)

Full Name

MMP16 Polyclonal Antibody

Gene Names

MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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Transfection Control (Anti-GPR45 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Transfection (Anti-GPR45 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR45.)

IHC (Immunohistochemistry) (Anti-GPR45 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR45 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR45 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR45 antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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GPR45, Polyclonal Antibody (Cat# AAA12368)

Full Name

Rabbit Polyclonal to Human GPR45

Gene Names

GPR45; PSP24; PSP24A; PSP24(ALPHA)

Reactivity

Gibbon, Gorilla, Horse, Human, Monkey
Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

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IHC (Immunohistchemistry) (Anti-CXCR2 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CXCR2 antibody IHC of human synovium, rheumatoid arthritis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CXCR2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CXCR2 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CXCR2 antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-CXCR2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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CXCR2, Polyclonal Antibody (Cat# AAA12364)

Full Name

Rabbit Polyclonal to Human CXCR2

Gene Names

CXCR2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b

Reactivity

Chimpanzee, Human, Monkey

Applications

Immunohistochemistry (IHC - Paraffin)

Purity

Immunoaffinity Purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-PRDM2 antibody (AAA19788).Overlay histogram showing HepG2 cells stained with AAA19788 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDM2 Antibody (AAA19788, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PRDM2 using anti-PRDM2 antibody (AAA19788) and anti-Beta Tubulin antibody (M01857-3).PRDM2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM2 Antibody (AAA19788) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM2 Antibody (AAA19788) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM2 Antibody (AAA19788) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PRDM2 Antibody (AAA19788) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM2 antigen affinity purified polyclonal antibody (#AAA19788) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (250-280 kDa.)

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PRDM2, Polyclonal Antibody (Cat# AAA19788)

Full Name

Anti-PRDM2 Antibody Picoband

Gene Names

PRDM2; RIZ; KMT8; RIZ1; RIZ2; KMT8A; MTB-ZF; HUMHOXY1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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Moesin/Ezrin/Radixin, Polyclonal Antibody (Cat# AAA31305)

Full Name

Phospho-Moesin/Ezrin/Radixin (Thr558) Antibody

Gene Names

EZR; CVL; CVIL; VIL2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (KRT19/CK19/Cytokeratin 19 Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue, with a concentration of 1:100.)

IHC (Immunohistochemistry) (KRT19/CK19/Cytokeratin 19 Antibody-Immunohistochemistry of paraffin-embedded rat kidney tissue, with a concentration of 1:100.)

IHC (Immunohistochemistry) (KRT19/CK19/Cytokeratin 19 Antibody-Immunohistochemistry of paraffin-embedded mouse heart, with a concentration of 1:100.)

IHC (Immunohistochemistry) (KRT19/CK19/Cytokeratin 19 Antibody-Immunohistochemistry of paraffin-embedded mouse kidney tissue, with a concentration of 1:100.)

IHC (Immunohistochemistry) (KRT19/CK19/Cytokeratin 19 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:200.)

WB (Western Blot) (KRT19/CK19/Cytokeratin 19 Antibody-Western blot analysis of extracts of mouse stomach, using KRT19 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 120s.)

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KRT19/CK19/Cytokeratin 19, Polyclonal Antibody (Cat# AAA21322)

Full Name

KRT19/CK19/Cytokeratin 19 Rabbit anti-Human Polyclonal Antibody

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

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IHC (Immunohistchemistry) (Immunochemical staining MSH6 in Ferret spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MSH6 in rat spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MSH6 in mouse spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MSH6 in cynomolgus spleen with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MSH6 in human colon carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining MSH6 in human rectal cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

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MSH6, Polyclonal Antibody (Cat# AAA27722)

Full Name

Anti-MSH6 Antibody, Rabbit Polyclonal

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human, Cynomolgus, Mouse, Rat, Ferret

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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MAPK Organizer 1, Polyclonal Antibody (Cat# AAA29258)

Full Name

MAPK Organizer 1 Polyclonal Antibody

Gene Names

WDR83; MORG1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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Transfection Control (Anti-P2RY6 / P2Y6 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Transfection (Anti-P2RY6 / P2Y6 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with P2RY6 / P2Y6.)

IHC (Immunohistochemistry) (Anti-P2RY6 / P2Y6 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-P2RY6 / P2Y6 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-P2RY6 / P2Y6 antibody IHC of human spleen, red pulp. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-P2RY6 / P2Y6 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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P2RY6 / P2Y6, Polyclonal Antibody (Cat# AAA12363)

Full Name

Rabbit Polyclonal to Human P2RY6 / P2Y6

Gene Names

P2RY6; P2Y6

Reactivity

Gibbon, Gorilla, Human, Monkey, Orangutan
Predicted Reactivity: Dog, Pig (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

$630/0.05 mg $1,215/2x0.05 mg $1,770/3x0.05 mg $2,295/4x0.05 mg $2,575/5x0.05 mg

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-GBP1 antibody (AAA19787).Overlay histogram showing MCF-7 cells stained with AAA19787 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GBP1 Antibody (AAA19787, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IP (Immunoprecipitation) (Figure 6. Immunoprecipitating GBP1 in Hela whole cell lysate .Western blot analysis of GBP1 using anti-GBP1 antibody (AAA19787).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-GBP1 antibody in Hela whole cell lysate.Lane 3: anti-GBP1 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GBP1 antigen affinity purified polyclonal antibody (AAA19787) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GBP1 Antibody (AAA19787) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GBP1 Antibody (AAA19787) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GBP1 Antibody (AAA19787) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HUVEC whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat spleen tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse spleen tissue lysayes.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBP1 antigen affinity purified polyclonal antibody (#AAA19787) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GBP1 at approximately 68 kDa. The expected band size for GBP1 is at 68 kDa.)

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GBP1, Polyclonal Antibody (Cat# AAA19787)

Full Name

Anti-GBP1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

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c-Src, Polyclonal Antibody (Cat# AAA29003)

Full Name

c-Src Polyclonal Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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FOXP3, Polyclonal Antibody (Cat# AAA29259)

Full Name

FOXP3 Polyclonal Antibody

Gene Names

FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of Raji cells using anti-PSMD4 antibody (AAA19792).Overlay histogram showing Raji cells stained with AAA19792 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMD4 Antibody (AAA19792, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of PSMD4 using anti-PSMD4 antibody (AAA19792).PSMD4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 9. IF analysis of PSMD4 using anti-PSMD4 antibody (AAA19792).PSMD4 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 8. IF analysis of PSMD4 using anti-PSMD4 antibody (AAA19792) and anti-Beta Tubulin antibody (M01857-3).PSMD4 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PSMD4 Antibody (AAA19792) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human Raji whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMD4 antigen affinity purified polyclonal antibody (#AAA19792) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSMD4 at approximately 55 kDa. The expected band size for PSMD4 is at 41 kDa.)

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PSMD4, Polyclonal Antibody (Cat# AAA19792)

Full Name

Anti-PSMD4 Antibody Picoband

Gene Names

PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (Western blot analysis of extracts of A-431 cells, using CREB1 antibody at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CREB1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CREB1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CREB1 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using CREB1 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

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CREB1, Polyclonal Antibody (Cat# AAA30545)

Full Name

CREB1 Polyclonal Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31057 at 1/200 staining human colon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31057 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Acetyl-Histone H3 phosphorylation expression in TSA treated RAW264.7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Acetyl-Histone H3 phosphorylation expression in mouse muscle and mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

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Acetyl-Histone H3, Polyclonal Antibody (Cat# AAA31057)

Full Name

Acetyl-Histone H3 (Lys9) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

Immunogen affinity purified

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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WB (Western Blot) (CYK18 Antibody (C-term) Western blot of A431 cell line lysates (35 ug/lane). This demonstrates the CYK18 antibody detected the CYK18 protein (arrow) (Kindly offered by Dr. Li).)

WB (Western Blot) (Western blot of CYK18 Antibody (C-term) in K562, NCI-H460 cell line lysates and mouse stomach tissues lysates(35 ug/lane). CYK18(arrow) was detected using the purified Pab.)

FCM (Flow Cytometry) (Flow cytometry of WiDr cells using CYK18 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

FCM (Flow Cytometry) (Formalin-fixed and paraffin-embedded human colon carcinoma with CYK18 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

IF (Immunofluorescence) (Confocal immunofluorescence of CYK18 Antibody (C-term) with prostate carcinoma followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of CYK18 Antibody (C-term) with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemistry) (Anti-KRT18 / CK18 / Cytokeratin 18 antibody IHC staining of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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KRT18 / CK18 / Cytokeratin 18, Polyclonal Antibody (Cat# AAA12370)

Full Name

Rabbit Polyclonal to Human KRT18 / CK18 / Cytokeratin 18

Gene Names

KRT18; K18; CYK18

Reactivity

Human, Mouse

Applications

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Ammonium sulfate precipitation

Pricing

$650/0.2 mL $1,255/2x0.2 mL $1,825/3x0.2 mL $2,365/4x0.2 mL $2,680/5x0.2 mL

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IHC (Immunohistochemistry) (PSMB8/LMP7 Antibody-Immunohistochemistry of paraffin-embedded rat heart, at a dilution of 1:100.)

IHC (Immunohistchemistry) (PSMB8/LMP7 Antibody-Immunohistochemistry of paraffin-embedded human colon tissue, at a dilution of 1:100.)

IHC (Immunohistochemistry) (PSMB8/LMP7 Antibody-Immunohistochemistry of paraffin-embedded human kidney tissue, at a dilution of 1:100.)

IHC (Immunohistochemistry) (PSMB8/LMP7 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (PSMB8/LMP7 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE), at a dilution of 1:100.)

WB (Western Blot) (PSMB8/LMP7 Antibody-Western blot analysis of extracts of various cell lines.)

ICC (Immunocytochemistry) (PSMB8/LMP7 Antibody-Immunofluorescence analysis of A549 cells.)

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PSMB8/LMP7, Polyclonal Antibody (Cat# AAA21331)

Full Name

PSMB8/LMP7 Rabbit anti-Human Polyclonal Antibody

Gene Names

PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PSMB5i; RING10; D6S216E

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

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DGAT2L3, Polyclonal Antibody (Cat# AAA29011)

Full Name

DGAT2L3 Polyclonal Antibody

Gene Names

AWAT1; DGA2; DGAT2L3

Reactivity

Human

Applications

Western Blot (WB)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

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Cytokeratin 19, Polyclonal Antibody (Cat# AAA29010)

Full Name

Cytokeratin 19 Polyclonal Antibody

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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IL-1RII, Polyclonal Antibody (Cat# AAA29266)

Full Name

IL-1RII Polyclonal Antibody

Gene Names

IL1R2; IL1RB; CD121b; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using FAK antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PTK2 antibody at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using FAK antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of RAW264.7 cells using FAK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC12 cells using FAK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using FAK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

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FAK, Polyclonal Antibody (Cat# AAA30546)

Full Name

FAK Polyclonal Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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CMTM5, Polyclonal Antibody (Cat# AAA29271)

Full Name

CMTM5 Polyclonal Antibody

Gene Names

CMTM5; CKLFSF5

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Rat heart, using Phospho-alpha 1 Catenin (Ser655/Thr658) Antibody. The lane on the left was treated with blocking peptide.)

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alpha 1 Catenin, Polyclonal Antibody (Cat# AAA31319)

Full Name

Phospho-alpha 1 Catenin (Ser655/Thr658) Antibody

Gene Names

CTNNA1; CAP102

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-HHEX antibody Titration: 1 ug/mLSample Type: Human HepG2)

WB (Western Blot) (WB Suggested Anti-HHEX antibody Titration: 1 ug/mLSample Type: Human heart)

WB (Western Blot) (WB Suggested Anti-HHEX Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: K562 cell lysateHHEX is strongly supported by BioGPS gene expression data to be expressed in Human K562 cells)

WB (Western Blot) (Host: RabbitTarget Name: HHEXSample Tissue: Human 293T Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-HHEX AntibodyParaffin Embedded Tissue: Human alveolar cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HHEX AntibodyParaffin Embedded Tissue: Human alveolar cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HHEX AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult heartObserved Staining: Nuclei in adipocytes but not in cardiomyocytesPrimary Antibody Concentration: 1:100Secondary Antibody: Donkey anti-Rabbit-Cy2/3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 secProtocol located in Reviews and Data.)

IHC (Immunohistochemistry) (HumanMuscle)

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HHEX, Polyclonal Antibody (Cat# AAA23384)

Full Name

HHEX antibody - middle region

Gene Names

HHEX; HEX; PRH; HMPH; PRHX; HOX11L-PEN

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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Histone H3, Polyclonal Antibody (Cat# AAA31322)

Full Name

Phospho-Histone H3 (Thr3) Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (GNAQ Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (GNAQ Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (GNAQ Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Eye, Melanoma: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Eye, Melanoma: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Brain, Pilocitic Astrocytoma: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (GNAQ Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

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GNAQ, Polyclonal Antibody (Cat# AAA21339)

Full Name

GNAQ Rabbit anti-Human Polyclonal Antibody

Gene Names

GNAQ; GAQ; SWS; CMC1; G-ALPHA-q

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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CRF-RI, Polyclonal Antibody (Cat# AAA29273)

Full Name

CRF-RI Polyclonal Antibody

Gene Names

CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRHR1f; CRF-R-1; CRH-R-1; CRH-R1h

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-NSUN2 antibody (AAA19802).Overlay histogram showing 293T cells stained with AAA19802 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSUN2 Antibody (AAA19802, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of NSUN2 using anti-NSUN2 antibody (AAA19802).NSUN2 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NSUN2 Antibody (AAA19802) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN2 antigen affinity purified polyclonal antibody (#AAA19802) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NSUN2 at approximately 100 kDa. The expected band size for NSUN2 is at 100 kDa.)

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NSUN2, Polyclonal Antibody (Cat# AAA19802)

Full Name

Anti-NSUN2 Antibody Picoband

Gene Names

NSUN2; MISU; MRT5; SAKI; TRM4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-ERCC8 antibody Titration: 1 ug/mLSample Type: Human liver)

WB (Western Blot) (WB Suggested Anti-ERCC8 Antibody Titration: 0.1-5.0ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: MCF7 Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human HepG2Antibody Dilution: 1.0ug/mlERCC8 is supported by BioGPS gene expression data to be expressed in HepG2)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (HumanMuscle)

IHC (Immunohistochemistry) (Human Liver)

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ERCC8, Polyclonal Antibody (Cat# AAA23386)

Full Name

ERCC8 antibody - C-terminal region

Gene Names

ERCC8; CSA; CKN1; UVSS2

Reactivity

Dog, Horse, Human, Pig, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human testis tissue using AAA26968 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using AAA26968 at dilution of 1:100)

IP (Immunoprecipitation) (Immunoprecipitating STAT5B in K562 whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26968 in K562 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26968 (8ug)+ K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (10ug))

IHC (Immunohistochemistry) (Immunofluorescent analysis of HepG2 cells using AAA26968 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human spleen tissue using AAA26968 at dilution of 1:100)

WB (Western Blot) (Western BlotPositive WB detected in: Jurkat whole cell lysate,K562 whole cell lysate,Rat liver tissue,Mouse liver tiaaue,Mouse lung tissue,Mouse kidney tissueAll lanes: STAT5B antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 90 KDaObserved band size: 90 KDa)

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STAT5B, Polyclonal Antibody (Cat# AAA26968)

Full Name

STAT5B Antibody

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

>95%, Protein G purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

WB (Western Blot) ((0.03ug/ml) staining of HeLa (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminesce)

WB (Western Blot) ((0.1ug/ml) staining of Mouse Liver (A) and (0.03ug/ml) Rat Heart (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence)

WB (Western Blot) ((0.1ug/ml) staining of Human Liver (A), (0.03ug/ml) Testes (B) and Tonsil (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence)

IHC (Immunohistochemistry) ((20ug/ml) staining of PFA-perfused cryosection of Rat Liver. Antigen retrieval with citrate buffer pH 3 by microwave for 20min, Cy3 detection. Data obtained by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.)

IHC (Immunohistochemistry) ((2ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

WB (Western Blot) ((0.5ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was overnight. Detected by alkaline phosphatase (NBT/BCIP). Data provided by Dr. I. Sabolic, Institute for Medical Research and Occupational Health, Zagreb, Croatia.)

WB (Western Blot) ((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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GAPDH, Polyclonal Antibody (Cat# AAA13657)

Full Name

Goat anti-GAPDH (Internal) Antibody

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog

Applications

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$455/0.1 mg $875/2x0.1 mg $1,270/3x0.1 mg $1,645/4x0.1 mg $1,815/5x0.1 mg

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IHC (Immunohistchemistry) (Immunochemical staining CLTC in rat kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining CLTC in mouse kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining CLTC in human adrenal gland with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining CLTC in human colon with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining CLTC in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-CLTC rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:191 kDa)

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CLTC, Polyclonal Antibody (Cat# AAA27734)

Full Name

Anti-CLTC Antibody, Rabbit Polyclonal

Gene Names

CLTC; Hc; CHC; CHC17; CLH-17; CLTCL2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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Dnmt3b, Polyclonal Antibody (Cat# AAA29013)

Full Name

Dnmt3b Polyclonal Antibody

Gene Names

DNMT3B; ICF; ICF1; M.HsaIIIB

Reactivity

Human, Chicken

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (TMPO/TP/Thymopoietin Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (TMPO/TP/Thymopoietin Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (TMPO/TP/Thymopoietin Antibody-Western Blot; Sample: Mouse Thymus lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TMPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (TMPO/TP/Thymopoietin Antibody-Western blot of TMPO/TP/Thymopoietin antibody.)

WB (Western Blot) (TMPO/TP/Thymopoietin Antibody-Western Blot; Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TMPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (TMPO/TP/Thymopoietin Antibody-Western Blot; Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human TMPO Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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TMPO/TP/Thymopoietin, Polyclonal Antibody (Cat# AAA21334)

Full Name

TMPO/TP/Thymopoietin Rabbit anti-Human Polyclonal (aa1-243) Antibody

Gene Names

TMPO; TP; LAP2; CMD1T; LEMD4; PRO0868

Reactivity

Mouse, Rat, Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using mTOR antibody at dilution of 1:150 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat ovary using mTOR antibody at dilution of 1:150 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using mTOR antibody at dilution of 1:150 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using mTOR antibody at dilution of 1:150 .)

IF (Immunofluorescence) (Immunofluorescence analysis of PC12 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using mTOR antibody at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using mTOR antibody at dilution of 1:150 .)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using mTOR antibody at dilution of 1:150 .)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using mTOR antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

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mTOR, Polyclonal Antibody (Cat# AAA30548)

Full Name

mTOR Polyclonal Antibody

Gene Names

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

IHC (Immunohistochemistry) ((4ug/ml) staining of paraffin embedded Human Lymph Node. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)

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ZCCHC11/PAPD3, Polyclonal Antibody (Cat# AAA13653)

Full Name

Goat anti-ZCCHC11/PAPD3 Antibody

Gene Names

ZCCHC11; TUT4; PAPD3

Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Applications

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$455/0.1 mg $875/2x0.1 mg $1,270/3x0.1 mg $1,645/4x0.1 mg $1,815/5x0.1 mg

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IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (CD276/B7-H3 Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CD276/B7-H3 Antibody-Western blot of recombinant CD276/B7-H3.)

WB (Western Blot) (CD276/B7-H3 Antibody-Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.)

WB (Western Blot) (CD276/B7-H3 Antibody-Knockout Varification: Lane 1: Wild-type 293T cell lysate; Lane 2: CD276 knockout 293T cell lysate; Predicted MW: 57,53,33kd Observed MW: 100kd Primary Ab: 3ug/ml Rabbit Anti-Human CD276 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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CD276/B7-H3, Polyclonal Antibody (Cat# AAA21333)

Full Name

CD276/B7-H3 Rabbit anti-Human Polyclonal (aa47-219) Antibody

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using EIF2AK4 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EIF2AK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using EIF2AK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using EIF2AK4 antibody at dilution of 1:100 (20x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using EIF2AK4 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using EIF2AK4 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HepG2 cells, using EIF2AK4 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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EIF2AK4, Polyclonal Antibody (Cat# AAA28254)

Full Name

EIF2AK4 Polyclonal Antibody

Gene Names

EIF2AK4; GCN2; PVOD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PARP1 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using PARP1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using PARP1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using PARP1 antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and PARP1 knockout (KO) HeLa cells, using PARP1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of Jurkat cells, using PARP1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PARP1 antibody.)

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PARP1, Polyclonal Antibody (Cat# AAA29790)

Full Name

PARP1 Polyclonal Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of U-937 cells, using NLRP3 antibody at 1:1000 dilution.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using NLRP3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using NLRP3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of rat lung cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

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NLRP3, Polyclonal Antibody (Cat# AAA30558)

Full Name

NLRP3 Polyclonal Antibody

Gene Names

NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (CD276/B7-H3 Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CD276/B7-H3 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

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CD276/B7-H3, Polyclonal Antibody (Cat# AAA21341)

Full Name

CD276/B7-H3 Rabbit anti-Human Polyclonal Antibody

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

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WB (Western Blot) (WB Suggested Anti-SOX2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:500Positive Control: OVCAR-3 cell lysateThere is BioGPS gene expression data showing that SOX2 is expressed in OVCAR3)

WB (Western Blot) (Sample Type: Lane 1: 20 ug U87 lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:2000Submitted by: Ander Matheu Fernandez, Biodonostia Institute)

WB (Western Blot) (Host: RabbitTarget Name: SOX2Sample Tissue: Mouse LiverAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: SOX2Sample Tissue: Human 293TAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: MouseTarget Name: SOX2Sample Tissue: Mouse PancreasAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: SOX2Sample Tissue: Mouse LiverAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Researcher:Kim Perry, University of IllinoisApplication:IHCSpecies+tissue/cell type:Xenopus laevis cornea epithelliumPrimary antibody dilution:1:300Secondary antibody:Goat anti-rabbit -rhodamineSecondary antibody dilution:1:300)

IHC (Immunohistochemistry) (Human Spleen)

IHC (Immunohistochemistry) (Human Kidney)

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SOX2, Polyclonal Antibody (Cat# AAA23389)

Full Name

SOX2 antibody - N-terminal region

Gene Names

SOX2; ANOP3; MCOPS3

Reactivity

Cow, Dog, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

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FAK, Polyclonal Antibody (Cat# AAA29021)

Full Name

FAK Polyclonal Antibody

Gene Names

PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

WB (Western Blot) ((1ug/ml) staining of Mouse Kidney lysate (A) and of Mouse Spleen lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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PBR, Polyclonal Antibody (Cat# AAA13662)

Full Name

Goat anti-PBR (mouse) Antibody

Gene Names

Tspo; IBP; PBR; Bzrp; Tspo1

Reactivity

Tested: Mouse; Expected from sequence similarity: Mouse, Rat

Applications

Peptide ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$455/0.1 mg $875/2x0.1 mg $1,270/3x0.1 mg $1,645/4x0.1 mg $1,815/5x0.1 mg

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitating TOM1 in HepG2 whole cell lysate .Western blot analysis of TOM1 using anti-TOM1 antibody (AAA19807).Lane 1: HepG2 whole cell lysates (30ug),Lane 2: Rabbit control IgG instead of anti-TOM1 antibody in HepG2 whole cell lysate,Lane 3: anti-TOM1 antibody (2ug) + HepG2 whole cell lysate (500ug).After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TOM1 antigen affinity purified polyclonal antibody (AAA19807) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light chain). The signal is developed using ECL Plus Western Blotting Substrate . A specific band was detected for TOM1 at approximately 54 kDa. The expected band size for TOM1 is at 54 kDa.)

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of Hela cells using anti-TOM1 antibody (AAA19807).Overlay histogram showing Hela cells stained with AAA19807 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TOM1 Antibody (AAA19807, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of TOM1 using anti-TOM1 antibody (AAA19807).TOM1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TOM1 Antibody (AAA19807) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-TOM1 Antibody (AAA19807) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOM1 antigen affinity purified polyclonal antibody (#AAA19807) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TOM1 at approximately 54 kDa. The expected band size for TOM1 is at 54 kDa.)

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TOM1, Polyclonal Antibody (Cat# AAA19807)

Full Name

Anti-TOM1 Antibody Picoband

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.