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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1150-1200 of 96811 product results
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-TOMM40 antibody (AAA19478).Overlay histogram showing MCF-7 cells stained with AAA19478 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM40 Antibody (AAA19478, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TOMM40, Polyclonal Antibody (Cat# AAA19478)
Full Name
Anti-TOMM40 Antibody Picoband
Gene Names
TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
PSG1, Polyclonal Antibody (Cat# AAA29196)
Full Name
PSG1 Polyclonal Antibody
Gene Names
PSG1; SP1; B1G1; PBG1; CD66f; PSBG1; PSG95; PSGGA; DHFRP2; PSBG-1; PSGIIA; FL-NCA-1/2; PS-beta-C/D; PS-beta-G-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
GCP5, Polyclonal Antibody (Cat# AAA29029)
Full Name
GCP5 Polyclonal Antibody
Gene Names
TUBGCP5; GCP5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
LC3A, Polyclonal Antibody (Cat# AAA31334)
Full Name
LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: ANP32A konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
ANP32A, Polyclonal Antibody (Cat# AAA27748)
Full Name
Anti-ANP32A Antibody, Rabbit Polyclonal
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using CDA Antibody at dilution of 1:100 (40x lens).)
CDA, Polyclonal Antibody (Cat# AAA28268)
Full Name
CDA Polyclonal Antibody
Gene Names
CDA; CDD
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using UMPS antibody.)
UMPS, Polyclonal Antibody (Cat# AAA28172)
Full Name
UMPS Polyclonal Antibody
Gene Names
UMPS; OPRT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Blank control: Molt-4 Cells(blue). Primary Antibody: Rabbit Anti-EPOR/FITC Conjugated antibody, Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG orange,used under the same conditions. Protocol: The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.)
EPOR, Polyclonal Antibody (Cat# AAA27968)
Full Name
EPOR
Reactivity
Human, Mouse, Rat, Dog, Cow, Horse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Affinity purified by Protein A
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)
Histone H4, Polyclonal Antibody (Cat# AAA28845)
Full Name
Histone H4 (Acetyl Lys16) Polyclonal Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Claudin-3, Polyclonal Antibody (Cat# AAA28995)
Full Name
Claudin-3 Polyclonal Antibody
Gene Names
CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HepG2 cell using CAPZA2 antibody. Blue: DAPI for nuclear staining)
CAPZA2, Polyclonal Antibody (Cat# AAA29715)
Full Name
CAPZA2 Antibody
Gene Names
CAPZA2; CAPZ; CAPPA2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
PPP1R15B, Polyclonal Antibody (Cat# AAA29301)
Full Name
PPP1R15B Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using SDC2 antibody at dilution of 1:100 (40x lens).)
SDC2, Polyclonal Antibody (Cat# AAA10698)
Full Name
SDC2 Polyclonal Antibody
Gene Names
SDC2; HSPG; CD362; HSPG1; SYND2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-FXR1 antibody (AAA19484).Overlay histogram showing A431 cells stained with AAA19484 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FXR1 Antibody (AAA19484, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FXR1, Polyclonal Antibody (Cat# AAA19484)
Full Name
Anti-FXR1 Antibody Picoband
Gene Names
FXR1; FXR1P
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using NDUFA4 Polyclonal Antibody.)
NDUFA4, Polyclonal Antibody (Cat# AAA30632)
Full Name
NDUFA4 Rabbit Polyclonal Antibody
Gene Names
NDUFA4; MLRQ; CI-9k; CI-MLRQ
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using FLNA at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
FLNA, Polyclonal Antibody (Cat# AAA30641)
Full Name
FLNA Rabbit Polyclonal Antibody
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody at dilution of 1:200 .)
S100A8, Polyclonal Antibody (Cat# AAA30576)
Full Name
S100A8 Polyclonal Antibody
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Anti-GPR89A antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
GPR89A, Polyclonal Antibody (Cat# AAA12327)
Full Name
Rabbit Polyclonal to Human GPR89A
Gene Names
GPR89A; GPHR; GPR89; SH120; GPR89B; UNQ192
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat, Zebrafish
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using BRCA1 antibody. Blue: DAPI for nuclear staining.)
BRCA1, Polyclonal Antibody (Cat# AAA29702)
Full Name
BRCA1 Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
WB (Western Blot)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
Caveolin-1, Polyclonal Antibody (Cat# AAA28981)
Full Name
Caveolin-1 Polyclonal Antibody
Gene Names
CAV1; CGL3; BSCL3; VIP21; MSTP085
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Cleaved-Caspase-6 p18, Polyclonal Antibody (Cat# AAA28831)
Full Name
Cleaved-Caspase-6 p18 (D162) Polyclonal Antibody
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Anti-GLUT4 Picoband antibody, AAA11601-6.JPGIHC(P): Mouse Skeletal Muscle Tissue)
GLUT4, Polyclonal Antibody (Cat# AAA11601)
Full Name
Anti-GLUT4 Antibody
Gene Names
SLC2A4; GLUT4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-Serum Response Factor/SRF antibody (AAA19221).Overlay histogram showing U251 cells stained with AAA19221 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serum Response Factor/SRF Antibody (AAA19221, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Serum Response Factor/SRF, Polyclonal Antibody (Cat# AAA19221)
Full Name
Anti-Serum Response Factor/SRF Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HCT116 cells using anti-SRPRA antibody (AAA19972).Overlay histogram showing HCT116 cells stained with AAA19972 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRA Antibody (AAA19972, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SRPRA, Polyclonal Antibody (Cat# AAA19972)
Full Name
Anti-SRPRA Antibody Picoband
Gene Names
SRPR; DP; Sralpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Asparagine synthetase/ASNS antibody (AAA19789).Overlay histogram showing MCF-7 cells stained with AAA19789 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Asparagine synthetase/ASNS Antibody (AAA19789, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Asparagine synthetase/ASNS, Polyclonal Antibody (Cat# AAA19789)
Full Name
Anti-Asparagine synthetase/ASNS Antibody Picoband
Gene Names
ASNS; TS11; ASNSD
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
PKC, Polyclonal Antibody (Cat# AAA29099)
Full Name
PKC Polyclonal Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
TSLC1, Polyclonal Antibody (Cat# AAA29134)
Full Name
TSLC1 Polyclonal Antibody
Gene Names
CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Catenin-alpha1, Polyclonal Antibody (Cat# AAA28979)
Full Name
Catenin-alpha1 Polyclonal Antibody
Gene Names
CTNNA1; CAP102
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
Cytokeratin 18, Polyclonal Antibody (Cat# AAA29009)
Full Name
Cytokeratin 18 Polyclonal Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ADSS. Blue: DAPI for nuclear staining.)
ADSS, Polyclonal Antibody (Cat# AAA30602)
Full Name
ADSS Rabbit Polyclonal Antibody
Gene Names
ADSS; ADEH; ADSS 2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using Nono Antibody at dilution of 1:100 (40x lens).)
Nono, Polyclonal Antibody (Cat# AAA28188)
Full Name
Nono Polyclonal Antibody
Gene Names
NONO; P54; NMT55; NRB54; P54NRB; PPP1R114
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using HIST1H2AG antibody at dilution of 1:100 (40x lens).)
HIST1H2AG, Polyclonal Antibody (Cat# AAA28259)
Full Name
HIST1H2AG Polyclonal Antibody
Gene Names
HIST1H2AG; H2AG; H2A/p; H2AFP; H2A.1b; pH2A/f
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26898)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Purified by Immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using MKI67 antibody.)
MKI67, Polyclonal Antibody (Cat# AAA28242)
Full Name
MKI67 Polyclonal Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Flk-1, Polyclonal Antibody (Cat# AAA29024)
Full Name
Flk-1 Polyclonal Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA29653 (DRD1 Antibody) at dilution 1/50, on the right is treated with the synthetic peptide.)
DRD1, Polyclonal Antibody (Cat# AAA29653)
Full Name
DRD1 Antibody
Gene Names
DRD1; DADR; DRD1A
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
N4BP1, Polyclonal Antibody (Cat# AAA29078)
Full Name
N4BP1 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
ZC3H12A, Polyclonal Antibody (Cat# AAA11042)
Full Name
ZC3H12A (CT) Antibody
Gene Names
ZC3H12A; MCPIP; MCPIP1; dJ423B22.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
ZC3H12A Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescence of Clusterin in mouse brain tissue with Clusterin Antibody at 20 μg/mL.Green: Clusterin Antibody (3856)Red: Phylloidin stainingBlue: DAPI staining)
Clusterin, Polyclonal Antibody (Cat# AAA10914)
Full Name
Clusterin Antibody
Gene Names
CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Clusterin Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human tonsil stained with a-1-Antitrypsin (AAT) antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of endothelial cells.)
alpha 1 Antitrypsin, Polyclonal Antibody (Cat# AAA14320)
Full Name
alpha 1 Antitrypsin antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human
Applications
Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using AMPKalpha1 at 1:1000 dilution.)
AMPKalpha1, Polyclonal Antibody (Cat# AAA30645)
Full Name
AMPKalpha1 Rabbit Polyclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MARK2, Polyclonal Antibody (Cat# AAA31392)
Full Name
Phospho-MARK2 (Thr596) Antibody
Gene Names
MARK2; EMK1; EMK-1; PAR-1; Par1b; Par-1b
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
TOPBP1, Polyclonal Antibody (Cat# AAA31285)
Full Name
Phospho-TOPBP1 (Ser1138) Antibody
Gene Names
TOPBP1; TOP2BP1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HepG2 cells using anti-PCCA antibody (AAA19790).Overlay histogram showing HepG2 cells stained with AAA19790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCCA Antibody (AAA19790, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCCA, Polyclonal Antibody (Cat# AAA19790)
Full Name
Anti-PCCA Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of 293T cells using anti-MFAP3 antibody (AAA19989).Overlay histogram showing 293T cells stained with AAA19989 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MFAP3 Antibody (AAA19989, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MFAP3, Polyclonal Antibody (Cat# AAA19989)
Full Name
Anti-MFAP3 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of CBX1/HP1 beta using anti-CBX1/HP1 beta antibody (AAA19508).CBX1/HP1 beta was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-CBX1/HP1 beta Antibody (AAA19508) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CBX1/HP1 beta, Polyclonal Antibody (Cat# AAA19508)
Full Name
Anti-CBX1/HP1 beta Antibody Picoband
Gene Names
CBX1; CBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity purified.
Pricing
ICC (Immunocytochemistry)
(ICC staining catalase in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Catalase, Polyclonal Antibody (Cat# AAA29962)
Full Name
Catalase Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Peptide affinity purified
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)
NLRX1, Polyclonal Antibody (Cat# AAA29337)
Full Name
NLRX1 Polyclonal Antibody
Gene Names
NLRX1; NOD5; NOD9; NOD26; DLNB26; CLR11.3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
BAG3, Polyclonal Antibody (Cat# AAA31421)
Full Name
Phospho-BAG3 (Tyr457) Antibody
Gene Names
BAG3; BIS; BAG-3; CAIR-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)
DGAT2L3, Polyclonal Antibody (Cat# AAA29011)
Full Name
DGAT2L3 Polyclonal Antibody
Gene Names
AWAT1; DGA2; DGAT2L3
Reactivity
Human
Applications
Western Blot
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.