Filters
▼Clonality
▼Type
▼Reactivity
▼Gene Name
▼Isotype
▼Host
▼Application
▼Clone
▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1150-1200 of 10000 product results
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of SiHa cells using anti-Glutaminase/GLS antibody (AAA19239).Overlay histogram showing SiHa cells stained with AAA19239 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutaminase/GLS Antibody (AAA19239,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Glutaminase/GLS, Polyclonal Antibody (Cat# AAA19239)
Full Name
Anti-Glutaminase/GLS Antibody
Gene Names
GLS; GAC; GAM; KGA; GLS1; AAD20
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of THP-1 cells using anti-RPL11 antibody (AAA19783).Overlay histogram showing THP-1 cells stained with AAA19783 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL11 Antibody (AAA19783, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
RPL11, Polyclonal Antibody (Cat# AAA19783)
Full Name
Anti-RPL11 Antibody Picoband
Gene Names
RPL11; L11; DBA7; GIG34
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(KRT8/CK8/Cytokeratin 8 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
KRT8/CK8/Cytokeratin 8, Polyclonal Antibody (Cat# AAA21348)
Full Name
KRT8/CK8/Cytokeratin 8 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of human colon carcinoma using Galectin 3/Galectin 3/LGALS3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Galectin 3/LGALS3, Polyclonal Antibody (Cat# AAA22299)
Full Name
Galectin 3/LGALS3 Polyclonal Antibody
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of SH-SY5Y cells using NLRP1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
NLRP1, Polyclonal Antibody (Cat# AAA22302)
Full Name
NLRP1 Polyclonal Antibody
Gene Names
NLRP1; NAC; CARD7; CIDED; NALP1; SLEV1; DEFCAP; PP1044; VAMAS1; CLR17.1; DEFCAP-L/S
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat pancreas using IRE1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IRE1, Polyclonal Antibody (Cat# AAA22308)
Full Name
IRE1 Polyclonal Antibody
Gene Names
ERN1; IRE1; IRE1P; IRE1a; hIRE1p
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinantprotein (80ng, 40ng, 20ng, 10ng)All lanes: Human IgG Fc antibody;HRPconjugated at 1:1000Predicted band size: 35 kDaObserved band size: 35 kDa)
IgG Fc fragment, Polyclonal Secondary Antibody (Cat# AAA15949)
Full Name
Rabbit anti-Human IgG Fc fragment polyclonal Antibody, HRP conjugated
Reactivity
Human
Applications
EIA, WB
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using SPARC antibody at dilution of 1:100 (40x lens).)
SPARC, Polyclonal Antibody (Cat# AAA10683)
Full Name
SPARC Polyclonal Antibody
Gene Names
SPARC; ON
Applications
Immunohistochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of SiHa cells using anti-MFF antibody (AAA19464).Overlay histogram showing SiHa cells stained with AAA19464 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MFF Antibody (AAA19464, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MFF, Polyclonal Antibody (Cat# AAA19464)
Full Name
Anti-MFF Antibody Picoband
Gene Names
MFF; EMPF2; GL004; C2orf33
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-HHEX antibody Titration: 1 ug/mLSample Type: Human HepG2)
HHEX, Polyclonal Antibody (Cat# AAA23384)
Full Name
HHEX antibody - middle region
Gene Names
HHEX; HEX; PRH; HMPH; PRHX; HOX11L-PEN
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(HMG-CoA Reductase/HMGCR Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
HMG-CoA Reductase/HMGCR, Polyclonal Antibody (Cat# AAA21345)
Full Name
HMG-CoA Reductase/HMGCR Rabbit anti-Human Polyclonal Antibody
Gene Names
HMGCR; LDLCQ3
Reactivity
Mouse, Rat, Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody. Western blot was performed from the immunoprecipitate using SUZ12 antibody at a dilition of 1:500.)
SUZ12, Polyclonal Antibody (Cat# AAA28195)
Full Name
SUZ12 Polyclonal Antibody
Gene Names
SUZ12; CHET9; JJAZ1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using [KO Validated] AKT1 Rabbit pAb (AAA28440) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
AKT1, Polyclonal Antibody (Cat# AAA28440)
Full Name
[KO Validated] AKT1 Rabbit pAb
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of extracts of rat brain, using HRH antibody (AAA28297) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:1000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s)
HRH3, Polyclonal Antibody (Cat# AAA28297)
Full Name
HRH3 Polyclonal Antibody
Gene Names
HRH3; HH3R; GPCR97
Reactivity
Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using TEFM antibody.)
TEFM, Polyclonal Antibody (Cat# AAA29856)
Full Name
TEFM Polyclonal Antibody
Gene Names
TEFM; C17orf42
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
PKC delta, Polyclonal Antibody (Cat# AAA31409)
Full Name
Phospho-PKC delta (Ser664) Antibody
Gene Names
PRKCD; MAY1; PKCD; nPKC-delta
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (82%), Bovine (91%), Horse (91%), Sheep (91%), Dog (91%), Chicken (91%), Xenopus (82%)
Predicted Reactivity: Pig (82%), Bovine (91%), Horse (91%), Sheep (91%), Dog (91%), Chicken (91%), Xenopus (82%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
INCENP, Polyclonal Antibody (Cat# AAA31457)
Full Name
Phospho-INCENP (Thr59) Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Horse (100%), Sheep (89%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Stat1, Polyclonal Antibody (Cat# AAA29124)
Full Name
Stat1 Polyclonal Antibody
Gene Names
STAT1; CANDF7; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of Raw264.7 cells using eEF1A1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
eEF1A1, Polyclonal Antibody (Cat# AAA28354)
Full Name
eEF1A1 Rabbit pAb
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of rat kidney using CDH16 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
CDH16, Polyclonal Antibody (Cat# AAA30698)
Full Name
CDH16 Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of A-431 cells, using CREB1 antibody at 1:1000 dilution.)
CREB1, Polyclonal Antibody (Cat# AAA30545)
Full Name
CREB1 Polyclonal Antibody
Gene Names
CREB1; CREB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using FAK antibody at dilution of 1:100 .)
FAK, Polyclonal Antibody (Cat# AAA30546)
Full Name
FAK Polyclonal Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-TOMM40 antibody (AAA19478).Overlay histogram showing MCF-7 cells stained with AAA19478 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM40 Antibody (AAA19478, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TOMM40, Polyclonal Antibody (Cat# AAA19478)
Full Name
Anti-TOMM40 Antibody Picoband
Gene Names
TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
PSG1, Polyclonal Antibody (Cat# AAA29196)
Full Name
PSG1 Polyclonal Antibody
Gene Names
PSG1; SP1; B1G1; PBG1; CD66f; PSBG1; PSG95; PSGGA; DHFRP2; PSBG-1; PSGIIA; FL-NCA-1/2; PS-beta-C/D; PS-beta-G-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
GCP5, Polyclonal Antibody (Cat# AAA29029)
Full Name
GCP5 Polyclonal Antibody
Gene Names
TUBGCP5; GCP5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
LC3A, Polyclonal Antibody (Cat# AAA31334)
Full Name
LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
WB (Western Blot)
(Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: ANP32A konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
ANP32A, Polyclonal Antibody (Cat# AAA27748)
Full Name
Anti-ANP32A Antibody, Rabbit Polyclonal
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using CDA Antibody at dilution of 1:100 (40x lens).)
CDA, Polyclonal Antibody (Cat# AAA28268)
Full Name
CDA Polyclonal Antibody
Gene Names
CDA; CDD
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using UMPS antibody.)
UMPS, Polyclonal Antibody (Cat# AAA28172)
Full Name
UMPS Polyclonal Antibody
Gene Names
UMPS; OPRT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Blank control: Molt-4 Cells(blue). Primary Antibody: Rabbit Anti-EPOR/FITC Conjugated antibody, Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG orange,used under the same conditions. Protocol: The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.)
EPOR, Polyclonal Antibody (Cat# AAA27968)
Full Name
EPOR
Reactivity
Human, Mouse, Rat, Dog, Cow, Horse
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Affinity purified by Protein A
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)
Histone H4, Polyclonal Antibody (Cat# AAA28845)
Full Name
Histone H4 (Acetyl Lys16) Polyclonal Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Claudin-3, Polyclonal Antibody (Cat# AAA28995)
Full Name
Claudin-3 Polyclonal Antibody
Gene Names
CLDN3; RVP1; HRVP1; C7orf1; CPE-R2; CPETR2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HepG2 cell using CAPZA2 antibody. Blue: DAPI for nuclear staining)
CAPZA2, Polyclonal Antibody (Cat# AAA29715)
Full Name
CAPZA2 Antibody
Gene Names
CAPZA2; CAPZ; CAPPA2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
PPP1R15B, Polyclonal Antibody (Cat# AAA29301)
Full Name
PPP1R15B Polyclonal Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human stomach using SDC2 antibody at dilution of 1:100 (40x lens).)
SDC2, Polyclonal Antibody (Cat# AAA10698)
Full Name
SDC2 Polyclonal Antibody
Gene Names
SDC2; HSPG; CD362; HSPG1; SYND2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-FXR1 antibody (AAA19484).Overlay histogram showing A431 cells stained with AAA19484 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FXR1 Antibody (AAA19484, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FXR1, Polyclonal Antibody (Cat# AAA19484)
Full Name
Anti-FXR1 Antibody Picoband
Gene Names
FXR1; FXR1P
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using NDUFA4 Polyclonal Antibody.)
NDUFA4, Polyclonal Antibody (Cat# AAA30632)
Full Name
NDUFA4 Rabbit Polyclonal Antibody
Gene Names
NDUFA4; MLRQ; CI-9k; CI-MLRQ
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using FLNA at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
FLNA, Polyclonal Antibody (Cat# AAA30641)
Full Name
FLNA Rabbit Polyclonal Antibody
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using S100A8 antibody at dilution of 1:200 .)
S100A8, Polyclonal Antibody (Cat# AAA30576)
Full Name
S100A8 Polyclonal Antibody
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Anti-GPR89A antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
GPR89A, Polyclonal Antibody (Cat# AAA12327)
Full Name
Rabbit Polyclonal to Human GPR89A
Gene Names
GPR89A; GPHR; GPR89; SH120; GPR89B; UNQ192
Reactivity
Bat, Gibbon, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat, Zebrafish
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cell using BRCA1 antibody. Blue: DAPI for nuclear staining.)
BRCA1, Polyclonal Antibody (Cat# AAA29702)
Full Name
BRCA1 Antibody
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
WB (Western Blot)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
Caveolin-1, Polyclonal Antibody (Cat# AAA28981)
Full Name
Caveolin-1 Polyclonal Antibody
Gene Names
CAV1; CGL3; BSCL3; VIP21; MSTP085
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
Application Data
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Cleaved-Caspase-6 p18, Polyclonal Antibody (Cat# AAA28831)
Full Name
Cleaved-Caspase-6 p18 (D162) Polyclonal Antibody
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Anti-GLUT4 Picoband antibody, AAA11601-6.JPGIHC(P): Mouse Skeletal Muscle Tissue)
GLUT4, Polyclonal Antibody (Cat# AAA11601)
Full Name
Anti-GLUT4 Antibody
Gene Names
SLC2A4; GLUT4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-Serum Response Factor/SRF antibody (AAA19221).Overlay histogram showing U251 cells stained with AAA19221 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Serum Response Factor/SRF Antibody (AAA19221, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Serum Response Factor/SRF, Polyclonal Antibody (Cat# AAA19221)
Full Name
Anti-Serum Response Factor/SRF Antibody
Gene Names
SRF; MCM1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HCT116 cells using anti-SRPRA antibody (AAA19972).Overlay histogram showing HCT116 cells stained with AAA19972 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SRPRA Antibody (AAA19972, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
SRPRA, Polyclonal Antibody (Cat# AAA19972)
Full Name
Anti-SRPRA Antibody Picoband
Gene Names
SRPR; DP; Sralpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Asparagine synthetase/ASNS antibody (AAA19789).Overlay histogram showing MCF-7 cells stained with AAA19789 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Asparagine synthetase/ASNS Antibody (AAA19789, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Asparagine synthetase/ASNS, Polyclonal Antibody (Cat# AAA19789)
Full Name
Anti-Asparagine synthetase/ASNS Antibody Picoband
Gene Names
ASNS; TS11; ASNSD
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
PKC, Polyclonal Antibody (Cat# AAA29099)
Full Name
PKC Polyclonal Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
TSLC1, Polyclonal Antibody (Cat# AAA29134)
Full Name
TSLC1 Polyclonal Antibody
Gene Names
CADM1; BL2; ST17; IGSF4; NECL2; RA175; TSLC1; IGSF4A; Necl-2; SYNCAM; sgIGSF; sTSLC-1; synCAM1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Catenin-alpha1, Polyclonal Antibody (Cat# AAA28979)
Full Name
Catenin-alpha1 Polyclonal Antibody
Gene Names
CTNNA1; CAP102
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.