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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1050-1100 of 3176 product results
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
HNRNPA0, Polyclonal Antibody (Cat# AAA31447)
Full Name
Phospho-HNRNPA0 (Ser84) Antibody
Gene Names
HNRNPA0; HNRPA0
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of U20S cells using anti-Dynamin 1 antibody (AAA19160).Overlay histogram showing U20S cells stained with AAA19160 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1 Antibody (AAA19160,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Dynamin 1, Polyclonal Antibody (Cat# AAA19160)
Full Name
Anti-Dynamin 1 Picoband antibody
Gene Names
DNM1; DNM; EIEE31
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
L-Selectin, Polyclonal Antibody (Cat# AAA29150)
Full Name
L-Selectin Polyclonal Antibody
Gene Names
SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)
KV4.3, Polyclonal Antibody (Cat# AAA29939)
Full Name
KV4.3 Antibody
Gene Names
KCND3; KV4.3; SCA19; SCA22; KCND3L; KCND3S; KSHIVB
Reactivity
Human, Mouse
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U937 cells using anti-Syntaxin 18/STX18 antibody (AAA19967).Overlay histogram showing U937 cells stained with AAA19967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Syntaxin 18/STX18 Antibody (AAA19967, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Syntaxin 18/STX18, Polyclonal Antibody (Cat# AAA19967)
Full Name
Anti-Syntaxin 18/STX18 Antibody Picoband
Gene Names
STX18; Ufe1
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)
Smad1, Polyclonal Antibody (Cat# AAA28413)
Full Name
[KO Validated] Smad1 Rabbit pAb
Reactivity
Human
Applications
WB, IHC, IF, ICC
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.)
Heme Oxygenase 1 (HO-1/HMOX1), Polyclonal Antibody (Cat# AAA28414)
Full Name
[KO Validated] Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb
Gene Names
NR1D2; RVR; BD73; EAR-1R
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of C6 cells using anti-hnRNP U/p120/HNRNPU antibody (AAA19498).Overlay histogram showing C6 cells stained with AAA19498 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP U/p120/HNRNPU Antibody (AAA19498, 1 ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
hnRNP U/p120/HNRNPU, Polyclonal Antibody (Cat# AAA19498)
Full Name
Anti-hnRNP U/p120/HNRNPU Antibody Picoband
Gene Names
HNRNPU; HNRPU; SAF-A; U21.1; hnRNP U
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U87 cells using anti-RAB13 antibody (AAA19513).Overlay histogram showing U87 cells stained with AAA19513 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB13 Antibody (AAA19513, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RAB13, Polyclonal Antibody (Cat# AAA19513)
Full Name
Anti-RAB13 Antibody Picoband
Gene Names
RAB13; GIG4
Reactivity
Human, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of PC-3 cells using anti-PAK3 antibody (AAA19477).Overlay histogram showing PC-3 cells stained with AAA19477 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAK3 Antibody (AAA19477, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PAK3, Polyclonal Antibody (Cat# AAA19477)
Full Name
Anti-PAK3 Antibody Picoband
Gene Names
PAK3; bPAK; MRX30; MRX47; OPHN3; hPAK3; CDKN1A; PAK3beta
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of Caco-2 cells using anti-TOMM70 antibody (AAA19606).Overlay histogram showing Caco-2 cells stained with AAA19606 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOMM70 Antibody (AAA19606, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TOMM70, Polyclonal Antibody (Cat# AAA19606)
Full Name
Anti-TOMM70 Antibody Picoband
Reactivity
Human
Applications
EIA, FC/FACS, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-NDUFB5 antibody (AAA19333).Overlay histogram showing A431 cells stained with AAA19333 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB5 Antibody (AAA19333, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFB5, Polyclonal Antibody (Cat# AAA19333)
Full Name
Anti-NDUFB5 Antibody
Gene Names
NDUFB5; SGDH; CISGDH
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of Hela cells using anti- ETF/TEAD2 antibody (AAA19325).Overlay histogram showing Hela cells stained withAAA19325 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- ETF/TEAD2 Antibody (AAA19325, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ETF/TEAD2, Polyclonal Antibody (Cat# AAA19325)
Full Name
Anti-ETF/TEAD2 Antibody
Gene Names
TEAD2; ETF; TEF4; TEF-4; TEAD-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 12. Flow Cytometry analysis of 293T cells using anti-ATP5F1B antibody (AAA19652).Overlay histogram showing 293T cells stained with AAA19652 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5F1B Antibody (AAA19652, 1 ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ATP5F1B, Polyclonal Antibody (Cat# AAA19652)
Full Name
Anti-ATP5F1B Antibody Picoband
Gene Names
ATP5F1B; ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Uterine Fibroids Tissue)
Chymase 1, Mast Cell (CMA1), Polyclonal Antibody (Cat# AAA20093)
Full Name
Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)
Gene Names
Cma1; Mcp5; Mcp-5; Mcpt5; MMCP-5
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using IFIH1 Rabbit pAb.)
IFIH1, Polyclonal Antibody (Cat# AAA30724)
Full Name
IFIH1 Rabbit Polyclonal Antibody
Gene Names
IFIH1; Hlcd; MDA5; MDA-5; RLR-2; IDDM19
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26900)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of GCLC using anti-GCLC antibody (AAA19759).GCLC was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-GCLC Antibody (AAA19759) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
GCLC, Polyclonal Antibody (Cat# AAA19759)
Full Name
Anti-GCLC Antibody Picoband
Gene Names
GCLC; GCL; GCS; GLCL; GLCLC
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
OTUB1, Polyclonal Antibody (Cat# AAA31284)
Full Name
Phospho-OTUB1 (Ser16) Antibody
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Anti-ANP32A rabbit polyclonal antibody at 1:500 dilution Lane A: ANP32A konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa Observed band size:28 kDa)
ANP32A, Polyclonal Antibody (Cat# AAA27748)
Full Name
Anti-ANP32A Antibody, Rabbit Polyclonal
Gene Names
ANP32A; LANP; MAPM; PP32; HPPCn; PHAP1; PHAPI; I1PP2A; C15orf1
Reactivity
Human
Applications
WB, EIA, IHC-P, ICC, IF, IP
Purity
Protein A & Antigen Affinity
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spinal cord using HYOU1 antibody at dilution of 1:100 (40x lens).)
HYOU1, Polyclonal Antibody (Cat# AAA28092)
Full Name
HYOU1 Polyclonal Antibody
Gene Names
HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
Reactivity
Human
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-RBM26 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that RBM26 is expressed in HepG2)
RBM26, Polyclonal Antibody (Cat# AAA23487)
Full Name
RBM26 antibody - middle region
Gene Names
RBM26; ARRS2; SE70-2; ZC3H17; PRO1777; C13orf10; PPP1R132
Reactivity
Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
NF2, Polyclonal Antibody (Cat# AAA29080)
Full Name
NF2 Polyclonal Antibody
Gene Names
NF2; ACN; SCH; BANF
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using CTCF at dilution of 1:100. Blue: DAPI for nuclear staining.)
CTCF, Polyclonal Antibody (Cat# AAA30612)
Full Name
CTCF Rabbit Polyclonal Antibody
Gene Names
CTCF; MRD21
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ChIP
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(HnRNP A1 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- HnRNP A1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
HnRNP A1, Polyclonal Antibody (Cat# AAA11683)
Full Name
Anti-HnRNP A1 Antibody
Gene Names
HNRNPA1; UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 8. IF analysis of ATP4B using anti-ATP4B antibody (AAA19909).ATP4B was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-ATP4B Antibody (AAA19909) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
ATP4B, Polyclonal Antibody (Cat# AAA19909)
Full Name
Anti-ATP4B Antibody Picoband
Gene Names
ATP4B; ATP6B
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat brain using RAB11A/RAB11B Rabbit pAb at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA28386)
Full Name
RAB11A/RAB11B Rabbit pAb
Gene Names
RAB11A; YL8
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of 293T cells using anti-IDH3G antibody (AAA19943).Overlay histogram showing 293T cells stained with AAA19943 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH3G Antibody (AAA19943, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IDH3G, Polyclonal Antibody (Cat# AAA19943)
Full Name
Anti-IDH3G Antibody Picoband
Gene Names
IDH3G; H-IDHG
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HepG2 cells using anti-MOGAT2 antibody (AAA19953).Overlay histogram showing HepG2 cells stained with AAA19953 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOGAT2 Antibody (AAA19953, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MOGAT2, Polyclonal Antibody (Cat# AAA19953)
Full Name
Anti-MOGAT2 Antibody Picoband
Gene Names
MOGAT2; MGAT2; DGAT2L5
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, ICC, WB, FC/FACS
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-HIF-1 alpha/HIF1A antibody (AAA19213).Overlay histogram showing A431 cells stained with AAA19213 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF-1 alpha/HIF1A Antibody (AAA19213, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HIF-1 alpha/HIF1A, Polyclonal Antibody (Cat# AAA19213)
Full Name
Anti-HIF-1 alpha/HIF1A Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of SiHa cells using anti-DRP1/DNM1L antibody (AAA19220).Overlay histogram showing SiHa cells stained with AAA19220 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRP1/DNM1L Antibody (AAA19220, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DRP1/DNM1L, Polyclonal Antibody (Cat# AAA19220)
Full Name
Anti-DRP1/DNM1L Antibody
Gene Names
DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
FoxO3A, Polyclonal Antibody (Cat# AAA28927)
Full Name
FoxO3A (phospho Ser253) Polyclonal Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Mouse Ovary Tissue.)
Microsomal Glutathione S Transferase 1, Polyclonal Antibody (Cat# AAA20838)
Full Name
Polyclonal Antibody to Microsomal Glutathione S Transferase 1 (MGST1)
Gene Names
Mgst1; Gst; 1500002K10Rik
Reactivity
Mouse, Rat
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: FAM108A1Sample Type: Human JurkatAntibody Dilution: 1.0ug/mlABHD17A is supported by BioGPS gene expression data to be expressed in Jurkat)
FAM108A1, Polyclonal Antibody (Cat# AAA23599)
Full Name
FAM108A1 Antibody - C-terminal region
Gene Names
ABHD17A; C19orf27; FAM108A1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse heart using Nono Antibody at dilution of 1:100 (40x lens).)
Nono, Polyclonal Antibody (Cat# AAA28188)
Full Name
Nono Polyclonal Antibody
Gene Names
NONO; P54; NMT55; NRB54; P54NRB; PPP1R114
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
VGF, Polyclonal Antibody (Cat# AAA29207)
Full Name
VGF Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Figure 6. IF analysis of Phox/NCF1 using anti-Phox/NCF1 antibody (AAA19752).Phox/NCF1 was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-Phox/NCF1 Antibody (AAA19752) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
p47 phox/NCF1, Polyclonal Antibody (Cat# AAA19752)
Full Name
Anti-p47 phox/NCF1 Antibody Picoband
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
LC3A, Polyclonal Antibody (Cat# AAA31334)
Full Name
LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody.)
ZP3, Polyclonal Antibody (Cat# AAA29805)
Full Name
ZP3 Polyclonal Antibody
Gene Names
ZP3; ZPC; ZP3A; ZP3B; Zp-3
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity purification
Pricing
ICC (Immunocytochemistry)
(Immunocytochemistry of ZIPK in Jurkat cells with ZIPK antibody at 10 μg/mL.)
ZIPK, Polyclonal Antibody (Cat# AAA10927)
Full Name
ZIPK Antibody
Gene Names
DAPK3; DLK; ZIP; ZIPK
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IF
Purity
ZIPK Antibody is affinity chromatography purified via peptide column.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using FLNB antibody.)
FLNB, Polyclonal Antibody (Cat# AAA29827)
Full Name
FLNB Polyclonal Antibody
Gene Names
FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Laminin alpha 5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Laminin alpha 5, Polyclonal Antibody (Cat# AAA17816)
Full Name
Anti-Laminin alpha 5 Antibody
Reactivity
Human, Mouse
Applications
WB, IHC, IP
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin Immunoprecipitation 293T(1.6^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-MCM3 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter.)
DNA replication licensing factor MCM3, Polyclonal Antibody (Cat# AAA18870)
Full Name
Rabbit anti-human DNA replication licensing factor MCM3 polyclonal Antibody(MCM3)
Gene Names
MCM3; HCC5; P1.h; RLFB; P1-MCM3
Reactivity
Human
Applications
EIA, WB, IHC, ChIP
Purity
Antigen Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of THP-1 cells using anti-CDC123 antibody (AAA19323).Overlay histogram showing THP-1 cells stained with AAA19323 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDC123 Antibody (AAA19323, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CDC123, Polyclonal Antibody (Cat# AAA19323)
Full Name
Anti-CDC123 Antibody
Gene Names
CDC123; D123; C10orf7
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of COX IV/COX4I1 using anti-COX IV/COX4I1 antibody (AAA19549).COX IV/COX4I1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-COX IV/COX4I1 Antibody (AAA19549) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
COX IV/COX4I1, Polyclonal Antibody (Cat# AAA19549)
Full Name
Anti-COX IV/COX4I1 Antibody Picoband
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of THP-1 cells using anti-GLUL antibody (AAA19479).Overlay histogram showing THP-1 cells stained with AAA19479 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLUL Antibody (AAA19479, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GLUL, Polyclonal Antibody (Cat# AAA19479)
Full Name
Anti-GLUL Antibody Picoband
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot analysis of FKBP4 expression in transfected 293T cell line by FKBP4 polyclonal antibody. Lane 1: FKBP4 transfected lysate (51.8kD). Lane 2: Non-transfected lysate.)
FKBP4, Polyclonal Antibody (Cat# AAA26871)
Full Name
FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (PE)
Gene Names
FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
Reactivity
Human, Mouse
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-GBP1 antibody (AAA19787).Overlay histogram showing MCF-7 cells stained with AAA19787 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GBP1 Antibody (AAA19787, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
GBP1, Polyclonal Antibody (Cat# AAA19787)
Full Name
Anti-GBP1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IP, IF, IHC, ICC, WB
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
NGF, Polyclonal Antibody (Cat# AAA29299)
Full Name
NGF Polyclonal Antibody
Gene Names
NGF; NGFB; HSAN5; Beta-NGF
Reactivity
Human
Applications
WB, IHC-P
Pricing
WB (Western Blot)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
JNK1/2/3, Polyclonal Antibody (Cat# AAA29056)
Full Name
JNK1/2/3 Polyclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.