Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (PSMA3 Antibody-Immunohistochemistry of paraffin-embedded rat lung using PSMA3 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry) (PSMA3 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (PSMA3 Antibody-Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (PSMA3 Antibody-Immunoprecipitation analysis of 200ug extracts of HL-60 cells, using 3 ug PSMA3 antibody. Western blot was performed from the immunoprecipitate using PSMA3 antibodyat a dilition of 1:1000.)

WB (Western Blot) (PSMA3 Antibody-Western blot analysis of extracts of various cell lines, using PSMA3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)

ICC (Immunocytochemistry) (PSMA3 Antibody-Immunofluorescence analysis of U2OS cells.)

ICC (Immunocytochemistry) (PSMA3 Antibody-Immunofluorescence analysis of MCF-7 cells using PSMA3 antibody. Blue: DAPI for nuclear staining.)

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PSMA3, Polyclonal Antibody (Cat# AAA21304)

Full Name

PSMA3 Rabbit anti-Human Polyclonal Antibody

Gene Names

PSMA3; HC8; PSC3

Reactivity

Mouse, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Affinity purified

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WB (Western Blot) (WB Suggested Anti-GABRP AntibodyTitration: 1.25 ug/mlPositive Control: HepG2/Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: GABRPSample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1.0ug/mLPeptide Concentration: 1.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 12%)

WB (Western Blot) (Host: RabbitTarget Name: GABRPSample Tissue: Human THP-1 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GABRPSample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GABRPSample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: GABRPSample Tissue: Human 293TAntibody Dilution: 1.0ug/ml)

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GABRP, Polyclonal Antibody (Cat# AAA23608)

Full Name

GABRP antibody - N-terminal region

Reactivity

Dog, Horse, Human, Mouse, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Protein A purified

Pricing

$445/0.1 mL $855/2x0.1 mL $1,245/3x0.1 mL $1,610/4x0.1 mL $1,800/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using METTL14 Rabbit pAb (AAA28216) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using METTL14 Rabbit pAb (AAA28216) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using METTL14 Rabbit pAb (AAA28216) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human colon using METTL14 Rabbit pAb (AAA28216) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using METTL14 Rabbit pAb (AAA28216) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using METTL14 Rabbit pAb (AAA28216) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug METTL14 antibody .Western blot was performed from the immunoprecipitate using METTL14 antibody (AAA28216) at a dilution of 1:1000.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using METTL14 antibody (AAA28216) at 1:730 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using METTL14 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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METTL14, Polyclonal Antibody (Cat# AAA28216)

Full Name

METTL14 Polyclonal Antibody

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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IFN-gammaRalpha, Polyclonal Antibody (Cat# AAA29240)

Full Name

IFN-gammaRalpha Polyclonal Antibody

Gene Names

IFNGR1; CD119; IFNGR

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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Application Data (Anti-mApple Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)

IF (Immunofluorescence) (Immunofluorescence: anti-mApple Ab using hCEC cells transduced with mApple-Rab5a; cells were fixed with methanol and anti-mApple at 1/250)

IF (Immunofluorescence) (Immunofluorescence: Anti-mApple Ab using hCEC cells transduced with mApple-Rab5a; cells were fixed with methanol and Anti-mApple at 1/250;)

Reactivity Chart

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mApple, Polyclonal Antibody (Cat# AAA13883)

Full Name

anti-mApple

Reactivity

Reacts with transfected cells proteins

Applications

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoelectron Microscopy (IEM)

Purity

This antibody is epitope-affinity purified from goat antiserum.

Pricing

$360/0.3 mg $690/2x0.3 mg $1,000/3x0.3 mg $1,295/4x0.3 mg $1,430/5x0.3 mg

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells usingug SHMT2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using SHMT2 Polyclonal Antibody at a dilution of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

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SHMT2, Polyclonal Antibody (Cat# AAA22331)

Full Name

SHMT2 Polyclonal Antibody

Gene Names

SHMT2; GLYA; SHMT; HEL-S-51e

Reactivity

Human

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

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CD79a, Polyclonal Antibody (Cat# AAA28987)

Full Name

CD79a Polyclonal Antibody

Gene Names

CD79A; IGA; MB-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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Jagged1, Polyclonal Antibody (Cat# AAA29243)

Full Name

Jagged1 Polyclonal Antibody

Gene Names

JAG1; AGS; AHD; AWS; HJ1; CD339; JAGL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

WB (Western Blot) (Western Blot analysis of Jurkat, L929 cells using EPAS-1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100)

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EPAS-1, Polyclonal Antibody (Cat# AAA30775)

Full Name

EPAS-1 Rabbit Polyclonal Antibody

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$305/0.05 mL $385/0.1 mL $740/2x0.1 mL $1,070/3x0.1 mL $1,385/4x0.1 mL $1,475/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from P19 cells(LPS treatment), using Phospho-LRRK2 (Ser935) Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from K562 cells(serum starvation treatment), using Phospho-LRRK2 (Ser935) Antibody. The lane on the left was treated with blocking peptide.)

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LRRK2, Polyclonal Antibody (Cat# AAA31287)

Full Name

Phospho-LRRK2 (Ser935) Antibody

Gene Names

LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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SRPK2, Polyclonal Antibody (Cat# AAA31293)

Full Name

Phospho-SRPK2 (Ser494/Ser497) Antibody

Gene Names

SRPK2; SFRSK2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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WB (Western Blot) (Anti-HDAC3 antibody, AAA11582, Western blottingLane 1: Rat Stomach Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: MCF-7 Cell LysateLane 4: HELA Cell LysateLane 5: JURKAT Cell LysateLane 6: SKOV Cell Lysate)

ICC (Immunocytochemistry) (Anti-HDAC3 antibody, AAA11582, ICCICC: A549 Cell)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(F)IHC(F): Mouse Brain Tissue)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

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HDAC3, Polyclonal Antibody (Cat# AAA11582)

Full Name

Anti-HDAC3 antibody

Gene Names

HDAC3; HD3; RPD3; RPD3-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (MAOA/Monoamine Oxidase Antibody-Immunohistochemistry of paraffin-embedded rat kidney using MAOA antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (MAOA/Monoamine Oxidase Antibody-Immunohistochemistry of paraffin-embedded mouse kidney using MAOA antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (MAOA/Monoamine Oxidase Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue.)

IHC (Immunohistochemistry) (MAOA/Monoamine Oxidase Antibody-Immunohistochemistry of paraffin-embedded rat spleen using MAOA antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (MAOA/Monoamine Oxidase Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (MAOA/Monoamine Oxidase Antibody-Western blot analysis of extracts of various cell lines, using MAOA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)

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MAOA/Monoamine Oxidase, Polyclonal Antibody (Cat# AAA21305)

Full Name

MAOA/Monoamine Oxidase Rabbit anti-Human Polyclonal Antibody

Gene Names

MAOA; MAO-A

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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EEF1A2, Polyclonal Antibody (Cat# AAA31289)

Full Name

Phospho-EEF1A2 (Ser358) Antibody

Gene Names

EEF1A2; HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in rat liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IP (Immunoprecipitation) (HMGB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate4 uL anti-HMGB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HMGB1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of HMGB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human HMGB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

WB (Western Blot) (Anti-HMGB1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)

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HMGB1, Polyclonal Antibody (Cat# AAA27719)

Full Name

Anti-HMGB1 Antibody, Rabbit Polyclonal

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

$185/0.02 mL $375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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COL2A1, Polyclonal Antibody (Cat# AAA28999)

Full Name

COL2A1 Polyclonal Antibody

Gene Names

COL2A1; AOM; ANFH; SEDC; COL11A3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of MCF7, K562 cells using Di-Methyl-p53 (K370) Polyclonal Antibody. Secondary antibody)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Immunofluorescence analysis of human-liver-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of human-kidney tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of human-breast-cancer tissue. 1,p53 (Di Methyl Lys370) Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

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p53, Polyclonal Antibody (Cat# AAA30791)

Full Name

p53 (Di Methyl Lys370) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Rat, Mouse

Applications

Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$305/0.05 mL $385/0.1 mL $740/2x0.1 mL $1,070/3x0.1 mL $1,385/4x0.1 mL $1,475/5x0.1 mL

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IP (Immunoprecipitation) (MAP2K3 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg HepG2 Whole Cell Lysate Lane C:0.5 mg HeLa Whole Cell Lysate 2 uL anti-MAP2K3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MAP2K3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :39 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of MAP2K3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MAP2K3 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining MAP2K3 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MAP2K3 in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MAP2K3 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining MAP2K3 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-MAP2K3 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:39 kDa Observed band size:39 kDa)

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MEK3/MKK3, Polyclonal Antibody (Cat# AAA27720)

Full Name

Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal

Gene Names

MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistochemistry) (TS/Thromboxane Synthase Antibody-Immunohistochemistry of paraffin-embedded human thyroid cancer using TBXAS1 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry) (TS/Thromboxane Synthase Antibody-Immunohistochemistry of paraffin-embedded human endometrial cancer using TBXAS1 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (TS/Thromboxane Synthase Antibody-Immunohistochemistry of paraffin-embedded mouse heart.)

IHC (Immunohistochemistry) (TS/Thromboxane Synthase Antibody-Immunohistochemistry of paraffin-embedded mouse lung using TBXAS1 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (TS/Thromboxane Synthase Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (TS/Thromboxane Synthase Antibody-Western blot analysis of extracts of various cell lines, using TBXAS1 antibody.)

ICC (Immunocytochemistry) (TS/Thromboxane Synthase Antibody-Immunofluorescence analysis of HeLa cell using TBXAS1 antibody. Blue: DAPI for nuclear staining.)

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TS/Thromboxane Synthase, Polyclonal Antibody (Cat# AAA21312)

Full Name

TS/Thromboxane Synthase Rabbit anti-Human Polyclonal Antibody

Gene Names

TBXAS1; TS; TXS; CYP5; THAS; TXAS; CYP5A1; GHOSAL; BDPLT14

Reactivity

Mouse, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Smarcd1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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BAF60a, Polyclonal Antibody (Cat# AAA28224)

Full Name

BAF60a Polyclonal Antibody

Gene Names

Smarcd1; Baf60a; D15Kz1; AA407987

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of EPHA4 (arrow) using EphA4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

WB (Western Blot) (Western blot analysis of lysates from Hela, NCI-H460, mouse NIH/3T3 cell line and human ovary tissue lysate (from left to right), using EPHA4 Antibody (R890). AAA28736 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from Hela, NCI-H460, HUVEC cell line (from left to right), using EPHA4 Antibody (R890). AAA28736 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using EPHA4 Antibody (R890). AAA28736 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

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EphA4, Polyclonal Antibody (Cat# AAA28736)

Full Name

EphA4 Antibody (C-term)

Gene Names

EPHA4; SEK; HEK8; TYRO1

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.05 mL $480/0.2 mL $925/2x0.2 mL $1,345/3x0.2 mL $1,740/4x0.2 mL $1,975/5x0.2 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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Rab8, Polyclonal Antibody (Cat# AAA31296)

Full Name

Phospho-Rab8 (Thr72) Antibody

Gene Names

RAB8A; MEL; RAB8

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human breast cancer using UBE2I antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry) (UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2I antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (UBE2I/UBC9 Antibody-Immunohistochemistry of paraffin-embedded rat lung tissue.)

IHC (Immunohistochemistry) (UBE2I/UBC9 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (UBE2I/UBC9 Antibody-Western blot analysis of extracts of various cell lines, using UBE2I antibody.)

WB (Western Blot) (UBE2I/UBC9 Antibody-Western blot analysis of extracts of various cell lines.)

ICC (Immunocytochemistry) (UBE2I/UBC9 Antibody-Immunofluorescence analysis of U2OS cell using UBE2I antibody. Blue: DAPI for nuclear staining.)

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UBE2I/UBC9, Polyclonal Antibody (Cat# AAA21313)

Full Name

UBE2I/UBC9 Rabbit anti-Human Polyclonal Antibody

Gene Names

UBE2I; P18; UBC9; C358B7.1

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistchemistry) (TCP1 Antibody-Immunohistochemistry of paraffin-embedded mouse testis using TCP1 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry) (TCP1 Antibody-Immunohistochemistry of paraffin-embedded rat kidney tissue.)

IHC (Immunohistochemistry) (TCP1 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (TCP1 Antibody-Western blot analysis of extracts of various cell lines, using TCP1 antibody.)

ICC (Immunocytochemistry) (TCP1 Antibody-Immunofluorescence analysis of U2OS cell using TCP1 antibody. Blue: DAPI for nuclear staining.)

ICC (Immunocytochemistry) (TCP1 Antibody-Immunofluorescence analysis of U2OS cells.)

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TCP1, Polyclonal Antibody (Cat# AAA21311)

Full Name

TCP1 Rabbit anti-Human Polyclonal Antibody

Gene Names

TCP1; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

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IHC (Immunohistochemistry) (Anti-VE Cadherin antibody, AAA11586, IHC(P)IHC(P): Rat Brain Tissue)

IHC (Immunohistochemistry) (Anti-VE Cadherin antibody, AAA11586, IHC(P)IHC(P): Human Lung Cancer Tissue)

WB (Western Blot) (Anti-VE Cadherin antibody, AAA11586, Western blottingAll lanes: Anti VE Cadherin (AAA11586) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 87KDObserved bind size: 130KD)

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VE Cadherin, Polyclonal Antibody (Cat# AAA11586)

Full Name

Polyclonal Anti-CDH5 Antibody; Anti-VE Cadherin antibody

Gene Names

CDH5; 7B4; CD144

Reactivity

Human, Rat; Predicted: Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (PSMA1 Antibody-Immunohistochemistry of paraffin-embedded mouse heart using PSMA1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistchemistry) (PSMA1 Antibody-Immunohistochemistry of paraffin-embedded human breast cancer using PSMA1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (PSMA1 Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using PSMA1 antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (PSMA1 Antibody-Immunohistochemistry of paraffin-embedded mouse lung tissue.)

IHC (Immunohistochemistry) (PSMA1 Antibody-Immunohistochemistry of paraffin-embedded rat lung using PSMA1 antibody at dilution of 1:100 (200x lens).)

IHC (Immunohistochemistry) (PSMA1 Antibody-Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (PSMA1 Antibody-Western blot analysis of extracts of various cell lines, using PSMA1 antibody.)

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PSMA1, Polyclonal Antibody (Cat# AAA21314)

Full Name

PSMA1 Rabbit anti-Human Polyclonal Antibody

Gene Names

PSMA1; NU; HC2; PROS30; HEL-S-275

Reactivity

Mouse, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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Tubulin alpha, Polyclonal Antibody (Cat# AAA29254)

Full Name

Tubulin alpha Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,Actin ? Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat-lung tissue. 1,Actin ? Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of human-uterus tissue. 1,Actin ? Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

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Actin beta, Polyclonal Antibody (Cat# AAA30790)

Full Name

Actin beta Antibody

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat, Chicken, Globefish, Bovine, Hamster, Pig, Ovine, Cat, Pig, Dog, Sheep

Applications

Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$290/0.1 mL $820/0.5 mL $1,310/1 mL $2,545/2x1 mL $3,710/3x1 mL $4,810/4x1 mL $5,625/5x1 mL

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IHC (Immunohistchemistry) (TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded rat kidney tissue at 1:200 dilution.)

IHC (Immunohistochemistry) (TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human kidney cancer tissue at 1:200 dilution.)

IHC (Immunohistochemistry) (TNFRSF6B/DCR3 Antibody-Immunohistochemistry of paraffin-embedded human normal stomach tissue at 1:200 dilution.)

IHC (Immunohistochemistry) (TNFRSF6B/DCR3 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of various cells.)

WB (Western Blot) (TNFRSF6B/DCR3 Antibody-Western blot analysis of extracts of HT-29 cells, using TNFRSF6B antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection.)

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TNFRSF6B/DCR3, Polyclonal Antibody (Cat# AAA21319)

Full Name

TNFRSF6B/DCR3 Rabbit anti-Human Polyclonal Antibody

Gene Names

TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in mouse skeletal muscle with rabbit polyclonal antibody at 1:40000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistchemistry) (Immunochemical staining of alpha smooth muscle actin in mouse heart with rabbit polyclonal antibody at 1:40000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in mouse uterus with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in human breast carcinoma (from 3 donors) with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistchemistry) (Immunochemical staining of alpha smooth muscle actin in human breast (from 3 donors) with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in human skeletal muscle with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in human heart with rabbit polyclonal antibody at 1:20000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in rat small intestine with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of alpha smooth muscle actin in mouse small intestine with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).)

WB (Western Blot) (Anti-ACTA2 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: HEK293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa)

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ACTA2, Polyclonal Antibody (Cat# AAA27714)

Full Name

Anti-ACTA2 Antibody, Rabbit Polyclonal

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$185/0.02 mL $375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using FDX1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using FDX1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using FDX1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using FDX1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using FDX1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FDX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

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FDX1, Polyclonal Antibody (Cat# AAA28226)

Full Name

FDX1 Polyclonal Antibody

Gene Names

FDX1; ADX; FDX; LOH11CR1D

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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PD-ECGF, Polyclonal Antibody (Cat# AAA29250)

Full Name

PD-ECGF Polyclonal Antibody

Gene Names

TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (SDHA Antibody-Immunohistochemistry of paraffin-embedded rat heart.)

IHC (Immunohistchemistry) (SDHA Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer using SDHA antibody at dilution of 1:100 (400x lens).)

IHC (Immunohistochemistry) (SDHA Antibody-Immunohistochemistry of paraffin-embedded rat heart.)

IHC (Immunohistochemistry) (SDHA Antibody-Immunohistochemistry of paraffin-embedded human esophageal cancer tissue.)

IHC (Immunohistochemistry) (SDHA Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue.)

IHC (Immunohistochemistry) (SDHA Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (SDHA Antibody-Western blot analysis of extracts of various cell lines, using SDHA antibody.)

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SDHA, Polyclonal Antibody (Cat# AAA21315)

Full Name

SDHA Rabbit anti-Human Polyclonal Antibody

Gene Names

SDHA; FP; PGL5; SDH1; SDH2; SDHF; CMD1GG

Reactivity

Mouse, Rat, Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

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WB (Western Blot) (Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilution Lane A: TRIM28 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:89 kDa Observed band size:115 kDa (We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation) (TRIM28 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-TRIM28 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TRIM28 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 89 kDaObserved band size: 115 kDa)

WB (Western Blot) (Anti-TRIM28 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:89 kDaObserved band size:115 kDa(We are unsure as to the identity of these extra bands.))

IHC (Immunohistochemistry) (Immunochemical staining of human TRIM28 in human breast with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of human TRIM28 in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of human TRIM28 in human spleen with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

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KAP1/TRIM28, Polyclonal Antibody (Cat# AAA27715)

Full Name

Anti-KAP1/TRIM28 Antibody, Rabbit Polyclonal

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SCN3B antibody (AAA28227) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

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SCN3B, Polyclonal Antibody (Cat# AAA28227)

Full Name

SCN3B Polyclonal Antibody

Gene Names

SCN3B; SCNB3; ATFB16; BRGDA7; HSA243396

Reactivity

Human, Mouse

Applications

Western Blot (WB)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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MSH2, Polyclonal Antibody (Cat# AAA31299)

Full Name

Phospho-MSH2 (Tyr238) Antibody

Gene Names

MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using ifitm3 Polyclonal Antibody at dilution of 1:100)

IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using IFITM3 Polyclonal Antibody at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using IFITM3 Polyclonal Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse lung using IFITM3 Polyclonal Antibody at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using IFITM3 Polyclonal Antibody at dilution of 1:100)

WB (Western Blot) (Western blot with IFITM3 Polyclonal antibody at dilution of 1:2000.lane 1:Hela whole cell lysate, lane 2:293 T whole cell lysate, lane 3:Mouse Liver, lane 4:Mouse Heart,lane 5:Rat Liver)

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IFITM3, Polyclonal Antibody (Cat# AAA22340)

Full Name

IFITM3 Polyclonal Antibody

Gene Names

Ifitm3; rat8; DSPA2b

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antigen Affinity Purification

Pricing

$200/0.025 mL $350/0.1 mL $670/2x0.1 mL $970/3x0.1 mL $1,255/4x0.1 mL $1,510/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

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c-Myc, Polyclonal Antibody (Cat# AAA28997)

Full Name

c-Myc Polyclonal Antibody

Gene Names

MYC; MRTL; c-Myc; bHLHe39

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:400(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

WB (Western Blot) (Western blot analysis of CACO2 lysate, antibody was diluted at 1000. Secondary antibody)

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TIE2, Polyclonal Antibody (Cat# AAA30789)

Full Name

TIE2 Antibody

Gene Names

TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$305/0.05 mL $385/0.1 mL $740/2x0.1 mL $1,070/3x0.1 mL $1,385/4x0.1 mL $1,475/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using CK-18 Polyclonal Antibody at dilution of 1:300)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human cervical carcinoma using CK-18 Polyclonal Antibody at dilution of 1:300)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using CK-18 Polyclonal Antibody at dilution of 1:300)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using CK-18 Polyclonal Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer using CK-18 Polyclonal Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using CK-18 Polyclonal Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot CK-18 with Polyclonal Antibody at dilution of 1:1000..lane 1:HepG2,lane 2:Hela, lane 3:A549,lane 4:k562)

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CK-18, Polyclonal Antibody (Cat# AAA22342)

Full Name

CK-18 Polyclonal Antibody

Gene Names

Krt18; K18; CK18; Krt1-18

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen Affinity Purification

Pricing

$200/0.025 mL $350/0.1 mL $670/2x0.1 mL $970/3x0.1 mL $1,255/4x0.1 mL $1,510/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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ERK1/2, Polyclonal Antibody (Cat# AAA31300)

Full Name

Phospho-ERK1/2 (Thr177/Thr160) Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using HNRNPA2B1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HNRNPA2B1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-431 cells using HNRNPA2B1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] HNRNPA2B1 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] HNRNPA2B1 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] HNRNPA2B1 Polyclonal Antibody at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using HNRNPA2B1 Polyclonal Antibody at 1:500 dilution.)

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HNRNPA2B1, Polyclonal Antibody (Cat# AAA22330)

Full Name

HNRNPA2B1 Polyclonal Antibody

Gene Names

HNRNPA2B1; RNPA2; HNRPA2; HNRPB1; SNRPB1; HNRNPA2; HNRNPB1; HNRPA2B1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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CD148, Polyclonal Antibody (Cat# AAA29242)

Full Name

CD148 Polyclonal Antibody

Gene Names

PTPRJ; DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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Transfection Control (Anti-TPRA1 / GPR175 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Transfection (Anti-TPRA1 / GPR175 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with TPRA1 / GPR175.)

IHC (Immunohistchemistry) (Anti-TPRA1 / GPR175 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-TPRA1 / GPR175 antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-TPRA1 / GPR175 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-TPRA1 / GPR175 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-TPRA1 / GPR175 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-TPRA1 / GPR175 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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TPRA1 / GPR175, Polyclonal Antibody (Cat# AAA12365)

Full Name

Rabbit Polyclonal to Human TPRA1 / GPR175

Gene Names

TPRA1; GPR175; TPRA40; TMEM227

Reactivity

Gibbon, Gorilla, Human, Monkey
Predicted Reactivity: Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

$650/0.05 mg $1,255/2x0.05 mg $1,825/3x0.05 mg $2,365/4x0.05 mg $2,680/5x0.05 mg

WB (Western Blot) (Western BlotPositive WB detected in Recombinantprotein (80ng, 40ng, 20ng, 10ng)All lanes: Human IgG Fc antibody;HRPconjugated at 1:1000Predicted band size: 35 kDaObserved band size: 35 kDa)

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IgG Fc fragment, Polyclonal Secondary Antibody (Cat# AAA15949)

Full Name

Rabbit anti-Human IgG Fc fragment polyclonal Antibody, HRP conjugated

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB)

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$240/0.5 mg $455/2x0.5 mg $660/3x0.5 mg $850/4x0.5 mg $1,020/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TP53 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using TP53 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using TP53 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TP53 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)

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TP53, Polyclonal Antibody (Cat# AAA28237)

Full Name

TP53 Polyclonal Antibody

Gene Names

Trp53; bbl; bfy; bhy; p44; p53; Tp53

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FoxO3a antibody at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using FOXO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using FOXO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using FOXO3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver damage using FoxO3a antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using FoxO3a antibody at dilution of 1:100 .)

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FoxO3a, Polyclonal Antibody (Cat# AAA30541)

Full Name

FoxO3a Polyclonal Antibody

Gene Names

Foxo3; Fkhr2; C76856; FKHRL1; Foxo3a; 1110048B16Rik; 2010203A17Rik

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Anti-CD3 epsilon Picoband antibody, pb9093-7.JPGIHC(F): Mouse Spleen Tissue)

IHC (Immunohistchemistry) (Anti-CD3 epsilon Picoband antibody, pb9093-6.JPGIHC(F): Rat Spleen Tissue)

IHC (Immunohistochemistry) (Anti-CD3 epsilon Picoband antibody, AAA11598-5.JPGIHC(P): Rat Spleen Tissue)

IHC (Immunohistochemistry) (Anti-CD3 epsilon Picoband antibody, AAA11598-4.JPGIHC(P): Mouse Spleen Tissue)

IHC (Immunohistochemistry) (Anti-CD3 epsilon Picoband antibody, AAA11598-3.JPGIHC(P): Human Tonsil Tissue)

Application Data (Anti-CD3 epsilon Picoband antibody, AAA11598-2.jpgAll lanes: Anti CD3 Epsilon (AAA11598) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: CEM Whole Cell Lysate at 40ugPredicted bind size: 23KDObserved bind size: 23KD)

Application Data (Anti-CD3 epsilon Picoband antibody, AAA11598-1.jpgAll lanes: Anti CD3 Epsilon (AAA11598) at 0.5ug/mlWB : Recombinant Human CD3epsilon Protein 0.5ngPredicted bind size: 40KDObserved bind size: 40KD)

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CD3 epsilon, Polyclonal Antibody (Cat# AAA11598)

Full Name

Anti-CD3 epsilon Antibody

Gene Names

CD3E; T3E; TCRE; IMD18

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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Transfection Control (HEK293 cells transfected with empty vector)

Transfection (HEK293 cells expressing human XPR1)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 8 ug/ml.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 8 ug/ml.)

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XPR1, Polyclonal Antibody (Cat# AAA12366)

Full Name

Rabbit Polyclonal to Human XPR1

Gene Names

XPR1; X3; SYG1

Reactivity

Bat, Gibbon, Bovine, Chicken, Dog, Hamster, Horse, Human, Monkey, Mouse, Orangutan, Pig, Rabbit, Rat
Predicted Reactivity: Xenopus, Zebrafish, Insect (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC)

Purity

Immunoaffinity Purified

Pricing

$630/0.05 mg $1,215/2x0.05 mg $1,770/3x0.05 mg $2,295/4x0.05 mg $2,575/5x0.05 mg

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-PCCA antibody (AAA19790).Overlay histogram showing HepG2 cells stained with AAA19790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCCA Antibody (AAA19790, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of 293T cells using anti-PCCA antibody (AAA19790).Overlay histogram showing 293T cells stained with AAA19790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCCA Antibody (AAA19790, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PCCA using anti-PCCA antibody (AAA19790).PCCA was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCCA Antibody (AAA19790) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat liver tissue lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCCA antigen affinity purified polyclonal antibody (#AAA19790) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PCCA at approximately 70 kDa. The expected band size for PCCA is at 80 kDa.)

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PCCA, Polyclonal Antibody (Cat# AAA19790)

Full Name

Anti-PCCA Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.