Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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DR4, Polyclonal Antibody (Cat# AAA10950)

Full Name

DR4 Antibody

Gene Names

TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1

Reactivity

Human

Applications

EIA, WB

Purity

DR4 Antibody is affinity chromatography purified via peptide column.

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Abi 1, Polyclonal Antibody (Cat# AAA31442)

Full Name

Phospho-Abi 1 (Tyr213) Antibody

Gene Names

ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

WB, IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-RAB3IP antibody (AAA19919).Overlay histogram showing MCF-7 cells stained with AAA19919 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB3IP Antibody (AAA19919, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RAB3IP using anti-RAB3IP antibody (AAA19919).RAB3IP was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB3IP Antibody (AAA19919) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB3IP Antibody (AAA19919) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB3IP Antibody (AAA19919) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RAB3IP Antibody (AAA19919) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB3IP antigen affinity purified polyclonal antibody (#AAA19919) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB3IP at approximately 55 kDa. The expected band size for RAB3IP is at 53 kDa.)

RAB3IP, Polyclonal Antibody (Cat# AAA19919)

Full Name

Anti-RAB3IP Antibody Picoband

Gene Names

RAB3IP; RABIN3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-IARS2 antibody (AAA19929).Overlay histogram showing 293T cells stained with AAA19929 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IARS2 Antibody (AAA19929, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of IARS2 using anti-IARS2 antibody (AAA19929).IARS2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IARS2 Antibody (AAA19929) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IARS2 Antibody (AAA19929) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IARS2 Antibody (AAA19929) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IARS2 Antibody (AAA19929) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-IARS2 Antibody (AAA19929) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human Hacat whole cell lysates,Lane 6: human HepG2 whole cell lysates,Lane 7: rat brain tissue lysates,Lane 8: rat kidney tissue lysates,Lane 9: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IARS2 antigen affinity purified polyclonal antibody (#AAA19929) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IARS2 at approximately 114 kDa. The expected band size for IARS2 is at 114 kDa.)

IARS2, Polyclonal Antibody (Cat# AAA19929)

Full Name

Anti-IARS2 Antibody Picoband

Gene Names

IARS2; ILERS

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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Application Data (C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal cells and the subpopulation of endothelial cells. Virtually all other dispersed apparent mesenchymal cells express FGFR1 and FGFR2 (merged image in E). F: FGFR2 (F) and FGFR1 (G) staining in clustered cells of epithelial origin (inferred by morphology here) demonstrating that epithelial cells express both FGFR1 and FGFR2 (merged image with DAPI staining in H).)

FGFR2, Polyclonal Antibody (Cat# AAA26851)

Full Name

FGFR2, NT (FGFR2, BEK, KGFR, KSAM, Fibroblast growth factor receptor 2, K-sam, Keratinocyte growth factor receptor, CD332) (AP)

Gene Names

FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM

Reactivity

Human, Monkey, Mouse, Rat

Applications

IF, EIA, IHC, WB

Purity

Purified by Protein G Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (ICC staining SHP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

SHP-1, Polyclonal Antibody (Cat# AAA29937)

Full Name

SHP-1 Antibody

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC

Purity

Peptide affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-Nedd4 antibody (AAA19414).Overlay histogram showing HEPA1-6 cells stained with AAA19414 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nedd4 Antibody (AAA19414, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Nedd4, Polyclonal Antibody (Cat# AAA19414)

Full Name

Anti-Nedd4 Antibody Picoband

Gene Names

Nedd4; Nedd4a; Nedd4-1; AA959633; AL023035; AU019897; mKIAA0093; E430025J12Rik

Reactivity

Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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TAF4, Polyclonal Antibody (Cat# AAA19307)

Full Name

Anti-TAF4 Antibody

Gene Names

TAF4; TAF2C; TAF4A; TAF2C1; TAFII130; TAFII135

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue)

Hepatoma Derived Growth Factor, Polyclonal Antibody (Cat# AAA20850)

Full Name

Polyclonal Antibody to Hepatoma Derived Growth Factor (HDGF)

Gene Names

HDGF; HMG1L2

Reactivity

Human

Applications

WB, ICC, IHC, EIA

Purity

Affinity Chromatography

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-OTULIN antibody (AAA19319).Overlay histogram showing 293T cells stained with AAA19319 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OTULIN Antibody (AAA19319, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

OTULIN, Polyclonal Antibody (Cat# AAA19319)

Full Name

Anti-OTULIN Antibody

Gene Names

OTULIN; GUM; FAM105B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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AHA1/AHSA1, Polyclonal Antibody (Cat# AAA19557)

Full Name

Anti-AHA1/AHSA1 Antibody Picoband

Gene Names

AHSA1; p38; AHA1; C14orf3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U251 cells using anti-MSF/SEPTIN9 antibody (AAA19540).Overlay histogram showing U251 cells stained with AAA19540 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSF/SEPTIN9 Antibody (AAA19540, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MSF/SEPTIN9, Polyclonal Antibody (Cat# AAA19540)

Full Name

Anti-MSF/SEPTIN9 Antibody Picoband

Gene Names

SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25

Reactivity

Human, Mouse

Applications

WB, IHC, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of CPI17 alpha using anti- CPI17 alpha antibody (AAA19176).CPI17 alpha was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CPI17 alpha Antibody (AAA19176) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

CPI17 alpha, Polyclonal Antibody (Cat# AAA19176)

Full Name

Anti-CPI17 alpha Picoband Antibody

Gene Names

PPP1R14A; CPI17; CPI-17; PPP1INL

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-Hamartin/TSC1 antibody (AAA19734).Overlay histogram showing THP-1 cells stained with AAA19734 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hamartin/TSC1 Antibody (AAA19734, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (AAA19734).Hamartin/TSC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Hamartin/TSC1 Antibody (AAA19734) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin/TSC1 antigen affinity purified polyclonal antibody (#AAA19734) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Hamartin/TSC1 at approximately 160 kDa. The expected band size for Hamartin/TSC1 is at 130 kDa.)

Hamartin/TSC1, Polyclonal Antibody (Cat# AAA19734)

Full Name

Anti-Hamartin/TSC1 Antibody Picoband

Gene Names

TSC1; LAM; TSC

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

PERK, Polyclonal Antibody (Cat# AAA28957)

Full Name

PERK (phospho Thr981) Polyclonal Antibody

Gene Names

EIF2AK3; PEK; WRS; PERK

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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EPAS-1, Polyclonal Antibody (Cat# AAA30775)

Full Name

EPAS-1 Rabbit Polyclonal Antibody

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human, Mouse, Rat

Applications

IF, ICC, WB, IHC-P, EIA

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in rat liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

HMGB1, Polyclonal Antibody (Cat# AAA27719)

Full Name

Anti-HMGB1 Antibody, Rabbit Polyclonal

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, IP

Purity

Protein A & Antigen Affinity

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BAX antibody.)

BAX, Polyclonal Antibody (Cat# AAA29799)

Full Name

BAX Polyclonal Antibody

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat

Applications

WB, IF

Purity

Affinity purification

Pricing

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C-Kit, Polyclonal Antibody (Cat# AAA31085)

Full Name

C-Kit Antibody

Gene Names

KIT; PBT; SCFR; C-Kit; CD117; MASTC

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using HMGN1 antibody at dilution of 1:100 (40x lens).)

HMGN1, Polyclonal Antibody (Cat# AAA28278)

Full Name

HMGN1 Polyclonal Antibody

Gene Names

HMGN1; HMG14

Reactivity

Mouse, Rat

Applications

IHC

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IL-13Ralpha2, Polyclonal Antibody (Cat# AAA29309)

Full Name

IL-13Ralpha2 Polyclonal Antibody

Gene Names

IL13RA2; CT19; IL-13R; IL13BP; CD213A2

Reactivity

Human

Applications

WB, IHC-P

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

JAK1, Polyclonal Antibody (Cat# AAA29052)

Full Name

JAK1 Polyclonal Antibody

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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ICC (Immunocytochemistry) (Anti- TLR7 Picoband antibody, AAA11659, ICCICC: A549 Cell)

TLR7, Polyclonal Antibody (Cat# AAA11659)

Full Name

Anti-TLR7 Antibody

Gene Names

TLR7; TLR7-like

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC

Purity

Immunogen Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of PRDM1/Blimp1 using anti-PRDM1/Blimp1 antibody (AAA19133).PRDM1/Blimp1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRDM1/Blimp1 Antibody (AAA19133) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

PRDM1/Blimp1, Polyclonal Antibody (Cat# AAA19133)

Full Name

Anti-PRDM1/Blimp1 Picoband antibody

Gene Names

PRDM1; BLIMP1; PRDI-BF1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

EIA, IHC, WB

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using BCAP29 antibody at dilution of 1:100 (40x lens).)

BCAP29, Polyclonal Antibody (Cat# AAA28093)

Full Name

BCAP29 Polyclonal Antibody

Gene Names

BCAP29; BAP29

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity Purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CTNNA1 antibody. Western blot was performed from the immunoprecipitate using CTNNA1 antibody at a dilition of 1:500.)

CTNNA1, Polyclonal Antibody (Cat# AAA28098)

Full Name

CTNNA1 Polyclonal Antibody

Gene Names

CTNNA1; CAP102

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, FC/FACS

Purity

Affinity Purification

Pricing

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PUMA, Polyclonal Antibody (Cat# AAA10943)

Full Name

PUMA Antibody

Gene Names

BBC3; JFY1; PUMA; JFY-1

Reactivity

Human, Mouse

Applications

EIA, WB, ICC, IF

Purity

PUMA Antibody is affinity chromatography purified via peptide column.

Pricing

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DB (Dot Blot) (Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17797)

Full Name

Amyloid Fibrils (OC) Antibody: FITC

Reactivity

Human. Potentially mouse and rat based on species homology.

Applications

IP, ICC, IHC, EIA, WB, DB

Pricing

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SRF, Polyclonal Antibody (Cat# AAA31435)

Full Name

Phospho-SRF (Thr159) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Cyclin E1, Polyclonal Antibody (Cat# AAA31439)

Full Name

Phospho-Cyclin E1 (Ser399) Antibody

Gene Names

CCNE1; CCNE

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (88%), Horse (88%), Sheep (88%), Chicken (88%), Xenopus (88%)

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Figure 7. IF analysis of OSGIN1 using anti-OSGIN1 antibody (AAA19937).OSGIN1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 6. IF analysis of OSGIN1 and Tubulin beta using anti-OSGIN1 antibody (AAA19937) and anti-Tubulin beta antibody (M05613-4).OSGIN1 and Tubulin beta was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-OSGIN1 Antibody (AAA19937) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human T-47D whole cell lysates,Lane 3: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSGIN1 antigen affinity purified polyclonal antibody (#AAA19937) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for OSGIN1 at approximately 53 kDa. The expected band size for OSGIN1 is at 61 kDa.)

OSGIN1, Polyclonal Antibody (Cat# AAA19937)

Full Name

Anti-OSGIN1 Antibody Picoband

Gene Names

OSGIN1; BDGI; OKL38

Reactivity

Human

Applications

WB, IHC, ICC, IF, EIA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Monoamine Oxidase B (Monoamine Oxidase B (MAOB)) Rabbit pAb.)

MAOB, Polyclonal Antibody (Cat# AAA29672)

Full Name

MAOB Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using JMJD6 Antibody at dilution of 1:100 (40x lens).)

JMJD6, Polyclonal Antibody (Cat# AAA28145)

Full Name

JMJD6 Polyclonal Antibody

Gene Names

JMJD6; PSR; PTDSR; PTDSR1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HeLa cells using anti-NDUFS5 antibody (AAA19505).Overlay histogram showing HeLa cells stained with AAA19505 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS5 Antibody (AAA19505, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NDUFS5, Polyclonal Antibody (Cat# AAA19505)

Full Name

Anti-NDUFS5 Antibody Picoband

Gene Names

NDUFS5; CI15K; CI-15k

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, WB

Purity

Immunogen affinity purified.

Pricing

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MSK2/RSK-B/RPS6KA4, Polyclonal Antibody (Cat# AAA19553)

Full Name

Anti-MSK2/RSK-B/RPS6KA4 Antibody Picoband

Gene Names

RPS6KA4; MSK2; RSK-B

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

CD85g, Polyclonal Antibody (Cat# AAA29200)

Full Name

CD85g Polyclonal Antibody

Gene Names

LILRA4; ILT7; CD85g

Reactivity

Human

Applications

WB, IHC-P

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat spleen using THBS1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

THBS1, Polyclonal Antibody (Cat# AAA22313)

Full Name

THBS1 Polyclonal Antibody

Gene Names

THBS1; TSP; THBS; TSP1; TSP-1; THBS-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot of TLR3 in Daudi cell lysate with TLR3 antibody at (A) 1 and (B) 2 ug/ml.)

TLR3, Polyclonal Antibody (Cat# AAA12329)

Full Name

Rabbit Polyclonal to Human TLR3

Gene Names

TLR3; CD283; IIAE2

Reactivity

Human, Mouse

Applications

IHC - Paraffin, ICC, WB, EIA

Purity

Ion exchange Chromatography

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

CD284, Polyclonal Antibody (Cat# AAA28982)

Full Name

CD284 Polyclonal Antibody

Gene Names

TLR4; TOLL; CD284; TLR-4; ARMD10

Reactivity

Human, Mouse

Applications

WB, IHC-P, IF

Pricing

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IP (Immunoprecipitation) (HDAC2 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg NIH-3T3 Whole Cell LysateLane C:0.5 mg Hela Whole Cell Lysate4 uL anti-HDAC2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HDAC2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 60 kDa)

HDAC2, Polyclonal Antibody (Cat# AAA27718)

Full Name

Anti-HDAC2 Antibody, Rabbit Polyclonal

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, IP

Purity

Protein A & Antigen Affinity

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DDX5 antibody. Blue: DAPI for nuclear staining.)

DDX5, Polyclonal Antibody (Cat# AAA28263)

Full Name

DDX5 Polyclonal Antibody

Gene Names

DDX5; p68; HLR1; G17P1; HUMP68

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HepG2 cells using anti-Vaspin/SERPINA12 antibody (AAA19840).Overlay histogram showing HepG2 cells stained with AAA19840 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Vaspin/SERPINA12 using anti-Vaspin/SERPINA12 antibody (AAA19840).Vaspin/SERPINA12 was detected in a paraffin-embedded section of adipose of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Vaspin/SERPINA12 Antibody (AAA19840) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse B16-F10 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vaspin/SERPINA12 antigen affinity purified polyclonal antibody (#AAA19840) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Vaspin/SERPINA12 at approximately 55 kDa. The expected band size for Vaspin/SERPINA12 is at 47 kDa.)

Vaspin/SERPINA12, Polyclonal Antibody (Cat# AAA19840)

Full Name

Anti-Vaspin/SERPINA12 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

Cleaved-Caspase-8, Polyclonal Antibody (Cat# AAA28833)

Full Name

Cleaved-Caspase-8 (D384) Polyclonal Antibody

Gene Names

CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8

Reactivity

Human

Applications

WB, IHC-P, IF

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300)

Cleaved-Caspase-3 p17, Polyclonal Antibody (Cat# AAA28830)

Full Name

Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Tubulin alpha, Polyclonal Antibody (Cat# AAA28850)

Full Name

Tubulin alpha (Acetyl Lys40) Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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ZNF433, Polyclonal Antibody (Cat# AAA29815)

Full Name

ZNF433 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

Purity

Affinity purification

Pricing

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Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17801)

Full Name

Amyloid Fibrils (OC) Antibody: ATTO 594

Reactivity

Human. Potentially mouse and rat based on species homology.

Applications

IP, ICC, IHC, EIA, WB, DB

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AMPKalpha1 at 1:1000 dilution.)

AMPKalpha1, Polyclonal Antibody (Cat# AAA30645)

Full Name

AMPKalpha1 Rabbit Polyclonal Antibody

Gene Names

PRKAA1; AMPK; AMPKa1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Synapsin I, Polyclonal Antibody (Cat# AAA29127)

Full Name

Synapsin I Polyclonal Antibody

Gene Names

SYN1; SYNI; SYN1a; SYN1b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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GNAS, Polyclonal Antibody (Cat# AAA23504)

Full Name

GNAS antibody - N-terminal region

Gene Names

GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45

Reactivity

Cow, Human, Mouse, Pig, Rabbit, Rat

Applications

IHC, WB

Purity

Protein A purified

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Polyclonal Antibodies

Viewing 1300-1350 of 3176 product results

DR4, Polyclonal Antibody (Cat# AAA10950)

Abi 1, Polyclonal Antibody (Cat# AAA31442)

RAB3IP, Polyclonal Antibody (Cat# AAA19919)

IARS2, Polyclonal Antibody (Cat# AAA19929)

FGFR2, Polyclonal Antibody (Cat# AAA26851)

SHP-1, Polyclonal Antibody (Cat# AAA29937)

Nedd4, Polyclonal Antibody (Cat# AAA19414)

TAF4, Polyclonal Antibody (Cat# AAA19307)

Hepatoma Derived Growth Factor, Polyclonal Antibody (Cat# AAA20850)

OTULIN, Polyclonal Antibody (Cat# AAA19319)

AHA1/AHSA1, Polyclonal Antibody (Cat# AAA19557)

MSF/SEPTIN9, Polyclonal Antibody (Cat# AAA19540)

CPI17 alpha, Polyclonal Antibody (Cat# AAA19176)

Hamartin/TSC1, Polyclonal Antibody (Cat# AAA19734)

PERK, Polyclonal Antibody (Cat# AAA28957)

EPAS-1, Polyclonal Antibody (Cat# AAA30775)

HMGB1, Polyclonal Antibody (Cat# AAA27719)

BAX, Polyclonal Antibody (Cat# AAA29799)

C-Kit, Polyclonal Antibody (Cat# AAA31085)

HMGN1, Polyclonal Antibody (Cat# AAA28278)

IL-13Ralpha2, Polyclonal Antibody (Cat# AAA29309)

JAK1, Polyclonal Antibody (Cat# AAA29052)

TLR7, Polyclonal Antibody (Cat# AAA11659)

PRDM1/Blimp1, Polyclonal Antibody (Cat# AAA19133)

BCAP29, Polyclonal Antibody (Cat# AAA28093)

CTNNA1, Polyclonal Antibody (Cat# AAA28098)

PUMA, Polyclonal Antibody (Cat# AAA10943)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17797)

SRF, Polyclonal Antibody (Cat# AAA31435)

Cyclin E1, Polyclonal Antibody (Cat# AAA31439)

OSGIN1, Polyclonal Antibody (Cat# AAA19937)

MAOB, Polyclonal Antibody (Cat# AAA29672)

JMJD6, Polyclonal Antibody (Cat# AAA28145)

NDUFS5, Polyclonal Antibody (Cat# AAA19505)

MSK2/RSK-B/RPS6KA4, Polyclonal Antibody (Cat# AAA19553)

CD85g, Polyclonal Antibody (Cat# AAA29200)

THBS1, Polyclonal Antibody (Cat# AAA22313)

TLR3, Polyclonal Antibody (Cat# AAA12329)

CD284, Polyclonal Antibody (Cat# AAA28982)

HDAC2, Polyclonal Antibody (Cat# AAA27718)

DDX5, Polyclonal Antibody (Cat# AAA28263)

Vaspin/SERPINA12, Polyclonal Antibody (Cat# AAA19840)

Cleaved-Caspase-8, Polyclonal Antibody (Cat# AAA28833)

Cleaved-Caspase-3 p17, Polyclonal Antibody (Cat# AAA28830)

Tubulin alpha, Polyclonal Antibody (Cat# AAA28850)

ZNF433, Polyclonal Antibody (Cat# AAA29815)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17801)

AMPKalpha1, Polyclonal Antibody (Cat# AAA30645)

Synapsin I, Polyclonal Antibody (Cat# AAA29127)

GNAS, Polyclonal Antibody (Cat# AAA23504)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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