Polyclonal Antibodies

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U87 cells using anti-Symplekin/SYMPK antibody (AAA19851).Overlay histogram showing U87 cells stained with AAA19851 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Symplekin/SYMPK Antibody (AAA19851, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of Symplekin/SYMPK using anti-Symplekin/SYMPK antibody (AAA19851) and anti-Tubulin Alpha antibody (M03989-3).Symplekin/SYMPK was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Symplekin/SYMPK Antibody (AAA19851) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human 293T whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: rat testis tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Symplekin/SYMPK antigen affinity purified polyclonal antibody (#AAA19851) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Symplekin/SYMPK at approximately 150 kDa. The expected band size for Symplekin/SYMPK is at 141 kDa.)

Symplekin/SYMPK, Polyclonal Antibody (Cat# AAA19851)

Full Name

Anti-Symplekin/SYMPK Antibody Picoband

Gene Names

SYMPK; SPK; SYM; FLJ27092

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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LAS1L, Polyclonal Antibody (Cat# AAA23435)

Full Name

LAS1L Antibody - middle region

Gene Names

LAS1L; WTS; Las1-like; dJ475B7.2

Reactivity

Cow, Horse, Human

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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COPB2, Polyclonal Antibody (Cat# AAA29835)

Full Name

COPB2 Polyclonal Antibody

Gene Names

COPB2; MCPH19; beta'-COP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of DDB1 using anti-DDB1 antibody (AAA11660).DDB1 was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DDB1 Antibody (AAA11660) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

DDB1, Polyclonal Antibody (Cat# AAA11660)

Full Name

Anti-DDB1 Antibody

Gene Names

DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen Affinity Purified

Pricing

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DB (Dot Blot) (Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)

Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17804)

Full Name

Amyloid Fibrils (OC) Antibody

Reactivity

Human. Potentially mouse and rat based on species homology.

Applications

Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB), Dot Blot (DB)

Pricing

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WB (Western Blot) (WB Suggested Anti-KCNN4 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate)

KCNN4, Polyclonal Antibody (Cat# AAA23440)

Full Name

KCNN4 antibody - C-terminal region

Gene Names

KCNN4; IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1

Reactivity

Cow, Horse, Human, Pig, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry-Paraffin) (Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min; Incubation: Anti-eIF4A3 Polyclonal Antibody, Unconjugated(bs-14548R) 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

eIF4A3, Polyclonal Antibody (Cat# AAA28816)

Full Name

eIF4A3 Polyclonal Antibody

Gene Names

EIF4A3; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII

Reactivity

Rat, Dog, Cow

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)

PTEN, Polyclonal Antibody (Cat# AAA29328)

Full Name

PTEN Polyclonal Antibody

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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LC3B, Polyclonal Antibody (Cat# AAA29840)

Full Name

LC3B Polyclonal Antibody

Gene Names

MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (Dilution: IF: 1:50-200 WB 1:500-2000, IHC 1:50-300, ELISA 1:10000-20000)

Caspase-1, Polyclonal Antibody (Cat# AAA29325)

Full Name

Caspase-1 Polyclonal Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GABARAP at 1:1000 dilution.)

GABARAP, Polyclonal Antibody (Cat# AAA30605)

Full Name

GABARAP Rabbit Polyclonal Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

GIT2, Polyclonal Antibody (Cat# AAA31373)

Full Name

Phospho-GIT2 (Tyr592) Antibody

Gene Names

GIT2; CAT2; CAT-2

Reactivity

Human
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U937 cells using anti-GRIM19/NDUFA13 antibody (AAA19857).Overlay histogram showing U937 cells stained with AAA19857 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIM19/NDUFA13 Antibody (AAA19857, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of GRIM19/NDUFA13 using anti-GRIM19/NDUFA13 antibody (AAA19857).GRIM19/NDUFA13 was detected in a paraffin-embedded section of human urothelial carcinoma with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GRIM19/NDUFA13 Antibody (AAA19857) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GRIM19/NDUFA13 Antibody (AAA19857) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GRIM19/NDUFA13 Antibody (AAA19857) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat skeletal muscle tissue lysates,Lane 3: mouse heart tissue lysates,Lane 4: mouse skeletal muscle tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIM19/NDUFA13 antigen affinity purified polyclonal antibody (#AAA19857) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (17 kDa. The expected band size for GRIM19/NDUFA13 is at 16-18 kDa.)

GRIM19/NDUFA13, Polyclonal Antibody (Cat# AAA19857)

Full Name

Anti-GRIM19/NDUFA13 Antibody Picoband

Gene Names

NDUFA13; B16.6; CDA016; CGI-39; GRIM19; GRIM-19

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

VPX, Polyclonal Antibody (Cat# AAA27022)

Full Name

VPX Antibody

Reactivity

Human immunodeficiency virus type 2 subtype A

Applications

ELISA (EIA), Western Blot (WB)

Purity

>95%, Protein G purified

Pricing

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PEA15-S104, Polyclonal Antibody (Cat# AAA28302)

Full Name

Phospho-PEA15-S104 pAb

Gene Names

PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Sample: HL-60 Cell (Human) Lysate at 30 ug Primary: Anti-P2Y9 (bs-12074R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD)

P2Y9, Polyclonal Antibody (Cat# AAA28814)

Full Name

P2Y9 Polyclonal Antibody

Gene Names

Lpar4; LPA4; p2y9; Gpr23; 5730485F04Rik

Reactivity

Rat, Pig, Dog, Cow

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

MMP-13, Polyclonal Antibody (Cat# AAA29070)

Full Name

MMP-13 Polyclonal Antibody

Gene Names

MMP13; CLG3; MANDP1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

gp120 (Clade B), Polyclonal Antibody (Cat# AAA13711)

Full Name

Anti-gp120 (Clade B)

Applications

Western Blot (WB), ELISA (EIA)

Purity

Immunoaffinity chromatography.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry-PDCD10 Polyclonal Antibody)

PDCD10, Polyclonal Antibody (Cat# AAA28300)

Full Name

PDCD10 Polyclonal Antibody

Gene Names

PDCD10; CCM3; TFAR15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

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YAP1, Polyclonal Antibody (Cat# AAA10637)

Full Name

YAP1 Polyclonal Antibody

Gene Names

YAP1; YAP; YKI; COB1; YAP2; YAP65

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-POR antibody (AAA11661).Overlay histogram showing SiHa cells stained with AAA11661 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POR Antibody (AAA11661,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

POR, Polyclonal Antibody (Cat# AAA11661)

Full Name

Anti-POR Antibody

Gene Names

POR; CPR; CYPOR; P450R

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen Affinity Purified

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Melanopsin, Polyclonal Antibody (Cat# AAA29066)

Full Name

Melanopsin Polyclonal Antibody

Gene Names

OPN4; MOP

Reactivity

Human

Applications

Western Blot (WB), Immunofluorescence (IF)

Pricing

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ICC (Immunocytochemistry) (Anti- TLR7 Picoband antibody, AAA11659, ICCICC: A549 Cell)

TLR7, Polyclonal Antibody (Cat# AAA11659)

Full Name

Anti-TLR7 Antibody

Gene Names

TLR7; TLR7-like

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunocytochemistry (ICC)

Purity

Immunogen Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using KIAA1456 antibody.)

KIAA1456, Polyclonal Antibody (Cat# AAA29839)

Full Name

KIAA1456 Polyclonal Antibody

Gene Names

TRMT9B; TRM9L; hTRM9L; C8orf79; KIAA1456

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CBX5 at dilution of 1:100 (40x lens).)

CBX5, Polyclonal Antibody (Cat# AAA30607)

Full Name

CBX5 Rabbit Polyclonal Antibody

Gene Names

CBX5; HP1; HP1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of 293T cells using anti-MIA3 antibody (AAA19862).Overlay histogram showing 293T cells stained with AAA19862 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIA3 Antibody (AAA19862, 1ug/1x106 cells) for 30 min at 20 degree C. DyLightMIA3 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of MIA3 using anti-MIA3 antibody (AAA19862).MIA3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIA3 Antibody (AAA19862) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIA3 Antibody (AAA19862) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MIA3 Antibody (AAA19862) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: human Hacat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIA3 antigen affinity purified polyclonal antibody (#AAA19862) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MIA3 at approximately 300 kDa. The expected band size for MIA3 is at 300 kDa.)

MIA3, Polyclonal Antibody (Cat# AAA19862)

Full Name

Anti-MIA3 Antibody Picoband

Gene Names

MIA3; ARNT; D320; TANGO; TANGO1; UNQ6077

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000)

Perforin 1, Polyclonal Antibody (Cat# AAA29334)

Full Name

Perforin 1 Polyclonal Antibody

Gene Names

PRF1; P1; PFP; FLH2; PFN1; HPLH2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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HNRNPA1, Polyclonal Antibody (Cat# AAA29846)

Full Name

HNRNPA1 Polyclonal Antibody

Gene Names

HNRNPA1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

RhoA, Polyclonal Antibody (Cat# AAA31383)

Full Name

Phospho-RhoA (Ser188) Antibody

Gene Names

RHOA; ARHA; ARH12; RHO12; RHOH12

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (WB Suggested Anti-EED Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:2500Positive Control: OVCAR-3 cell lysateEED is strongly supported by BioGPS gene expression data to be expressed in Human OVCAR3 cells)

EED, Polyclonal Antibody (Cat# AAA23445)

Full Name

EED antibody - N-terminal region

Gene Names

EED; HEED; COGIS; WAIT1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Affinity Purified

Pricing

MMP26, Polyclonal Antibody (Cat# AAA27797)

Full Name

Anti-MMP26 Antibody

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB)

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

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IHC (Immunohistchemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (LCAT) Polyclonal Antibody, Unconjugated (bs-1972R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

LCAT, Polyclonal Antibody (Cat# AAA28821)

Full Name

LCAT Polyclonal Antibody

Reactivity

Rat, Pig, Dog, Cow

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Myogenin, Polyclonal Antibody (Cat# AAA29077)

Full Name

Myogenin Polyclonal Antibody

Gene Names

MYOG; MYF4; myf-4; bHLHc3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

Nanos Homologue 1, Polyclonal Antibody (Cat# AAA29333)

Full Name

Nanos Homologue 1 (NANOS1) Polyclonal Antibody

Gene Names

NANOS1; NOS1; SPGF12

Reactivity

Human

Applications

Western Blot (WB)

Pricing

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MAPK3, Polyclonal Antibody (Cat# AAA10646)

Full Name

MAPK3 Polyclonal Antibody

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using FOLH1 Polycloanl Antibody at dilution of 1:200)

FOLH1, Polyclonal Antibody (Cat# AAA22168)

Full Name

FOLH1 Polyclonal Antibody

Gene Names

Folh1; GCP2; mopsm

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC)

Purity

Antigen affinity purification

Pricing

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VEZF1, Polyclonal Antibody (Cat# AAA23448)

Full Name

VEZF1 antibody - middle region

Gene Names

VEZF1; DB1; ZNF161

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

TriMethyl-Histone H3-K64, Polyclonal Antibody (Cat# AAA28312)

Full Name

TriMethyl-Histone H3-K64 Rabbit pAb

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)

ALB, Polyclonal Antibody (Cat# AAA29336)

Full Name

ALB Polyclonal Antibody

Gene Names

ALB; PRO0883; PRO0903; PRO1341

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using ANXA8L2 antibody.)

ANXA8L2, Polyclonal Antibody (Cat# AAA29848)

Full Name

ANXA8L2 Polyclonal Antibody

Gene Names

ANXA8L1; ANXA8; ANXA8L2; VAC-beta; bA145E20.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using CSNK2A2 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

CSNK2A2, Polyclonal Antibody (Cat# AAA30616)

Full Name

CSNK2A2 Rabbit Polyclonal Antibody

Gene Names

CSNK2A2; CK2A2; CSNK2A1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of CRM1 using anti-CRM1 antibody (AAA11673).CRM1 was detected in frozen section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRM1 Antibody (AAA11673) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

CRM1, Polyclonal Antibody (Cat# AAA11673)

Full Name

Anti-CRM1 Antibody

Gene Names

XPO1; emb; CRM1; exp1

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

NA (A/Vietnam/1203/2004)(H5N1, human), Polyclonal Antibody (Cat# AAA13721)

Full Name

Anti-NA (A/Vietnam/1203/2004)(H5N1, human)

Applications

Western Blot (WB), ELISA (EIA)

Purity

Immunoaffinity chromatography

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using FGB Polycloanl Antibody at dilution of 1:200)

FGB, Polyclonal Antibody (Cat# AAA22170)

Full Name

FGB Polyclonal Antibody

Gene Names

Fgb; Ab1-181; Ab1-216; Ac1-581

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-MLXIP Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: PANC1 cell lysateMLXIP is supported by BioGPS gene expression data to be expressed in PANC1)

MLXIP, Polyclonal Antibody (Cat# AAA23450)

Full Name

MLXIP antibody - middle region

Gene Names

MLXIP; MIR; MONDOA; bHLHe36

Reactivity

Dog, Human, Mouse, Rabbit

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse colon using Galectin 3/Galectin 3/LGALS3 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)