Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (At 1/200 staining Human prostate cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/200 staining Rat intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of CDK4 (Phospho-Thr172) using serum treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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CDK4, Polyclonal Antibody (Cat# AAA31380)

Full Name

Phospho-CDK4 (Thr172) Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (85%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (92%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (Figure 10. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ICC (Immunocytochemistry) (Figure 7. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ICC (Immunocytochemistry) (Figure 6. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ICC (Immunocytochemistry) (Figure 5. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Lamin B1 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Lamin B1 Antibody (AAA11669) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (AAA11669).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: U87 Whole Cell Lysate,Lane 2: PC-12 Whole Cell Lysate,Lane 3: NIH3T3 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 67KD. The expected band size for Lamin B1 is at 67KD.)

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Lamin B1, Polyclonal Antibody (Cat# AAA11669)

Full Name

Anti-Lamin B1 Antibody

Gene Names

LMNB1; LMN; ADLD; LMN2; LMNB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen Affinity Purified

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-KCNJ12 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human brain)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal StomachAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal PancreasAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: KCNJ12Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-KCNJ12 antibodyFormalin Fixed Paraffin Embedded Tissue: Human Adult Skeletal muscleObserved Staining: Cytoplasm in hepatocytesPrimary Antibody Concentration: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

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KCNJ12, Polyclonal Antibody (Cat# AAA23443)

Full Name

KCNJ12 antibody - middle region

Gene Names

KCNJ12; IRK2; hIRK; IRK-2; hIRK1; KCNJN1; Kir2.2; Kir2.2v; kcnj12x; hkir2.2x

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using TBCB at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using TBCB at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using TBCB at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using TBCB at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using TBCB at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TBCB at 1:3000 dilution.)

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TBCB, Polyclonal Antibody (Cat# AAA30611)

Full Name

TBCB Rabbit Polyclonal Antibody

Gene Names

TBCB; CG22; CKAP1; CKAPI

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using FGB Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using FGB Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse liver using FGB Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human kidney using FGB Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using FGB Polycloanl Antibody at dilution of 1:200)

WB (Western Blot) (Western Blot analysis of HepG2, Rat heart, Rat liver and Mouse heart using FGB Polyclonal Antibody at dilution of 1:4000)

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FGB, Polyclonal Antibody (Cat# AAA22169)

Full Name

FGB Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $340/0.12 mL $485/0.2 mL $935/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $2,075/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, IHC-p 1:50-300, ELISA 1:10000-20000)

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NLRX1, Polyclonal Antibody (Cat# AAA29337)

Full Name

NLRX1 Polyclonal Antibody

Gene Names

NLRX1; NOD5; NOD9; NOD26; DLNB26; CLR11.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using SMAD2 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using SMAD2 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SMAD2 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using SMAD2 antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and SMAD2 knockout (KO) HeLa cells, using SMAD2 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SMAD2 antibody.)

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SMAD2, Polyclonal Antibody (Cat# AAA29849)

Full Name

SMAD2 Polyclonal Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using UHRF2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using UHRF2 at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using UHRF2 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using UHRF2 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using UHRF2 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using UHRF2 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using UHRF2 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat Intestine using UHRF2 at dilution of 1:200 (40x lens).)

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UHRF2, Polyclonal Antibody (Cat# AAA30617)

Full Name

UHRF2 Rabbit Polyclonal Antibody

Gene Names

UHRF2; NIRF; URF2; RNF107

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of RT4 cells using anti-PDXK antibody (AAA19875).Overlay histogram showing RT4 cells stained with AAA19875 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDXK Antibody (AAA19875, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of PDXK using anti-PDXK antibody (AAA19875).PDXK was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PDXK Antibody (AAA19875) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PDXK Antibody (AAA19875) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PDXK Antibody (AAA19875) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PDXK Antibody (AAA19875) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PDXK Antibody (AAA19875) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human RT4 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human SiHa whole cell lysates,Lane 5: rat kidney tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse kidney tissue lysates,After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXK antigen affinity purified polyclonal antibody (#AAA19875) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a DyLight488 Conjugated Goat Anti-Rabbit IgG secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (40 kDa.)

WB (Western Blot) (Figure 2. Western blot analysis of PDXK using anti-PDXK antibody (AAA19875).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: mouse kidney tissue lysates,Lane 4: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXK antigen affinity purified polyclonal antibody (#AAA19875) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (40 kDa.)

WB (Western Blot) (Figure 1. Western blot analysis of PDXK using anti-PDXK antibody (AAA19875).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human SiHa whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDXK antigen affinity purified polyclonal antibody (#AAA19875) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (40 kDa.)

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PDXK, Polyclonal Antibody (Cat# AAA19875)

Full Name

Anti-PDXK Antibody Picoband

Gene Names

PDXK; PKH; PNK; PRED79; C21orf97; C21orf124

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat pancreas using ZNF581 Polyclonal Antibody at dilution of 1:100 (40x lens).)

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ZNF581, Polyclonal Antibody (Cat# AAA22179)

Full Name

ZNF581 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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IHC (Immunohistchemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

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CKR-5, Polyclonal Antibody (Cat# AAA29347)

Full Name

CKR-5 Polyclonal Antibody

Gene Names

CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse testis using PABPC1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal using PABPC1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using PABPC1 at dilution of 1:100 (40x lens).)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using PABPC1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using PABPC1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PABPC1 at 1:1000 dilution.)

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PC1, Polyclonal Antibody (Cat# AAA30627)

Full Name

Rabbit Polyclonal Antibody PC1 Rabbit Polyclonal Antibody

Gene Names

PABPC1; PAB1; PABP; PABP1; PABPC2; PABPL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (At 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of L-plastin (Phospho-Ser5) using 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of L-plastin (Phospho-Ser5) using 293 whole cell lysates)

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Plastin L, Polyclonal Antibody (Cat# AAA31395)

Full Name

Phospho-Plastin L (Ser5) Antibody

Gene Names

LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistochemistry) (Periplakin was detected in paraffin-embedded sections of human oesophagus squama cancer tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistchemistry) (Periplakin was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Periplakin was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Periplakin was detected in paraffin-embedded sections of rat skin tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Periplakin was detected in paraffin-embedded sections of rat lung tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Periplakin was detected in paraffin-embedded sections of mouse lung tissues using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of Periplakin expression in rat stomach extract (lane 1) and HELA whole cell lysates (lane 2). Periplakin at 202KD was detected using rabbit anti- Periplakin Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

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Periplakin, Polyclonal Antibody (Cat# AAA11684)

Full Name

Anti-Periplakin Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

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NFAT5, Polyclonal Antibody (Cat# AAA29345)

Full Name

NFAT5 Polyclonal Antibody

Gene Names

NFAT5; NFATZ; OREBP; NF-AT5; NFATL1; TONEBP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BAZ1B antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAZ1B antibody.)

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BAZ1B, Polyclonal Antibody (Cat# AAA29857)

Full Name

BAZ1B Polyclonal Antibody

Gene Names

BAZ1B; WSTF; WBSCR9; WBSCR10

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of human placenta tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistchemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of rat intestine tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (Cytokeratin 18 was detected in paraffin-embedded sections of mouse intestine tissues using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of Cytokeratin 18 expression in rat liver extract (lane 1), mouse liver extract (lane 2), HELA whole cell lysates (lane 3) and HEPG2 whole cell lysates (lane 4). Cytokeratin 18 at 55KD was detected using rabbit anti- Cytokeratin 18 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

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Cytokeratin 18, Polyclonal Antibody (Cat# AAA11682)

Full Name

Anti-Cytokeratin 18 Antibody

Gene Names

KRT18; K18; CK-18; CYK18

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat oophoroma cells using ZP3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat oophoroma cells using ZP3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse oophoroma cells using ZP3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse oophoroma cells using ZP3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat oophoroma cells using ZP3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat oophoroma cells using ZP3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

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ZP3, Polyclonal Antibody (Cat# AAA22178)

Full Name

ZP3 Polyclonal Antibody

Gene Names

ZP3; ZPC; ZP3A; ZP3B; Zp-3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$300/0.06 mL $415/0.12 mL $635/0.2 mL $1,225/2x0.2 mL $1,785/3x0.2 mL $2,310/4x0.2 mL $2,775/5x0.2 mL

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K4 antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K4 antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K4 antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H3-K4 antibody at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 300s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K4 antibody at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

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Acetyl-Histone H3-K4, Polyclonal Antibody (Cat# AAA28322)

Full Name

Acetyl-Histone H3-K4 Rabbit pAb

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$255/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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WB (Western Blot) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

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Cleaved-Caspase-9, Polyclonal Antibody (Cat# AAA28834)

Full Name

Cleaved-Caspase-9 (D353) Polyclonal Antibody

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

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Annexin I, Polyclonal Antibody (Cat# AAA29346)

Full Name

Annexin I Polyclonal Antibody

Gene Names

ANXA1; ANX1; LPC1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Smarcd1 antibody.)

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BAF60a, Polyclonal Antibody (Cat# AAA29858)

Full Name

BAF60a Polyclonal Antibody

Gene Names

Smarcd1; Baf60a; D15Kz1; AA407987

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of ABCA7 in human spleen tissue with ABCA7 antibody at 20 μg/ml.Red: ABCA7 Antibody (6889)Blue: DAPI staining)

IHC (Immunohistochemistry) (Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5μg/ml.)

ICC (Immunocytochemistry) (Immunocytochemistry of ABCA7 in 293 cells with ABCA7 antibody at 5 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of ABCA7 in 293 cells with ABCA7 antibody at 20 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of ABCA7 in human spleen tissue with ABCA7 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of ABCA7 in 293 cell lysate with ABCA7 antibody at (A) 1 and (B) 2 μg/mL.)

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ABCA7, Polyclonal Antibody (Cat# AAA10915)

Full Name

ABCA7 Antibody

Gene Names

ABCA7; ABCX; ABCA-SSN

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

ABCA7 Antibody is affinity chromatography purified via peptide column.

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

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FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of JK cells using anti-LEO1 antibody (AAA19871).Overlay histogram showing JK cells stained with AAA19871 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LEO1 Antibody (AAA19871, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of LEO1 using anti-LEO1 antibody (AAA19871).LEO1 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LEO1 Antibody (AAA19871) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human MCF-7 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human RT4 whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LEO1 antigen affinity purified polyclonal antibody (#AAA19871) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LEO1 at approximately 105 kDa. The expected band size for LEO1 is at 75 kDa.)

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LEO1, Polyclonal Antibody (Cat# AAA19871)

Full Name

Anti-LEO1 Antibody Picoband

Gene Names

LEO1; RDL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

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Cleaved-Caspase-6 p18, Polyclonal Antibody (Cat# AAA28831)

Full Name

Cleaved-Caspase-6 p18 (D162) Polyclonal Antibody

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

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GABA T-1, Polyclonal Antibody (Cat# AAA29343)

Full Name

GABA T-1 Polyclonal Antibody

Gene Names

SLC6A1; GAT1; GABATR; GABATHG

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CAD antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using CAD antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using CAD antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CAD antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using CAD antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CAD antibody.)

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CAD, Polyclonal Antibody (Cat# AAA29855)

Full Name

CAD Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

IHC (Immunohistchemistry) (Figure 9. IHC analysis of MEP1B using anti-MEP1B antibody (AAA19870).MEP1B was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MEP1B Antibody (AAA19870) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEP1B antigen affinity purified polyclonal antibody (#AAA19870) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MEP1B at approximately 97 kDa. The expected band size for MEP1B is at 80 kDa.)

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MEP1B, Polyclonal Antibody (Cat# AAA19870)

Full Name

Anti-MEP1B Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

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p15, Polyclonal Antibody (Cat# AAA29086)

Full Name

p15 Polyclonal Antibody

Gene Names

CDKN2B; P15; MTS2; TP15; CDK4I; INK4B; p15INK4b

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using VASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using VASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using VASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using VASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using VASP at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using VASP at 1:3000 dilution.)

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VASP, Polyclonal Antibody (Cat# AAA30622)

Full Name

VASP Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis Smad2 (Phospho-Ser255) using heatshock treated HT29 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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Smad2, Polyclonal Antibody (Cat# AAA31390)

Full Name

Phospho-Smad2 (Ser255) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP90AA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using HSP90AA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using HSP90AA1 Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse testis using HSP90AA1 Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using HSP90AA1 Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat testis using HSP90AA1 Polyclonal Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using HSP90AA1 Polyclonal Antibody at dilution of 1:1000.)

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HSP90AA1, Polyclonal Antibody (Cat# AAA22176)

Full Name

HSP90AA1 Polyclonal Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$295/0.06 mL $405/0.12 mL $615/0.2 mL $1,185/2x0.2 mL $1,725/3x0.2 mL $2,235/4x0.2 mL $2,730/5x0.2 mL

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Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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Cleaved-Caspase-7 p20, Polyclonal Antibody (Cat# AAA28832)

Full Name

Cleaved-Caspase-7 p20 (D198) Polyclonal Antibody

Gene Names

CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using TEFM antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using TEFM antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using TEFM antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using TEFM antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using TEFM antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TEFM antibody.)

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TEFM, Polyclonal Antibody (Cat# AAA29856)

Full Name

TEFM Polyclonal Antibody

Gene Names

TEFM; C17orf42

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CAMK1 at 1:3000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using CAMK1 at dilution of 1:100 (40x lens).)

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CAMK1, Polyclonal Antibody (Cat# AAA30624)

Full Name

CAMK1 Rabbit Polyclonal Antibody

Gene Names

CAMK1; CAMKI

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-NR1I2 antibody Titration: 1 ug/mLSample Type: Human heart)

WB (Western Blot) (WB Suggested Anti-NR1I2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Hela cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: NR1I2Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NR1I2Sample Tissue: Human 293T Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NOP56Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Small Intestine tissue at an antibody concentration of 5.0ug/ml using anti-NR1I2 antibody)

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NR1I2, Polyclonal Antibody (Cat# AAA23452)

Full Name

NR1I2 antibody - N-terminal region

Gene Names

NR1I2; BXR; PAR; PRR; PXR; SAR; SXR; ONR1; PAR1; PAR2; PARq

Reactivity

Human

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H2B-K12 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 antibody at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 antibody at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 antibody at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H2B-K12 antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K12 antibody at 1:1000 dilution.HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

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Acetyl-Histone H2B-K12, Polyclonal Antibody (Cat# AAA28316)

Full Name

Acetyl-Histone H2B-K12 Rabbit pAb

Gene Names

HIST2H2BE; H2B; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$255/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using SAFB antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using SAFB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using SAFB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using SAFB antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using SAFB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SAFB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SAFB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using SAFB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using SAFB antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using SAFB antibody.)

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SAFB, Polyclonal Antibody (Cat# AAA29852)

Full Name

SAFB Polyclonal Antibody

Gene Names

SAFB; HAP; HET; SAF-B; SAFB1; SAB-B1; SAF-B1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Human breast tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of p27 Kip1 (Phospho-Ser178) using EGF treated HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of extracts from rat brain, using p27 Kip1 (Phospho-Ser178) Antibody. Lane 1 was treated with the blocking peptide.)

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p27 Kip1, Polyclonal Antibody (Cat# AAA31388)

Full Name

Phospho-p27 Kip1 (Ser178) Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using RhoA Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using RhoA Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using RhoA Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat pancreas using RhoA Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using RhoA Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human placenta using RhoA Polyclonal Antibody at dilution of 1:100 (40x lens).)

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RhoA, Polyclonal Antibody (Cat# AAA22173)

Full Name

RhoA Polyclonal Antibody

Gene Names

RHOA; ARHA; ARH12; RHO12; RHOH12

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$235/0.06 mL $335/0.12 mL $500/0.2 mL $965/2x0.2 mL $1,400/3x0.2 mL $1,810/4x0.2 mL $2,050/5x0.2 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Ret (Phospho-Tyr1096) using EGF treated HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-Ret (Tyr1096) Antibody.Lane 1: PC12(H2O2 treatment), blocked with antigen-specific peptides,Lane 2: PC12(H2O2 treatment),Lane 3: Hela cells(lps treatment).)

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Ret, Polyclonal Antibody (Cat# AAA31389)

Full Name

Phospho-Ret (Tyr1096) Antibody

Gene Names

RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; RET51; CDHF12; CDHR16; RET-ELE1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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WB (Western Blot) (Host: RabbitTarget Name: ACTBSample Type: JurkatAntibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: ACTBSample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ACTBSample Type: HepG2Lane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5.0 ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 12%)

WB (Western Blot) (Host: RabbitTarget Name: ACTBSample Type: HepG2 Whole Cell lysatesAntibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in HepG2)

WB (Western Blot) (Host: RabbitTarget Name: ACTBSample Type: HelaAntibody Dilution: 1.0ug/mlACTB is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

IHC (Immunohistochemistry) (IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml.)

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ACTB, Polyclonal Antibody (Cat# AAA23467)

Full Name

ACTB Antibody - middle region

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Cow, Goat, Horse, Human, Mouse, Pig, Rat, Sheep, Zebrafish

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

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ANG I, Polyclonal Antibody (Cat# AAA29355)

Full Name

ANG I Polyclonal Antibody

Gene Names

ANG; ALS9; HEL168; RNASE4; RNASE5

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Tuberin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (Immunocytochemical staining of PANC-1 cells using anti-Tuberin rabbit polyclonal antibody.)

ICC (Immunocytochemistry) (Immunocytochemical staining of SH-SY-5Y cells using anti-Tuberin rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Tuberin rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded mouse pancreas tissue using anti-Tuberin rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Tuberin rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Tuberin rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human pancreas tissue using anti-Tuberin rabbit polyclonal antibody.)

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TSC2/Tuberin, Polyclonal Antibody (Cat# AAA29867)

Full Name

TSC2/Tuberin Antibody

Gene Names

TSC2; LAM; TSC4

Reactivity

Human, Mouse

Applications

Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide affinity purified

Pricing

$360/0.05 mL $480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,925/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of CIDE-A in human brain tissue with CIDE A antibody at 2.5 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of CIDE-A in human brain tissue with CIDE A antibody at 20 μg/mL.Green: CIDE-A Antibody (2089)Blue: DAPI staining)

IHC (Immunohistochemistry) (Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 2.5 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of CIDE-A in Mouse Heart cells with CIDE-A antibody at 20 ug/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of CIDE-A in mouse heart tissue with CIDE-A antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of CIDE-A in mouse heart tissue lysate with CIDE-A antibody at 1:500 dilution.)

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CIDE-A, Polyclonal Antibody (Cat# AAA10924)

Full Name

CIDE-A Antibody

Gene Names

CIDEA; CIDE-A

Reactivity

Mouse

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

CIDE-A Antibody is affinity chromatography purified via peptide column.

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Thioredoxin reductase 1 (Thioredoxin reductase 1 (TXNRD1 ) ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Thioredoxin reductase 1 (Thioredoxin reductase 1 (TXNRD1 ) ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Thioredoxin reductase 1 (Thioredoxin reductase 1 (TXNRD1 ) ) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using Thioredoxin reductase 1 (Thioredoxin reductase 1 (TXNRD1 ) ) antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using Thioredoxin reductase 1 (Thioredoxin reductase 1 (TXNRD1 ) ) antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Thioredoxin reductase 1 (Thioredoxin reductase 1 (TXNRD1 ) ) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

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Thioredoxin reductase 1 (TXNRD1), Polyclonal Antibody (Cat# AAA28332)

Full Name

Thioredoxin reductase 1 (TXNRD1) Rabbit pAb

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

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pS2, Polyclonal Antibody (Cat# AAA29356)

Full Name

pS2 Polyclonal Antibody

Gene Names

TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21

Reactivity

Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AQP2 at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using AQP2 at dilution of 1:100 (40x lens).)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse kidney cells using AQP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat kidney cells using AQP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse kidney cells using AQP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat kidney cells using AQP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse kidney cells using AQP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat kidney cells using AQP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

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AQP2, Polyclonal Antibody (Cat# AAA30636)

Full Name

AQP2 Rabbit Polyclonal Antibody

Gene Names

AQP2; AQP-CD; WCH-CD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western blot analysis of AF7020 expression in various lysates)

WB (Western Blot) (Western blot analysis of HMGB1 expression in Mouse liver tissue.)

IHC (Immunohistochemistry) (At 1/200 staining human lymphoca tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistchemistry) (At 1/200 staining human lymphoca tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistochemistry) (At 1/200 staining human lymphoca tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistochemistry) (At 1/50 staining human lymphoca tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

IHC (Immunohistochemistry) (HMGB1 Antibody for IHC in human testis)

IF (Immunofluorescence) (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)

IF (Immunofluorescence) (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibod)

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HMGB1, Polyclonal Antibody (Cat# AAA31148)

Full Name

HMGB1 Antibody

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Reactivity

Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Rabbit, Dog

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of JAK3 (Phospho-Tyr904) using SW626 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-JAK3 (Tyr904) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: HepG2 cells(heat shock treatment).)

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JAK3, Polyclonal Antibody (Cat# AAA31404)

Full Name

Phospho-JAK3 (Tyr904) Antibody

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.