Polyclonal Antibodies

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ATF5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ATF5, Polyclonal Antibody (Cat# AAA28366)

Full Name

ATF5 Rabbit pAb

Gene Names

ATF5; ATFX; HMFN0395

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000)

Granzyme B, Polyclonal Antibody (Cat# AAA29390)

Full Name

Granzyme B Polyclonal Antibody

Gene Names

GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (The image is immunohistochemistry of paraffin embedded Human breast cancer tissue using HSD17B13 Antibody at dilution 1/140.)

HSD17B13, Polyclonal Antibody (Cat# AAA29646)

Full Name

HSD17B13 Antibody

Gene Names

HSD17B13; SCDR9; NIIL497; SDR16C3; HMFN0376

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen affinity purification.

Pricing

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ELAVL1, Polyclonal Antibody (Cat# AAA10703)

Full Name

ELAVL1 Polyclonal Antibody

Gene Names

ELAVL1; HUR; Hua; MelG; ELAV1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of MAP1LC3A using anti-MAP1LC3A antibody (AAA19151).MAP1LC3A was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP1LC3A Antibody (AAA19151) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

MAP1LC3A, Polyclonal Antibody (Cat# AAA19151)

Full Name

Anti-MAP1LC3A Picoband antibody

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Pricing

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WB (Western Blot) (WB Suggested Anti-GNAS Antibody Titration: 1 ug/mlPositive Control: MCF-7 whole cell lysatesGNAS is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells)

GNAS, Polyclonal Antibody (Cat# AAA23503)

Full Name

GNAS antibody - N-terminal region

Gene Names

GNAS; AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using HNRNPM antibody at dilution of 1:100 (40x lens).)

HNRNPM, Polyclonal Antibody (Cat# AAA28111)

Full Name

HNRNPM

Gene Names

HNRNPM; CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using CASK antibody. Blue: DAPI for nuclear staining.)

CASK, Polyclonal Antibody (Cat# AAA29647)

Full Name

CASK Antibody

Gene Names

CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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Cyclin E1, Polyclonal Antibody (Cat# AAA31439)

Full Name

Phospho-Cyclin E1 (Ser399) Antibody

Gene Names

CCNE1; CCNE

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bovine (88%), Horse (88%), Sheep (88%), Chicken (88%), Xenopus (88%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western blot analysis of SQSTM1 expression in K562 cell lysate with AP3B2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

SQSTM1, Polyclonal Antibody (Cat# AAA10960)

Full Name

SQSTM1 Antibody

Gene Names

SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

SQSTM1 Antibody is affinity chromatography purified via peptide column.

Pricing

ALPHA 2 MACROGLOBULIN, Polyclonal Antibody (Cat# AAA12240)

Full Name

SHEEP ANTI HUMAN ALPHA 2 MACROGLOBULIN

Gene Names

A2M; A2MD; CPAMD5; FWP007; S863-7

Applications

Immunohistology Frozen, ELISA (EIA)

Pricing

COVID 19 Nucleocapsid (NP) Coronavirus, Polyclonal Antibody (Cat# AAA13776)

Full Name

Anti-NP Protein (SARS-CoV-2) Polyclonal Antibody

Applications

Western Blot (WB), ELISA (EIA)

Purity

Protein G immunoaffinity chromatography

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

VIM, Polyclonal Antibody (Cat# AAA29137)

Full Name

VIM Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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Histone H3, Polyclonal Antibody (Cat# AAA30929)

Full Name

Histone H3 Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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KRT5, Polyclonal Antibody (Cat# AAA10706)

Full Name

KRT5 Polyclonal Antibody

Gene Names

KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-NDUFA4 antibody (AAA19922).Overlay histogram showing K562 cells stained with AAA19922 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA4 Antibody (AAA19922, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of NDUFA4 using anti-NDUFA4 antibody (AAA19922).NDUFA4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA4 Antibody (AAA19922) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA4 Antibody (AAA19922) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-NDUFA4 Antibody (AAA19922) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: rat heart tissue lysates,Lane 5: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA4 antigen affinity purified polyclonal antibody (#AAA19922) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NDUFA4 at approximately 12 kDa. The expected band size for NDUFA4 is at 9 kDa.)

NDUFA4, Polyclonal Antibody (Cat# AAA19922)

Full Name

Anti-NDUFA4 Antibody Picoband

Gene Names

NDUFA4; MLRQ; CI-9k; CI-MLRQ

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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AIMP1, Polyclonal Antibody (Cat# AAA28108)

Full Name

AIMP1 Polyclonal Antibody

Gene Names

AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

TIMP-1, Polyclonal Antibody (Cat# AAA29132)

Full Name

TIMP-1 Polyclonal Antibody

Gene Names

TIMP1; EPA; EPO; HCI; CLGI; TIMP

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

CD22, Polyclonal Antibody (Cat# AAA29388)

Full Name

CD22 Polyclonal Antibody

Gene Names

CD22; SIGLEC2; SIGLEC-2

Reactivity

Human

Applications

Western Blot (WB)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U20S cell using PSME1 antibody. Blue: DAPI for nuclear staining.)

PSME1, Polyclonal Antibody (Cat# AAA29644)

Full Name

PSME1 Antibody

Gene Names

PSME1; PA28A; IFI5111; REGalpha; PA28alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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TPPP, Polyclonal Antibody (Cat# AAA31436)

Full Name

Phospho-TPPP (Ser18) Antibody

Gene Names

TPPP; p24; p25; TPPP1; TPPP/p25; p25alpha

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (89%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of Periostin using anti-Periostin antibody (AAA19149).Periostin was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Periostin Antibody (AAA19149) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Periostin, Polyclonal Antibody (Cat# AAA19149)

Full Name

Anti-Periostin Picoband Antibody

Gene Names

Postn; Plf

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Immunogen affinity purified

Pricing

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DNMT3A, Polyclonal Antibody (Cat# AAA10707)

Full Name

DNMT3A Polyclonal Antibody

Gene Names

DNMT3A; TBRS; DNMT3A2; M.HsaIIIA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-PTPIP51/RMDN3 antibody (AAA19923).Overlay histogram showing HepG2 cells stained with AAA19923 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PTPIP51/RMDN3 using anti-PTPIP51/RMDN3 antibody (AAA19923).PTPIP51/RMDN3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTPIP51/RMDN3 Antibody (AAA19923) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPIP51/RMDN3 antigen affinity purified polyclonal antibody (#AAA19923) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTPIP51/RMDN3 at approximately 55 kDa. The expected band size for PTPIP51/RMDN3 is at 52 kDa.)

PTPIP51/RMDN3, Polyclonal Antibody (Cat# AAA19923)

Full Name

Anti-PTPIP51/RMDN3 Antibody Picoband

Gene Names

RMDN3; RMD3; RMD-3; FAM82C; FAM82A2; ptpip51

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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Cytokeratin AE1, Polyclonal Antibody (Cat# AAA28627)

Full Name

Cytokeratin AE1 Rabbit pAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using HNMT antibody. Blue: DAPI for nuclear staining.)

HNMT, Polyclonal Antibody (Cat# AAA29651)

Full Name

HNMT Antibody

Gene Names

HNMT; HMT; HNMT-S1; HNMT-S2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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WB (Western Blot) (WB Suggested Anti-KRT8 Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysateKRT8 is supported by BioGPS gene expression data to be expressed in HepG2)

KRT8, Polyclonal Antibody (Cat# AAA23499)

Full Name

KRT8 antibody - middle region

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Tie-1, Polyclonal Antibody (Cat# AAA29131)

Full Name

Tie-1 Polyclonal Antibody

Gene Names

TIE1; TIE; JTK14

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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LRPPRC, Polyclonal Antibody (Cat# AAA30667)

Full Name

LRPPRC Rabbit Polyclonal Antibody

Gene Names

LRPPRC; LSFC; GP130; LRP130; CLONE-23970

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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SRF, Polyclonal Antibody (Cat# AAA31435)

Full Name

Phospho-SRF (Thr159) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using MAP2K3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

MAP2K3, Polyclonal Antibody (Cat# AAA22220)

Full Name

MAP2K3 Polyclonal Antibody

Gene Names

MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using RPS7 antibody at dilution of 1:100 (40x lens).)

RPS7, Polyclonal Antibody (Cat# AAA28112)

Full Name

RPS7 Polyclonal Antibody

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

VEGF-D, Polyclonal Antibody (Cat# AAA29136)

Full Name

VEGF-D Polyclonal Antibody

Gene Names

FIGF; VEGFD; VEGF-D

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TRAP1 at 1:3000 dilution.)

TRAP1, Polyclonal Antibody (Cat# AAA30672)

Full Name

TRAP1 Rabbit Polyclonal Antibody

Gene Names

TRAP1; HSP75; HSP 75; HSP90L; TRAP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using GGA2 antibody. Blue: DAPI for nuclear staining.)

GGA2, Polyclonal Antibody (Cat# AAA10705)

Full Name

GGA2 Polyclonal Antibody

Gene Names

GGA2; VEAR

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IRS-1, Polyclonal Antibody (Cat# AAA29050)

Full Name

IRS-1 Polyclonal Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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p53, Polyclonal Antibody (Cat# AAA31354)

Full Name

Acetyl-p53 (Lys373) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

PDPK1, Polyclonal Antibody (Cat# AAA11643)

Full Name

Anti-PDPK1 Antibody

Gene Names

PDPK1; PDK1; PDPK2; PDPK2P; PRO0461

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.)

MAP1LC3B, Polyclonal Antibody (Cat# AAA28283)

Full Name

MAP1LC3B Polyclonal Antibody

Gene Names

MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

PAK1, Polyclonal Antibody (Cat# AAA31096)

Full Name

PAK1 Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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IHC (Immunohistochemistry) (Anti- Annexin A3 Picoband antibody, AAA11641, IHC(F)IHC(F): Human Placenta Tissue)

Annexin A3, Polyclonal Antibody (Cat# AAA11641)

Full Name

Anti-Annexin A3 Antibody

Gene Names

ANXA3; ANX3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin

Purity

Immunogen Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Hela cells using anti-RTCB antibody (AAA19833).Overlay histogram showing Hela cells stained with AAA19833 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTCB Antibody (AAA19833, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RTCB using anti-RTCB antibody (AAA19833).RTCB was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RTCB Antibody (AAA19833) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Jurkat whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (#AAA19833) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.)

RTCB, Polyclonal Antibody (Cat# AAA19833)

Full Name

Anti-RTCB Antibody Picoband

Gene Names

RTCB; FAAP; HSPC117; C22orf28; DJ149A16.6

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Integrin alphaV, Polyclonal Antibody (Cat# AAA29049)

Full Name

Integrin alphaV Polyclonal Antibody

Gene Names

ITGAV; CD51; MSK8; VNRA; VTNR

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of C9JLR9 Antibody (C-term) (AAA28790) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

C9JLR9, Polyclonal Antibody (Cat# AAA28790)

Full Name

C9JLR9 Antibody (C-term)

Reactivity

Human
Predicted Reactivity: Mouse

Applications

Western Blot (WB), ELISA, Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)