Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.)

WB (Western Blot) (Western blot analysis of HLA-B Antibody (N-term) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of HLA-B Antibody (N-term) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term). AAA28743 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term). AAA28743 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

FCM (Flow Cytometry) (Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). AAA28743 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

FCM (Flow Cytometry) (Flow cytometric analysis of human peripheral blood lymphocytes cells using HLA-B Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). AAA28743 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Compare (5 max)

HLA-B, Polyclonal Antibody (Cat# AAA28743)

Full Name

HLA-B Antibody (N-term)

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

Tubulin alpha, Polyclonal Antibody (Cat# AAA29255)

Full Name

Tubulin alpha Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Compare (5 max)

CREB, Polyclonal Antibody (Cat# AAA31303)

Full Name

Phospho-CREB (Ser129) Antibody

Gene Names

CREB1; CREB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

MYH-pan, Polyclonal Antibody (Cat# AAA29253)

Full Name

MYH-pan Polyclonal Antibody

Gene Names

MYH1; MYHa; HEL71; MYHSA1; MyHC-2x; MyHC-2X/D

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human lung cancer tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistchemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded rat brain tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded rat heart tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistchemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human lung cancer tissue.)

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human esophagus.)

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human colon tissue.)

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human embryo brain tissue using MTR4 antibody at dilution of 1:200 (x400 lens).)

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Immunohistochemistry of paraffin-embedded human rectal cancer tissue using MTR4 antibody at dilution of 1:200 (x400 lens))

IHC (Immunohistochemistry) (SKIV2L2 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

Compare (5 max)

SKIV2L2, Polyclonal Antibody (Cat# AAA21318)

Full Name

SKIV2L2 Rabbit anti-Human Polyclonal Antibody

Reactivity

Mouse, Human

Applications

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Affinity purified

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of CARD6 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CARD6 (arrow) was detected using the purified Pab.)

WB (Western Blot) (CARD6 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CARD6 antibody detected the CARD6 protein (arrow).)

WB (Western Blot) (Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 20ug.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Compare (5 max)

CARD6, Polyclonal Antibody (Cat# AAA28742)

Full Name

CARD6 Antibody (Center)

Gene Names

CARD6; CINCIN1

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using PSMA Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Rat brain using PSMA Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using PSMA Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse brain using PSMA Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human kidney cancer using PSMA Polycloanl Antibody at dilution of 1:200)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human prostate cancer using PSMA Polycloanl Antibody at dilution of 1:200)

WB (Western Blot) (Western blot with PSMA Polyclonal Antibody at dilution of 1:1000.lane 1:LNCaP whole cell lysate)

Compare (5 max)

PSMA, Polyclonal Antibody (Cat# AAA22343)

Full Name

PSMA Polyclonal Antibody

Gene Names

Folh1; GCP2; mopsm

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Antigen Affinity Purification

Pricing

$200/0.025 mL $350/0.1 mL $670/2x0.1 mL $970/3x0.1 mL $1,255/4x0.1 mL $1,510/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1,Caspase-7 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-breast-cancer tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-breast-cancer tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-breast-cancer tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-liver-cancer tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-liver-cancer tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-liver-cancer tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-lung tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-lung tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-lung tissue. 1,Caspase-7 Polyclonal Antibody(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

WB (Western Blot) (Western Blot analysis of various cells using Caspase-7 Polyclonal Antibody diluted at 1:1000)

Compare (5 max)

Caspase-7, Polyclonal Antibody (Cat# AAA30784)

Full Name

Caspase-7 Rabbit Polyclonal Antibody

Gene Names

CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3

Reactivity

Human, Rat, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$305/0.05 mL $385/0.1 mL $740/2x0.1 mL $1,070/3x0.1 mL $1,385/4x0.1 mL $1,475/5x0.1 mL

< >

<< Previous Next >>

Application Data (ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)

WB (Western Blot) (Western blot analysis on F9 (A)and NCCIT (B) cell lysates using anti-LGR5 rabbit polyclonal antibody.)

Compare (5 max)

LGR5, Polyclonal Antibody (Cat# AAA17985)

Full Name

LGR5 Antibody

Gene Names

LGR5; FEX; HG38; GPR49; GPR67; GRP49

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC)

Purity

Immunogen affinity purified

Pricing

$385/0.1 mL $695/0.1 mL (AF350) $695/0.1 mL (AF405L) $695/0.1 mL (AF405M) $695/0.1 mL (AF405S) $695/0.1 mL (AF488) $695/0.1 mL (AF546) $695/0.1 mL (AF555) $695/0.1 mL (AF568) $695/0.1 mL (AF594) $695/0.1 mL (AF610) $695/0.1 mL (AF635) $695/0.1 mL (AF647) $695/0.1 mL (AF680) $695/0.1 mL (AF750) $695/0.1 mL (AP) $695/0.1 mL (APC) $695/0.1 mL (Biotin) $695/0.1 mL (Cy3) $695/0.1 mL (Cy5) $695/0.1 mL (Cy5.5) $695/0.1 mL (Cy7) $695/0.1 mL (FITC) $695/0.1 mL (HRP) $695/0.1 mL (PE) $1,345/2x0.1 mL (AF350) $1,955/3x0.1 mL (AF350) $2,535/4x0.1 mL (AF350)

< >

<< Previous Next >>

Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Compare (5 max)

RPA32/RPA2, Polyclonal Antibody (Cat# AAA31304)

Full Name

Phospho-RPA32/RPA2 (Ser4/Ser8) Antibody

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Hspb7 was immunoprecipitated using: Lane A:0.5 mg NIH-3T3 Whole Cell Lysate Lane B:0.5 mg A549 Whole Cell Lysate 4 uL anti-Hspb7 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Hspb7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa Observed band size: 22 kDa)

IHC (Immunohistochemistry) (Immunochemical staining Hspb7 in rat heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining Hspb7 in rat skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining Hspb7 in mouse heart with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining Hspb7 in mouse skeletal muscle with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

WB (Western Blot) (Anti-Hspb7 rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse heart tissue lysate Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:19 kDa Observed band size:19 kDa (We are unsure as to the identity of these extra bands.))

Compare (5 max)

HSPB7, Polyclonal Antibody (Cat# AAA27732)

Full Name

Anti-HSPB7 Antibody, Rabbit Polyclonal

Gene Names

Hspb7; 27kDa; cvHsp; Hsp25-2

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CDKN1A antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CDKN1A Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using CDKN1A Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using CDKN1A Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CDKN1A Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using CDKN1A Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using CDKN1A Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CDKN1A Antibody at dilution of 1:100 (40x lens).)

Compare (5 max)

CDKN1A, Polyclonal Antibody (Cat# AAA28244)

Full Name

CDKN1A Polyclonal Antibody

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

DNAM-1, Polyclonal Antibody (Cat# AAA29012)

Full Name

DNAM-1 Polyclonal Antibody

Gene Names

CD226; PTA1; DNAM1; DNAM-1; TLiSA1

Reactivity

Human, Mouse, Monkey

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using BRCA1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BRCA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Compare (5 max)

BRCA1, Polyclonal Antibody (Cat# AAA28245)

Full Name

BRCA1 Polyclonal Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Applications: Western BlotSample: Rat PFCDetection: Protein Simple, Simple Western size-based assay"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate.")

Application Data (Anti-CD10 Picoband antibody, AAA11606-5.jpgAll lanes: Anti-CD10(AAA11606) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 40ugLane 3: Rat Liver Tissue Lysate at 40ugLane 4: Human Placenta Tissue Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: JURKAT Whole Cell Lysate at 40ugLane 7: RAJI Whole Cell Lysate at 40ugLane 8: 293T Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 100KD)

IHC (Immunohistochemistry) (Anti-CD10 Picoband antibody, AAA11606-4.JPGIHC(P): Rat Spleen Tissue)

IHC (Immunohistochemistry) (Anti-CD10 Picoband antibody, AAA11606-3.JPGIHC(P): Mouse Spleen Tissue)

IHC (Immunohistochemistry) (Anti-CD10 Picoband antibody, AAA11606-2.JPGIHC(P): Human B lymphocyte Cancer Tissue)

IHC (Immunohistochemistry) (Anti-CD10 Picoband antibody, AAA11606-1.JPGIHC(P): Mouse Kidney Tissue)

Compare (5 max)

CD10, Polyclonal Antibody (Cat# AAA11606)

Full Name

Anti-CD10 Antibody

Gene Names

MME; NEP; SFE; CD10; CALLA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (AAA19798).Overlay histogram showing HepG2 cells stained with AAA19798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (AAA19798, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IP (Immunoprecipitation) (Figure 7. Immunoprecipitating RPS14 in Hela whole cell lysate .Western blot analysis of RPS14 using anti-RPS14 antibody (AAA19798).Lane 1: Hela whole cell lysates (30ug)Lane 2: Rabbit control IgG instead of anti-RPS14 antibody in Hela whole cell lysate.Lane 3: anti-RPS14 antibody (2ug) + Hela whole cell lysate (500ug)After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (AAA19798) at a dilution of 0.5ug/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS14 Antibody (AAA19798) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates,Lane 4: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,Lane 5: rat liver tissue lysates,Lane 6: rat RH35 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS14 antigen affinity purified polyclonal antibody (#AAA19798) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS14 at approximately 16 kDa. The expected band size for RPS14 is at 16 kDa.)

Compare (5 max)

RPS14, Polyclonal Antibody (Cat# AAA19798)

Full Name

Anti-RPS14 Antibody Picoband

Gene Names

RPS14; S14; EMTB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunochemical staining of human RAB1A in rat small intestine with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistchemistry) (Immunochemical staining of human RAB1A in rat kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human RAB1A in mouse small intestine with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human RAB1A in mouse kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human RAB1A in human small intestine with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human RAB1A in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-RAB1A rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:23 kDa)

Compare (5 max)

RAB1A, Polyclonal Antibody (Cat# AAA27738)

Full Name

Anti-RAB1A Antibody, Rabbit Polyclonal

Gene Names

RAB1A; RAB1; YPT1

Reactivity

Human
Predicted to React with: Pig (Species predicted to react based on 100% sequence homology)

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human prostate using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SET antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using SET antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SET antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Compare (5 max)

SET, Polyclonal Antibody (Cat# AAA28250)

Full Name

SET Polyclonal Antibody

Gene Names

SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

EGFR, Polyclonal Antibody (Cat# AAA29018)

Full Name

EGFR Polyclonal Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunochemical staining NRP1 in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in rat liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in rat brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in mouse brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Compare (5 max)

Neuropilin-1, Polyclonal Antibody (Cat# AAA27735)

Full Name

Anti-Neuropilin-1 Antibody, Rabbit Polyclonal

Gene Names

Nrp1; Nrp; NP-1; Npn1; NPN-1; C530029I03

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)

FCM (Flow Cytometry) (S100B Antibody (Cat. #AAA28759) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of lysates from A431 cell line, mouse brain, rat brain tissue (from left to right), using S100B Antibody(Cat. #AAA28759). AAA28759 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (All lanes : Anti-S100B Antibody at 1:2000 dilution Lane 1: Human brain lysate Lane 2: C2C12 whole cell lysate Lane 3: Rat brain lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast tissue using AAA28759 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human melanoma tissueusing AAA28759 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)

WB (Western Blot) (Anti-S100B Antibody at 1:2000 dilution + Human brain whole tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

S100B, Polyclonal Antibody (Cat# AAA28759)

Full Name

S100B Antibody

Gene Names

S100B; NEF; S100; S100-B; S100beta

Reactivity

Human, Mouse, Rat (Predicted: Rabbit)

Applications

Western Blot (WB), Immunohistochemistry Paraffin Leica (IHC-P-Leica), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), ELISA (EIA)

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

Dynein LC 1, Polyclonal Antibody (Cat# AAA29015)

Full Name

Dynein LC 1 Polyclonal Antibody

Gene Names

DNAL1; CILD16; C14orf168

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse liver using CHD4 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human oophoroma using CHD4 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat liver using CHD4 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of U20S cells using CHD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CHD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using CHD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CHD4 antibody at 1:1000 dilution.)

Compare (5 max)

CHD4, Polyclonal Antibody (Cat# AAA30551)

Full Name

CHD4 Polyclonal Antibody

Gene Names

CHD4; Mi-2b; Mi2-BETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

< >

<< Previous Next >>

Application Data (Anti-ICAM1 Picoband antibody, AAA11608-6.jpgAll lanes: Anti-ICAM1(AAA11608) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 40ugLane 2: Rat Spleen Tissue Lysate at 40ugLane 3: PC12 Whole Cell Lysate at 40ugLane 4: NRK Whole Cell Lysate at 40ugLane 5: RH35 Whole Cell Lysate at 40ugPredicted bind size: 58KDObserved bind size: 90KD)

IHC (Immunohistochemistry) (Anti-ICAM1 Picoband antibody, AAA11608-5.JPGIHC(P): Rat Spleen Tissue)

IHC (Immunohistochemistry) (Anti-ICAM1 Picoband antibody, AAA11608-4.JPGIHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti-ICAM1 Picoband antibody, AAA11608-3.JPGIHC(P): Mouse Spleen Tissue)

IHC (Immunohistochemistry) (Anti-ICAM1 Picoband antibody, AAA11608-2.JPGIHC(P): Mouse Lung Tissue)

IHC (Immunohistochemistry) (Anti-ICAM1 Picoband antibody, AAA11608-1.JPGIHC(P): Mouse Intestine Tissue)

Compare (5 max)

ICAM1, Polyclonal Antibody (Cat# AAA11608)

Full Name

Anti-ICAM1 antibody

Gene Names

Icam1; CD54; Ly-47; Icam-1; MALA-2

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U2OS cells using anti-RBP1 antibody (AAA19800).Overlay histogram showing U2OS cells stained with AAA19800 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBP1 Antibody (AAA19800, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 7. IF analysis of RBP1 using anti-RBP1 antibody (AAA19800) and anti-Tubulin Alpha antibody (M03989-3).RBP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RBP1 Antibody (AAA19800) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP1 antigen affinity purified polyclonal antibody (#AAA19800) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RBP1 at approximately 16 kDa. The expected band size for RBP1 is at 16 kDa.)

Compare (5 max)

RBP1, Polyclonal Antibody (Cat# AAA19800)

Full Name

Anti-RBP1 Antibody Picoband

Gene Names

RBP1; CRBP; RBPC; CRBP1; CRBPI; CRABP-I

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunochemical staining of human SNAP25 in rat liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistchemistry) (Immunochemical staining of human SNAP25 in rat brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human SNAP25 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human SNAP25 in mouse brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human SNAP25 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human SNAP25 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-SNAP25 rabbit polyclonal antibody at 1:500 dilution Lane A: SH-SY5Y Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:23 kDa Observed band size:26 kDa)

Compare (5 max)

SNAP-25, Polyclonal Antibody (Cat# AAA27737)

Full Name

Anti-SNAP-25 Antibody, Rabbit Polyclonal

Gene Names

SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2

Reactivity

Human
Predicted to React with: Bovine, Chick, Pongo abelii (Species predicted to react based on 100% sequence homology)

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody at dilution of 1:100 (20x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using RPN1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RPN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Compare (5 max)

RPN1, Polyclonal Antibody (Cat# AAA28249)

Full Name

RPN1 Polyclonal Antibody

Gene Names

RPN1; OST1; RBPH1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

E-cadherin, Polyclonal Antibody (Cat# AAA29017)

Full Name

E-cadherin Polyclonal Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293T cells Serum-starve overnight, using Phospho-4E-BP1 (Thr37/46) Antibody.Lane1 was treated with phospho-blocking peptide,Lane2 was treated with non-phospho-blocking peptide.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-4E-BP1 (Thr37/Thr46) Antibody.Lane 1: B16F10 cells(UV treatment), blocked with antigen-specific peptides,Lane 2: B16F10 cells(UV treatment),Lane 3: P19 cells(serum starvation treatment).Observed bands: 17kDa)

Compare (5 max)

4E-BP1, Polyclonal Antibody (Cat# AAA31321)

Full Name

Phospho-4E-BP1 (Thr37/Thr46) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using MAP1LC3A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using MAP1LC3A antibody at dilution of 1:100 (20x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using MAP1LC3A antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Compare (5 max)

MAP1LC3A, Polyclonal Antibody (Cat# AAA28243)

Full Name

MAP1LC3A Polyclonal Antibody

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human Spleen tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with AAA28755 KLF4 (N-term C74) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA28755 KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.)

IF (Immunofluorescence) (Fluorescent confocal image of HeLa cells stained with AAA28755 KLF4 (N-term C74) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA28755 KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). KLF4 immunoreactivity is localized to the nuclei in HeLa cells.)

WB (Western Blot) (Western blot analysis of KLF4 Antibody (N-term C74) in mouse stomach tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of KLF4 Antibody (N-term C74) in Jurkat,293 cell line lysates (35ug/lane).KLF4 (arrow) was detected using the purified Pab.)

Compare (5 max)

KLF4, Polyclonal Antibody (Cat# AAA28755)

Full Name

KLF4 Antibody (N-term C74)

Gene Names

KLF4; EZF; GKLF

Reactivity

Human, mouse

Applications

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

EMMPRIN, Polyclonal Antibody (Cat# AAA29267)

Full Name

EMMPRIN Polyclonal Antibody

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of mouse lung using TLR2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using TLR2 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse islets of langerhans using TLR2 antibody at dilution of 1:150 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TLR2 antibody at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat lung using TLR2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using TLR2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Compare (5 max)

TLR2, Polyclonal Antibody (Cat# AAA30547)

Full Name

TLR2 Polyclonal Antibody

Gene Names

TLR2; TIL4; CD282

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: VERO cells(serum starvation treatment).)

Compare (5 max)

RSK1/2/3/4, Polyclonal Antibody (Cat# AAA31315)

Full Name

Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

< >

<< Previous Next >>

Application Data (Anti-Hsp90-alpha-Picoband antibody, AAA11604-6.jpgAll lanes: Anti-HSP90(AAA11604) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 40ugLane 2: Hela Whole Cell Lysate at 40ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: Raji Whole Cell Lysate at 40ugPredicted bind size: 83KDObserved bind size: 83KD)

ICC (Immunocytochemistry) (Anti-Hsp90-alpha-Picoband antibody, AAA11604-5.JPGICC : MCF-7 Cell)

ICC (Immunocytochemistry) (Anti-Hsp90-alpha-Picoband antibody, AAA11604-4.JPGICC: A549 Cell)

IHC (Immunohistochemistry) (Anti-Hsp90-alpha-Picoband antibody, AAA11604-3.JPGIHC(P): Rat Brain Tissue)

IHC (Immunohistochemistry) (Anti-Hsp90-alpha-Picoband antibody, AAA11604-2.JPGIHC(P): Mouse Brain Tissue)

IHC (Immunohistochemistry) (Anti-Hsp90-alpha-Picoband antibody, AAA11604-1.JPGIHC(P): Human Intestinal Cancer Tissue)

Compare (5 max)

Hsp90, Polyclonal Antibody (Cat# AAA11604)

Full Name

Anti-Hsp90 Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

NKp44, Polyclonal Antibody (Cat# AAA29270)

Full Name

NKp44 Polyclonal Antibody

Gene Names

NCR2; LY95; CD336; NKP44; NK-p44; dJ149M18.1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse testis using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using BRCA1 antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BRCA1 antibody at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using BRCA1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using BRCA1 antibody at dilution of 1:100 .)

Compare (5 max)

BRCA1, Polyclonal Antibody (Cat# AAA30550)

Full Name

BRCA1 Polyclonal Antibody

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Anti-Desmin Picoband antibody, AAA11607-7.JPGIHC(P): Rat Cardiac Muscle Tissue)

IHC (Immunohistchemistry) (Anti-Desmin Picoband antibody, AAA11607-6.JPGIHC(P): Mouse Skeletal Muscle Tissue)

IHC (Immunohistochemistry) (Anti-Desmin Picoband antibody, AAA11607-5.JPGIHC(P): Human Intestinal Cancer Tissue)

IHC (Immunohistochemistry) (Anti-Desmin Picoband antibody, AAA11607-4.JPGIHC(F): Mouse Cardiac Muscle Tissue)

IHC (Immunohistochemistry) (Anti-Desmin Picoband antibody, AAA11607-3.JPGIHC(F): Rat Cardiac Muscle Tissue)

Application Data (Anti-Desmin Picoband antibody, AAA11607-2.jpgAll lanes: Anti Desmin (AAA11607) at 0.5ug/mlLane 1: Rat Skeletal Muscle Tissue Lysate at 50ugLane 2: Rat Cardiac MuscleTissue Lysate at 50ugLane 3: Mouse Skeletal Muscle Tissue Lysate at 50ugLane 4: Mouse Cardiac MuscleTissue Lysate at 50ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: HT1080 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: HEPA Whole Cell Lysate at 40ugLane 9: NIH3T3 Whole Cell Lysate at 40ugPredicted bind size: 53KDObserved bind size: 53KD)

Application Data (Anti-Desmin Picoband antibody, AAA11607-1.jpgAll lanes: Anti Desmin (AAA11607) at 0.5ug/mlWB: Recombinant Human Desmin Protein 0.5ngPredicted bind size: 36KDObserved bind size: 36KD)

Compare (5 max)

Desmin, Polyclonal Antibody (Cat# AAA11607)

Full Name

Anti-Desmin Antibody

Gene Names

DES; CSM1; CSM2; LGMD2R

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of A431 cells using anti-BAP31/BCAP31 antibody (AAA19799).Overlay histogram showing A431 cells stained with AAA19799 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP31/BCAP31 Antibody (AAA19799, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of BAP31/BCAP31 using anti-BAP31/BCAP31 antibody (AAA19799).BAP31/BCAP31 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A431 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human placenta tissue lysates,Lane 5: rat liver tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP31/BCAP31 antigen affinity purified polyclonal antibody (#AAA19799) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAP31/BCAP31 at approximately 28 kDa. The expected band size for BAP31/BCAP31 is at 28 kDa.)

Compare (5 max)

BAP31/BCAP31, Polyclonal Antibody (Cat# AAA19799)

Full Name

Anti-BAP31/BCAP31 Antibody Picoband

Gene Names

BCAP31; CDM; BAP31; 6C6-AG; DXS1357E

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

E-cadherin, Polyclonal Antibody (Cat# AAA29016)

Full Name

E-cadherin Polyclonal Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (CDH11/Cadherin 11 Antibody-Immunohistochemistry of paraffin-embedded human kidney using antibody at 1:100 dilution.)

IHC (Immunohistochemistry) (CDH11/Cadherin 11 Antibody-Immunohistochemistry of paraffin-embedded human placenta tissue using antibody at 1:100 dilution.)

IHC (Immunohistochemistry) (CDH11/Cadherin 11 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml)

WB (Western Blot) (CDH11/Cadherin 11 Antibody-Immunoprecipitating CDH11 in SH-SY5Y whole cell lysate Lane 1: Rabbit control IgG instead of CDH11 Antibody in SH-SY5Y whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CDH11 Antibody (8ug) + SH-SY5Y whole cell lysate (500ug) Lane 3: SH-SY5Y whole cell lysate (20ug))

WB (Western Blot) (CDH11/Cadherin 11 Antibody-Western blot. All lanes: CDH11 antibody at 6 ug/ml+Jurkat whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 88 kDa. Observed band size: 88 kDa.)

WB (Western Blot) (CDH11/Cadherin 11 Antibody-Western blot All lanes: CDH11 antibody at 6ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 88, 77 kDa Observed band size: 88 kDa)

Compare (5 max)

CDH11/Cadherin 11, Polyclonal Antibody (Cat# AAA21337)

Full Name

CDH11/Cadherin 11 Rabbit anti-Human Polyclonal (aa637-796) Antibody

Gene Names

CDH11; OB; CAD11; CDHOB; OSF-4

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAG3 antibody at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using BAG3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using BAG3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using BAG3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using BAG3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody at dilution of 1:100 .)

Compare (5 max)

BAG3, Polyclonal Antibody (Cat# AAA30572)

Full Name

BAG3 Polyclonal Antibody

Gene Names

BAG3; BIS; BAG-3; CAIR-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293, using E2F-2 Antibody.)

Compare (5 max)

E2F-2, Polyclonal Antibody (Cat# AAA31340)

Full Name

E2F-2 Antibody

Gene Names

E2F2; E2F-2

Reactivity

Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

IF (Immunofluorescence) (Figure 10. IF analysis of H2AFY/MACROH2A1 using anti-H2AFY/MACROH2A1 antibody (AAA19821) and anti-Beta Tubulin antibody (M01857-3).H2AFY/MACROH2A1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-H2AFY/MACROH2A1 Antibody (AAA19821) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Caco-2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human 293T whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat thymus tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY/MACROH2A1 antigen affinity purified polyclonal antibody (#AAA19821) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for H2AFY/MACROH2A1 at approximately 39 kDa. The expected band size for H2AFY/MACROH2A1 is at 40 kDa.)

Compare (5 max)

H2AFY/MACROH2A1, Polyclonal Antibody (Cat# AAA19821)

Full Name

Anti-H2AFY/MACROH2A1 Antibody Picoband

Gene Names

H2AFY; H2A.y; H2A/y; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat oophoroma cells using ZP3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using ZP3 antibody.)

Compare (5 max)

ZP3, Polyclonal Antibody (Cat# AAA29805)

Full Name

ZP3 Polyclonal Antibody

Gene Names

ZP3; ZPC; ZP3A; ZP3B; Zp-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)

Compare (5 max)

Glut1, Polyclonal Antibody (Cat# AAA29030)

Full Name

Glut1 Polyclonal Antibody

Gene Names

SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT1DS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using GANAB antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using GANAB antibody at dilution of 1:150 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GANAB antibody at dilution of 1:150 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GANAB antibody at 1:3000 dilution.)

Compare (5 max)

GANAB, Polyclonal Antibody (Cat# AAA30566)

Full Name

GANAB Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Annexin II Antibody for IHC in human colon tissue)

WB (Western Blot) (Western blot analysis of Annexin II expression in HeLa cells, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA31078 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31078 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from rat brain, mouse brain, mouse muscle, rat liver, using Annexin II Antibody.)

WB (Western Blot) (Western blot analysis of extracts of mouse liver, using Annexin II antibody.)

Compare (5 max)

Annexin II, Polyclonal Antibody (Cat# AAA31078)

Full Name

Annexin II Antibody

Gene Names

ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse ovary tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human normal tissues adjacent to liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human normal tissues adjacent to mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extract from K-562, Hela, JK and 293 cells using LC3A Antibody)

Compare (5 max)

LC3A, Polyclonal Antibody (Cat# AAA31334)

Full Name

LC3A Antibody

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.