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IHC (Immunohistchemistry) (CDH1/E Cadherin Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

CDH1/E Cadherin, Polyclonal Antibody (Cat# AAA21351)

Full Name

CDH1/E Cadherin Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Reactivity

Zebrafish, Pig, Rat, Rabbit, Dog, Bovine, Human, Monkey

Applications

Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Immunoaffinity purified

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Twist2, Polyclonal Antibody (Cat# AAA31341)

Full Name

Twist2 Antibody

Gene Names

TWIST2; AMS; FFDD3; BBRSAY; DERMO1; SETLSS; bHLHa39

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (92%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of MCF-7 cells using AAA18542 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Glyceraldehyde-3-phosphate dehydrogenase, Polyclonal Antibody (Cat# AAA18542)

Full Name

Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (AAA19822).Rab4/RAB4A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab4/RAB4A antigen affinity purified polyclonal antibody (#AAA19822) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rab4/RAB4A at approximately 24 kDa. The expected band size for Rab4/RAB4A is at 24 kDa.)

Rab4/RAB4A, Polyclonal Antibody (Cat# AAA19822)

Full Name

Anti-Rab4/RAB4A Antibody Picoband

Gene Names

RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (WB Suggested Anti-ATOH1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Placenta)

ATOH1, Polyclonal Antibody (Cat# AAA23406)

Full Name

ATOH1 antibody - middle region

Gene Names

ATOH1; ATH1; HATH1; MATH-1; bHLHa14

Reactivity

Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IL-6Ralpha, Polyclonal Antibody (Cat# AAA29294)

Full Name

IL-6Ralpha Polyclonal Antibody

Gene Names

IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody at dilution of 1:100 .)

KRT14, Polyclonal Antibody (Cat# AAA30574)

Full Name

KRT14 Polyclonal Antibody

Gene Names

KRT14; K14; NFJ; CK14; EBS3; EBS4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

GAPDH, Polyclonal Antibody (Cat# AAA28776)

Full Name

GAPDH Antibody (N-term)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human

Applications

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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WB (Western Blot) (Western blot analysis of Catenin 1 Antibody expression in MCF-7 cells lysates.The lane on the left is treated with the antigen-specific peptide..The lane on the left is treated with the antigen-specific peptide.)

Catenin alpha1, Polyclonal Antibody (Cat# AAA31080)

Full Name

Catenin alpha1 Antibody

Gene Names

CTNNA1; MDPT2; CAP102

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U251 cells using anti-RPS5 antibody (AAA19817).Overlay histogram showing U251 cells stained with AAA19817 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS5 Antibody (AAA19817, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of RPS5 using anti-RPS5 antibody (AAA19817).RPS5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse ovary tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS5 antigen affinity purified polyclonal antibody (#AAA19817) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS5 at approximately 23 kDa. The expected band size for RPS5 is at 23 kDa.)

RPS5, Polyclonal Antibody (Cat# AAA19817)

Full Name

Anti-RPS5 Antibody Picoband

Gene Names

RPS5; S5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (CANX/Calnexin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

CANX/Calnexin, Polyclonal Antibody (Cat# AAA21353)

Full Name

CANX/Calnexin Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names

CANX; CNX; P90; IP90

Reactivity

Mouse, Dog, Bovine, Rat, Human

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Immunoaffinity purified

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Ly6g, Polyclonal Antibody (Cat# AAA22121)

Full Name

Ly6g Polyclonal Antibody

Reactivity

Mouse

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-HNF4A antibody Titration: 1 ug/mLSample Type: Human Liver)

HNF4A, Polyclonal Antibody (Cat# AAA23401)

Full Name

HNF4A Antibody - N-terminal region

Gene Names

HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

GCP5, Polyclonal Antibody (Cat# AAA29029)

Full Name

GCP5 Polyclonal Antibody

Gene Names

TUBGCP5; GCP5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

TRPC3, Polyclonal Antibody (Cat# AAA29285)

Full Name

TRPC3 Polyclonal Antibody

Gene Names

TRPC3; TRP3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

GNL3L, Polyclonal Antibody (Cat# AAA29031)

Full Name

GNL3L Polyclonal Antibody

Gene Names

GNL3L; RP11-353K22.1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

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ESRRA, Polyclonal Antibody (Cat# AAA30567)

Full Name

ESRRA Polyclonal Antibody

Gene Names

ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Niban, Polyclonal Antibody (Cat# AAA31335)

Full Name

Niban Antibody

Gene Names

FAM129A; GIG39; NIBAN; C1orf24

Reactivity

Human, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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WB (Western Blot) (WB Suggested Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2)

NR4A1, Polyclonal Antibody (Cat# AAA23400)

Full Name

NR4A1 antibody - N-terminal region

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Protein A purified

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody. Western blot was performed from the immunoprecipitate using EIF3H antibody at a dilition of 1:1000.)

EIF3H, Polyclonal Antibody (Cat# AAA28265)

Full Name

EIF3H Polyclonal Antibody

Gene Names

EIF3H; EIF3S3; eIF3-p40; eIF3-gamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

GLI1, Polyclonal Antibody (Cat# AAA30569)

Full Name

GLI1 Polyclonal Antibody

Gene Names

GLI1; GLI

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HepG2 cells using anti-MYH14 antibody (AAA19818).Overlay histogram showing HepG2 cells stained with AAA19818 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYH14 Antibody (AAA19818, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of MYH14 using anti-MYH14 antibody (AAA19818).MYH14 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U-87MG whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: rat lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (#AAA19818) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.)

MYH14, Polyclonal Antibody (Cat# AAA19818)

Full Name

Anti-MYH14 Antibody Picoband

Gene Names

MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-PCMT1 antibody (AAA19819).Overlay histogram showing K562 cells stained with AAA19819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCMT1 Antibody (AAA19819, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PCMT1 using anti-PCMT1 antibody (AAA19819).PCMT1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (#AAA19819) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 25-28 kDa.)

PCMT1, Polyclonal Antibody (Cat# AAA19819)

Full Name

Anti-PCMT1 Antibody Picoband

Gene Names

PCMT1; PIMT

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using UHRF2 antibody at dilution of 1:200 (40x lens).)

UHRF2, Polyclonal Antibody (Cat# AAA28267)

Full Name

UHRF2 Polyclonal Antibody

Gene Names

UHRF2; NIRF; URF2; RNF107; TDRD23

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody.)

ECH1, Polyclonal Antibody (Cat# AAA29803)

Full Name

ECH1 Polyclonal Antibody

Gene Names

ECH1; HPXEL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

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TXN, Polyclonal Antibody (Cat# AAA30571)

Full Name

TXN Polyclonal Antibody

Gene Names

TXN; TRX; TRDX; TRX1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IP (Immunoprecipitation) (MYEF2 was immunoprecipitated using: Lane A:0.5 mg SH-SY5Y Whole Cell Lysate 4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 64 kDa Observed band size :64 kDa)

MYEF2, Polyclonal Antibody (Cat# AAA27756)

Full Name

Anti-MYEF2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

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PHB, Polyclonal Antibody (Cat# AAA23420)

Full Name

PHB antibody - C-terminal region

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Anti-H2AFM/HIST1H2AB rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: RAW246.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)

H2AFM/HIST1H2AB, Polyclonal Antibody (Cat# AAA27772)

Full Name

Anti-H2AFM/HIST1H2AB Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Protein A & Antigen Affinity

Pricing

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KIF14, Polyclonal Antibody (Cat# AAA30588)

Full Name

KIF14 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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SOX10, Polyclonal Antibody (Cat# AAA23423)

Full Name

SOX10 Antibody - middle region

Gene Names

SOX10; DOM; WS4; PCWH; WS2E; WS4C

Reactivity

Reactivity: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

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BLOC1S3, Polyclonal Antibody (Cat# AAA29823)

Full Name

BLOC1S3 Polyclonal Antibody

Gene Names

BLOC1S3; RP; HPS8; BLOS3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

ULK1, Polyclonal Antibody (Cat# AAA31359)

Full Name

Phospho-ULK1 (Ser757)[Ser758] Antibody

Gene Names

ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (88%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

Apolipoprotein A-I, Polyclonal Antibody (Cat# AAA18816)

Full Name

Rabbit anti-Bovine Apolipoprotein A-I polyclonal Antibody

Reactivity

Bovine, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

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AIFM1/AIF/PDCD8, Polyclonal Antibody (Cat# AAA21377)

Full Name

AIFM1/AIF/PDCD8 Goat anti-Human Polyclonal Antibody

Gene Names

AIFM1; AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6

Reactivity

Human

Applications

Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

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IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)

HDAC2, Polyclonal Antibody (Cat# AAA28801)

Full Name

HDAC2 Antibody (C-term)

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

GDF-6, Polyclonal Antibody (Cat# AAA29313)

Full Name

GDF-6 Polyclonal Antibody

Gene Names

GDF6; KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; MCOP4; SCDO4; MCOPCB6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Pricing

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SRF, Polyclonal Antibody (Cat# AAA31361)

Full Name

Phospho-SRF (Ser103) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (89%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

LINE-1, Polyclonal Antibody (Cat# AAA14722)

Full Name

LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)

Gene Names

L1TD1; ECAT11; RP5-1155K23.3

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB)

Purity

Purified
Purified by selective precipitation and salt fractionation followed by extensive dialysis.

Pricing

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IHC (Immunohistochemistry) (IHC image of AAA27003 diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

DMD, Polyclonal Antibody (Cat# AAA27003)

Full Name

DMD Antibody

Gene Names

DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC)

Purity

>95%, Protein G purified

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

VE-Cadherin, Polyclonal Antibody (Cat# AAA29307)

Full Name

VE-Cadherin Polyclonal Antibody

Gene Names

CDH5; 7B4; CD144

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HSPB1 antibody.)

HSPB1, Polyclonal Antibody (Cat# AAA29819)

Full Name

HSPB1 Polyclonal Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

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p53, Polyclonal Antibody (Cat# AAA31355)

Full Name

Acetyl-p53 (Lys381) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Rabbit (90%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IHC (Immunohistchemistry) (PARK7/DJ-1 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

PARK7/DJ-1, Polyclonal Antibody (Cat# AAA21370)

Full Name

PARK7/DJ-1 Rabbit anti-Human Polyclonal Antibody

Gene Names

PARK7; DJ1; DJ-1; HEL-S-67p

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Protein G purified

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PCK1, Polyclonal Antibody (Cat# AAA23418)

Full Name

PCK1 antibody - middle region

Gene Names

PCK1; PCKDC; PEPCK1; PEPCKC; PEPCK-C

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence staining of KLH-SMCCA1240+KLH-SMCCA2123 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1240+KLH-SMCCA2123 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)