Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistchemistry) (CDH1/E Cadherin Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CDH1/E Cadherin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CDH1/E Cadherin Antibody-Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CDH1/E Cadherin Antibody-Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate using 5ug of Anti-E Cadherin Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HEK293F whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-E Cadherin Antibody.)

WB (Western Blot) (CDH1/E Cadherin Antibody-Western blot analysis of E Cadherin expression in HEK293T (A); PC12 (B); A431 (C); MCF7 (D); C2C12 (E) whole cell lysates.)

ICC (Immunocytochemistry) (CDH1/E Cadherin Antibody-Immunofluorescent analysis of E Cadherin staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

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CDH1/E Cadherin, Polyclonal Antibody (Cat# AAA21351)

Full Name

CDH1/E Cadherin Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names

CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324

Reactivity

Zebrafish, Pig, Rat, Rabbit, Dog, Bovine, Human, Monkey

Applications

Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Immunoaffinity purified

Pricing

$650/0.05 mL $1,255/2x0.05 mL $1,825/3x0.05 mL $2,365/4x0.05 mL $2,680/5x0.05 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (At 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from A549, Hela and 3T3 cells using Twist2 Antibody)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, using Twist2 Antibody. The lane on the left was treated with blocking peptide.)

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Twist2, Polyclonal Antibody (Cat# AAA31341)

Full Name

Twist2 Antibody

Gene Names

TWIST2; AMS; FFDD3; BBRSAY; DERMO1; SETLSS; bHLHa39

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (92%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of MCF-7 cells using AAA18542 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA18542 at dilution of 1:100)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human prostate cancer using AAA18542 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using AAA18542 at dilution 1:50)

WB (Western Blot) (Western BlotPositive WB detected in:Hela whole cell lysate,A549 whole cell lysate,HEK293 whole cell lysate,HepG2 whole cell lysate,Jurkat whole cell lysate,K562 whole cell lysateAll lanes: GAPDH antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western BlotPositive WB detected in: hepG2 cell,mouse brain,mouse skeletal muscleAll lanes: GAPDH antibody at 2.6ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western blotAll lanes: GAPDH antibody at 2ug/mlLane 1: Hela whole cell lysateLane 2: 293T whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Mouse kidney tissueLane 5: Rat muscle tissueLane 6: Rat lung tissueLane 7: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

WB (Western Blot) (Western blotAll lanes: Glyceraldehyde-3-phosphate dehydrogenase antibody at 2ug/mlLane 1: Hale whole cell lysateLane 2: A549 whole cell lysateLane 3: HepG2 whole cell lysateLane 4: K562 whole cell lysateLane 5: Jurkats whole cell lysateLane 6: MDA-MB-231 whole cell lysateLane 7: LO2 whole cell lysateLane 8: U251 whole cell lysateLane 9: MCF-7 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 37,32 kDaObserved band size: 37 kDa)

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Glyceraldehyde-3-phosphate dehydrogenase, Polyclonal Antibody (Cat# AAA18542)

Full Name

Rabbit anti-human Glyceraldehyde-3-phosphate dehydrogenase polyclonal Antibody

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Rab4/RAB4A using anti-Rab4/RAB4A antibody (AAA19822).Rab4/RAB4A was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Rab4/RAB4A Antibody (AAA19822) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab4/RAB4A antigen affinity purified polyclonal antibody (#AAA19822) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rab4/RAB4A at approximately 24 kDa. The expected band size for Rab4/RAB4A is at 24 kDa.)

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Rab4/RAB4A, Polyclonal Antibody (Cat# AAA19822)

Full Name

Anti-Rab4/RAB4A Antibody Picoband

Gene Names

RAB4A; RAB4; HRES1; HRES-1; HRES-1/RAB4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-ATOH1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Placenta)

WB (Western Blot) (Host: RabbitTarget Name: ATOH1Sample Type: PlacentaLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/laneGel Concentration: 0.12%)

WB (Western Blot) (Host: RabbitTarget Name: ATOH1Sample Tissue: Human Jurkat Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ATOH1Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (mouse cochlea)

IHC (Immunohistochemistry) (Immunohistochemistry with Spleen cell lysate tissue)

IHC (Immunohistochemistry) (Immunohistochemistry with Spleen cell lysate tissue)

IHC (Immunohistochemistry) (Immunohistochemistry with Liver cell lysate tissue)

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ATOH1, Polyclonal Antibody (Cat# AAA23406)

Full Name

ATOH1 antibody - middle region

Gene Names

ATOH1; ATH1; HATH1; MATH-1; bHLHa14

Reactivity

Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

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IL-6Ralpha, Polyclonal Antibody (Cat# AAA29294)

Full Name

IL-6Ralpha Polyclonal Antibody

Gene Names

IL6R; IL6Q; gp80; CD126; IL6RA; IL6RQ; IL-6RA; IL-6R-1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus using KRT14 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat lung using KRT14 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse skin using KRT14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Human skin using KRT14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat skin using KRT14 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KRT14 antibody at 1:1000 dilution.)

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KRT14, Polyclonal Antibody (Cat# AAA30574)

Full Name

KRT14 Polyclonal Antibody

Gene Names

KRT14; K14; NFJ; CK14; EBS3; EBS4

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of GAPDH Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

IF (Immunofluorescence) (GAPDH Antibody (N-term) confocal immunofluorescent analysis with Hela cell. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (Western blot analysis of GAPDH Antibody (N-term) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GAPDH antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of lysates from Hela,HUVEC cell line (from left to right),using GAPDH Antibody (N-term). AAA28776 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from A375 cell line, using GAPDH Antibody (N-term). AAA28776 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

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GAPDH, Polyclonal Antibody (Cat# AAA28776)

Full Name

GAPDH Antibody (N-term)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Reactivity

Human

Applications

Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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WB (Western Blot) (Western blot analysis of Catenin 1 Antibody expression in MCF-7 cells lysates.The lane on the left is treated with the antigen-specific peptide..The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistchemistry) (Catenin alpha1 Antibody for IHC in human gallbladder tissue.)

WB (Western Blot) (Western blot analysis of extracts from mouse brain, using Catenin alpha1 Antibody.)

WB (Western Blot) (Western blot analysis of Catenin alpha1 expression in mouse muscle tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of Catenin alpha1 expression in various lysates)

WB (Western Blot) (Western blot analysis of Catenin alpha1 expression in S3/rat liver tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

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Catenin alpha1, Polyclonal Antibody (Cat# AAA31080)

Full Name

Catenin alpha1 Antibody

Gene Names

CTNNA1; MDPT2; CAP102

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$330/0.1 mL $385/0.2 mL $740/2x0.2 mL $1,070/3x0.2 mL $1,385/4x0.2 mL $1,480/5x0.2 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U251 cells using anti-RPS5 antibody (AAA19817).Overlay histogram showing U251 cells stained with AAA19817 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS5 Antibody (AAA19817, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of RPS5 using anti-RPS5 antibody (AAA19817).RPS5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 7. IF analysis of RPS5 using anti-RPS5 antibody (AAA19817).RPS5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 6. IF analysis of RPS5 using anti-RPS5 antibody (AAA19817).RPS5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RPS5 Antibody (AAA19817) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse ovary tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS5 antigen affinity purified polyclonal antibody (#AAA19817) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPS5 at approximately 23 kDa. The expected band size for RPS5 is at 23 kDa.)

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RPS5, Polyclonal Antibody (Cat# AAA19817)

Full Name

Anti-RPS5 Antibody Picoband

Gene Names

RPS5; S5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (CANX/Calnexin Antibody-Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (CANX/Calnexin Antibody-Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CANX/Calnexin Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CANX/Calnexin Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (CANX/Calnexin Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (CANX/Calnexin Antibody-Western blot analysis of Calnexin expression in MCF7 (A); HeLa (B); MDAMB231 (C) whole cell lysates.)

ICC (Immunocytochemistry) (CANX/Calnexin Antibody-Immunofluorescent analysis of Calnexin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

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CANX/Calnexin, Polyclonal Antibody (Cat# AAA21353)

Full Name

CANX/Calnexin Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names

CANX; CNX; P90; IP90

Reactivity

Mouse, Dog, Bovine, Rat, Human

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP), Western Blot (WB)

Purity

Immunoaffinity purified

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IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse Inflammatory lung using Ly6g Polyclonal Antibody at dilution of 1:200.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded mouse Inflammatory lung using Ly6g Polyclonal Antibody at dilution of 1:200.)

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Ly6g, Polyclonal Antibody (Cat# AAA22121)

Full Name

Ly6g Polyclonal Antibody

Reactivity

Mouse

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$260/0.06 mL $350/0.12 mL $500/0.2 mL $965/2x0.2 mL $1,400/3x0.2 mL $1,810/4x0.2 mL $2,180/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-HNF4A antibody Titration: 1 ug/mLSample Type: Human Liver)

WB (Western Blot) (WB Suggested Anti-HNF4A Antibody Titration: 0.2-1 ug/ml.A: - blocking peptide.B: + blocking peptide.)

WB (Western Blot) (Host: RatTarget Name: HNF4ASample Tissue: Rat Skeletal MuscleAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HNF4ASample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HNF4ASample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: HNF4ASample Tissue: Human Lung TumorAntibody Dilution: 1.0ug/ml)

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HNF4A, Polyclonal Antibody (Cat# AAA23401)

Full Name

HNF4A Antibody - N-terminal region

Gene Names

HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

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GCP5, Polyclonal Antibody (Cat# AAA29029)

Full Name

GCP5 Polyclonal Antibody

Gene Names

TUBGCP5; GCP5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

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TRPC3, Polyclonal Antibody (Cat# AAA29285)

Full Name

TRPC3 Polyclonal Antibody

Gene Names

TRPC3; TRP3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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GNL3L, Polyclonal Antibody (Cat# AAA29031)

Full Name

GNL3L Polyclonal Antibody

Gene Names

GNL3L; RP11-353K22.1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ESRRA antibody at 1:1000 dilution.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using ESRRA antibody at dilution of 1:200 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterus using ESRRA antibody at dilution of 1:200 .)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ESRRA antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

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ESRRA, Polyclonal Antibody (Cat# AAA30567)

Full Name

ESRRA Polyclonal Antibody

Gene Names

ESRRA; ERR1; ERRa; ESRL1; NR3B1; ERRalpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from A431 and DU145 cells using Niban Antibody)

WB (Western Blot) (Western blot analysis of extracts from Rat brain, using Niban Antibody. The lane on the left was treated with blocking peptide.)

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Niban, Polyclonal Antibody (Cat# AAA31335)

Full Name

Niban Antibody

Gene Names

FAM129A; GIG39; NIBAN; C1orf24

Reactivity

Human, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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WB (Western Blot) (WB Suggested Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2)

WB (Western Blot) (Host: RabbitTarget Name: NR4A1Sample Tissue: Human HelaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: NR4A1Sample Tissue: Human 786-0 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-NR4A1 AntibodyParaffin Embedded Tissue: Human StomachCellular Data: Mucous CellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-NR4A1 AntibodyParaffin Embedded Tissue: Human LiverCellular Data: HepatocytesAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Human Testis)

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NR4A1, Polyclonal Antibody (Cat# AAA23400)

Full Name

NR4A1 antibody - N-terminal region

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Protein A purified

Pricing

$445/0.1 mL $855/2x0.1 mL $1,245/3x0.1 mL $1,610/4x0.1 mL $1,800/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug EIF3H antibody. Western blot was performed from the immunoprecipitate using EIF3H antibody at a dilition of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using EIF3H Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EIF3H Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using EIF3H Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using EIF3H Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using EIF3H antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

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EIF3H, Polyclonal Antibody (Cat# AAA28265)

Full Name

EIF3H Polyclonal Antibody

Gene Names

EIF3H; EIF3S3; eIF3-p40; eIF3-gamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Affinity Purification

Pricing

$235/0.02 mL $400/0.1 mL $770/2x0.1 mL $1,115/3x0.1 mL $1,440/4x0.1 mL $1,530/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using GLI1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using GLI1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using GLI1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human mammary cancer using GLI1 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using GLI1 antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GLI1 antibody at 1:1000 dilution.)

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GLI1, Polyclonal Antibody (Cat# AAA30569)

Full Name

GLI1 Polyclonal Antibody

Gene Names

GLI1; GLI

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HepG2 cells using anti-MYH14 antibody (AAA19818).Overlay histogram showing HepG2 cells stained with AAA19818 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYH14 Antibody (AAA19818, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of MYH14 using anti-MYH14 antibody (AAA19818).MYH14 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MYH14 Antibody (AAA19818) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U-87MG whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: rat lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH14 antigen affinity purified polyclonal antibody (#AAA19818) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MYH14 at approximately 250 kDa. The expected band size for MYH14 is at 228 kDa.)

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MYH14, Polyclonal Antibody (Cat# AAA19818)

Full Name

Anti-MYH14 Antibody Picoband

Gene Names

MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-PCMT1 antibody (AAA19819).Overlay histogram showing K562 cells stained with AAA19819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCMT1 Antibody (AAA19819, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-PCMT1 antibody (AAA19819).Overlay histogram showing HepG2 cells stained with AAA19819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PCMT1 Antibody (AAA19819, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of PCMT1 using anti-PCMT1 antibody (AAA19819).PCMT1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PCMT1 Antibody (AAA19819) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: rat brain tissue lysates,Lane 3: rat liver tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse testis tissue lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (#AAA19819) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 25-28 kDa.)

WB (Western Blot) (Figure 1. Western blot analysis of PCMT1 using anti-PCMT1 antibody (AAA19819).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Raji whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human K562 whole cell lysates,Lane 6: human MCF-7 whole cell lysates,Lane 7: human Caco-2 whole cell lysates,Lane 8: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCMT1 antigen affinity purified polyclonal antibody (#AAA19819) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( 25-28 kDa.)

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PCMT1, Polyclonal Antibody (Cat# AAA19819)

Full Name

Anti-PCMT1 Antibody Picoband

Gene Names

PCMT1; PIMT

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using UHRF2 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using UHRF2 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using UHRF2 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using UHRF2 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using UHRF2 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using UHRF2 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat Intestine using UHRF2 antibody at dilution of 1:200 (40x lens).)

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UHRF2, Polyclonal Antibody (Cat# AAA28267)

Full Name

UHRF2 Polyclonal Antibody

Gene Names

UHRF2; NIRF; URF2; RNF107; TDRD23

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purification

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using ECH1 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human placenta using ECH1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ECH1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using ECH1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using ECH1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using ECH1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ECH1 Antibody.)

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ECH1, Polyclonal Antibody (Cat# AAA29803)

Full Name

ECH1 Polyclonal Antibody

Gene Names

ECH1; HPXEL

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using TXN antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using TXN antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using TXN antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using TXN antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using TXN antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using TXN antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using TXN antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using TXN antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using TXN antibody at dilution of 1:100 .)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TXN antibody at 1:1000 dilution.)

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TXN, Polyclonal Antibody (Cat# AAA30571)

Full Name

TXN Polyclonal Antibody

Gene Names

TXN; TRX; TRDX; TRX1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IP (Immunoprecipitation) (MYEF2 was immunoprecipitated using: Lane A:0.5 mg SH-SY5Y Whole Cell Lysate 4 uL anti-MYEF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MYEF2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 64 kDa Observed band size :64 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of MYEF2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MYEF2 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining of human MYEF2 in human kidney with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human MYEF2 in human brain with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human MYEF2 in human testis with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-MYEF2 rabbit polyclonal antibody at 1:500 dilution Lane A: SH-SY5Y Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:64 kDa Observed band size:70 kDa)

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MYEF2, Polyclonal Antibody (Cat# AAA27756)

Full Name

Anti-MYEF2 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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WB (Western Blot) (WB Suggested Anti-PHB Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat cell lysatePHB is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: RabbitTarget Name: PHBSample Type: Daudi Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PHBSample Type: 721_b Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PHBSample Type: 293 Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PHBSample Tissue: Human 293TAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (PHB antibody - C-terminal region Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasm of pneumocytesPrimary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (PHB antibody - C-terminal region Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue Observed Staining: Cytoplasm in hepatocytesPrimary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (PHB antibody - C-terminal region Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasm in Human Bronchial Epithelial TissuePrimary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec)

IHC (Immunohistochemistry) (Human Stomach)

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PHB, Polyclonal Antibody (Cat# AAA23420)

Full Name

PHB antibody - C-terminal region

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Anti-H2AFM/HIST1H2AB rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: RAW246.7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size:14 kDaObserved band size:14 kDa)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human HIST1H2AB in human colon with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human HIST1H2AB in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of Mouse HIST1H2AB in Mouse large intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of Mouse HIST1H2AB in Mouse liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IF (Immunofluorescence) (Immunofluorescence staining of KLH-SMCCA1245 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1245 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)

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H2AFM/HIST1H2AB, Polyclonal Antibody (Cat# AAA27772)

Full Name

Anti-H2AFM/HIST1H2AB Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using KIF14 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using KIF14 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KIF14 at 1:1000 dilution.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using KIF14 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using KIF14 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using KIF14 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using KIF14 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using KIF14 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using KIF14 at dilution of 1:100 (40x lens).)

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KIF14, Polyclonal Antibody (Cat# AAA30588)

Full Name

KIF14 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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WB (Western Blot) (Western Blot (WB)Host: RabbitTarget: SOX10Positive control (+): Rat brain (R-BR)Negative control (-): Mouse intestine (M-IN) Antibody concentration: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-SOX10Antibody Titration: .25ug/mlELISA Titer: 1:62500Positive Control: HepG2 cell lysate)

WB (Western Blot) (SOX10 antibody - middle region validated by WB using Hek 293 Whole Cell Lysate at 1:4,000.)

WB (Western Blot) (Host: RabbitTarget Name: SOX10Sample Type: Jurkat cell lysatesLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 2.5ug/mlPeptide Concentration: 4.0ug/mlLysate Quantity: 25ug/laneGel Concentration: 12%)

WB (Western Blot) (Host: RabbitTarget Name: SOX10Sample Type: HepG2 Whole Cell lysatesAntibody Dilution: 0.5ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: SOX10Sample Tissue: Human MCF7 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Immunohistochemistry with Skin tissue at an antibody concentration of 4-8ug/ml using anti-SOX10 antibody)

IHC (Immunohistochemistry) (Immunohistochemistry with Kidney lysate tissue at an antibody concentration of 4-8ug/ml using anti-SOX10 antibody)

IHC (Immunohistochemistry) (Sample Type: human optic nerves and spinal cordA/[IHC-PZ] (optimal processing)human optic nerve (short post mortem interval) fixed by immersion in zinc-based fixative (BD Pharmingen 552658), processed to minimize antigen loss (shortened protocol, reduced exposure to high temperature); embedded in paraffin wax; sectioned at 3 micronsA2/[IHC-PZ + FF]As above for initial fixation then post-fixed in 10% buffered formalin for 5 daysB/ [IHC-P]human optic nerve and spinal cord fixed in 10% buffered formalin (relatively short post mortem interval and fixation duration); standard processing; embedded in paraffin wax; sectioned at 3 micronsControlsNegative: omission of primary.Positive: Olig2 reactivity was in comparison with antibody from Prof Charles Stiles/ Dr John Alberta (DF308; N terminal Olig2) used at 1:10000 (IHC-PZ); 1:4000 (IHC-P))

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SOX10, Polyclonal Antibody (Cat# AAA23423)

Full Name

SOX10 Antibody - middle region

Gene Names

SOX10; DOM; WS4; PCWH; WS2E; WS4C

Reactivity

Reactivity: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using BLOC1S3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using BLOC1S3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using BLOC1S3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using BLOC1S3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using BLOC1S3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using BLOC1S3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using BLOC1S3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using BLOC1S3 antibody.)

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BLOC1S3, Polyclonal Antibody (Cat# AAA29823)

Full Name

BLOC1S3 Polyclonal Antibody

Gene Names

BLOC1S3; RP; HPS8; BLOS3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from PC12 cells, using Phospho-ULK1 (Ser757) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

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ULK1, Polyclonal Antibody (Cat# AAA31359)

Full Name

Phospho-ULK1 (Ser757)[Ser758] Antibody

Gene Names

ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (88%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

WB (Western Blot) (Western blotAll lanes: APOA1antibody at 2ug/mlLane 1:mouse liver tissueLane 2:mouse lung tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 30kDaObserved band size: 30kDa)

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Apolipoprotein A-I, Polyclonal Antibody (Cat# AAA18816)

Full Name

Rabbit anti-Bovine Apolipoprotein A-I polyclonal Antibody

Reactivity

Bovine, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Caprylic Acid Ammonium Sulfate Precipitation Purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

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IHC (Immunohistchemistry) (AIFM1/AIF/PDCD8 Antibody-Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (AIFM1/AIF/PDCD8 Antibody-Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (AIFM1/AIF/PDCD8 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.01ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.01ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer) Detected by chemiluminescence.)

WB (Western Blot) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody (0.03ug/ml) staining of Mouse (A) and Rat (B) Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

ICC (Immunocytochemistry) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

ICC (Immunocytochemistry) (AIFM1/AIF/PDCD8 Antibody-AIFM1/AIF/PDCD8 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

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AIFM1/AIF/PDCD8, Polyclonal Antibody (Cat# AAA21377)

Full Name

AIFM1/AIF/PDCD8 Goat anti-Human Polyclonal Antibody

Gene Names

AIFM1; AIF; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6

Reactivity

Human

Applications

Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

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IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with HDAC2 Antibody (C-term) .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). hHDAC2 immunoreactivity is localized to Nucleus significantly.)

IHC (Immunohistochemistry-Paraffin) (HDAC2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of HDAC2 Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)

WB (Western Blot) (Western blot analysis of anti-HDAC2 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of anti-HDAC2 Antibody (C-term) in 293 cell line lysates (35ug/lane). HDAC2(arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of HDAC2 (arrow) using rabbit polyclonal HDAC2 Antibody (C-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HDAC2 gene (Lane 2) (Origene Technologies).)

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HDAC2, Polyclonal Antibody (Cat# AAA28801)

Full Name

HDAC2 Antibody (C-term)

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), ELISA (EIA)

Pricing

$250/0.08 mL $480/0.4 mL $925/2x0.4 mL $1,345/3x0.4 mL $1,740/4x0.4 mL $1,975/5x0.4 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

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GDF-6, Polyclonal Antibody (Cat# AAA29313)

Full Name

GDF-6 Polyclonal Antibody

Gene Names

GDF6; KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; MCOP4; SCDO4; MCOPCB6

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 TPA-treated, using Phospho-SRF (Ser103) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

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SRF, Polyclonal Antibody (Cat# AAA31361)

Full Name

Phospho-SRF (Ser103) Antibody

Gene Names

SRF; MCM1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (89%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

WB (Western Blot) (Western Blot using AAA14722 shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70kD corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).)

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LINE-1, Polyclonal Antibody (Cat# AAA14722)

Full Name

LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)

Gene Names

L1TD1; ECAT11; RP5-1155K23.3

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB)

Purity

Purified
Purified by selective precipitation and salt fractionation followed by extensive dialysis.

Pricing

$1,120/0.1 mg $2,170/2x0.1 mg $3,165/3x0.1 mg $4,105/4x0.1 mg $4,800/5x0.1 mg

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IHC (Immunohistochemistry) (IHC image of AAA27003 diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA27003 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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DMD, Polyclonal Antibody (Cat# AAA27003)

Full Name

DMD Antibody

Gene Names

DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC)

Purity

>95%, Protein G purified

Pricing

$240/0.05 mg $340/0.1 mg $650/2x0.1 mg $945/3x0.1 mg $1,220/4x0.1 mg $1,445/5x0.1 mg

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

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VE-Cadherin, Polyclonal Antibody (Cat# AAA29307)

Full Name

VE-Cadherin Polyclonal Antibody

Gene Names

CDH5; 7B4; CD144

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HSPB1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using HSPB1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using HSPB1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using HSPB1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using HSPB1 antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and HSPB1 knockout (KO) HeLa cells, using HSPB1 antibody.)

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HSPB1, Polyclonal Antibody (Cat# AAA29819)

Full Name

HSPB1 Polyclonal Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Acetyl-p53 (K381) in lysates of MDA-MB-435 cells(TSA 1M, 18 hr), using Acetyl-p53 (K381) Antibody(AF4364).)

WB (Western Blot) (Western blot analysis of extracts from MDA-MB-435 cells(TSA 1M, 18 hr), using Acetyl-p53 (Lys381) Antibody.Lane1 was treated with Ac-blocking peptide.Lane2 was treated with Non-Ac-blocking peptide.)

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p53, Polyclonal Antibody (Cat# AAA31355)

Full Name

Acetyl-p53 (Lys381) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Rabbit (90%), Dog (100%)

Applications

Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$315/0.05 mL $385/0.1 mL $455/0.2 mL $875/2x0.2 mL $1,270/3x0.2 mL $1,645/4x0.2 mL $1,810/5x0.2 mL

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IHC (Immunohistchemistry) (PARK7/DJ-1 Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (PARK7/DJ-1 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (PARK7/DJ-1 Antibody-Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (PARK7/DJ-1 Antibody-Western Blot Positive WB detected in: HEK293 whoel cell lysate All lanes: PARK7 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa)

Application Data (PARK7/DJ-1 Antibody-Western blot. All lanes: PARK7 antibody at 5 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: SH-SY5Y whole cell lysate. Lane 5: Mouse brain tissue. Lane 6: Mouse liver tissue. Lane 7: Mouse heart tissue. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 20 kDa. Observed band size: 20 kDa.)

ICC (Immunocytochemistry) (PARK7/DJ-1 Antibody)

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PARK7/DJ-1, Polyclonal Antibody (Cat# AAA21370)

Full Name

PARK7/DJ-1 Rabbit anti-Human Polyclonal Antibody

Gene Names

PARK7; DJ1; DJ-1; HEL-S-67p

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)

Purity

Protein G purified

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WB (Western Blot) (WB Suggested Anti-PCK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate)

WB (Western Blot) (WB Suggested Anti-PCK1 AntibodyPositive Control: Lane 1: 80ug pig serum proteinPrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-HRPSecondry Antibody Dilution : 1:500Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna)

WB (Western Blot) (WB Suggested Anti-PCK1 AntibodyPositive Control: Lane 1: 80ug pig serumPrimary Antibody Dilution : 1:1000Secondary Antibody : Anti-rabbit-HRPSecondry Antibody Dilution : 1:1000Submitted by: Martina Ondrovics, University of Veterinary Medicine Vienna)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Type: MCF7Antibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Type: Human Fetal LiverAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: PCK1Sample Tissue: Human MCF7 Whole CellAntibody Dilution: 1ug/ml)

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PCK1, Polyclonal Antibody (Cat# AAA23418)

Full Name

PCK1 antibody - middle region

Gene Names

PCK1; PCKDC; PEPCK1; PEPCKC; PEPCK-C

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of KLH-SMCCA1240+KLH-SMCCA2123 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human KLH-SMCCA1240+KLH-SMCCA2123 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IHC (Immunohistchemistry-Paraffin) (Immunochemical staining of rat D1 in rat kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of rat D1 in rat liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of Mouse HSPD1 in Mouse kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human HSPD1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human HSPD1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-HSPD1 rabbit polyclonal antibody at 1:500 dilutionLane A: NIH-3T3 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:60 kDa)

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HSP60, Polyclonal Antibody (Cat# AAA27770)

Full Name

Anti-HSP60 Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot (WB), ELISA (EIA),Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Protein A & Antigen Affinity

Pricing

$375/0.1 mL $720/2x0.1 mL $1,045/3x0.1 mL $1,350/4x0.1 mL $1,450/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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P-Selectin, Polyclonal Antibody (Cat# AAA29306)

Full Name

P-Selectin Polyclonal Antibody

Gene Names

SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse oophoroma cells using ZP2 at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ZP2 at 1:1000 dilution.)

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ZP2, Polyclonal Antibody (Cat# AAA30586)

Full Name

ZP2 Rabbit Polyclonal Antibody

Gene Names

ZP2; ZPA; Zp-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$330/0.05 mL $420/0.1 mL $805/2x0.1 mL $1,170/3x0.1 mL $1,515/4x0.1 mL $1,670/5x0.1 mL

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IHC (Immunohistochemistry) (MDH/MDH2 Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistchemistry) (MDH/MDH2 Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (MDH/MDH2 Antibody-Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot) (MDH/MDH2 Antibody-Antibody (0.1ug/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) (MDH/MDH2 Antibody-Antibody (0.01ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) (MDH/MDH2 Antibody-Goat anti-MDH2 Antibody (0.1ug/ml) staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

WB (Western Blot) (MDH/MDH2 Antibody-Goat anti-MDH2 Antibody (0.01ug/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescencence.)

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MDH/MDH2, Polyclonal Antibody (Cat# AAA21368)

Full Name

MDH/MDH2 Goat anti-Human Polyclonal (Internal Region) Antibody

Gene Names

MDH2; MDH; MOR1; M-MDH; MGC:3559

Reactivity

Mouse, Rat, Pig, Human

Applications

Immunohistochemistry-Paraffin (IHC-P), Peptide-ELISA (EIA), Western Blot (WB)

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

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Application Data (Human lung cell line)

Application Data (Human lung epithelial cell)

WB (Western Blot) (Host: RabbitTarget Name: KLF2Sample Type: Hela Whole cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/laneGel Concentration: 0.12%)

IHC (Immunohistchemistry) (Immunohistochemistry with Human lung.)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Colon, myenteric plexus.)

WB (Western Blot) (WB Suggested Anti-KLF2 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 721_B cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: KLF2Sample Tissue: Human MCF7 Whole CellAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Immunohistochemistry with Human brain, cerebellum)

ChIP (Chromatin Immunoprecipitation) (Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.)

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KLF2, Polyclonal Antibody (Cat# AAA23416)

Full Name

KLF2 antibody - middle region

Gene Names

KLF2; LKLF

Reactivity

Human
Predicted: Guinea Pig, Human, Mouse, Pig, Rat, Zebrafish

Applications

Chromatin IP (ChIP), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Affinity Purified

Pricing

$495/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.