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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 450-500 of 3176 product results
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of RT4 cells using anti-NUMA/NUMA1 antibody (AAA19766).Overlay histogram showing RT4 cells stained with AAA19766 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMA/NUMA1 Antibody (AAA19766, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NUMA/NUMA1, Polyclonal Antibody (Cat# AAA19766)
Full Name
Anti-NUMA/NUMA1 Antibody Picoband
Gene Names
NUMA1; NUMA
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Pam212 cells, untreated or sulindac sulfone- treated (to induce apoptosis), using AAA14725 Results were similar to those obtained by analyzing DNA content.)
Caspase 3, Cleaved, Polyclonal Antibody (Cat# AAA14725)
Full Name
Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Reactivity
Human, Monkey, Mouse, Rat
Applications
EL/EIA, WB, IHC, FC/FACS, IF
Purity
Affinity Purified
Purified by Protein A and immunoaffinity chromatography.
Purified by Protein A and immunoaffinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Smad2, Polyclonal Antibody (Cat# AAA29117)
Full Name
Smad2 Polyclonal Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
WB (Western Blot)
(Detection of mouse PCNA by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PCNA antibody (AAA23771 lot 2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
PCNA, Polyclonal Antibody (Cat# AAA23771)
Full Name
Rabbit anti-PCNA Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
WB, IP, IHC, IHC-IF
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Liver Tissue.)
Syndecan 1, Polyclonal Antibody (Cat# AAA20851)
Full Name
Polyclonal Antibody to Syndecan 1 (SDC1)
Gene Names
SDC1; SDC; CD138; SYND1; syndecan
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Renal cancer Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human GaA AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Glucosidase Alpha, Acid (GaA), Polyclonal Antibody (Cat# AAA20106)
Full Name
Polyclonal Antibody to Glucosidase Alpha, Acid (GaA)
Gene Names
GAA; LYAG
Reactivity
Human
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of MCF-7 cells using anti-PDF antibody (AAA19730).Overlay histogram showing MCF-7 cells stained with AAA19730 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDF Antibody (AAA19730, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDF, Polyclonal Antibody (Cat# AAA19730)
Full Name
Anti-PDF Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Anti-SOST Antibody (N-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SOST, Polyclonal Antibody (Cat# AAA28704)
Full Name
SOST Antibody (N-term)
Gene Names
SOST; CDD; VBCH; SOST1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Rat lung using LGALS3 Polycloanl Antibody at dilution of 1:200)
Galectin 3, Polyclonal Antibody (Cat# AAA22146)
Full Name
Galectin 3 Polyclonal Antibody
Gene Names
Lgals3; GBP; L-34; gal3; Mac-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Antigen Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-RCAN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle)
RCAN1, Polyclonal Antibody (Cat# AAA23463)
Full Name
RCAN1 antibody - middle region
Gene Names
RCAN1; CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of K562 cells using anti-HNRNPH3 antibody (AAA19338).Overlay histogram showing K562 cells stained with AAA19338 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH3 Antibody (AAA19338, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HNRNPH3, Polyclonal Antibody (Cat# AAA19338)
Full Name
Anti-HNRNPH3 Antibody
Gene Names
HNRNPH3; 2H9; HNRPH3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
Application Data
(BALB/c mouse splenocytes were stained with Goat F(ab)2 Anti-Mouse Kappa-BIOT and Rat Anti-Mouse CD19-PE followed by Streptavidin-FITC.)
Goat F(ab')2 Anti-Mouse kappa (kappa chain specific), Polyclonal Secondary Antibody (Cat# AAA14899)
Full Name
Goat F(ab')2 Anti-Mouse Kappa-LE/AF
Reactivity
Mouse
Applications
EIA
Purity
Gel filtration chromatography of pepsin digested antibody
Pricing
IF (Immunofluorescence)
(Figure 9. IF analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody (AAA19723).E-Cadherin/Cdh1 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-E-Cadherin/Cdh1 Antibody (AAA19723) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
E-cadherin/Cdh1, Polyclonal Antibody (Cat# AAA19723)
Full Name
Anti-E-cadherin/Cdh1 Antibody Picoband
Gene Names
Cdh1; Um; UVO; Ecad; E-cad; L-CAM; AA960649
Reactivity
Mouse, Rat
Applications
EIA, FC/FACS, IF, IHC, WB
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of THP-1 cells using anti-IKZF3 antibody (AAA19753).Overlay histogram showing THP-1 cells stained with AAA19753 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKZF3 Antibody (AAA19753, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IKZF3, Polyclonal Antibody (Cat# AAA19753)
Full Name
Anti-IKZF3 Antibody Picoband
Gene Names
IKZF3; AIO; AIOLOS; ZNFN1A3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
NFAT2, Polyclonal Antibody (Cat# AAA31381)
Full Name
Phospho-NFAT2 (Ser294) Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Predicted Reactivity: Bovine (85%), Rabbit (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U2OS cells using anti-SAP130 antibody (AAA19584).Overlay histogram showing U2OS cells stained with AAA19584 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAP130 Antibody (AAA19584, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10 ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SAP130, Polyclonal Antibody (Cat# AAA19584)
Full Name
Anti-SAP130 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot analysis of extracts from K562 cells, using AAT Polyclonal Antibody. Secondary antibody was diluted at 1:20000)
AAT, Polyclonal Antibody (Cat# AAA30751)
Full Name
AAT Rabbit Polyclonal Antibody
Gene Names
SERPINA1; PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-P, IF, paraffin section, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
((4ug/ml) staining of paraffin embedded Human Lymph Node. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
ZCCHC11/PAPD3, Polyclonal Antibody (Cat# AAA13653)
Full Name
Goat anti-ZCCHC11/PAPD3 Antibody
Gene Names
ZCCHC11; TUT4; PAPD3
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
EIA, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: GLI2Sample Type: JurkatLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 2.5ug/mLPeptide Concentration: 4.0ug/mLLysate Quantity: 25ug/laneGel Concentration: 6%-18%)
GLI2, Polyclonal Antibody (Cat# AAA23395)
Full Name
GLI2 antibody - middle region
Gene Names
GLI2; CJS; HPE9; PHS2; THP1; THP2
Reactivity
Human
Applications
IHC, WB
Purity
Affinity Purified
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
H2B, Polyclonal Antibody (Cat# AAA31349)
Full Name
Acetyl-H2B (Lys16) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
CD235a, Polyclonal Antibody (Cat# AAA29310)
Full Name
CD235a Polyclonal Antibody
Gene Names
GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
Reactivity
Human
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody.)
BAF60a, Polyclonal Antibody (Cat# AAA29858)
Full Name
BAF60a Polyclonal Antibody
Gene Names
Smarcd1; Baf60a; D15Kz1; AA407987
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of SH-SY5Y cells using anti-PLD5 antibody (AAA19992).Overlay histogram showing SH-SY5Y cells stained with AAA19992 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PLD5 Antibody (AAA19992, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
PLD5, Polyclonal Antibody (Cat# AAA19992)
Full Name
Anti-PLD5 Antibody Picoband
Gene Names
PLD5; PLDC
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB Suggested Anti-CENPQ Antibody Titration: 0.2-1 ug/mlPositive Control: Human Liver)
CENPQ, Polyclonal Antibody (Cat# AAA23575)
Full Name
CENPQ antibody - N-terminal region
Gene Names
CENPQ; CENP-Q; C6orf139
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Pricing
Application Data
(Li, X., Ma, N., Zhang, Y. et al. Circular RNA circNRIP1 promotes migration and invasion in cervical cancer by sponging miR-629-3p and regulating the PTP4A1/ERK1/2 pathway. Cell Death Dis 11, 399 (2020).)
ERK 1/2, Polyclonal Antibody (Cat# AAA30746)
Full Name
ERK 1/2 (Phospho-Thr202/Y204) Rabbit Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
IF, ICC, WB, IHC-P, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)
Nrf2, Polyclonal Antibody (Cat# AAA29225)
Full Name
Nrf2 Polyclonal Antibody
Gene Names
NFE2L2; NRF2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.)
CEP135, Polyclonal Antibody (Cat# AAA28992)
Full Name
CEP135 Polyclonal Antibody
Gene Names
CEP135; CEP4; MCPH8; KIAA0635
Reactivity
Human, Mouse
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of PC12 cells using EGFR antibody.)
EGFR, Polyclonal Antibody (Cat# AAA29798)
Full Name
EGFR Polyclonal Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)
C4a/b, Polyclonal Antibody (Cat# AAA29293)
Full Name
C4a/b Polyclonal Antibody
Gene Names
C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2
Reactivity
Human
Applications
WB, IHC-P
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, ELISA 1:10000-20000)
TMPO, Polyclonal Antibody (Cat# AAA29342)
Full Name
TMPO Polyclonal Antibody
Gene Names
TMPO; TP; LAP2; CMD1T; LEMD4; PRO0868
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
WB (Western Blot)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
p21, Polyclonal Antibody (Cat# AAA29087)
Full Name
p21 Polyclonal Antibody
Gene Names
CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
POLR2A, Polyclonal Antibody (Cat# AAA31440)
Full Name
Phospho-POLR2A (Ser1616/Ser2) Antibody
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Mouse Colon Tissue;Primary Ab: 30ug/ml Rabbit Anti-Mouse C4a AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Complement Component 4b (C4b), Polyclonal Antibody (Cat# AAA20120)
Full Name
Polyclonal Antibody to Complement Component 4b (C4b)
Gene Names
C4b; C4; Ss
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using CAMK1D antibody.)
CAMK1D, Polyclonal Antibody (Cat# AAA28183)
Full Name
CAMK1D
Gene Names
CAMK1D; CKLiK; CaM-K1; CaMKID
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
MOR-1, Polyclonal Antibody (Cat# AAA28956)
Full Name
MOR-1 (phospho Ser375) Polyclonal Antibody
Gene Names
OPRM1; MOP; MOR; LMOR; MOR1; OPRM; M-OR-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Lymphotactin, Polyclonal Antibody (Cat# AAA29212)
Full Name
Lymphotactin Polyclonal Antibody
Gene Names
XCL1; LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
Reactivity
Human
Applications
WB, IHC-P
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using RUVBL1 antibody. Blue: DAPI for nuclear staining.)
RUVBL1, Polyclonal Antibody (Cat# AAA28185)
Full Name
RUVBL1 Polyclonal Antibody
Gene Names
RUVBL1; RVB1; TIH1; ECP54; TIP49; INO80H; NMP238; PONTIN; TIP49A; Pontin52
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of TMEM41B in Rat LungImmunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMEM41B, Polyclonal Antibody (Cat# AAA11035)
Full Name
TMEM41B (CT) Antibody
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Applications
EIA, IF, WB
Purity
TMEM41B Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human breast cancer using RCC1 antibody at dilution of 1:100 (40x lens).)
RCC1, Polyclonal Antibody (Cat# AAA10777)
Full Name
RCC1 Polyclonal Antibody
Gene Names
RCC1; CHC1; RCC1-I; SNHG3-RCC1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using SERPINB1 Rabbit pAb (AAA22061) at dilution of 1:100 (40x lens).)
SERPINB1, Polyclonal Antibody (Cat# AAA22061)
Full Name
SERPINB1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Anti-Kv2.1 Picoband antibody, AAA11600-7.JPGIHC(F): Mouse Brain Tissue)
Kv2.1, Polyclonal Antibody (Cat# AAA11600)
Full Name
Anti-Kv2.1 Antibody
Gene Names
KCNB1; DRK1; KV2.1; EIEE26; h-DRK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western Blot (WB)Host: RabbitTarget: SOX10Positive control (+): Rat brain (R-BR)Negative control (-): Mouse intestine (M-IN) Antibody concentration: 1ug/ml)
SOX10, Polyclonal Antibody (Cat# AAA23423)
Full Name
SOX10 Antibody - middle region
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Reactivity: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K23 at dilution of 1:100. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.)
Acetyl-Histone H3-K23, Polyclonal Antibody (Cat# AAA28365)
Full Name
Acetyl-Histone H3-K23 Rabbit pAb
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot analysis of human muscle lysate (35ug protein in RIPAbuffer) using AAA24036 (0.01ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
COX4I1, Polyclonal Antibody (Cat# AAA24036)
Full Name
COX4I1, CT (Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial, Cytochrome C Oxidase Polypeptide IV, Cytochrome C Oxidase Subunit IV Isoform 1, COX IV-1, COX IV-1)
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of THP-1 cells using anti-GRPEL1 antibody (AAA19632).Overlay histogram showing THP-1 cells stained with AAA19632 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRPEL1 Antibody (AAA19632, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GRPEL1, Polyclonal Antibody (Cat# AAA19632)
Full Name
Anti-GRPEL1 Antibody Picoband
Gene Names
GRPEL1; HMGE
Reactivity
Human
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
XRCC4, Polyclonal Antibody (Cat# AAA10755)
Full Name
XRCC4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using HLA-DPB1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
HLA-DPB1, Polyclonal Antibody (Cat# AAA22307)
Full Name
HLA-DPB1 Polyclonal Antibody
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody.)
UBR2, Polyclonal Antibody (Cat# AAA19207)
Full Name
Anti-UBR2 Antibody
Gene Names
UBR2; C6orf133; bA49A4.1; dJ242G1.1; dJ392M17.3
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(AAA31084 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
HSP90B, Polyclonal Antibody (Cat# AAA31084)
Full Name
HSP90B Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of Hela cells using anti-RLIM antibody (AAA19844).Overlay histogram showing Hela cells stained with AAA19844 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RLIM Antibody (AAA19844, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RLIM, Polyclonal Antibody (Cat# AAA19844)
Full Name
Anti-RLIM Antibody Picoband
Gene Names
RLIM; RNF12; NY-REN-43
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, FC/FACS, EIA
Purity
Immunogen affinity purified.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.