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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
We provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Viewing 9300-9350 of 14163 product results
ELISA
(Biotinylated Human IL-2 R alpha/CD25 Protein ELISA DataImmobilized Anti-CD25 Antibody, hFc Tag at 0.2?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA.)
IL-2 R alpha/CD25, Recombinant Protein (Cat# AAA76252)
Application Data
(Biotinylated Human EGFR/HER1 Protein SPR DataBiotinylated Human EGFR, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).)
EGFR/HER1, Recombinant Protein (Cat# AAA76154)
P26, Recombinant Protein (Cat# AAA78021)
SDS-PAGE
HPV 16, Recombinant Protein (Cat# AAA78023)
Application Data
(Figure-2: Comparative binding of Spike S1 variants to sACE2: Wells of a 96-well microtiter plate were coated with 100 ng in duplicates each of S1-WT, S1-N439K, S1-D614G, S1-South Africa, and S1-Southern California. Binding to sACE2 was determined by adding different concentrations of biotinylated-sACE2.)
SARS-CoV-2 Spike S1 Mutant Sampler Set, Recombinant Protein (Cat# AAA78598)
6-PGDH, Recombinant Protein (Cat# AAA78985)
COVID 19 Spike Protein E-Mosaic Coronavirus, Recombinant Protein (Cat# AAA78515)
Application Data
(Figure-2: Wells of a 96-microtiter plate were coated with 4ug/ml of SARS-Cov-2/nCov/COVID-19 Nucleocapsid recombinant protein. The binding was detected by addition of different dilution of polyclonal antibody. The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.)
COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA78527)
Application Data
(Figure 2: Binding assay of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein with recombinant Human ACE2 Fc Protein. An ELISA plate was coated with 1ug/ml of recombinant SARS CoV-2 Spike S1 C-Term His Tag Protein, and the plate was incubated overnight at 4 degree C. Different concentrations (100 ng/well to 0 ng/well) of recombinant Human ACE2 Fc Protein was added to the wells. HRP conjugated goat anti-human Fc was used as secondary antibody.)
COVID 19 Spike S1 Coronavirus, Recombinant Protein (Cat# AAA78555)
Application Data
(SDS-PAGE analysis of recombinant mouse soluble Podoplanin. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.)
Podoplanin, soluble, Recombinant Protein (Cat# AAA79189)
COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA226284)
ELISA
(ELISA for ADPRH/ARH1 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRH/ARH1 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
Application Data
(AMP-Glo assay for UBE2G2 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2G2 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2G2, Recombinant Protein (Cat# AAA60382)
Application Data
(AMP-Glo assay for UBE2E2 activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2E2 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2E2, Recombinant Protein (Cat# AAA60398)
Application Data
(AMP-Glo assay for UBE2Q2 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2Q2 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
UBE2Q2, Recombinant Protein (Cat# AAA60406)
Application Data
(ADP-Glo assay for SMARCA2/BRM activity 100 uM ATP and 10 nM Salmon sperm DNA was incubated with different concentrations of SMARCA2/BRM protein in a 10 ul reaction system containing 20 mM HEPES (pH 7.5) ,10mM MgCl2, 50 mM NaCl, 0.1%Tween-20, 1 mM DTT for 1 hour, then add 10 ul ADP-Glo Reagent at 25? for 60min.Then 20 ul Kinase. Detection Reagent incubates at 25? for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
HCV NS4, Recombinant Protein (Cat# AAA71894)
Zika Virus NS1 antigen, Recombinant Protein (Cat# AAA71913)
COVID 19 Spike S1 protein Coronavirus, Recombinant Protein (Cat# AAA71920)
Trypsinogen II, TG2 (human), Recombinant Protein (Cat# AAA59080)
C-Peptide, Recombinant Protein (Cat# AAA59091)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MEKK1, Recombinant Protein (Cat# AAA73654)
ZIP4, Recombinant Protein (Cat# AAA41030)
CLDN6, Recombinant Protein (Cat# AAA41037)
VEGFE, Recombinant Protein (Cat# AAA75460)
p15 Treponema Pallidum, Recombinant Protein (Cat# AAA75467)
Bacillus Anthracis, Recombinant Protein (Cat# AAA75287)
Cyclin-Dependent Kinase Inhibitor 2A, Recombinant Protein (Cat# AAA38569)
Tobacco Etch Virus Protease, Recombinant Protein (Cat# AAA38573)
RAB5A, Recombinant Protein (Cat# AAA38576)
Activin-A, Recombinant Protein (Cat# AAA38584)
Pigment Epithelium-Derived Factor, Recombinant Protein (Cat# AAA38598)
TNF-Related Apoptosis Inducing Ligand/Apo2L, Recombinant Protein (Cat# AAA38606)
Myostatin, Recombinant Protein (Cat# AAA38209)
Resistin, Recombinant Protein (Cat# AAA38216)
Formiminotransferase Cyclodeaminase, Recombinant Protein (Cat# AAA38266)
Inhibin-Alpha A Chain, Recombinant Protein (Cat# AAA38367)
Hemagglutinin-Influenza A Virus H3N2 Wisconsin 67/05, Recombinant Protein (Cat# AAA38380)
Kinase Akt1/PKB alpha Active Enzyme, Recombinant Protein (Cat# AAA38386)
Thrombospondin, Recombinant Protein (Cat# AAA38420)
Tripartite Motif Containing 21, Recombinant Protein (Cat# AAA38432)
Single Chain Cardiac Troponin I-C, Recombinant Protein (Cat# AAA38444)
Ubiquitin Biotin, Recombinant Protein (Cat# AAA38640)
Aurora Kinase B, Recombinant Protein (Cat# AAA38654)
Ferredoxin-1, Recombinant Protein (Cat# AAA38656)
Tumor Necrosis Factor Receptor, Recombinant Protein (Cat# AAA38658)
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
SMAD Family Member 2, Recombinant Protein (Cat# AAA38663)
SNAP, Recombinant Protein (Cat# AAA38664)
Dopa Decarboxylase, Recombinant Protein (Cat# AAA38670)
Interleukin-1 Receptor Like-1, Recombinant Protein (Cat# AAA38679)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression: The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification: After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use. Learn more in our guide on “factors affecting recombinant protein yield”.
The result is a purified recombinant protein, a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests, often supported by high-quality ELISA kits for accurate quantification.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency, two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.