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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 1250-1300 of 14163 product results
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CK1 gamma 2, Recombinant Protein (Cat# AAA73378)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PDGFR alpha, Recombinant Protein (Cat# AAA73794)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PDGFR alpha (D842V), Recombinant Protein (Cat# AAA73795)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TPM3-PDGFR beta, Recombinant Protein (Cat# AAA73801)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPN2 (TC-PTP), Recombinant Protein (Cat# AAA73810)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
GRK6, Recombinant Protein (Cat# AAA73578)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EPHA1, Recombinant Protein (Cat# AAA73512)
SDS-PAGE
(The purity of 1kBalpha was determined to be >90% by densitometry.)
IkBA, Recombinant Protein (Cat# AAA72909)
SDS-PAGE
(The purity of KDM1A was determined to be >95% by densitometry. Approx. MW 100kDa.)
KDM1A, Recombinant Protein (Cat# AAA72910)
Application Data
KAT7, Recombinant Protein (Cat# AAA73000)
Application Data
(The specific activity of TPM3-TRKA was determined to be 13 nmol /min/mg as per activity assay protocol.)
TPM3-TRKA, Recombinant Protein (Cat# AAA73035)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
BIRC7, Recombinant Protein (Cat# AAA73283)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CK1 alpha 1, Recombinant Protein (Cat# AAA73374)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(The purity of RFP was determined to be >90% by densitometry. Approx. MW 29 kDa)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPN12 (PTP-PEST), Recombinant Protein (Cat# AAA73830)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(The purity of IRAK1 was determined to be >90% by densitometry. Approx. MW 132 kDa.)
IRAK1, Recombinant Protein (Cat# AAA73617)
Application Data
(Recombinant DNMT3A protein activity assay Recombinant DNMT3A protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
DNMT3A, Recombinant Protein (Cat# AAA59662)
Application Data
(HTRF assay for TBP activity 1 uM oligo dsDNA (DNA sequence: 5’-TCGTATAAAAGGC-3’) was incubated with different concentrations of TBP protein in 10 ul system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
TBP, Recombinant Protein (Cat# AAA60140)
Application Data
(HTRF assay for BRD3 (24-144), GST-Tag activity 3 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD3 (24-144), GST-Tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
BRD3, Recombinant Protein (Cat# AAA60160)
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant polynucleosomes and histone octamers as substrates. 2 ug Recombinant Polynucleosomes H3.3 (K36M) were incubated with DOT1L in reaction buffer for 3 hours at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for DOT1L than histone octamers.)
Polynucleosomes H3.3, Recombinant Protein (Cat# AAA59752)
Application Data
(HTRF Assay for Recombinant TRIM24 (862-980) activity. 3 uM histone peptide H3K14ac was incubated with TRIM24 (862-980) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. )
TRIM24, Recombinant Protein (Cat# AAA59640)
Application Data
(HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
PDK1, Recombinant Protein (Cat# AAA60178)
Application Data
(Recombinant SETD7 protein activity assay. 2 ug Calf Thymus Octamers was incubated with 0.5 ug SETD7 protein in reaction buffer containing radioactive 3H-SAM for 2 hour at room temperature. Activity was detected by fluorography.)
SETD7, Recombinant Protein (Cat# AAA59717)
Application Data
(HTRF assay for JAK3 (781-1124) activity 1 uM TK substrate was incubated with different concentrations of JAK3 (781-1124) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JAK3, Recombinant Protein (Cat# AAA60244)
Application Data
(HTRF assay for STK11 / LKB1 activity 1 uM STK S1 substrate was incubated with different concentrations of STK11 / LKB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
STK11/LKB1, Recombinant Protein (Cat# AAA60249)
Application Data
KAT2B, Recombinant Protein (Cat# AAA72995)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SAE1/UBA2 Complex, Recombinant Protein (Cat# AAA74080)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
IKK beta, Recombinant Protein (Cat# AAA73613)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ERN1 (IRE1), Recombinant Protein (Cat# AAA73528)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ERN1 (R727A), Recombinant Protein (Cat# AAA73529)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ERK7 (MAPK15), Recombinant Protein (Cat# AAA73682)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CK1 gamma 3, Recombinant Protein (Cat# AAA73379)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TRKC (L686M), Recombinant Protein (Cat# AAA73770)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
AMPK (A2/B2/G1), Recombinant Protein (Cat# AAA73842)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RET (R749T), Recombinant Protein (Cat# AAA73896)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RET (S904A), Recombinant Protein (Cat# AAA73909)
WB (Western Blot)
(Western Blot analysis for Recombinant Histone H3K79me3 (MLA) Recombinant Histone H3K27me3 (MLA) (Lane 1) and Histone H3.1 (Lane 2) were detected with H3K79me2me3 antibody . Histones stained by coomassie blue below were as loading control.)
Histone H3K79me3, Recombinant Protein (Cat# AAA59760)
WB (Western Blot)
(Recombinant Mononucleosomes H3K27ac - biotin tested by Western blot Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes (H3K27ac) - biotinylated (Lane 2) were detected with anti-H3K27ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes (H3K27ac) - biotinylated can be detected by anti-H3K27ac antibody.)
Mononucleosomes H3K27ac, Recombinant Protein (Cat# AAA60099)
Application Data
MBP, Recombinant Protein (Cat# AAA72939)
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant nucleosomes and histone octamers as substrates. 2 ug Recombinant mononucleosomes (H3.1) were incubated with 0 ug (-), 0.05 ug (+), 0.1 ug (++), 0.2 ug (+++) NSD2-SET protein, respectively, in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H3K36me2). The Western blot result shows that recombinant mononucleosomes (H3.1) are better substrate than histone octamer for NSD2-SET assay.)
Mononucleosomes, Recombinant Protein (Cat# AAA59701)
Application Data
JAK1, Recombinant Protein (Cat# AAA72993)
Application Data
RAC2, Recombinant Protein (Cat# AAA73115)
Application Data
(Recombinant PTPN1 (1-321) activity assay. 1 mM pNPP was incubated with different concentrations of PTPN1 protein in 100 ul reaction system including 50 mM HEPES pH 7.5, 2 mM EDTA, 3 mM DTT, 100 mM NaCl at room temperature. The absorbance at 405 nm was continuously monitored for 30 minutes.)
PTPN1, Recombinant Protein (Cat# AAA60114)
SDS-PAGE
(Figure A:Product purity determined by Coomassie staining following SDS-PAGEFigure B: Western Blot of thioester assays (TE +ve/-VE controls) for E2 conjugating enzymes. Biotinylated-ubiquitin-enzyme conjugates were detected by western blotting on thioester assays containing: A: UbcH2, B: UbcH8, respectively, using Streptavidin-HRP detection system. M: Biotinylated SDS molecular weight markers (Sigma, SDS-6B) from bottom: 20.1, 29.0, 39.8, 58.1 kDa)
Ubiquitin Activating Enzyme E1, Recombinant Protein (Cat# AAA77053)
Purified by multi-step chromatography
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ANXA4-PKN1, Recombinant Protein (Cat# AAA73870)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.