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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 1100-1150 of 14163 product results
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKC delta, Recombinant Protein (Cat# AAA73861)
Application Data
(SARS-CoV-2 Spike protein, S1 RBD Competition and Blocking Activity Assay A) SARS-CoV-2 Spike Antibody (clone AM001414) competes with ACE2 receptor for RBD binding. SARS-CoV-2 Spike Antibody (clone AM001414) was serially diluted in the presence of 100 ng/mL biotinylated ACE2 receptor, then added to wells coated with 1 ug/mL RBD, aa 319-589 for 30 min. Streptavidin-HRP was then added to detect bound ACE2 receptor. B) SARS-CoV-2 Spike Antibody (clone AM001414) blocks ACE2 receptor from binding to RBD. Serial dilutions of SARS-CoV-2 Spike Antibody (clone AM001414) was added to wells coated with 1 ug/mL RBD, aa 319-589. 100 ng/mL biotinylated ACE2 receptor was then added for 30 min, followed by Streptavidin-HRP to detect bound ACE2 receptor.)
COVID 19 Spike, S1 RBD Coronavirus, Recombinant Protein (Cat# AAA60225)
Application Data
(HTRF assay for recombinant AKT1 protein activity 1 uM STK S3 substrate was incubated with different concentrations AKT1 protein a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE STK assay was used to detect the enzymatic activity.)
AKT1, Recombinant Protein (Cat# AAA60154)
WB (Western Blot)
(Recombinant Mononucleosomes (H3.3) - biotinylated activity. 2 ?g Mononucleosomes were incubated with 0 ?g, 0.25 ?g, 0.5 ?g, 1 ?g NSD2-SET in reaction buffer for 3 h at room temperature, respectively. Western blot was used for detecting the generation of reaction products (H3K36me2). Recombinant Histone Octamers was used as a control substrate. The Western blot result shows that mononucleosomes are a more suitable substrate for NSD2-SET than histone octamers.)
Mononucleosomes, Recombinant Protein (Cat# AAA59703)
Application Data
RAC1, Recombinant Protein (Cat# AAA73114)
Application Data
GSK3beta, Recombinant Protein (Cat# AAA72988)
Application Data
JAK1, Recombinant Protein (Cat# AAA72992)
Application Data
(Recombinant IDH2 (R140K) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH2 (R140K) protein in 200 ul reaction system (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA, room temp). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)
IDH2, Recombinant Protein (Cat# AAA59786)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TRKA (L657M), Recombinant Protein (Cat# AAA73760)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPN11 (SHP2), Recombinant Protein (Cat# AAA73828)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CAMK1, Recombinant Protein (Cat# AAA73301)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
FGFR3, Recombinant Protein (Cat# AAA73550)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
c-KIT (V559D V654A), Recombinant Protein (Cat# AAA73634)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MEK3 (MAP2K3), Recombinant Protein (Cat# AAA73650)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ALK (L1196M G1202R), Recombinant Protein (Cat# AAA73235)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPN11 (SHP2), Recombinant Protein (Cat# AAA73827)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPN13 (FAP-1), Recombinant Protein (Cat# AAA73831)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKN1/PRK1, Recombinant Protein (Cat# AAA73868)
WB (Western Blot)
(Western Blot analysis for Recombinant Mononucleosomes H3K4me1 (EPL) - biotinylated. Unmodified mononucleosomes (Lane 1) and Mononucleosomes H3K4me1 (Lane 2) were detected with anti-H3K4me1 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Mononucleosomes H3K4me1 can be detected by anti- H3K4me1 antibody. Nucleosomes = Mononucleosomes)
Mononucleosomes H3K4me1, Recombinant Protein (Cat# AAA59764)
Application Data
(Recombinant IDH2 (R140Q) protein activity assay 20 uM NADPH and 1 uM a-Ketoglutaric acid were incubated with 500 nM IDH2 (R140Q) protein in a reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA at room temperature. Depletion of NADPH was monitored continuously at absorbance 340 nm for 60 min.)
IDH2, Recombinant Protein (Cat# AAA59787)
SDS-PAGE
(Recombinant Mononucleosomes H3.3 (R8C) 12.5% SDS-PAGE gel with Coomassie Blue staining MW: 108 kDa Purity: >95%)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60196)
Application Data
(Recombinant p300 (1041-1161) activity using AlphaScreen. p300 (1041-1161) titration and inhibition were assessed using an AlphaScreen assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner.)
p300, Recombinant Protein (Cat# AAA59642)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (R8C) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60207)
Application Data
AKT3, Recombinant Protein (Cat# AAA72943)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TIE2 (R849W), Recombinant Protein (Cat# AAA73987)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
DAPK1, Recombinant Protein (Cat# AAA73389)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RET (M918T), Recombinant Protein (Cat# AAA73901)
Application Data
(HTRF assay for JAK1 (438-1154) activity 1 uM TK substrate was incubated with different concentrations of JAK1 (438-1154) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JAK1, Recombinant Protein (Cat# AAA60240)
Application Data
(HTRF assay for RXR-a (RXRA) activity 1 ?M dsDNA oligos (DNA sequence: 5’-ATAGGTCACGGAGAGGTCAGC-3’) were incubated with different concentrations of protein in 10 ?l binding system containing 50 mM HEPESNaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ?l anti-DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
RXR-alpha (RXRA), Recombinant Protein (Cat# AAA60126)
SDS-PAGE
STUB1 (CHIP), Recombinant Protein (Cat# AAA72894)
Application Data
p70S6K, Recombinant Protein (Cat# AAA73121)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
KLC1-ALK, Recombinant Protein (Cat# AAA73239)
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant polynucleosomes and histone octamers as substrates. 2 ug Recombinant Polynucleosomes H3.3 (G34R) were incubated with DOT1L in reaction buffer for 3 hours at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for DOT1L than histone octamers.)
Polynucleosomes H3.3, Recombinant Protein (Cat# AAA59749)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EIF2AK2, Recombinant Protein (Cat# AAA73882)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
HDAC8, Recombinant Protein (Cat# AAA73605)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
c-KIT (D816I), Recombinant Protein (Cat# AAA73638)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CK1 delta, Recombinant Protein (Cat# AAA73375)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
UBE2C, Recombinant Protein (Cat# AAA74082)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Functional Data
(Binding curve of anti-Cov-19&SARS S glycoprotein antibody IgG CR3022 to the trimeric Spike B.1.617.2, Delta variant.)
COVID 19 Spike protein, Delta B.1.617.2 variant Coronavirus, Recombinant Protein (Cat# AAA47146)
Application Data
(HTRF assay for HDAC8 protein activity.3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
HDAC8, Recombinant Protein (Cat# AAA59741)
SDS-PAGE
4EBP1, Recombinant Protein (Cat# AAA72932)
SDS-PAGE
(Purified recombinant spike RBD (K417T, E484K, N501Y) protein of SARS-CoV-2 Brazil variant)
COVID 19 Spike RBD Brazil Variant Coronavirus, Recombinant Protein (Cat# AAA62224)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
MSR1, Recombinant Protein (Cat# AAA48896)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.