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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 1300-1350 of 14163 product results
SDS-PAGE
(The purity of PKD2 (G848E) was determined to be >90% by densitometry, approx. MW 130 kDa.)
PKD2 (PRKD2)(G848E), Recombinant Protein (Cat# AAA73877)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKD2 (PRKD2)(G870E), Recombinant Protein (Cat# AAA73878)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PRKG2, Recombinant Protein (Cat# AAA73881)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
RET (V778I), Recombinant Protein (Cat# AAA73903)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
DDR1 (S496A), Recombinant Protein (Cat# AAA73393)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TRKC (G696A), Recombinant Protein (Cat# AAA73772)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TRKC (G696C), Recombinant Protein (Cat# AAA73773)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(The purity of PTPRM was determined to be >90% by densitometry. Approx. MW 110kDa)
PTPRM (PTP- mu), Recombinant Protein (Cat# AAA72922)
Application Data
RhoA, Recombinant Protein (Cat# AAA73120)
Application Data
MEK1, Recombinant Protein (Cat# AAA73012)
Application Data
JNK1, Recombinant Protein (Cat# AAA73019)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
BIRC3, Recombinant Protein (Cat# AAA73282)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ROCK2, Recombinant Protein (Cat# AAA73924)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ZAP70 (Y319F), Recombinant Protein (Cat# AAA74126)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TRKC (G623R L686M), Recombinant Protein (Cat# AAA73771)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EPHB2, Recombinant Protein (Cat# AAA73520)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
HDAC8, Recombinant Protein (Cat# AAA73606)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
HDAC10, Recombinant Protein (Cat# AAA73608)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPRS (PTP-sigma), Recombinant Protein (Cat# AAA73862)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MYLK3, Recombinant Protein (Cat# AAA73732)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Application Data
(HTRF for IGF2BP3 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP3 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
IGF2BP3, Recombinant Protein (Cat# AAA60211)
Application Data
(HTRF assay for PIM2 activity 1 uM STK S3 substrate was incubated with different concentrations of PIM2 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JARID1C / KDM5C activity. 1 uM H3K4me3 peptide was incubated with JARID1C / KDM5C protein in reaction buffer for 1 hour at room temperature.H3K4me2 antibody was used to detect reaction product. )
JARID1C/KDM5C, Recombinant Protein (Cat# AAA59681)
WB (Western Blot)
(Western Blot analysis of Recombinant Mononucleosomes H3K4me3/H3K27ac - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K4me3/K27ac - biotiny (Lane 2) were detected with antibody anti-H3K4me3, anti-H3K27ac and anti-H4, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K4me3/K27ac - biotin can be detected by anti-H3K4me3 and anti- H3K27ac antibody.)
Mononucleosomes H3K4me3/H3K27ac, Recombinant Protein (Cat# AAA60100)
TB CFP10, Recombinant Protein (Cat# AAA27917)
Application Data
(Recombinant Mononucleosomes (H2A.Z) DNA gel Mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes)
Mononucleosomes, Recombinant Protein (Cat# AAA60120)
WB (Western Blot)
(Recombinant p300 protein activity assay 0.5 ug of Histone H4 were incubated with 0 (-), 12.5 (+), 25 (++), or 50 (+++) ng of p300 protein, respectively in a 20 ul reaction system including 50 mM Tris pH 8.0, 1 mM TCEP, 0.1 mM EDTA, 50 ng/ul BSA and 20 uM acetyl-CoA for 30 minutes at 30 degree C. For each reaction, 7.5 ul product was run on a 12.5% SDS-PAGE gel and detected by Western Blot. p300 only was used as negative control. H4 pan-acetyl antibody was used to recognize products.)
p300, Recombinant Protein (Cat# AAA60167)
Application Data
(HTRF Assay for Recombinant SMARCA4/BRG1 (1448-1569) Activity. 3.3 uM histone peptide H3K14ac was incubated with SMARCA4/BRG1 (1448-1569) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
SMARCA4/BRG1, Recombinant Protein (Cat# AAA59660)
Application Data
(Recombinant DNMT3B protein activity assay Recombinant DNMT3B protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
DNMT3B, Recombinant Protein (Cat# AAA59665)
Application Data
(Recombinant TRIM28 (624-811) activity assay. 3.3 uM histone peptide H4K5/8/12/16 (4ac) was incubated with TRIM28 (624-811) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
TRIM28, Recombinant Protein (Cat# AAA59685)
Application Data
IKKbeta, Recombinant Protein (Cat# AAA72989)
Application Data
JNK3, Recombinant Protein (Cat# AAA73025)
SDS-PAGE
(Purity: Sample purity data. For lot-specific data please refer to the specific datasheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EPHB1, Recombinant Protein (Cat# AAA73519)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ERN1 (IRE1), Recombinant Protein (Cat# AAA73532)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PFKFB3, Recombinant Protein (Cat# AAA73818)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EGFR (d746-750 T790M C797S), Recombinant Protein (Cat# AAA73468)
DB (Dot Blot)
(Dot blot for Recombinant FTO protein activity 1 ?M m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with different concentrations of FTO in reaction buffer including 25 mM Tris-HCl pH 7.5, 100 ?M 2-oxoglutarate, 100 ?M ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hr at room temperature. The 20 ?l reactions were concentrated to 5 ?l. 1 ?l of products were spotted onto positively charged nylon membrane, then detected with m6A antibody . Dot blot was used for activity detection.)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.