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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
We provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Viewing 1150-1200 of 15249 product results
Application Data
(The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 uM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 uM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent AAA283374, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data))
Application Data
(Immobilized SARS-CoV-2 Envelope at 2 ug/mL (100 uL/well) can bind SARS-COV-2 Nucleocapsid with a linear range of 0.01-30 ng/mL.)
SARS-COV-2 Nucleocapsid (D63G, R203M, D377Y), Recombinant Protein (Cat# AAA282535)
Application Data
(Recombinant Mouse IL-13 stimulates cell proliferation of the TF-1 Mouse erythroleukemic cells. The ED50 for this effect is 23.9-95.6 ng/mL.)
IL-13, Recombinant Protein (Cat# AAA282570)
Bioactivity
(ActivityMeasured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 ug/ml can bind Anti-CLDN18.2 recombinant Antibody , the EC50 is 2.806-3.641 ng/ml.)
Claudin (CLDN18), Recombinant Protein (Cat# AAA279275)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
SPI-1 type 3 secretion system translocon protein SctE (sctE1), Recombinant Protein (Cat# AAA279401)
Probable E3 ubiquitin-protein ligase makorin-3 (Mkrn3), Recombinant Protein (Cat# AAA233159)
Application Data
(Loaded Anti-COVID-19 S1 mAb on Protein A Biosensor, can bind Recombinant COVID-19 S-trimer Protein (Omicron, B.1.1.529) with an affinity constant of 10.7 nM as determined in bioactivity assay.)
COVID-19 S-trimer, Recombinant Protein (Cat# AAA266228)
Dihydropyrimidinase-related protein 2 (Dpysl2), Recombinant Protein (Cat# AAA113797)
Centromere protein F (CENPF), Recombinant Protein (Cat# AAA113372)
Gastric triacylglycerol lipase (LIPF), Recombinant Protein (Cat# AAA113299)
Replication protein A 14 kDa subunit (RPA3), Recombinant Protein (Cat# AAA113534)
Cyclin-dependent kinase 5 activator 1 (Cdk5r1), Recombinant Protein (Cat# AAA113716)
Signal transducer and activator of transcription 3 (Stat3), Recombinant Protein (Cat# AAA113730)
Single Ig IL1 Related Receptor (SIGIRR), Recombinant Protein (Cat# AAA134329)
Ubiquitin-conjugating enzyme E2 10 (UBC10), Recombinant Protein (Cat# AAA114574)
Phospholipase A1 (pldA), Recombinant Protein (Cat# AAA114746)
Protein Wiz (WIZ), Recombinant Protein (Cat# AAA114776)
HLA class II histocompatibility antigen, DRB1-3 chain (HLA-DRB1), Recombinant Protein (Cat# AAA115054)
Acid ceramidase (ASAH1), Recombinant Protein (Cat# AAA114665)
Matrix protein 1 (M), Recombinant Protein (Cat# AAA114679)
ESX-1 secretion-associated protein EspA (espA), Recombinant Protein (Cat# AAA114495)
Small t antigen, Recombinant Protein (Cat# AAA114540)
Exotoxin A (eta), Recombinant Protein (Cat# AAA114939)
Dihydrolipoyl dehydrogenase, Recombinant Protein (Cat# AAA114867)
Insulin-2 (Ins2), Recombinant Protein (Cat# AAA114889)
Peptidoglycan recognition protein 1 (Pglyrp1), Recombinant Protein (Cat# AAA114901)
Leukotoxin (lktA), Recombinant Protein (Cat# AAA116036)
Vitamin D-binding protein (Gc), Recombinant Protein (Cat# AAA115871)
Glutamyl endopeptidase (sspA), Recombinant Protein (Cat# AAA115947)
Myocardin (MYOCD), Recombinant Protein (Cat# AAA116615)
Staphylococcus epidermidis mRNA interferase MazF (mazF), Recombinant Protein (Cat# AAA116697)
Melanoma-associated antigen C2 (MAGEC2), Recombinant Protein (Cat# AAA116700)
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform (PPP2R2C), Recombinant Protein (Cat# AAA116556)
Striatin-3 (STRN3), Recombinant Protein (Cat# AAA116913)
Immediate-early protein 1 (U90/U89), Recombinant Protein (Cat# AAA117180)
60 kDa chaperonin (groL), Recombinant Protein (Cat# AAA115366)
Interferon gamma (IFNG), Recombinant Protein (Cat# AAA115687)
Type III export protein pscF (pscF), Recombinant Protein (Cat# AAA115244)
Guanine nucleotide-binding protein subunit beta-like protein, Recombinant Protein (Cat# AAA115171)
MADCAM1 / Mucosal Addressin Cell Adhesion Molecule 1, Recombinant Protein (Cat# AAA196941)
Cardiotrophin Like Cytokine Factor 1 (CLCF1), Recombinant Protein (Cat# AAA149293)
MYL3, Recombinant Protein (Cat# AAA119937)
CD44/MIC4, Recombinant Protein (Cat# AAA174009)
Human C-Reactive Protein, Recombinant Protein (Cat# AAA78968)
a single band (1 ug/lane)
Recombinant human papillomavirus 6 large capsid, Recombinant Protein (Cat# AAA78025)
Mimecan, Recombinant Protein (Cat# AAA309736)
rDau c 1, Recombinant Protein (Cat# AAA129083)
SDS-PAGE
(Recombinant Human Hedgehog- interacting protein/HHIP Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.)
Hip, Recombinant Protein (Cat# AAA281513)
SDS-PAGE
(Recombinant Human CD204 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 55-75 kDa.)
CD204, Recombinant Protein (Cat# AAA281520)
Application Data
(Recombinant Human Flt3 ligand/Flt3L stimulates cell proliferation assay using Human OCI-AML5 cells. The ED<sub>50</sub> for this effect is 1.56-6.24 ng/mL, corresponding to a specific activity of 1.60×10<sup>5</sup>~6.41×10<sup>5</sup> units/mg.)
Flt3 ligand/Flt3L, Recombinant Protein (Cat# AAA283382)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression: The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification: After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use. Learn more in our guide on “factors affecting recombinant protein yield”.
The result is a purified recombinant protein, a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests, often supported by high-quality ELISA kits for accurate quantification.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency, two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.