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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Recombinant Proteins" - showing 8350-8400
Application Data
(HTRF assay for GSK3b activity 1 uM STK S3 substrate was incubated with different concentrations of GSK3b protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffinembedded human uterus using Tropomyosin 1 antibody (AAA37156) at dilution of 1:100 (40x lens).)
TPM1, Polyclonal Antibody (Cat# AAA37156)
Full Name
TPM1 Polyclonal Antibody
Gene Names
TPM1; CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HTM-alpha
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human VAMP1 protein Lane 2.: Recombinant human VAMP2 protein Lane 3.: Recombinant human VAMP3 protein)
VAMP-2, Monoclonal Antibody (Cat# AAA48351)
Full Name
VAMP-2
Gene Names
VAMP2; SYB2; VAMP-2; NEDHAHM
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot)
(Anti-EPHB3 rabbit polyclonal antibody at 1:500 dilution.Lane A: Hela Whole Cell LysateLane B: Mouse heart tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:110 kDaObserved band size:110 kDa(We are unsure as to the identity of these extra bands.))
EphB3, Polyclonal Antibody (Cat# AAA257346)
Full Name
Anti-EphB3 Antibody, Rabbit Polyclonal
Reactivity
Rat
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
Bioactivity
(Detects Lexatumumab in indirect ELISAs.)
CD262/TNFRSF10B/TRAIL-R2, Recombinant Protein (Cat# AAA120536)
Full Name
Recombinant Human CD262/TNFRSF10B/TRAIL-R2, C-Fc
Gene Names
TNFRSF10B; DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5
Applications
Western Blot
Purity
>90% as determined by SDS-PAGE
Pricing
WB (Western Blot)
(Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid rabbit monoclonal antibody at 1:5000 dilution.Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.)
SARS-CoV-2 (2019-nCoV) Nucleocapsid, Monoclonal Antibody (Cat# AAA257785)
Full Name
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb
Reactivity
SARS-CoV-2
Applications
Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Protein A
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of CKMT1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKMT1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
CKMT1A, Monoclonal Antibody (Cat# AAA48140)
Full Name
CKMT1A antibody
Gene Names
CKMT1A; CKMT1; U-MtCK; mia-CK
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Anti-MyD88 Picoband antibody, AAA46031-5.JPGIHC(P): Mouse Spleen Tissue)
Myeloid differentiation primary response protein MyD88, Polyclonal Antibody (Cat# AAA46031)
Full Name
Anti-MyD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant PEX5 protein using PEX5 antibody)
PEX5, Monoclonal Antibody (Cat# AAA107372)
Full Name
PEX5 antibody
Gene Names
PEX5; PXR1; PBD2A; PBD2B; PTS1R; PTS1-BP
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
PEX5 antibody was purified by affinity chromatography.
Pricing
WB (Western Blot)
(Anti-TNFRI rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: 293T Whole Cell LysateLane C: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.))
TNFRSF1A, Polyclonal Antibody (Cat# AAA255231)
Full Name
Anti-TNFRSF1A Antibody, Rabbit Polyclonal
Gene Names
TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Anti-STUB1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: MCF7 Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))
STUB1, Polyclonal Antibody (Cat# AAA255589)
Full Name
Anti-STUB1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
Application Data
(Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
MLLT3/AF9, Recombinant Protein (Cat# AAA60171)
Full Name
Recombinant MLLT3/AF9 (1-138) protein, His/DYKDDDDK Tag
Gene Names
MLLT3; AF9; YEATS3
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human nerve and ganglion cells using S100A10/P11 antibody.)
p11, Monoclonal Antibody (Cat# AAA106381)
Full Name
p11 antibody
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human esophagus using PHB2 antibody at dilution of 1:100 (40x lens).)
PHB2, Polyclonal Antibody (Cat# AAA37525)
Full Name
PHB2 Polyclonal Antibody
Gene Names
PHB2; BAP; REA; p22; Bap37; BCAP37; PNAS-141
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Anti-PPM1A rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:40 kDa(We are unsure as to the identity of these extra bands.))
PPM1A, Polyclonal Antibody (Cat# AAA257123)
Full Name
Anti-PPM1A Antibody, Rabbit Polyclonal
Gene Names
Ppm1a; MMPa-2; MPPa-1; AI427932; AU017636; 2310003C21Rik; 2900017D14Rik
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
Application Data
(HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 ?M ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Amino Acid Info
Eukaryotic Interferon Gamma (IFNg), Protein (Cat# AAA153148)
Full Name
Eukaryotic Interferon Gamma (IFNg)
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NUBPL protein (Right) or empty vector (Left) detected with NUBPL antibody)
NUBPL, Monoclonal Antibody (Cat# AAA107431)
Full Name
NUBPL antibody
Gene Names
NUBPL; IND1; huInd1; C14orf127
Applications
Western Blot, Flow Cytometry
Purity
NUBPL antibody was purified by affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(MAP3K8 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- MAP3K8 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
MAP3K8, Polyclonal Antibody (Cat# AAA46649)
Full Name
Anti-MAP3K8 Antibody
Gene Names
MAP3K8; COT; EST; ESTF; TPL2; AURA2; MEKK8; Tpl-2; c-COT
Reactivity
Human, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(ICC/IF analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
AK1, Monoclonal Antibody (Cat# AAA48262)
Full Name
AK1 antibody
Gene Names
AK1; HTL-S-58j
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot
Purity
By protein-A affinity chromatography
Pricing
WB (Western Blot)
(Anti-ISG15 rabbit monoclonal antibody at 1:5000 dilutionLane A: Hela Whole Cell LysateLane B: 293 Whole Cell LysateLane C: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:15 kDa)
ISG15, Monoclonal Antibody (Cat# AAA255607)
Full Name
Recombinant Anti-ISG15 Antibody, Rabbit Monoclonal
Gene Names
ISG15; G1P2; IP17; UCRP; IFI15; hUCRP
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A
Pricing
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human YWHAB expression on Molt-4 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
14-3-3 beta, Monoclonal Antibody (Cat# AAA255220)
Full Name
Anti-14-3-3 beta Antibody, Mouse Monoclonal
Gene Names
YWHAB; HS1; GW128; YWHAA; KCIP-1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
Protein A
Pricing
WB (Western Blot)
(Anti-METAP2 rabbit polyclonal antibody at 1:500 dilutionLane A: METAP2 konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:72 kDa)
methionyl aminopeptidase 2/METAP2, Polyclonal Antibody (Cat# AAA254758)
Full Name
Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Polyclonal
Gene Names
METAP2; MAP2; MNPEP; p67eIF2
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
WB (Western Blot)
(Western blot All lanes: 60S ribosomal protein L11 antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 118kDa Additional bands at: 25,30,40?0 kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L11, Polyclonal Antibody (Cat# AAA81404)
Full Name
Rabbit anti-human 60S ribosomal protein L11 polyclonal Antibody, HRP conjugated
Gene Names
RPL11; L11; DBA7; GIG34
Reactivity
Human
Applications
ELISA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using SUMO4. Blue: DAPI for nuclear staining.)
SUMO4, Polyclonal Antibody (Cat# AAA314057)
Full Name
SUMO4 Rabbit Polyclonal Antibody
Gene Names
SUMO4; IDDM5; SMT3H4; SUMO-4; dJ281H8.4
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity purification
Pricing
Amino Acid Info
Eukaryotic Interleukin 3 Receptor Alpha (IL3Ra), Protein (Cat# AAA153212)
Full Name
Eukaryotic Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Applications
Western Blot
Purity
Greater than 90% by SDS-PAGE
Pricing
IHC (Immunohistochemisry)
(DAB staining on IHCP;Samples: Rat Lung Tissue;Primary Ab: 30ug/ml Rabbit AntiRat PCX AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiRabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19))
Podocalyxin (PODXL), Polyclonal Antibody (Cat# AAA151937)
Full Name
Polyclonal Antibody to Podocalyxin (PODXL)
Gene Names
Podxl; PC; PCLP-1; podocalyxin
Reactivity
Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigenspecific affinity chromatography followed by Protein A affinity chromatography
Pricing
Application Data
(Recombinant KMT2A (MLL1) complex activity assay. 3.3 ?M H3K4me0 (1-21aa) peptide was incubated with MLL1 complex in reaction buffer for 3 hour at room temperature. MLL1 complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody.)
KMT2A, Recombinant Protein (Cat# AAA59674)
Full Name
Recombinant KMT2A (MLL1) complex
Gene Names
KMT2A; HRX; MLL; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL/GAS7; TET1-MLL
Purity
The recombinant protein is >95% pure by SDS-PAGE.
Pricing
WB (Western Blot)
(Figure 4. Western blot analysis of PSD95 using anti- PSD95 antibody (AAA46432).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PSD95 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PSD95 at approximately 95KD. The expected band size for PSD95 is at 80KD.)
PSD95, Polyclonal Antibody (Cat# AAA46432)
Full Name
Anti-PSD95 Antibody
Gene Names
DLG4; PSD95; SAP90; SAP-90
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of Cardiac FABP using anti-Cardiac FABP antibody.Cardiac FABP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cardiac FABP antibody.Cardiac FABP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-Cardiac FABP antibody.Cardiac FABP was detected in paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Cardiac FABP antibody.Cardiac FABP was detected in paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Cardiac FABP Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cardiac FABP/FABP3, Polyclonal Antibody (Cat# AAA124933)
Full Name
Anti-Cardiac FABP/FABP3 Antibody
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using MMP3 Mouse Monoclonal Antibody (MMP3/2655). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
MMP3, Monoclonal Antibody (Cat# AAA214780)
Full Name
MMP3 (Marker of Metastasis and Rheumatoid Arthritis)
Gene Names
MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1
Reactivity
Human. Others not tested.
Applications
Western Blot
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Hela cells using anti-RAB14 antibody (AAA127221).Overlay histogram showing Hela cells stained with AAA127221 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB14 Antibody (AAA127221, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RAB14, Polyclonal Antibody (Cat# AAA127221)
Full Name
Anti-RAB14 Antibody Picoband
Gene Names
RAB14; FBP; RAB-14
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-PLA2G3 antibody (AAA127483).Overlay histogram showing HepG2 cells stained with AAA127483 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLA2G3 Antibody (AAA127483, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PLA2G3, Polyclonal Antibody (Cat# AAA127483)
Full Name
Anti-PLA2G3 Antibody Picoband
Gene Names
PLA2G3; SPLA2III; sPLA2-III; GIII-SPLA2
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of Caspase 4 using anti-Caspase 4 antibody (AAA124698).Caspase 4 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Caspase 4 Antibody (AAA124698) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Caspase 4, Polyclonal Antibody (Cat# AAA124698)
Full Name
Anti-Caspase 4 Picoband antibody
Gene Names
CASP4; TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using C1QB Mouse Monoclonal Antibody (C1QB/2965).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
C1QB/Complement C1q B-Chain, Monoclonal Antibody (Cat# AAA215226)
Full Name
C1QB/Complement C1q B-Chain
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Filaggrin Mouse Monoclonal Antibody (FLG/1563).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Filaggrin, Monoclonal Antibody (Cat# AAA214646)
Full Name
Filaggrin (Keratinocyte Differentiation Marker)
Gene Names
FLG; ATOD2
Reactivity
Human. Others not tested.
Applications
Immunohistochemistry
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of NRK cells using anti-IL1RA/Il1rn antibody (AAA125566).Overlay histogram showing NRK cells stained with AAA125566 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (AAA125566, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IL1RA/Il1rn, Polyclonal Antibody (Cat# AAA125566)
Full Name
Anti-IL1RA/Il1rn Antibody
Gene Names
Il1rn; IL-1ra; F630041P17Rik
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of CYP2E1 using anti-CYP2E1 antibody (AAA46078).CYP2E1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CYP2E1 Antibody (AAA46078) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cytochrome P450 2E1, Polyclonal Antibody (Cat# AAA46078)
Full Name
Anti-CYP2E1 Antibody
Gene Names
CYP2E1; CPE1; CYP2E; P450-J; P450C2E
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemisry)
(Figure 3. IHC analysis of CD40L using anti-CD40L antibody (AAA124621).CD40L was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD40L Antibody (AAA124621) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CD40L, Polyclonal Antibody (Cat# AAA124621)
Full Name
Anti-CD40L Picoband antibody
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of FLIP using anti-FLIP antibody.FLIP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-FLIP antibody.FLIP was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/mL rabbit anti-FLIP Antibody overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
FLIP/CFLAR, Polyclonal Antibody (Cat# AAA124923)
Full Name
Anti-FLIP/CFLAR Antibody
Gene Names
CFLAR; CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using BAP1 Mouse Monoclonal Antibody (BAP1/2431).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
BAP1 (BRCA1 Associated Protein 1), Monoclonal Antibody (Cat# AAA214842)
Full Name
BAP1 (BRCA1 Associated Protein 1)
Gene Names
BAP1; UCHL2; hucep-6; HUCEP-13
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using Fibronectin Mouse Monoclonal Antibody (FN1/2948). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Fibronectin, Monoclonal Antibody (Cat# AAA215365)
Full Name
Fibronectin
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of SiHa cells using anti-Lipin 1/LPIN1 antibody (AAA127120).Overlay histogram showing SiHa cells stained with AAA127120 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lipin 1/LPIN1 Antibody (AAA127120, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Lipin 1/LPIN1, Polyclonal Antibody (Cat# AAA127120)
Full Name
Anti-Lipin 1/LPIN1 Antibody Picoband
Gene Names
LPIN1; PAP1
Reactivity
Human, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of JK cells using anti-SLC38A9 antibody (AAA127331).Overlay histogram showing JK cells stained with AAA127331 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC38A9 Antibody (AAA127331, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
SLC38A9, Polyclonal Antibody (Cat# AAA127331)
Full Name
Anti-SLC38A9 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using NRF1 Mouse Monoclonal Antibody (NRF1/2608). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
NRF1, Monoclonal Antibody (Cat# AAA215112)
Full Name
NRF1
Gene Names
NRF1; ALPHA-PAL
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of THP-1 cells using anti-NOXA2/p67phox/NCF2 antibody (AAA125654).Overlay histogram showing THP-1 cells stained with AAA125654 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NOXA2/p67phox/NCF2 Antibody (AAA125654,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NOXA2/p67phox/NCF2, Polyclonal Antibody (Cat# AAA125654)
Full Name
Anti-NOXA2/p67phox/NCF2 Antibody
Gene Names
NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of Raji cells using anti-Integrin beta 5/ITGB5 antibody (AAA125718).Overlay histogram showing Raji cells stained with AAA125718 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin beta 5/ITGB5 Antibody (AAA125718,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Integrin beta 5/ITGB5, Polyclonal Antibody (Cat# AAA125718)
Full Name
Anti-Integrin beta 5/ITGB5 Antibody
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of METAP2 using anti-METAP2 antibody (AAA127192).METAP2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-METAP2 Antibody (AAA127192) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
METAP2, Polyclonal Antibody (Cat# AAA127192)
Full Name
Anti-METAP2 Antibody Picoband
Gene Names
METAP2; MAP2; MNPEP; p67eIF2
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Anti-Ferritin light chain rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:19 kDaObserved band size:19 kDa)
Ferritin light chain, Polyclonal Antibody (Cat# AAA258905)
Full Name
Anti-Ferritin light chain Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Pricing
ICC (Immunocytochemistry)
(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-SMC1A mouse mAb(dilution 1:100).)
SMC1A, Monoclonal Antibody (Cat# AAA320307)
Full Name
SMC1A (C-term) Mouse mAb
Gene Names
SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Pricing