Search results for "Recombinant Proteins"

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WB (Western Blot) (Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Porcine Cerebrum lysate; Lane3: Rat Placenta lysate Primary Ab: 3ug/ml Mouse AntiHuman VGF Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

VGF Nerve Growth Factor Inducible (VGF), Monoclonal Antibody (Cat# AAA151684)

Full Name

Monoclonal Antibody to VGF Nerve Growth Factor Inducible (VGF)

Reactivity

Human, Rat, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Tryptase Mouse Monoclonal Antibody (TPSAB1/1961).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Tryptase, Monoclonal Antibody (Cat# AAA214704)

Full Name

Tryptase (Mast Cell Marker)

Gene Names

TPSAB1; TPS1; TPS2; TPSB1

Reactivity

Human. Others not known.

Applications

Immunohistochemistry

Pricing

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Caspase 9 (CASP9), Monoclonal Antibody (Cat# AAA151587)

Full Name

Monoclonal Antibody to Caspase 9 (CASP9)

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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Bioactivity (GSTa1 (Glutathione S-transferase a1) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant human GSTa1, mix gentliy. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant human GSTa1 is >17000 pmol/min/ug.)

Glutathione S Transferase Alpha 1 (GSTa1), Active Protein (Cat# AAA161754)

Full Name

Active Glutathione S Transferase Alpha 1 (GSTa1)

Gene Names

GSTA1; GST2; GTH1; GSTA1-1

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

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Bioactivity (Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth. The activity of recombinant human CA3 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA3 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)

Carbonic Anhydrase III, Muscle Specific (CA3), Active Protein (Cat# AAA161899)

Full Name

Active Carbonic Anhydrase III, Muscle Specific (CA3)

Gene Names

CA3; Car3; CAIII

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using Smad2 mouse mAb (dilution 1:100).)

Smad2, Monoclonal Antibody (Cat# AAA320359)

Full Name

Smad2 Mouse mAb

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human

Applications

Immunocytochemistry, Western Blot

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Pricing

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of JK cells using anti-CD2 antibody (AAA126043).Overlay histogram showing JK cells stained with AAA126043 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD2 Antibody (AAA126043, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CD2, Polyclonal Antibody (Cat# AAA126043)

Full Name

Anti-CD2 Antibody Picoband

Gene Names

CD2; T11; SRBC; LFA-2

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 14. Flow Cytometry analysis of Jurkat cells using anti-SUCLG2 antibody (AAA19901).Overlay histogram showing Jurkat cells stained with AAA19901 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SUCLG2 Antibody (AAA19901, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of SUCLG2 using anti-SUCLG2 antibody (AAA19901).SUCLG2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SUCLG2 Antibody (AAA19901) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hela whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human COLO320 whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG2 antigen affinity purified polyclonal antibody (#AAA19901) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SUCLG2 at approximately 47 kDa. The expected band size for SUCLG2 is at 47 kDa.)

SUCLG2, Polyclonal Antibody (Cat# AAA19901)

Full Name

Anti-SUCLG2 Antibody Picoband

Gene Names

SUCLG2; GBETA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-TRBP/TARBP2 antibody (AAA19428).Overlay histogram showing 293T cells stained with AAA19428 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRBP/TARBP2 Antibody (AAA19428, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TRBP/TARBP2, Polyclonal Antibody (Cat# AAA19428)

Full Name

Anti-TRBP/TARBP2 Antibody Picoband

Gene Names

TARBP2; LOQS; TRBP; TRBP1; TRBP2

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

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ELISA (Figure 2 ELISA Validation with SARS-CoV-2 (COVID-19) Spike RBD, S1 and ECD Proteins Antibodies: SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40980. A direct ELISA was performed using SARS-CoV-2 Spike recombinant proteins (RBD, 10-303; S1, 97-087; ECD, 11-070) as coating antigen at 1ug/mL and the anti-SARS-CoV-2 (COVID-19) Spike S1 RBD antibody (AAA40980) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1ug/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL. SARS-CoV-2 (COVID-19) Spike S1 RBD Antibody, AAA40980 can detect spike RBD, S1 and ECD proteins at 2 ng/mL.)

SARS-CoV-2 (COVID-19) S1 RBD, Monoclonal Recombinant Antibody (Cat# AAA40980)

Full Name

SARS-CoV-2 (COVID-19) S1 RBD Single Domain Antibody [T5P7-G10]

Reactivity

Virus

Applications

ELISA

Purity

SARS-CoV-2 (COVID-19) S1 RBD Antibody [T5P7-G10] is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.

Pricing

IL-1alpha, CLIA Kit (Cat# AAA318538)

Full Name

IL-1alpha (Human) LumiAb ELISA Kit

Gene Names

IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA

Reactivity

Human

Pricing

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Claudin 6 (CLDN6), Recombinant Protein (Cat# AAA153736)

Full Name

Recombinant Claudin 6 (CLDN6)

Applications

Western Blot

Purity

Greater than 95% by SDS-PAGE

Pricing

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Choline Acetyltransferase (ChAT), Recombinant Protein (Cat# AAA153494)

Full Name

Recombinant Choline Acetyltransferase (ChAT)

Gene Names

Chat; B230380D24Rik

Reactivity

Mus musculus (Mouse)

Applications

Western Blot

Purity

> 95%

Pricing

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Aprotinin (AP), Recombinant Protein (Cat# AAA153412)

Full Name

Recombinant Aprotinin (AP)

Applications

Western Blot

Purity

Greater than 95% by SDS-PAGE

Pricing

Adiponectin, ELISA Kit (Cat# AAA105672)

Full Name

Human Adiponectin ELISA Kit

Gene Names

ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1

Reactivity

Human

Applications

Sandwich ELISA

Pricing

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Transferrin (TF), Recombinant Protein (Cat# AAA153510)

Full Name

Recombinant Transferrin (TF)

Gene Names

TF; TFQTL1; PRO1557; PRO2086

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

Total MMP-8, ELISA Kit (Cat# AAA315761)

Full Name

Total MMP-8 (Human) OmniKine ELISA Kit

Gene Names

MMP8; HNC; CLG1; MMP-8; PMNL-CL

Reactivity

Human

Pricing

IFN-gamma, ELISA Kit (Cat# AAA315667)

Full Name

IFN-gamma (Murine) OmniKine ELISA Kit

Gene Names

Ifng; Ifg; IFN-g

Reactivity

Mouse

Pricing

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Bioactivity (HTRF assay for RPS6KA1/RSK1 activity 1 uM STK S1 substrate was incubated with different concentrations of RPS6KA1/RSK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 30 min. All operations and reactions were performed at room temperature, and HTRF assay was used to detect the enzymatic activity.)

RPS6KA1/RSK1, Active Protein (Cat# AAA60316)

Full Name

Recombinant RPS6KA1/RSK1 protein

Purity

>=90%

Pricing

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Myelin Basic Protein (MBP), Recombinant Protein (Cat# AAA161962)

Full Name

Recombinant Myelin Basic Protein (MBP)

Gene Names

Mbp; Mbps

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using DRD3 antibody. Blue: DAPI for nuclear staining.)

DRD3, Polyclonal Antibody (Cat# AAA37560)

Full Name

DRD3 Polyclonal Antibody

Gene Names

DRD3; D3DR; ETM1; FET1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of PTGS2 using anti-PTGS2 antibody.)

COX2/Cyclooxygenase 2/PTGS2, Polyclonal Antibody (Cat# AAA125390)

Full Name

Anti-COX2/Cyclooxygenase 2/PTGS2 Antibody

Gene Names

PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS

Reactivity

Human

Applications

Direct ELISA, Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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Fibrillin 2 (FBN2), Recombinant Protein (Cat# AAA150669)

Full Name

Recombinant Fibrillin 2 (FBN2)

Gene Names

FBN2; CCA; DA9

Reactivity

Homo sapiens (Human)

Applications

Western Blot

Purity

> 95%

Pricing

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Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), Recombinant Protein (Cat# AAA152300)

Full Name

Recombinant Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A)

Applications

Western Blot

Purity

Greater than 97% by SDS-PAGE

Pricing

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Actin Alpha 2, Smooth Muscle (ACTa2), Recombinant Protein (Cat# AAA153447)

Full Name

Recombinant Actin Alpha 2, Smooth Muscle (ACTa2)

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

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Fibroblast Growth Factor Receptor 1 (FGFR1), Recombinant Protein (Cat# AAA153478)

Full Name

Recombinant Fibroblast Growth Factor Receptor 1 (FGFR1)

Gene Names

Fgfr1; FLG; MFR; Eask; Hspy; Flt-2; c-fgr; Fgfr-1; AW208770; bFGF-R-1

Applications

Western Blot

Purity

Greater than 95% by SDS-PAGE

Pricing

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Superoxide Dismutase 3, Extracellular (SOD3), Recombinant Protein (Cat# AAA152048)

Full Name

Eukaryotic Superoxide Dismutase 3, Extracellular (SOD3)

Gene Names

Sod3; EC-SOD; AI314465

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

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CD22, Recombinant Protein (Cat# AAA120539)

Full Name

Recombinant Human CD22, C-Fc

Gene Names

CD22; SIGLEC2; SIGLEC-2

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

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C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3), Recombinant Protein (Cat# AAA153693)

Full Name

Recombinant C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3)

Gene Names

C1qtnf3; Cors; CTRP3; Corcs; CORS26; CORS-26; 2310005P21Rik

Applications

Western Blot

Purity

Greater than 90% by SDS-PAGE

Pricing

Estrogen Receptor a, Recombinant Protein (Cat# AAA60203)

Full Name

Recombinant Estrogen Receptor a protein

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Pricing

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NRG1/Neuregulin-1, Recombinant Protein (Cat# AAA120526)

Full Name

Recombinant Human NRG1/Neuregulin-1, C-Fc

Gene Names

NRG1; GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131

Applications

Western Blot

Purity

>90% as determined by SDS-PAGE

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colon cancer section using Pink1(Cat#AAA290950). AAA290950 was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

PINK1, Monoclonal Antibody (Cat# AAA290950)

Full Name

PINK1 Antibody

Gene Names

PINK1; BRPK; PARK6

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

This antibody is purified through a protein G column, followed by dialysis against PBS.

Pricing

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IF (Immunofluorescence) (ICC/IF analysis of SORD in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SORD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

SORD, Monoclonal Antibody (Cat# AAA48127)

Full Name

SORD antibody

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Flow Cytometry, Western Blot

Purity

By protein-A affinity chromatography

Pricing

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IHC (Immunohistochemisry) (Immunochemical staining of human IL6 in human liver with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)

IL-6, Monoclonal Antibody (Cat# AAA254896)

Full Name

Anti-IL-6 Antibody, Mouse Monoclonal

Gene Names

IL6; HGF; HSF; BSF2; IL-6; IFNB2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Protein A

Pricing

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IP (Immunoprecipitation) (Galectin3 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate2 uL anti-Galectin3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Galectin3 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)

Galectin 3/LGALS3, Monoclonal Antibody (Cat# AAA254797)

Full Name

Recombinant Anti-Galectin 3/LGALS3 Antibody, Rabbit Monoclonal

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Protein A

Pricing

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IHC (Immunohistochemistry) (Anti-RUNX1/AML1 Picoband antibody, AAA46058-4.JPGIHC(P): Mouse Intestine Tissue)

Runt-related transcription factor 1, Polyclonal Antibody (Cat# AAA46058)

Full Name

Anti-RUNX1/AML1 Antibody

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Reactivity

Human, Mouse, Rat. No cross reactivity with other proteins.

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

Nucleoporin, Recombinant Protein (Cat# AAA144411)

Full Name

Recombinant Nucleoporin 214kDa (NUP214)

Reactivity

Mus musculus (Mouse)

Applications

Western Blot

Purity

> 97%

Pricing

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Application Data (Recombinant Mononucleosomes H3.3 (K18I) DNA gel Recombinant Mononucleosomes H3.3 (K18I) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K18I). Intact mononucleosomes H3.3 (K18I) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)

Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60227)

Full Name

Recombinant Mononucleosomes H3.3 (K18I)

Pricing

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IF (Immunofluorescence) (ICC/IF analysis of C/EBP-beta in HaLa cells. The cell was stained with ABP0609 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

C/EBP-Beta, Monoclonal Antibody (Cat# AAA47986)

Full Name

C/EBP-Beta antibody

Gene Names

CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

By protein-G affinity chromatography

Pricing

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Hepatitis B virus HBSAG, Recombinant Protein (Cat# AAA47606)

Full Name

Hepatitis B virus HBSAG Protein, hFc Tag

Purity

Purified from cell culture supernatant by affinity chromatography
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Pricing

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Transglutaminase 2, Tissue (TGM2), Recombinant Protein (Cat# AAA134189)

Full Name

Recombinant Transglutaminase 2, Tissue (TGM2)

Gene Names

TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h

Applications

Western Blot

Purity

> 80%

Pricing

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ELISA (Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein ELISA DataImmobilized Human IL-15 at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human IL-15RA, His Tag with the EC50 of 18.1ng/ml determined by ELISA.)

IL-15RA/IL-15 R alpha/CD215, Recombinant Protein (Cat# AAA76249)

Full Name

Biotinylated Human IL-15RA/IL-15 R alpha/CD215 Protein Recombinant

Gene Names

IL15RA; CD215

Purity

>95%, as determined by SDS-PAGE and HPLC

Pricing

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Bioactivity (Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroidpotentiating and cell growth promoting activities. The activity of recombinant chicken TIMP2 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and rgTIMP2 (MW: 23 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of rgTIMP2 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant chicken TIMP2 significantly decreased rhMMP2 activity. The inhibition IC50 was )

Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161696)

Full Name

Active Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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Application Data

Sirtuin 3 (SIRT3), Active Protein (Cat# AAA148212)

Full Name

Active Sirtuin 3 (SIRT3)

Reactivity

Rat

Applications

Activity Assay, Cell Culture

Purity

> 95%

Pricing

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SDS-PAGE (Sample: Active recombinant LBP, Human)

Lipopolysaccharide Binding Protein, Active Protein (Cat# AAA21164)

Full Name

Active Lipopolysaccharide Binding Protein (LBP)

Gene Names

LBP; BPIFD2

Applications

Cell Culture, Activity Assay

Purity

>80%

Pricing

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ELISA (Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein ELISA DataImmobilized Biotinylated Human CD3E&CD3G, hFc Tag at 1?g/ml (100?l/Well) on streptavidin (5?g/ml) precoated plate. Dose response curve for OKT3, mFc Tag with the EC50 of 55.2ng/ml determined by ELISA.)

CD3E&CD3G/CD3 epsilon&CD3 gamma, Recombinant Protein (Cat# AAA76290)

Full Name

Biotinylated Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein Recombinant

Gene Names

CD3E; T3E; TCRE; IMD18

Purity

>95%, as determined by SDS-PAGE and HPLC

Pricing

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Bioactivity (C reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant dog CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant dog CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.82 ug/mL.)

C ReProtein (CRP), Active Protein (Cat# AAA161778)

Full Name

Active C Reactive Protein (CRP)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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C-Type Lectin Domain Family 2, Member C (CLEC2C), Recombinant Protein (Cat# AAA152134)

Full Name

Eukaryotic C-Type Lectin Domain Family 2, Member C (CLEC2C)

Gene Names

CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26

Applications

Western Blot

Purity

Greater than 95% by SDS-PAGE

Pricing

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Bioactivity (GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant rat GSTa3, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant rat GSTa3 is >37000 pmol/min/ug.)

Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161768)

Full Name

Active Glutathione S Transferase Alpha 3 (GSTa3)

Gene Names

Gsta1; Yc1; Gsta3; Gsta5; GST AA; GST 2-2; GST Yc1; GST A3-3

Applications

Activity Assay, Cell Culture

Purity

Greater than 97% by SDS-PAGE

Pricing

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Aquaporin 4 (AQP4), Monoclonal Antibody (Cat# AAA151580)

Full Name

Monoclonal Antibody to Aquaporin 4 (AQP4)

Gene Names

AQP4; MIWC; HMIWC2

Reactivity

Human

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Protein A + Protein G affinity chromatography

Pricing

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10000 results for "Recombinant Proteins" - showing 2850-2900

VGF Nerve Growth Factor Inducible (VGF), Monoclonal Antibody (Cat# AAA151684)

Tryptase, Monoclonal Antibody (Cat# AAA214704)

Caspase 9 (CASP9), Monoclonal Antibody (Cat# AAA151587)

Glutathione S Transferase Alpha 1 (GSTa1), Active Protein (Cat# AAA161754)

Carbonic Anhydrase III, Muscle Specific (CA3), Active Protein (Cat# AAA161899)

Smad2, Monoclonal Antibody (Cat# AAA320359)

CD2, Polyclonal Antibody (Cat# AAA126043)

SUCLG2, Polyclonal Antibody (Cat# AAA19901)

TRBP/TARBP2, Polyclonal Antibody (Cat# AAA19428)

SARS-CoV-2 (COVID-19) S1 RBD, Monoclonal Recombinant Antibody (Cat# AAA40980)

IL-1alpha, CLIA Kit (Cat# AAA318538)

Claudin 6 (CLDN6), Recombinant Protein (Cat# AAA153736)

Choline Acetyltransferase (ChAT), Recombinant Protein (Cat# AAA153494)

Aprotinin (AP), Recombinant Protein (Cat# AAA153412)

Adiponectin, ELISA Kit (Cat# AAA105672)

Transferrin (TF), Recombinant Protein (Cat# AAA153510)

Total MMP-8, ELISA Kit (Cat# AAA315761)

IFN-gamma, ELISA Kit (Cat# AAA315667)

RPS6KA1/RSK1, Active Protein (Cat# AAA60316)

Myelin Basic Protein (MBP), Recombinant Protein (Cat# AAA161962)

DRD3, Polyclonal Antibody (Cat# AAA37560)

COX2/Cyclooxygenase 2/PTGS2, Polyclonal Antibody (Cat# AAA125390)

Fibrillin 2 (FBN2), Recombinant Protein (Cat# AAA150669)

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A), Recombinant Protein (Cat# AAA152300)

Actin Alpha 2, Smooth Muscle (ACTa2), Recombinant Protein (Cat# AAA153447)

Fibroblast Growth Factor Receptor 1 (FGFR1), Recombinant Protein (Cat# AAA153478)

Superoxide Dismutase 3, Extracellular (SOD3), Recombinant Protein (Cat# AAA152048)

CD22, Recombinant Protein (Cat# AAA120539)

C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3), Recombinant Protein (Cat# AAA153693)

Estrogen Receptor a, Recombinant Protein (Cat# AAA60203)

NRG1/Neuregulin-1, Recombinant Protein (Cat# AAA120526)

PINK1, Monoclonal Antibody (Cat# AAA290950)

SORD, Monoclonal Antibody (Cat# AAA48127)

IL-6, Monoclonal Antibody (Cat# AAA254896)

Galectin 3/LGALS3, Monoclonal Antibody (Cat# AAA254797)

Runt-related transcription factor 1, Polyclonal Antibody (Cat# AAA46058)

Nucleoporin, Recombinant Protein (Cat# AAA144411)

Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60227)

C/EBP-Beta, Monoclonal Antibody (Cat# AAA47986)

Hepatitis B virus HBSAG, Recombinant Protein (Cat# AAA47606)

Transglutaminase 2, Tissue (TGM2), Recombinant Protein (Cat# AAA134189)

IL-15RA/IL-15 R alpha/CD215, Recombinant Protein (Cat# AAA76249)

Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), Active Protein (Cat# AAA161696)

Sirtuin 3 (SIRT3), Active Protein (Cat# AAA148212)

Lipopolysaccharide Binding Protein, Active Protein (Cat# AAA21164)

CD3E&CD3G/CD3 epsilon&CD3 gamma, Recombinant Protein (Cat# AAA76290)

C ReProtein (CRP), Active Protein (Cat# AAA161778)

C-Type Lectin Domain Family 2, Member C (CLEC2C), Recombinant Protein (Cat# AAA152134)

Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161768)

Aquaporin 4 (AQP4), Monoclonal Antibody (Cat# AAA151580)

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