Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online Need help? +1-800-604-9114 or contact us

Filters


Clonality

Type

Reactivity

Gene Name

Isotype

Host

Application

Clone

Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.


Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.


Viewing 7850-7900 of 96812 product results


product-image-AAA125703_FCM13.jpg FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of 293T cells using anti-CYP11B1/C11B2/CYP11B2 antibody (AAA125703).Overlay histogram showing 293T cells stained with AAA125703 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP11B1/C11B2/CYP11B2 Antibody (AAA125703,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CYP11B1/C11B2/CYP11B2, Polyclonal Antibody (Cat# AAA125703)

No reviews yet
Full Name
Anti-CYP11B1/C11B2/CYP11B2 Antibody
Gene Names
CYP11B1; FHI; CPN1; CYP11B; P450C11
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125723_WB13.jpg WB (Western Blot) (Figure 2. Western blot analysis of Claudin 3/CLDN3 using anti-Claudin 3/CLDN3 antibody (AAA125723).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat PC-12 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Claudin 3/CLDN3 antigen affinity purified polyclonal antibody (Catalog # AAA125723) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Claudin 3/CLDN3 at approximately 23KD. The expected band size for Claudin 3/CLDN3 is at 23KD.)

Claudin 3/CLDN3, Polyclonal Antibody (Cat# AAA125723)

No reviews yet
Full Name
Anti-Claudin 3/CLDN3 Antibody
Gene Names
Cldn3; mRVP1; Cpetr2; AI182374
Reactivity
Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125726_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U20S cells using anti-LXR beta/NER/NR1H2 antibody (AAA125726).Overlay histogram showing U20S cells stained with AAA125726 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LXR beta/NER/NR1H2 Antibody (AAA125726, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

LXR beta/NER/NR1H2, Polyclonal Antibody (Cat# AAA125726)

No reviews yet
Full Name
Anti-LXR beta/NER/NR1H2 Antibody
Gene Names
NR1H2; NER; UNR; LXRB; LXR-b; NER-I; RIP15
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125731_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Hela cells using anti-SLC22A3 antibody (AAA125731).Overlay histogram showing Hela cells stained with AAA125731 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC22A3 Antibody (AAA125731, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SLC22A3, Polyclonal Antibody (Cat# AAA125731)

No reviews yet
Full Name
Anti-SLC22A3 Antibody
Gene Names
SLC22A3; EMT; EMTH; OCT3
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125733_IHC8.jpg IHC (Immunohistochemistry) (Figure 5. IHC analysis of LOC134147/CMBL using anti-LOC134147/CMBL antibody (AAA125733).LOC134147/CMBL was detected in paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-LOC134147/CMBL Antibody (AAA125733) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

LOC134147/CMBL, Polyclonal Antibody (Cat# AAA125733)

No reviews yet
Full Name
Anti-LOC134147/CMBL Antibody
Gene Names
CMBL; JS-1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125737_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U20S cells using anti-Kir6. 1/KCNJ8 antibody (AAA125737).Overlay histogram showing U20S cells stained with AAA125737 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kir6. 1/KCNJ8 Antibody (AAA125737, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Kir6. 1/KCNJ8, Polyclonal Antibody (Cat# AAA125737)

No reviews yet
Full Name
Anti-Kir6. 1/KCNJ8 Antibody
Gene Names
KCNJ8; KIR6.1; uKATP-1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125738_IF13.jpg IF (Immunofluorescence) (Figure 2. IF analysis of ACADS/SCAD using anti-ACADS/SCAD antibody (AAA125738).ACADS/SCAD was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ACADS/SCAD Antibody (AAA125738) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

ACADS/SCAD, Polyclonal Antibody (Cat# AAA125738)

No reviews yet
Full Name
Anti-ACADS/SCAD Antibody
Gene Names
ACADS; SCAD; ACAD3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125741_IF10.jpg IF (Immunofluorescence) (Figure 4. IF analysis of CTHRC1 using anti-CTHRC1 antibody (AAA125741).CTHRC1 was detected in immunocytochemical section of HPEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-CTHRC1 Antibody (AAA125741) overnight at 4 degree C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

CTHRC1, Polyclonal Antibody (Cat# AAA125741)

No reviews yet
Full Name
Anti-CTHRC1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125748_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of Hela cells using anti-Tubulin beta antibody (AAA125748).Overlay histogram showing Hela cells stained with AAA125748 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tubulin beta Antibody (AAA125748,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Tubulin beta, Polyclonal Antibody (Cat# AAA125748)

No reviews yet
Full Name
Anti-Tubulin beta Antibody
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125756_FACS8.png FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of HEPA1-6 cells using anti-GILT/IFI30 antibody (AAA125756).Overlay histogram showing HEPA1-6 cells stained with AAA125756 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GILT/IFI30 Antibody (AAA125756, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

GILT/IFI30, Polyclonal Antibody (Cat# AAA125756)

No reviews yet
Full Name
Anti-GILT/IFI30 Antibody
Gene Names
Ifi30; GILT; IP30
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125762_FCM13.jpg FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of SiHa cells using anti-SEC23B antibody (AAA125762).Overlay histogram showing SiHa cells stained with AAA125762 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC23B Antibody (AAA125762, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SEC23B, Polyclonal Antibody (Cat# AAA125762)

No reviews yet
Full Name
Anti-SEC23B Antibody
Gene Names
SEC23B; CDAII; CDAN2; CDA-II; HEMPAS
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125770_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of PC-3 cells using anti-K Cadherin/CDH6 antibody (AAA125770).Overlay histogram showing PC-3 cells stained with AAA125770 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-K Cadherin/CDH6 Antibody (AAA125770, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

K Cadherin/CDH6, Polyclonal Antibody (Cat# AAA125770)

No reviews yet
Full Name
Anti-K Cadherin/CDH6 Antibody
Gene Names
CDH6; CAD6; KCAD
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125771_IF10.jpg IF (Immunofluorescence) (Figure 4. IF analysis of PTCH2 using anti- PTCH2 antibody (AAA125771).PTCH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-PTCH2 Antibody (AAA125771) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

PTCH2, Polyclonal Antibody (Cat# AAA125771)

No reviews yet
Full Name
Anti-PTCH2 Antibody
Gene Names
PTCH2; PTC2
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125772_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-MT2-MMP/MMP15 antibody (AAA125772).Overlay histogram showing CACO-2 cells stained with AAA125772 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MT2-MMP/MMP15 Antibody (AAA125772, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MT2-MMP/MMP15, Polyclonal Antibody (Cat# AAA125772)

No reviews yet
Full Name
Anti-MT2-MMP/MMP15 Antibody
Gene Names
MMP15; MTMMP2; SMCP-2; MT2-MMP
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125780_FACS8.jpg FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of SiHa cells using anti-MIEF1 antibody (AAA125780).Overlay histogram showing SiHa cells stained with AAA125780 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIEF1 Antibody (AAA125780, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MIEF1, Polyclonal Antibody (Cat# AAA125780)

No reviews yet
Full Name
Anti-MIEF1 Antibody
Gene Names
MIEF1; MID51; SMCR7L; HSU79252; dJ1104E15.3
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125782_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A431 cells using anti-DRAGON/RGMB antibody (AAA125782).Overlay histogram showing A431 cells stained with AAA125782 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRAGON/RGMB Antibody (AAA125782, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

DRAGON/RGMB, Polyclonal Antibody (Cat# AAA125782)

No reviews yet
Full Name
Anti-DRAGON/RGMB Antibody
Gene Names
RGMB; DRAGON
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125788_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of A549 cells using anti-MMP28 antibody (AAA125788).Overlay histogram showing A549 cells stained with AAA125788 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MMP28 Antibody (AAA125788, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MMP28, Polyclonal Antibody (Cat# AAA125788)

No reviews yet
Full Name
Anti-MMP28 Antibody
Gene Names
MMP28; MM28; MMP25; MMP-28; EPILYSIN
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125789_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U251 cells using anti-Grainyhead-like protein 1/GRHL1 antibody (AAA125789).Overlay histogram showing U251 cells stained with AAA125789 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Grainyhead-like protein 1/GRHL1 Antibody (AAA125789, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Grainyhead-like protein 1/GRHL1, Polyclonal Antibody (Cat# AAA125789)

No reviews yet
Full Name
Anti-Grainyhead-like protein 1/GRHL1 Antibody
Gene Names
GRHL1; MGR; NH32; LBP32; TFCP2L2
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125794_FACS8.jpg FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of U87 cells using anti-Arginine Decarboxylase/AZIN2 antibody (AAA125794).Overlay histogram showing U87 cells stained with AAA125794 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Arginine Decarboxylase/AZIN2 Antibody (AAA125794, 1 μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1 x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Arginine decarboxylase/AZIN2, Polyclonal Antibody (Cat# AAA125794)

No reviews yet
Full Name
Anti-Arginine decarboxylase/AZIN2 Antibody
Gene Names
AZIN2; ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125798_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U251 cells using anti-HOXC13 antibody (AAA125798).Overlay histogram showing U251 cells stained with AAA125798 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXC13 Antibody (AAA125798, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

HOXC13, Polyclonal Antibody (Cat# AAA125798)

No reviews yet
Full Name
Anti-HOXC13 Antibody
Gene Names
HOXC13; HOX3; ECTD9; HOX3G
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125803_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of ALDH6A1 using anti-ALDH6A1 antibody (AAA125803).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysatesLane 2: rat heart tissue lysatesLane 3: rat kidney tissue lysatesLane 4: mouse liver tissue lysatesLane 5: mouse kidney tissue lysatesLane 6: mouse NIH/3T3 whole cell lysatesLane 7: human MCF-7 whole cell lysatesLane 8: human HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ALDH6A1 antigen affinity purified polyclonal antibody (Catalog # AAA125803) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALDH6A1 at approximately 58KD. The expected band size for ALDH6A1 is at 58KD.)

ALDH6A1, Polyclonal Antibody (Cat# AAA125803)

No reviews yet
Full Name
Anti-ALDH6A1 Antibody
Gene Names
ALDH6A1; MMSDH; MMSADHA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125814_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U937 cells using anti-RGS18 antibody (AAA125814).Overlay histogram showing U937 cells stained with AAA125814 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS18 Antibody (AAA125814, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

RGS18, Polyclonal Antibody (Cat# AAA125814)

No reviews yet
Full Name
Anti-RGS18 Antibody
Gene Names
RGS18; RGS13
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125824_IF13.jpg IF (Immunofluorescence) (Figure 2. IF analysis of PHF21B using anti- PHF21B antibody (AAA125824).PHF21B was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- PHF21B Antibody (AAA125824) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

PHF21B, Polyclonal Antibody (Cat# AAA125824)

No reviews yet
Full Name
Anti-PHF21B Antibody
Gene Names
PHF21B; PHF4; BHC80L
Reactivity
Human
Applications
Direct ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125830_IHC10.jpg IHC (Immunohistochemistry) (Figure 4. IHC analysis of LY6C using anti-LY6C antibody (AAA125830).LY6C was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LY6C Antibody (AAA125830) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

LY6C, Polyclonal Antibody (Cat# AAA125830)

No reviews yet
Full Name
Anti-LY6C Antibody
Gene Names
Ly6c1; Ly6c; Ly-6C; Ly-6C1; AA682074; AA959465
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing

GLUT9/SLC2A9, Polyclonal Antibody (Cat# AAA125447)

No reviews yet
Full Name
Anti-GLUT9/SLC2A9 Antibody
Gene Names
SLC2A9; GLUT9; GLUTX; UAQTL2; URATv1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125455_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of CBR1 using anti-CBR1 antibody.)

CBR1, Polyclonal Antibody (Cat# AAA125455)

No reviews yet
Full Name
Anti-CBR1 Antibody
Gene Names
CBR1; CBR; hCBR1; SDR21C1
Reactivity
Human
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125456_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of FOXE1 using anti-FOXE1 antibody.)

FOXE1, Polyclonal Antibody (Cat# AAA125456)

No reviews yet
Full Name
Anti-FOXE1 Antibody
Gene Names
FOXE1; TTF2; FOXE2; HFKH4; HFKL5; NMTC4; TITF2; TTF-2; FKHL15
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125457_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of PPM1A using anti-PPM1A antibody.)

PPM1A, Polyclonal Antibody (Cat# AAA125457)

No reviews yet
Full Name
Anti-PPM1A Antibody
Gene Names
PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
Reactivity
Human
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125461_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of PLSCR1 using anti-PLSCR1 antibody.)

Scramblase 1/PLSCR1, Polyclonal Antibody (Cat# AAA125461)

No reviews yet
Full Name
Anti-Scramblase 1/PLSCR1 Antibody
Gene Names
PLSCR1; MMTRA1B
Reactivity
Human, Mouse
Applications
Direct ELISA, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125463_WB10.jpg WB (Western Blot) (Figure 1. Western blot analysis of MTA2 using anti-MTA2 antibody.)

MTA2/PID, Polyclonal Antibody (Cat# AAA125463)

No reviews yet
Full Name
Anti-MTA2/PID Antibody
Gene Names
MTA2; PID; MTA1L1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125469_WB11.jpg WB (Western Blot)

Acetyl Coenzyme A Carboxylase/ACACB, Polyclonal Antibody (Cat# AAA125469)

No reviews yet
Full Name
Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody
Gene Names
ACACB; ACC2; ACCB; HACC275
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125471_WB10.jpg WB (Western Blot) (Figure 1. Western blot analysis of ASIC2 using anti-ASIC2 antibody.)

ACCN1/ASIC2, Polyclonal Antibody (Cat# AAA125471)

No reviews yet
Full Name
Anti-ACCN1/ASIC2 Antibody
Gene Names
ASIC2; ACCN; BNC1; MDEG; ACCN1; BNaC1; ASIC2a; hBNaC1
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125472_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of CHD2 using anti-CHD2 antibody.)

CHD2, Polyclonal Antibody (Cat# AAA125472)

No reviews yet
Full Name
Anti-CHD2 Antibody
Gene Names
CHD2; EEOC
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125473_WB10.jpg WB (Western Blot) (Figure 1. Western blot analysis of CHRM1 using anti-CHRM1 antibody.)

Muscarinic Acetylcholine Receptor 1/CHRM1, Polyclonal Antibody (Cat# AAA125473)

No reviews yet
Full Name
Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 Antibody
Gene Names
CHRM1; M1; HM1; M1R
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125476_WB10.jpg WB (Western Blot)

SMC4, Polyclonal Antibody (Cat# AAA125476)

No reviews yet
Full Name
Anti-SMC4 Antibody
Gene Names
SMC4; CAPC; CAP-C; SMC-4; SMC4L1
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125509_FCM11.png FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of rat spleen tissue using anti-IL10 antibody (AAA125509).Overlay histogram showing rat spleen tissue stained with AAA125509 (Blue line). The tissue section was blocked with 10% normal goat serum. And then incubated with rabbit anti-IL10 Antibody (AAA125509, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IL10, Polyclonal Antibody (Cat# AAA125509)

No reviews yet
Full Name
Anti-IL10 Antibody
Gene Names
Il10; IL10X
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125510_IF10.jpg IF (Immunofluorescence) (Figure 4. IF analysis of EGFR using anti- EGFR antibody (AAA125510).EGFR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-EGFR Antibody (AAA125510) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

EGFR, Polyclonal Antibody (Cat# AAA125510)

No reviews yet
Full Name
Anti-EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125515_FCM13.png FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of mouse spleen tissues using anti-Caspase-9 p35/Casp9 antibody (AAA125515).Overlay histogram showing mouse spleen tissues stained with AAA125515 (Blue line). The tissues were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-9 p35/Casp9 Antibody (AAA125515, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Caspase-9 p35/Casp9, Polyclonal Antibody (Cat# AAA125515)

No reviews yet
Full Name
Anti-Caspase-9 p35/Casp9 Antibody
Gene Names
Casp9; Mch6; APAF-3; CASP-9; AI115399; AW493809; ICE-LAP6; Caspase-9
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125388_WB8.jpg WB (Western Blot)

Neurofibromin/NF1, Polyclonal Antibody (Cat# AAA125388)

No reviews yet
Full Name
Anti-Neurofibromin/NF1 Antibody
Gene Names
NF1; WSS; NFNS; VRNF
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125395_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of AXL using anti-AXL antibody.)

AXL, Polyclonal Antibody (Cat# AAA125395)

No reviews yet
Full Name
Anti-AXL Antibody
Gene Names
AXL; ARK; UFO; JTK11; Tyro7
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125396_WB13.jpg WB (Western Blot)

CD1D, Polyclonal Antibody (Cat# AAA125396)

No reviews yet
Full Name
Anti-CD1D Antibody
Gene Names
CD1D; R3; CD1A; R3G1
Reactivity
Human, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125398_WB10.jpg WB (Western Blot) (Figure 1. Western blot analysis of FLI1 using anti-FLI1 antibody.)

FLI1, Polyclonal Antibody (Cat# AAA125398)

No reviews yet
Full Name
Anti-FLI1 Antibody
Gene Names
FLI1; EWSR2; SIC-1; BDPLT21
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125408_WB11.jpg WB (Western Blot) (Figure 1. Western blot analysis of Olr1 using anti-Olr1 antibody.)

LOX 1/Olr1, Polyclonal Antibody (Cat# AAA125408)

No reviews yet
Full Name
Anti-LOX 1/Olr1 Antibody
Gene Names
Olr1; LOX-1; Oldr1; Oldlr1
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125409_WB13.jpg WB (Western Blot) (Figure 1. Western blot analysis of RBPJ using anti-RBPJ antibody.)

RBPJK/RBPJ, Polyclonal Antibody (Cat# AAA125409)

No reviews yet
Full Name
Anti-RBPJK/RBPJ Antibody
Gene Names
RBPJ; SUH; csl; AOS3; CBF1; KBF2; CBF-1; RBP-J; RBPJK; IGKJRB; RBP-JK; RBPSUH; IGKJRB1; RBP-J kappa
Reactivity
Human, Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125410_WB10.jpg WB (Western Blot) (Figure 1. Western blot analysis of FGA using anti-FGA antibody.)

Fibrinogen alpha chain/FGA, Polyclonal Antibody (Cat# AAA125410)

No reviews yet
Full Name
Anti-Fibrinogen alpha chain/FGA Antibody
Gene Names
FGA; Fib2
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125420_WB11.jpg WB (Western Blot) (Figure 1. Western blot analysis of Tnfrsf8 using anti-Tnfrsf8 antibody.)

CD30/Tnfrsf8, Polyclonal Antibody (Cat# AAA125420)

No reviews yet
Full Name
Anti-CD30/Tnfrsf8 Antibody
Gene Names
Tnfrsf8; Ki; Cd30; Ki-1; D1S166E
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125421_WB8.jpg WB (Western Blot)

FHL, Polyclonal Antibody (Cat# AAA125421)

No reviews yet
Full Name
Anti-FHL Antibody
Gene Names
FHL1; KYOT; SLIM; FCMSU; FHL-1; FHL1A; FHL1B; FLH1A; SLIM1; XMPMA; RBMX1A; RBMX1B; SLIM-1; SLIMMER
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125422_WB13.jpg WB (Western Blot)

FHL, Polyclonal Antibody (Cat# AAA125422)

No reviews yet
Full Name
Anti-FHL Antibody
Gene Names
Fhl1; KyoT; SLIM; FHL-1; SLIM-1; RAM14-1
Reactivity
Mouse, Rat
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125423_WB11.jpg WB (Western Blot) (Figure 1. Western blot analysis of KDM6B using anti-KDM6B antibody.)

KDM6B/JMJD3, Polyclonal Antibody (Cat# AAA125423)

No reviews yet
Full Name
Anti-KDM6B/JMJD3 Antibody
Gene Names
KDM6B; JMJD3; NEDCFSA
Reactivity
Human, Mouse
Applications
Direct ELISA, Flow Cytometry, Western Blot
Purity
Immunogen affinity purified.
Pricing
product-image-AAA125425_WB8.jpg WB (Western Blot) (Figure 1. Western blot analysis of CARD9 using anti-CARD9 antibody.)

CARD9, Polyclonal Antibody (Cat# AAA125425)

No reviews yet
Full Name
Anti-CARD9 Antibody
Gene Names
CARD9; CANDF2; hCARD9
Reactivity
Human
Applications
Direct ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing

What are Polyclonal Antibodies?


Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. This ability to bind multiple epitopes is what makes polyclonal antibodies highly sensitive, as explained in our detailed guide on polyclonal antibodies and why they matter.


As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).


Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.


Key Uses of Polyclonal Antibodies


  • Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

  • Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

  • ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

  • Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

  • Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.


Why Buy Polyclonal Antibodies from AAA Biotech?


1. Ideal for Various Applications


Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.


2. Rigorous Quality Control


All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.


3. Wide Assortment of Antibodies


Antibodies in our catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.


4. Highly Purified


Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.


FAQ


1. How are polyclonal antibodies produced?


Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.


2. How do polyclonal antibodies differ from monoclonal antibodies?


Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.


3. How should I store polyclonal antibodies?


Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.


Submit a Question

Please complete the form below and a representative will contact you as soon as possible.

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.