Polyclonal Antibodies

At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cell using TMPO antibody. Blue: DAPI for nuclear staining.)

TMPO, Polyclonal Antibody (Cat# AAA29699)

Full Name

TMPO Antibody

Gene Names

TMPO; TP; LAP2; CMD1T; LEMD4; PRO0868

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Akt, Polyclonal Antibody (Cat# AAA28964)

Full Name

Akt Polyclonal Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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beta Catenin, Polyclonal Antibody (Cat# AAA29797)

Full Name

beta Catenin Polyclonal Antibody

Gene Names

CTNNB1; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IP

Purity

Affinity purification

Pricing

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p53, Polyclonal Antibody (Cat# AAA31354)

Full Name

Acetyl-p53 (Lys373) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)

Applications

WB, IHC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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TARDBP, Polyclonal Antibody (Cat# AAA30659)

Full Name

TARDBP Rabbit Polyclonal Antibody

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HepG2 cells using anti-PFKFB1 antibody (AAA19903).Overlay histogram showing HepG2 cells stained with AAA19903 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFKFB1 Antibody (AAA19903, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PFKFB1 using anti-PFKFB1 antibody (AAA19903).PFKFB1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKFB1 Antibody (AAA19903) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKFB1 Antibody (AAA19903) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKFB1 Antibody (AAA19903) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PFKFB1 Antibody (AAA19903) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKFB1 antigen affinity purified polyclonal antibody (#AAA19903) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (55 kDa.)

PFKFB1, Polyclonal Antibody (Cat# AAA19903)

Full Name

Anti-PFKFB1 Antibody Picoband

Gene Names

PFKFB1; F6PK; HL2K; PFRX

Reactivity

Human

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

MEK-1/2, Polyclonal Antibody (Cat# AAA29065)

Full Name

MEK-1/2 Polyclonal Antibody

Gene Names

MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, IF

Pricing

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HNRNPA1, Polyclonal Antibody (Cat# AAA29846)

Full Name

HNRNPA1 Polyclonal Antibody

Gene Names

HNRNPA1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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eIF-4E, Polyclonal Antibody (Cat# AAA29880)

Full Name

eIF-4E Antibody

Gene Names

EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.)

NADPH Oxidase 4, Polyclonal Antibody (Cat# AAA29878)

Full Name

NADPH Oxidase 4 Antibody

Gene Names

NOX4; KOX; KOX-1; RENOX

Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, FC/FACS

Purity

Peptide affinity purified

Pricing

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p53, Polyclonal Antibody (Cat# AAA31429)

Full Name

Phospho-p53 (Ser392) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse
Predicted Reactivity: Pig (88%), Bovine (88%), Sheep (88%), Rabbit (88%)

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CEBPG antibody.)

CEBPG, Polyclonal Antibody (Cat# AAA28103)

Full Name

CEBPG Polyclonal Antibody

Gene Names

CEBPG; GPE1BP; IG/EBP-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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TOMM20, Polyclonal Antibody (Cat# AAA28123)

Full Name

TOMM20 Polyclonal Antibody

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity Purification

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HeLa cells using anti-delta 1 Catenin/CAS/CTNND1 antibody (AAA19453).Overlay histogram showing HeLa cells stained with AAA19453 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-delta 1 Catenin/CAS/CTNND1 Antibody (AAA19453, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

delta 1 Catenin/CAS/CTNND1, Polyclonal Antibody (Cat# AAA19453)

Full Name

Anti-delta 1 Catenin/CAS/CTNND1 Antibody Picoband

Gene Names

CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3 ug PIK3C2A Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PIK3C2A Polyclonal Antibody at a dilution of 1:1000.)

PIK3C2A, Polyclonal Antibody (Cat# AAA22280)

Full Name

PIK3C2A Polyclonal Antibody

Gene Names

PIK3C2A; CPK; PI3-K-C2A; PI3-K-C2(ALPHA)

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Stat-1alpha/beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

Stat-1 alpha/beta, Polyclonal Antibody (Cat# AAA29957)

Full Name

Stat-1 alpha/beta Antibody

Gene Names

STAT1; CANDF7; ISGF-3; STAT91

Reactivity

Human, Mouse

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using UMPS antibody.)

UMPS, Polyclonal Antibody (Cat# AAA28172)

Full Name

UMPS Polyclonal Antibody

Gene Names

UMPS; OPRT

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Figure 7: Immunohistochemistry Validation of AIFM2 in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AIFM2 antibody (AAA11045) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

AIFM2, Polyclonal Antibody (Cat# AAA11045)

Full Name

AIFM2 (IN) Antibody

Gene Names

AIFM2; AMID; PRG3; RP11-367H5.2

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB, IF

Purity

AIFM2 Antibody is affinity chromatography purified via peptide column.

Pricing

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Caspase3, Polyclonal Antibody (Cat# AAA29796)

Full Name

Caspase3 Polyclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using CDA Antibody at dilution of 1:100 (40x lens).)

CDA, Polyclonal Antibody (Cat# AAA28268)

Full Name

CDA Polyclonal Antibody

Gene Names

CDA; CDD

Reactivity

Human, Mouse

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using DYNLL1 Rabbit pAb (AAA28275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

DYNLL1, Polyclonal Antibody (Cat# AAA28275)

Full Name

DYNLL1 Polyclonal Antibody

Gene Names

DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

p300, Polyclonal Antibody (Cat# AAA29317)

Full Name

p300 Polyclonal Antibody

Gene Names

EP300; p300; KAT3B; RSTS2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

HBcAg (HBV), Polyclonal Antibody (Cat# AAA13719)

Full Name

Anti-HBcAg (HBV), rabbit IgG

Applications

WB, EIA

Purity

Immunoaffinity chromatography

Pricing

vvhA, Polyclonal Antibody (Cat# AAA27021)

Full Name

vvhA Antibody

Reactivity

Vibrio Vulnificus

Applications

EIA, WB

Purity

>95%
Protein G Purified

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

NY-CO-9, Polyclonal Antibody (Cat# AAA29084)

Full Name

NY-CO-9 Polyclonal Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

ERK1/ERK2, Polyclonal Antibody (Cat# AAA22198)

Full Name

ERK1/ERK2 Polyclonal Antibody

Gene Names

MAPK1; ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk

Reactivity

Human, Mouse, Rat

Applications

IHC, IF

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of A549 cell using BCL2 antibody. Blue: DAPI for nuclear staining.)

BCL2, Polyclonal Antibody (Cat# AAA29670)

Full Name

BCL2 Antibody

Reactivity

Human

Applications

WB, IHC, IF

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U20S cells using anti-VDAC3 antibody (AAA19301).Overlay histogram showing U20S cells stained with AAA19301 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDAC3 Antibody (AAA19301, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

VDAC3, Polyclonal Antibody (Cat# AAA19301)

Full Name

Anti-VDAC3 Antibody

Gene Names

VDAC3; VDAC-3; HD-VDAC3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IDH1, Polyclonal Antibody (Cat# AAA28676)

Full Name

IDH1 Antibody (Center)

Gene Names

IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26

Reactivity

Human, mouse (Predicted Reactivity: Bovine, Rat)

Applications

EIA, IHC, IF, WB, FC/FACS

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of 293T cells using anti-PTCD1 antibody (AAA19983).Overlay histogram showing 293T cells stained with AAA19983 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTCD1 Antibody (AAA19983, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of PTCD1 using anti-PTCD1 antibody (AAA19983).PTCD1 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PTCD1 Antibody (AAA19983) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human SIHA whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat heart tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTCD1 antigen affinity purified polyclonal antibody (#AAA19983) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTCD1 at approximately 79 kDa. The expected band size for PTCD1 is at 79 kDa.)

PTCD1, Polyclonal Antibody (Cat# AAA19983)

Full Name

Anti-PTCD1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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Caspase-7, Polyclonal Antibody (Cat# AAA30784)

Full Name

Caspase-7 Rabbit Polyclonal Antibody

Gene Names

CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3

Reactivity

Human, Rat, Mouse

Applications

WB, IHC-P, IF, ICC, EIA

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Cyclin B1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Cyclin B1, Polyclonal Antibody (Cat# AAA22301)

Full Name

Cyclin B1 Polyclonal Antibody

Gene Names

CCNB1; CCNB

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human brain section using Pink 1 (AAA28759). AAA28759 was diluted at 1:1000 dilution. A undiluted binotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining)

S100B, Polyclonal Antibody (Cat# AAA28759)

Full Name

S100B Antibody

Gene Names

S100B; NEF; S100; S100-B; S100beta

Reactivity

Human, Mouse, Rat (Predicted: Rabbit)

Applications

WB, IHC-P-Leica, FC/FACS, IHC, EIA

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using HSP90AA1 antibody at dilution of 1:200 .)

HSP90AA1, Polyclonal Antibody (Cat# AAA30556)

Full Name

HSP90AA1 Polyclonal Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of THP-1 cells using anti-QARS1 antibody (AAA19879).Overlay histogram showing THP-1 cells stained with AAA19879 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-QARS1 Antibody (AAA19879, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 6. IF analysis of QARS1 using anti-QARS1 antibody (AAA19879).QARS1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-QARS1 Antibody (AAA19879) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: rat RH35 whole cell lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-QARS1 antigen affinity purified polyclonal antibody (#AAA19879) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for QARS1 at approximately 88 kDa. The expected band size for QARS1 is at 88 kDa.)

QARS1, Polyclonal Antibody (Cat# AAA19879)

Full Name

Anti-QARS1 Antibody Picoband

Gene Names

QARS; GLNRS; MSCCA; PRO2195

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HNRNPD at 1:1000 dilution.)

HNRNPD, Polyclonal Antibody (Cat# AAA30631)

Full Name

HNRNPD Rabbit Polyclonal Antibody

Gene Names

HNRNPD; P37; AUF1; AUF1A; HNRPD; hnRNPD0

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

R-Ras, Polyclonal Antibody (Cat# AAA31465)

Full Name

Phospho-R-Ras (Tyr66) Antibody

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Sheep (100%), Dog (100%)

Applications

WB, IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

OPN-a/b, Polyclonal Antibody (Cat# AAA26864)

Full Name

OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 750)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human

Applications

WB, IHC, IF

Purity

Purified by Protein A and Peptide Affinity Chromatography.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of MRC1 using anti-MRC1 antibody.)

Mannose Receptor/MRC1, Polyclonal Antibody (Cat# AAA19199)

Full Name

Anti-Mannose Receptor/MRC1 Antibody

Gene Names

MRC1; MMR; hMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)

Protein Kinase C Iota, Polyclonal Antibody (Cat# AAA21029)

Full Name

Protein Kinase C Iota (PKCi) Polyclonal Antibody

Gene Names

PRKCI; PKCI; DXS1179E; nPKC-iota

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, EIA

Purity

Affinity Chromatography

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using [KO Validated] SOD2 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

SOD2, Polyclonal Antibody (Cat# AAA28427)

Full Name

SOD2 Rabbit pAb

Gene Names

ECE1; ECE

Reactivity

Human, Mouse, Rat

Applications

IF, ICC

Purity

Affinity purification

Pricing

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WB (Western Blot) (WB Suggested Anti-BECN1 Antibody Titration: 1 ug/mlPositive Control: HepG2 cell lysate)

BECN1, Polyclonal Antibody (Cat# AAA23584)

Full Name

BECN1 antibody - N-terminal region

Gene Names

BECN1; ATG6; VPS30; beclin1

Reactivity

Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat

Applications

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PARP1 antibody.)

PARP1, Polyclonal Antibody (Cat# AAA29790)

Full Name

PARP1 Polyclonal Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP, ChIP

Purity

Affinity purification

Pricing

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Histone H3, Polyclonal Antibody (Cat# AAA29037)

Full Name

Histone H3 Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

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WB (Western Blot) (Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

PAK1, Polyclonal Antibody (Cat# AAA31096)

Full Name

PAK1 Antibody

Gene Names

PAK1; PAKalpha

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

VPX, Polyclonal Antibody (Cat# AAA27022)

Full Name

VPX Antibody

Reactivity

Human immunodeficiency virus type 2 subtype A

Applications

EIA, WB

Purity

>95%, Protein G purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using HSP90AA1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

HSP90AA1, Polyclonal Antibody (Cat# AAA22176)

Full Name

HSP90AA1 Polyclonal Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

HSP 90, Polyclonal Antibody (Cat# AAA28846)

Full Name

HSP 90 (Acetyl Lys292/284) Polyclonal Antibody

Gene Names

HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

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WB (Western Blot) (Western blot analysis of Phospho-Zyxin (Tyr316) in lysates of Jurkat paclitaxel 1uM 24h, using Phospho-Zyxin (Tyr316) Antibody.)

Zyxin, Polyclonal Antibody (Cat# AAA31158)

Full Name

Phospho-Zyxin (Tyr316) Antibody

Gene Names

ZYX; ESP-2; HED-2

Reactivity

Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken

Applications

WB, IHC, EIA

Purity

Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using TGFB1 Rabbit pAb.)

TGFB1, Polyclonal Antibody (Cat# AAA30644)

Full Name

TGFB1 Rabbit Polyclonal Antibody

Gene Names

TGFB1; CED; LAP; DPD1; TGFB; TGFbeta

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.

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Polyclonal Antibodies

Viewing 1000-1050 of 3176 product results

TMPO, Polyclonal Antibody (Cat# AAA29699)

Akt, Polyclonal Antibody (Cat# AAA28964)

beta Catenin, Polyclonal Antibody (Cat# AAA29797)

p53, Polyclonal Antibody (Cat# AAA31354)

TARDBP, Polyclonal Antibody (Cat# AAA30659)

PFKFB1, Polyclonal Antibody (Cat# AAA19903)

MEK-1/2, Polyclonal Antibody (Cat# AAA29065)

HNRNPA1, Polyclonal Antibody (Cat# AAA29846)

eIF-4E, Polyclonal Antibody (Cat# AAA29880)

NADPH Oxidase 4, Polyclonal Antibody (Cat# AAA29878)

p53, Polyclonal Antibody (Cat# AAA31429)

CEBPG, Polyclonal Antibody (Cat# AAA28103)

TOMM20, Polyclonal Antibody (Cat# AAA28123)

delta 1 Catenin/CAS/CTNND1, Polyclonal Antibody (Cat# AAA19453)

PIK3C2A, Polyclonal Antibody (Cat# AAA22280)

Stat-1 alpha/beta, Polyclonal Antibody (Cat# AAA29957)

UMPS, Polyclonal Antibody (Cat# AAA28172)

AIFM2, Polyclonal Antibody (Cat# AAA11045)

Caspase3, Polyclonal Antibody (Cat# AAA29796)

CDA, Polyclonal Antibody (Cat# AAA28268)

DYNLL1, Polyclonal Antibody (Cat# AAA28275)

p300, Polyclonal Antibody (Cat# AAA29317)

HBcAg (HBV), Polyclonal Antibody (Cat# AAA13719)

vvhA, Polyclonal Antibody (Cat# AAA27021)

NY-CO-9, Polyclonal Antibody (Cat# AAA29084)

ERK1/ERK2, Polyclonal Antibody (Cat# AAA22198)

BCL2, Polyclonal Antibody (Cat# AAA29670)

VDAC3, Polyclonal Antibody (Cat# AAA19301)

IDH1, Polyclonal Antibody (Cat# AAA28676)

PTCD1, Polyclonal Antibody (Cat# AAA19983)

Caspase-7, Polyclonal Antibody (Cat# AAA30784)

Cyclin B1, Polyclonal Antibody (Cat# AAA22301)

S100B, Polyclonal Antibody (Cat# AAA28759)

HSP90AA1, Polyclonal Antibody (Cat# AAA30556)

QARS1, Polyclonal Antibody (Cat# AAA19879)

HNRNPD, Polyclonal Antibody (Cat# AAA30631)

R-Ras, Polyclonal Antibody (Cat# AAA31465)

OPN-a/b, Polyclonal Antibody (Cat# AAA26864)

Mannose Receptor/MRC1, Polyclonal Antibody (Cat# AAA19199)

Protein Kinase C Iota, Polyclonal Antibody (Cat# AAA21029)

SOD2, Polyclonal Antibody (Cat# AAA28427)

BECN1, Polyclonal Antibody (Cat# AAA23584)

PARP1, Polyclonal Antibody (Cat# AAA29790)

Histone H3, Polyclonal Antibody (Cat# AAA29037)

PAK1, Polyclonal Antibody (Cat# AAA31096)

VPX, Polyclonal Antibody (Cat# AAA27022)

HSP90AA1, Polyclonal Antibody (Cat# AAA22176)

HSP 90, Polyclonal Antibody (Cat# AAA28846)

Zyxin, Polyclonal Antibody (Cat# AAA31158)

TGFB1, Polyclonal Antibody (Cat# AAA30644)

What are Polyclonal Antibodies?

Key Uses of Polyclonal Antibodies

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

2. Rigorous Quality Control

3. Wide Assortment of Antibodies

4. Highly Purified

FAQ

1. How are polyclonal antibodies produced?

2. How do polyclonal antibodies differ from monoclonal antibodies?

3. How should I store polyclonal antibodies?

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