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▼Polyclonal Antibodies
At AAA Biotech also known as AAA Bio or AAABio, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
Viewing 1000-1050 of 96811 product results
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
DRP1, Polyclonal Antibody (Cat# AAA29014)
Full Name
DRP1 Polyclonal Antibody
Gene Names
DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
Application Data
mTOR, Polyclonal Antibody (Cat# AAA14086)
Full Name
Rabbit anti mTOR Polyclonal antibody
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HL-60 cells using anti-Sumo 1/SUMO1 antibody (AAA19224).Overlay histogram showing HL-60 cells stained with AAA19224 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sumo 1/SUMO1 Antibody (AAA19224, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Sumo 1/SUMO1, Polyclonal Antibody (Cat# AAA19224)
Full Name
Anti-Sumo 1/SUMO1 Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using WNT5B at dilution of 1:100 (40x lens).)
WNT5B, Polyclonal Antibody (Cat# AAA30703)
Full Name
WNT5B Rabbit Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H2A, Polyclonal Antibody (Cat# AAA31347)
Full Name
Acetyl-Histone H2A (Lys5) Antibody
Gene Names
HIST1H2AB; H2A/m; H2AFM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
Transfection Control
(Anti-GPR182 / ADMR antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)
GPR182 / ADMR, Polyclonal Antibody (Cat# AAA12333)
Full Name
Rabbit Polyclonal to Human GPR182 / ADMR
Gene Names
GPR182; AMR; 7TMR; ADMR; AM-R; G10D; gamrh; hrhAMR
Reactivity
Gorilla, Human, Monkey
Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
Predicted Reactivity: Gibbon (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Immunocytochemistry
Purity
Immunoaffinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using p70 S6 Kinase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
p70 S6 Kinase 1, Polyclonal Antibody (Cat# AAA22303)
Full Name
p70 S6 Kinase 1 Polyclonal Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
USF1, Polyclonal Antibody (Cat# AAA31437)
Full Name
Phospho-USF1 (Thr153) Antibody
Gene Names
USF1; UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)
NOS3, Polyclonal Antibody (Cat# AAA29082)
Full Name
NOS3 Polyclonal Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of lysates from Jurkat cells treated with PMA 125ng/ml 30' and Jurkat cells treated with insulin 0.01U/ml 15', using Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody. The lane on the right is blocked with the phospho peptide.)
Retinoic Acid Receptor alpha, Polyclonal Antibody (Cat# AAA30738)
Full Name
Retinoic Acid Receptor alpha (Phospho-Ser77) Antibody
Gene Names
RARA; RAR; NR1B1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified from rabbit antiserum by affinity-chromatographyusing phospho peptide. The antibody against non-phospho peptide wasremoved by chromatography using corresponding non-phospho peptide.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MAP1LC3A at 1:1000 dilution.)
MAP1LC3A, Polyclonal Antibody (Cat# AAA30594)
Full Name
MAP1LC3A Rabbit Polyclonal Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human liver damage using COX5B at dilution of 1:100 (40x lens).)
COX5B, Polyclonal Antibody (Cat# AAA30666)
Full Name
COX5B Rabbit Polyclonal Antibody
Gene Names
COX5B; COXVB
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western Blot using AAA14722 shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70kD corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead).)
LINE-1, Polyclonal Antibody (Cat# AAA14722)
Full Name
LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2)
Gene Names
L1TD1; ECAT11; RP5-1155K23.3
Reactivity
Human
Applications
Western Blot
Purity
Purified
Purified by selective precipitation and salt fractionation followed by extensive dialysis.
Purified by selective precipitation and salt fractionation followed by extensive dialysis.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U2OS cells using anti-Filamin B/FLNB antibody (AAA19463).Overlay histogram showing U2OS cells stained with AAA19463 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Filamin B/FLNB Antibody (AAA19463, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Filamin B/FLNB, Polyclonal Antibody (Cat# AAA19463)
Full Name
Anti-Filamin B/FLNB Antibody Picoband
Gene Names
FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of 293T cells using anti-PTCD1 antibody (AAA19983).Overlay histogram showing 293T cells stained with AAA19983 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTCD1 Antibody (AAA19983, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PTCD1, Polyclonal Antibody (Cat# AAA19983)
Full Name
Anti-PTCD1 Antibody Picoband
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Human Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Human COL4a4 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog:))
Collagen Type IV Alpha 4, Polyclonal Antibody (Cat# AAA21020)
Full Name
Collagen Type IV Alpha 4 (COL4a4) Polyclonal Antibody
Gene Names
COL4A4; CA44
Reactivity
Human
Applications
Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Mouse kidney using KRT14 antibody at dilution of 1:100 .)
KRT14, Polyclonal Antibody (Cat# AAA30574)
Full Name
KRT14 Polyclonal Antibody
Gene Names
KRT14; K14; NFJ; CK14; EBS3; EBS4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using ABCB7 antibody at dilution of 1:100 (40x lens).)
ABCB7, Polyclonal Antibody (Cat# AAA28279)
Full Name
ABCB7 Polyclonal Antibody
Gene Names
ABCB7; ABC7; ASAT; Atm1p; EST140535
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
ICC (Immunocytochemistry)
(ICC staining HSP70 in SKBR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
HSP70, Polyclonal Antibody (Cat# AAA29967)
Full Name
HSP70 Antibody
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
Peptide affinity purified
Pricing
IHC (Immunohistchemistry)
(Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)
MYH6/MYH7, Polyclonal Antibody (Cat# AAA29387)
Full Name
MYH6/MYH7 Polyclonal Antibody
Gene Names
MYH6; ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
p57, Polyclonal Antibody (Cat# AAA29226)
Full Name
p57 Polyclonal Antibody
Gene Names
CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
Inhibin beta-A, Polyclonal Antibody (Cat# AAA29247)
Full Name
Inhibin beta-A Polyclonal Antibody
Gene Names
INHBA; EDF; FRP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
AR, Polyclonal Antibody (Cat# AAA31420)
Full Name
Phospho-AR (Tyr535) Antibody
Gene Names
AR; KD; AIS; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)
Predicted Reactivity: Pig (92%), Bovine (85%), Sheep (85%), Rabbit (92%), Dog (92%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various celllines, using GSK3 alpha/beta Antibody.)
GSK3 alpha/beta, Polyclonal Antibody (Cat# AAA31094)
Full Name
GSK3 alpha/beta Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(HSPA5 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
HSPA5, Polyclonal Antibody (Cat# AAA28738)
Full Name
HSPA5 Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
IRS-1, Polyclonal Antibody (Cat# AAA28930)
Full Name
IRS-1 (phospho Ser636) Polyclonal Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Ku-70, Polyclonal Antibody (Cat# AAA28848)
Full Name
Ku-70 (Acetyl Lys542) Polyclonal Antibody
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD85g, Polyclonal Antibody (Cat# AAA29200)
Full Name
CD85g Polyclonal Antibody
Gene Names
LILRA4; ILT7; CD85g
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U87 cells using anti-Nesprin3/SYNE3 antibody (AAA19982).Overlay histogram showing U87 cells stained with AAA19982 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nesprin3/SYNE3 Antibody (AAA19982, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Nesprin3/SYNE3, Polyclonal Antibody (Cat# AAA19982)
Full Name
Anti-Nesprin3/SYNE3 Antibody Picoband
Gene Names
SYNE3; NET53; Nesp3; C14orf49
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
OTUB1, Polyclonal Antibody (Cat# AAA31284)
Full Name
Phospho-OTUB1 (Ser16) Antibody
Gene Names
OTUB1; OTB1; OTU1; HSPC263
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-BIK antibody (AAA11666).Overlay histogram showing A431 cells stained with AAA11666 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (AAA11666,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Bik, Polyclonal Antibody (Cat# AAA11666)
Full Name
Anti-Bik Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using [KO Validated] TFEB Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
TFEB, Polyclonal Antibody (Cat# AAA28425)
Full Name
TFEB Rabbit pAb
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of C9JLR9 Antibody (C-term) (AAA28790) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
C9JLR9, Polyclonal Antibody (Cat# AAA28790)
Full Name
C9JLR9 Antibody (C-term)
Reactivity
Human
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using FoxO3a antibody at 1:1000 dilution.)
FoxO3a, Polyclonal Antibody (Cat# AAA30541)
Full Name
FoxO3a Polyclonal Antibody
Gene Names
Foxo3; Fkhr2; C76856; FKHRL1; Foxo3a; 1110048B16Rik; 2010203A17Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of A-549 cells, using EGFR antibody.)
EGFR, Polyclonal Antibody (Cat# AAA30593)
Full Name
EGFR Rabbit Polyclonal Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using SNAI2 antibody. Blue: DAPI for nuclear staining.)
SNAI2, Polyclonal Antibody (Cat# AAA10674)
Full Name
SNAI2 Polyclonal Antibody
Gene Names
SNAI2; SLUG; WS2D; SLUGH1; SNAIL2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Figure 13. IHC analysis of EEF2/Elongation factor 2 using anti-EEF2/Elongation factor 2 antibody (AAA19229).EEF2/Elongation factor 2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-EEF2/Elongation factor 2 Antibody (AAA19229) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
EEF2/Elongation factor 2, Polyclonal Antibody (Cat# AAA19229)
Full Name
Anti-EEF2/Elongation factor 2 Antibody
Gene Names
EEF2; EF2; EF-2; EEF-2
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using PTPIP51/RMDN3 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)
PTPIP51/RMDN3, Polyclonal Antibody (Cat# AAA22290)
Full Name
PTPIP51/RMDN3 Polyclonal Antibody
Gene Names
RMDN3; RMD3; RMD-3; FAM82C; FAM82A2; ptpip51
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(WB Suggested Anti-HNF4A antibody Titration: 1 ug/mLSample Type: Human Liver)
HNF4A, Polyclonal Antibody (Cat# AAA23401)
Full Name
HNF4A Antibody - N-terminal region
Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IDH1, Polyclonal Antibody (Cat# AAA28676)
Full Name
IDH1 Antibody (Center)
Gene Names
IDH1; IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.)
Rab 26, Polyclonal Antibody (Cat# AAA29142)
Full Name
Rab 26 Polyclonal Antibody
Gene Names
RAB26; V46133
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)
TERT, Polyclonal Antibody (Cat# AAA29282)
Full Name
TERT Polyclonal Antibody
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
ICC (Immunocytochemistry)
(ICC staining FBXL2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FBXL2, Polyclonal Antibody (Cat# AAA29885)
Full Name
FBXL2 Antibody
Gene Names
FBXL2; FBL2; FBL3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
Histone H3, Polyclonal Antibody (Cat# AAA28841)
Full Name
Histone H3 (Di Methyl Lys5) Polyclonal Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using PD-1 antibody.)
PD-1, Polyclonal Antibody (Cat# AAA29800)
Full Name
PD-1 Polyclonal Antibody
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)
CD241, Polyclonal Antibody (Cat# AAA29204)
Full Name
CD241 Polyclonal Antibody
Gene Names
RHAG; RH2; Rh50; CD241; RH50A; Rh50GP; SLC42A1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Pricing
IHC (Immunohistochemistry)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)
CD63, Polyclonal Antibody (Cat# AAA29287)
Full Name
CD63 Polyclonal Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using PDPK1 antibody. Blue: DAPI for nuclear staining.)
PDPK1, Polyclonal Antibody (Cat# AAA10678)
Full Name
PDPK1 Polyclonal Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
LIMK1, Polyclonal Antibody (Cat# AAA31425)
Full Name
Phospho-LIMK1 (Ser310) Antibody
Gene Names
LIMK1; LIMK; LIMK-1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (88%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (88%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat Testis labeling TMPRSS2 with 9569 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
TMPRSS2, Polyclonal Antibody (Cat# AAA11037)
Full Name
TMPRSS2 (CT) Antibody
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
Predicted species reactivity based on immunogen sequence: Monkey (100%); Gorilla (100%); Cat (92%).
Applications
Immunofluorescence, Western Blot
Purity
TMPRSS2 Antibody is affinity chromatography purified via peptide column.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
- Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
- Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
- ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
- Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
- Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.