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▼Recombinant Antibodies
Also available for purchase in our catalog, we have flexible recombinant antibodies that are excellent tools in many different types of experiments, and well-suited for those that are large-scale or long-term. Our collection of recombinant antibodies includes different forms/formats of said antibodies, such as labeled or purified versions, and can be made utilizing different species’ isotypes to suit various experimental requirements.
Viewing 450-500 of 3337 product results
IHC (Immunohiostchemistry)
(IHC image of AAA279319 diluted at 1?150 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.)
AOC3, Monoclonal Recombinant Antibody (Cat# AAA279319)
Full Name
AOC3 Recombinant Monoclonal Antibody
Gene Names
AOC3; HPAO; SSAO; VAP1; VAP-1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity-chromatography
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279325 diluted at 1?100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.)
SOST, Monoclonal Recombinant Antibody (Cat# AAA279325)
Full Name
SOST Recombinant Monoclonal Antibody
Gene Names
SOST; CDD; VBCH
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity-chromatography
Pricing
ELISA
(The Binding Activity of MAPT with Anti-MAPT recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta MAPT at 2 ug/ml can bind Anti-MAPT recombinant antibody, the EC50 is 2.464-3.979 ng/ml.)
MAPT, Monoclonal Recombinant Antibody (Cat# AAA279016)
Full Name
MAPT Recombinant Monoclonal Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
Reactivity
Human
Applications
ELISA
Purity
Affinity purified
Pricing
ELISA
(The Binding Activity of Huamn TROP2 with Anti-TROP2 recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human TROP2 at 2 ug/mL can bind Anti-TROP2 recombinant antibody, the EC50 is 0.9108-1.640 ng/mL.)
TROP2, Monoclonal Recombinant Antibody (Cat# AAA279031)
Full Name
TROP2 Recombinant Monoclonal Antibody
Gene Names
TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Reactivity
Human
Applications
ELISA
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA278957 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
HSP90AB1, Monoclonal Recombinant Antibody (Cat# AAA278957)
Full Name
HSP90AB1 Recombinant Monoclonal Antibody
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA278969 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
RPS19, Monoclonal Recombinant Antibody (Cat# AAA278969)
Full Name
RPS19 Recombinant Monoclonal Antibody
Gene Names
RPS19; DBA; S19; DBA1
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA278971 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
HIF1AN, Monoclonal Recombinant Antibody (Cat# AAA278971)
Full Name
HIF1AN Recombinant Monoclonal Antibody
Gene Names
HIF1AN; FIH1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA278974 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
OS9, Monoclonal Recombinant Antibody (Cat# AAA278974)
Full Name
OS9 Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Histone H2A (Acetyl-K9) staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
Histone H2A, Monoclonal Recombinant Antibody (Cat# AAA266306)
Full Name
Recombinant Anti-Histone H2A (Acetyl-K9) Rabbit mAb
Gene Names
HIST1H2AB; H2A/m; H2AFM
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD10 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CD10, Monoclonal Recombinant Antibody (Cat# AAA266246)
Full Name
Recombinant Anti-CD10 Rabbit mAb
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of IGFBP7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
IGFBP7, Monoclonal Recombinant Antibody (Cat# AAA266279)
Full Name
Recombinant Anti-IGFBP7 Rabbit mAb
Gene Names
IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Rab 9 p40 staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
Rab 9 p40, Monoclonal Recombinant Antibody (Cat# AAA266282)
Full Name
Recombinant Anti-Rab 9 p40 Rabbit mAb
Gene Names
RABEPK; p40; RAB9P40; bA65N13.1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of TRPV5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
TRPV5, Monoclonal Recombinant Antibody (Cat# AAA266285)
Full Name
Recombinant Anti-TRPV5 Rabbit mAb
Gene Names
TRPV5; CAT2; ECAC1; OTRPC3
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
Neu5Ac/O-sialic acid/Polysialic acid, Monoclonal Recombinant Antibody (Cat# AAA120323)
Full Name
Anti-Neu5Ac/O-sialic acid/Polysialic acid Antibody (Ab735)
Reactivity
General
Applications
Western Blot, Flow Cytometry
Purity
>95% by SDS-PAGE
Protein A or G purified from cell culture supernatant.
Protein A or G purified from cell culture supernatant.
Pricing
IHC (Immunohiostchemistry)
(IHC image of AAA279085 diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.34% DAB.)
KRT10, Monoclonal Recombinant Antibody (Cat# AAA279085)
Full Name
KRT10 Recombinant Monoclonal Antibody
Gene Names
KRT10; BIE; EHK; K10; KPP; BCIE; CK10
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279119 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
MAPK1/MAPK3, Monoclonal Recombinant Antibody (Cat# AAA279119)
Full Name
MAPK1/MAPK3 Recombinant Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing 293 cells stained with AAA279153 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,026 events was performed.)
ANPEP, Monoclonal Recombinant Antibody (Cat# AAA279153)
Full Name
ANPEP Recombinant Monoclonal Antibody
Gene Names
ANPEP; APN; CD13; LAP1; P150; PEPN; GP150
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279176 (red line) at 1:800. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,017 events was performed.)
ANXA4, Monoclonal Recombinant Antibody (Cat# AAA279176)
Full Name
ANXA4 Recombinant Monoclonal Antibody
Gene Names
ANXA4; ANX4; PIG28; ZAP36; HEL-S-274
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Untransfected MC38 cells (green line) and transfected Human CLDN6 MC38 stable cells (red line) were stained with anti-CLDN6 antibody (2ug/1*106cells), washed and then followed by APC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
CLDN6, Monoclonal Recombinant Antibody (Cat# AAA279301)
Full Name
CLDN6 Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry
Purity
Affinity-chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279006 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SRR, Monoclonal Recombinant Antibody (Cat# AAA279006)
Full Name
SRR Recombinant Monoclonal Antibody
Gene Names
SRR; ILV1; ISO1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Untransfected CHO-K1 cells (green line) and transfected Human CCR8 CHO-K1 stable cells (red line) were stained with anti-CCR8 recombinant antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
CCR8, Monoclonal Recombinant Antibody (Cat# AAA279026)
Full Name
CCR8 Recombinant Monoclonal Antibody
Gene Names
CCR8; CY6; TER1; CCR-8; CKRL1; CDw198; CMKBR8; GPRCY6; CMKBRL2; CC-CKR-8
Reactivity
Human
Applications
Flow Cytometry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA278979 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ITGA6, Monoclonal Recombinant Antibody (Cat# AAA278979)
Full Name
ITGA6 Recombinant Monoclonal Antibody
Gene Names
ITGA6; CD49f; VLA-6; ITGA6B
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of SAP61 staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
SAP61, Monoclonal Recombinant Antibody (Cat# AAA266327)
Full Name
Recombinant Anti-SAP61 Rabbit mAb
Gene Names
SF3A3; PRP9; PRPF9; SAP61; SF3a60
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of SMC1A staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
SMC1A, Monoclonal Recombinant Antibody (Cat# AAA266277)
Full Name
Recombinant Anti-SMC1A Rabbit mAb
Gene Names
SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD168 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CD168, Monoclonal Recombinant Antibody (Cat# AAA266297)
Full Name
Recombinant Anti-CD168 Rabbit mAb
Gene Names
HMMR; CD168; IHABP; RHAMM
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SF2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
SF2, Monoclonal Recombinant Antibody (Cat# AAA266417)
Full Name
Recombinant Anti-SF2 Rabbit mAb
Gene Names
SRSF1; ASF; SF2; SFRS1; SF2p33; SRp30a
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from serum-starved NIH/3T3, treated with UV (1h), using Phospho-c-Jun (Ser73) Antibody, Rabbit Mab (#AAA259510)(Sino Biological) and other brands' antibodies (Company C) at dilution of 1:5000, 1:20000 and 1:100000.)
c-Jun, Monoclonal Recombinant Antibody (Cat# AAA259510)
Full Name
Phospho-c-Jun (Ser73) Antibody, Rabbit MAb
Reactivity
Human
Applications
Western Blot
Purity
Protein A
Pricing
IHC (Immunohistochemisry)
(IHC image of AAA278963 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)
ALCAM, Monoclonal Recombinant Antibody (Cat# AAA278963)
Full Name
ALCAM Recombinant Monoclonal Antibody
Gene Names
ALCAM; MEMD; CD166
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing SH-SY5Y cells stained with AAA279075 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
EGR2, Monoclonal Recombinant Antibody (Cat# AAA279075)
Full Name
EGR2 Recombinant Monoclonal Antibody
Gene Names
EGR2; AT591; CMT1D; CMT4E; KROX20
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA279098 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
GPI, Monoclonal Recombinant Antibody (Cat# AAA279098)
Full Name
GPI Recombinant Monoclonal Antibody
Gene Names
GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279056 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
TK1, Monoclonal Recombinant Antibody (Cat# AAA279056)
Full Name
TK1 Recombinant Monoclonal Antibody
Gene Names
TK1; TK2
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279067 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
RELA, Monoclonal Recombinant Antibody (Cat# AAA279067)
Full Name
RELA Recombinant Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing SH-SY5Y cells stained with AAA279146 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SNCA, Monoclonal Recombinant Antibody (Cat# AAA279146)
Full Name
SNCA Recombinant Monoclonal Antibody
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing JK cells stained with AAA279167 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,002 events was performed.)
GJB2, Monoclonal Recombinant Antibody (Cat# AAA279167)
Full Name
GJB2 Recombinant Monoclonal Antibody
Gene Names
GJB2; HID; KID; PPK; CX26; DFNA3; DFNB1; NSRD1; DFNA3A; DFNB1A
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing MCF7 cells stained with AAA279171 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,011 events was performed.)
ITGAV, Monoclonal Recombinant Antibody (Cat# AAA279171)
Full Name
ITGAV Recombinant Monoclonal Antibody
Gene Names
ITGAV; CD51; MSK8; VNRA; VTNR
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279312 at 1:80, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Human IgG(H+L).)
SDC1, Monoclonal Recombinant Antibody (Cat# AAA279312)
Full Name
SDC1 Recombinant Monoclonal Antibody
Gene Names
SDC1; SDC; CD138; SYND1; syndecan
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity-chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Thymidylate Synthase staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).)
Thymidylate Synthase, Monoclonal Recombinant Antibody (Cat# AAA266413)
Full Name
Recombinant Anti-Thymidylate Synthase Rabbit mAb
Gene Names
TYMS; TS; TMS; HST422
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ACC alpha/beta staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
ACC alpha/beta, Monoclonal Recombinant Antibody (Cat# AAA266276)
Full Name
Recombinant Anti-ACC alpha/beta Rabbit mAb
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of PIN4 staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
PIN4, Monoclonal Recombinant Antibody (Cat# AAA266288)
Full Name
Recombinant Anti-PIN4 Rabbit mAb
Gene Names
PIN4; EPVH; PAR14; PAR17
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
ELISA
(The Binding Activity of Macaca fascicularis DPEP3 with Anti-DPEP3 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis DPEP3 (CSB-MP007125MOV) at 2 ug/mL can bind Anti-DPEP3 recombinant antibody. The EC50 is 7.817-8.936 ng/mL.)
DPEP3, Monoclonal Recombinant Antibody (Cat# AAA279300)
Full Name
DPEP3 Recombinant Monoclonal Antibody
Gene Names
DPEP3; MBD3
Reactivity
Human, Macaca fascicularis
Applications
ELISA
Purity
Affinity-chromatography
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279147 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
MRAS, Monoclonal Recombinant Antibody (Cat# AAA279147)
Full Name
MRAS Recombinant Monoclonal Antibody
Gene Names
MRAS; M-RAs; RRAS3; R-RAS3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279078 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
H2AFX, Monoclonal Recombinant Antibody (Cat# AAA279078)
Full Name
H2AFX Recombinant Monoclonal Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry
Purity
Affinity purified
Pricing
ELISA
(The Binding Activity of Human FOLR1 with Anti-FOLR1 recombinant antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized human FOLR1(CSB-MP008784HU1) at 2 ug/mL can bind Anti-FOLR1 recombinant antibody. The EC50 is 6.893-7.883 ng/mL.)
FOLR1, Monoclonal Recombinant Antibody (Cat# AAA279308)
Full Name
FOLR1 Recombinant Monoclonal Antibody
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human, Cynomolgus
Applications
ELISA
Purity
Affinity-chromatography
Pricing
Bioactivity
(The Binding Activity of Human LTBR with Anti-LTBR Recombinant AntibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human LTBR at 2ug/mL can bind Anti-LTBR recombinant antibody. The EC50 is 0.5282-0.6120ng/mL.)
LTBR, Monoclonal Recombinant Antibody (Cat# AAA279346)
Full Name
LTBR Recombinant Monoclonal Antibody
Gene Names
LTBR; CD18; TNFCR; TNFR3; D12S370; TNFR-RP; TNFRSF3; TNFR2-RP; LT-BETA-R; TNF-R-III
Reactivity
Human
Applications
ELISA
Purity
Affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Jurkat cells stained with AAA279351 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100.Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4 degree C.Control antibody (green line) was human IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
ILDR2, Monoclonal Recombinant Antibody (Cat# AAA279351)
Full Name
ILDR2 Recombinant Monoclonal Antibody
Gene Names
ILDR2; C1orf32; dJ782G3.1
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence
Purity
Affinity chromatography
Pricing
FCM/FACS (Flow Cytometry)
(Untransfected HEK293T cells (green line) and transfected Human CLDN6 HEK293T stable cells (red line) were stained with anti-CLDN6/9 recombinant antibody (2ug/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.)
CLDN6/9, Monoclonal Recombinant Antibody (Cat# AAA279025)
Full Name
CLDN6/9 Recombinant Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry
Purity
Affinity purified
Pricing
ELISA
(The Binding Activity of Macaca fascicularis TPBG with Anti-TPBG monoclonal antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TPBG at 2 ug/mL can bind Anti-TPBG recombinant antibody, the EC50 is 1.672-2.181 ng/mL.)
TPBG, Monoclonal Recombinant Antibody (Cat# AAA279029)
Full Name
TPBG Recombinant Monoclonal Antibody
Gene Names
TPBG; 5T4; M6P1; 5T4AG
Reactivity
Human
Applications
ELISA
Purity
Affinity purified
Pricing
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing HepG2 cells stained with AAA279066 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
SLC2A2, Monoclonal Recombinant Antibody (Cat# AAA279066)
Full Name
SLC2A2 Recombinant Monoclonal Antibody
Gene Names
SLC2A2; GLUT2
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Affinity purified
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CD68 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
CD68, Monoclonal Recombinant Antibody (Cat# AAA266256)
Full Name
Recombinant Anti-CD68 Rabbit mAb
Gene Names
CD68; GP110; LAMP4; SCARD1
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of Fibrillarin staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).)
Fibrillarin, Monoclonal Recombinant Antibody (Cat# AAA266266)
Full Name
Recombinant Anti-Fibrillarin Rabbit mAb
Gene Names
FBL; FIB; FLRN; RNU3IP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
What are recombinant antibodies?
Recombinant antibodies are produced in the lab by utilizing a known genetic sequence (an amino acid and nucleotide sequence). This allows for high consistency in how each new lot will perform and how specific they will be for the target, regardless of how many times they are produced/reproduced.
They help solve the problems observed when traditional non-recombinant techniques are utilized, such as changes in cell behavior over time, differences between production batches, and accuracy in complex biological systems.
A powerful feature of recombinant antibodies is that they can also be modified through progressive genetic engineering and subsequent testing in order to make them perform better and/or more reliably.
Applications For Recombinant Antibodies
1. Basic Research
Recombinant antibodies are primarily utilized in laboratory experiments to study the function of various proteins in cells. They are helpful in finding and tracking specific molecules through their use in techniques such as ELISA, Western blotting, immunofluorescence, and flow cytometry.
2. Diagnostics
They are also key components in diagnostic tests used to detect diseases and infections. As recombinant antibodies are produced in a consistent and controlled way, they are able to provide highly reliable results across batches. Though recombinant antibodies are indeed quite useful in diagnostic applications, all of the products listed in AAA Biotech’s also known as AAA Bio or AAABio catalog are strictly for research-use only (RUO).
3. Therapeutics (Medical Treatments)
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
- Chimeric Antibodies: Commonly used in therapeutic treatment. They combine different pieces of mouse and human antibodies in order to reduce immune reactions.
- Humanized Antibodies: Modified non-human antibodies that are made to look the same as actual native human antibodies, making them safer and more effective.
- Antibody Fragments (Fab, scFv, VHH): Smaller pieces of antibody fragments that can reach deep into tissues and tumors. They are useful in cancer treatments and imaging.
- Bispecific Antibodies: Specially-engineered antibodies that can bind to two different target molecules at once. Each antibody has two “arms” that allow for two places where the target antigen can bind (both “arms” will recognize the same exact epitope). These bispecific antibodies are engineered so that each arm can detect a completely different epitope than the other. Used in many of the treatments for cancer, diabetes, Alzheimer’s disease, and more.
- Fc-Fusion Proteins: Used as drugs and research tools. They combine parts of antibodies with other proteins to improve stability and effectiveness.
4. Imaging and Detection
Small antibody fragments are also used as imaging agents in medical scans. Their small size helps them reach and report on the status of tumors quickly.
5. Drug Development and Delivery
Recombinant antibodies can also be designed and made to deliver drugs directly to diseased cells, especially in targeted cancer therapy.
6. Immunotherapy
These antibodies can aid the immune system recognize and fight diseases more effectively, which is often their role in cancer treatment.
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Advantages of Recombinant Antibodies
- Reliable Results Every Time: Recombinant antibodies are exceptionally consistent from batch to batch. This means researchers can trust that results will remain the same each time they purchase and use them.
- More Specific and Accurate: Compared to polyclonal antibodies, recombinant antibodies are significantly less likely to exhibit non-specific binding, which promotes more accurate and clearer results.
- Easier to Develop: Making recombinant antibodies is simpler and quicker than the traditional method of producing monoclonal antibodies via hybridomas.
- No Genetic Drift: Over time, hybridoma cells can change or become unstable, which can result in the antibodies progressively losing their function/specificity. Recombinant antibodies avoid this problem because they are produced from a fixed template DNA sequence.
- Truly Monoclonal: Studies have shown that many hybridomas thought to be monoclonal actually are not so, which can lead to, in some cases, unreliable results. Recombinant antibodies are made using precise genetic sequence templates, ensuring they are truly specific to one target.
- Long-Term Stability and Safety: Since their amino acid and genetic sequences are known and stored, recombinant antibodies can be reproduced at any time with the same quality. This method protects against contamination, genetic changes over time, or even accidental loss/deterioration of cell lines.
Why Buy Recombinant Antibody from AAA Biotech?
- Highly Validated: The vast majority of our antibodies are thoroughly tested to ensure they work reliably across different applications and experimental platforms.
- Versatile Applications: Our antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).
- Support for Rare Species: We offer antibodies specifically designed for rare or less commonly used laboratory species — something many other suppliers do not provide.
- Wide Selection of Conjugated and Recombinant Forms: Choose from various labeled (conjugated) options and browse our high-quality recombinant antibodies – we are confident that we will have materials to meet your research needs.
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
FAQ
1. What are recombinant antibodies used for?
Research (e.g., ELISA, Western blot, flow cytometry)
Disease diagnostics
Therapeutic treatments (e.g., cancer, autoimmune diseases)
Medical imaging
Targeted drug delivery
Vaccine development
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
2. What is the difference between traditional and recombinant antibodies?
Traditional antibodies: Produced using animal hosts (hybridomas are typically made from an original animal inoculation), slower, less consistent, risk of genetic drift
Recombinant antibodies: Produced using DNA template sequences via laboratory cell lines, faster, highly consistent, animal-free, easily customizable
3. What are antibodies used to treat?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Antibodies are proteins that your body makes to protect you from infections, allergies, and other potentially harmful substances. Scientists have used the intrinsic properties of antibodies to develop them into tools for scientific advancement, and can now create special lab-grown antibodies that aid in the treatment of diseases such as cancer, heart-related issues, and arthritis.
4. Why do we need recombinant proteins?
Recombinant proteins are lab-made proteins that have become essential in the advancement of science and medicine. They’re used in the lab for innumerable purposes, such as to help grow and support cells, especially for tasks like expanding cell colonies, helping them develop into other types, or turning them into stem cells.